scholarly journals Three Dimensional Evaluation of Marginal Microleakage at the Adhesive Interface between Different Fissure Sealants and Enamel: Micro-CT Analysis

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Akif Demirel ◽  
Ayşe Işıl Orhan ◽  
Firdevs Tulga Öz ◽  
Kaan Orhan

Summary Background/Aim: Sealing ability and adhesion to enamel of fissure sealants are quite critical in prevention of dental caries. The objective of the present study was to assess the marginal microleakage of different material-based pit and fissures with non-invasive and invasive techniques by using the Micro-CT method. Material and Methods: 30 extracted human permanent molars were randomly assigned to three sealant groups (n=10) as resin-based ClinPro, giomer-based BeautiSealant, and glass ionomer-based Fuji IX-GP. Half of each group was randomly divided into two parts as non-invasive and invasive techniques. The volumetric analysis of marginal microleakage between the enamel-material interface was performed by using Micro-CT. The results were analyzed with Student’s t, One-Way ANOVA, Tukey and Games-Howell tests. Statistical significance level was considered as 0.05. Results: In non-invasive technique, Fuji IX-GP showed statistically significantly lower marginal microleakage values than Clinpro (p=0.022). Other comparisons were not significant (p>0.05). However, in invasive technique, microleakage values both of Fuji IX-GP and BeautiSealant were significantly lower than Clinpro (p<0.001, p<0.001). Besides, regardless of the sealant material, the invasive technique showed significantly superior results than non-invasive technique regarding marginal microleakage. Conclusions: Considering the clinical conditions, it was concluded that glass ionomer-based Fuji IX-GP and giomer-based BeautiSealant could be used as alternative to resin based sealants. Additionally, invasive technique (fissure preparation) can be recommended to reduce marginal microleakage.

2000 ◽  
Vol 24 (4) ◽  
pp. 291-297 ◽  
Author(s):  
Dhar Vineet ◽  
Shobha Tandon

In this era of preventive dentistry, many dental materials used for prevention of dental diseases are available. Since last few decades efforts are being directed towards prevention of dental caries which is one of the major dental diseases tormenting mankind. The main avenues available are plaque control, use of systemic and local fluorides and use of fissure sealants. There are many factors that contribute towards a successful sealant restoration such as properties of enamel, duration of etching, acid used for etching, manipulative variables. However, one of the prime factors governing the efficacy and life expectancy of a sealant is the marginal adaptability. This study was carried out on a total of 40 intact premolar teeth, which were divided in four main groups and subjected to evaluation under Scanning Electron Microscope. The parameters checked were the width and the number of marginal gaps. The results obtained showed that Teethmate had better marginal integrity and favored the use of invasive technique over non-invasive technique.


2019 ◽  
Vol 6 (2) ◽  
pp. 349 ◽  
Author(s):  
Rajendra Kumar ◽  
Nithin . ◽  
Sudha Rudrappa

Background: The aim of this study is to determine the success rate and safety of a non-invasive technique to obtain clean-catch midstream urine samples in newborns.Methods: Prospective bedside clinical study. After obtaining written informed consent,120 consecutive newborns admitted in NICU with no dehydration, poor feeding, need for immediate urine sample by invasive method  for whom urine collection was advised for various reasons who met the inclusion criteria were included in the study with consent being taken from the parents. After adequate milk intake supra pubic and lumbar para vertebral areas were stimulated in repeated cycles of 30 s until micturition began.Results: Success rate in obtaining a midstream urine sample within 5 min. The success rate was 90%. The mean time taken to collect urine was 64.24s, for males it was 62.55s and for females 65.93s.Conclusions: The technique has been demonstrated to be safe, quick and effective. The discomfort and time consumption usually associated with bag collection methods as well as invasive techniques can be avoided.


2018 ◽  
Vol 91 (2) ◽  
pp. 229-233 ◽  
Author(s):  
Sanket Hans Pandey ◽  
Pallav Mahesh Patni ◽  
Pradeep Jain ◽  
Arpita Chaturvedi

Introduction. Non-vital bleaching is a non-invasive technique to treat the intrinsic discoloration of teeth of several etiologies. Hydrogen peroxide and sodium perborate are commonly used bleaching agents.Aim. The aim of this case report is to demonstrate the non-vital bleaching technique in maxillary anterior teeth.Method. Maxillary central incisors were isolated with rubber dam and root canal treatment was performed. Barrier space preparation was done using a heated instrument. Glass ionomer cement was used a barrier material. Mixture of hydrogen peroxide and sodium perborate was placed in the canal and sealed with intermediate restorative material. After 1 week, the procedure was repeated to achieve the desired results.Conclusion. Non-vital bleaching is a minimally invasive procedure to restore the esthetics of a discolored non-vital tooth. However, care should be taken to prevent any post-operative complications.


Author(s):  
Stephan Achenbach

Computed tomography (CT), in the context of cardiac imaging, faces numerous challenges. The heart is a complex, three-dimensional organ, which moves very rapidly and has small dimensions. Especially the coronary arteries, the main target of cardiac CT imaging, are difficult to visualize by any non-invasive technique. Technology progress has made the use of CT for cardiac and coronary diagnosis possible. For selected applications, including ruling out coronary artery stenoses in low-risk individuals, CT has become a clinical tool. The technical progress of cardiac CT, and especially coronary CT angiography, is continuous and rapid. One major aim is to improve image quality and broaden the applicability of coronary CT angiography, while at the same time achieving lower radiation doses. The other major aim is to extract more than purely anatomic information out of the dataset and to complement the information in luminal narrowing with information on downstream ischaemia.


2020 ◽  
Vol 6 (4) ◽  
pp. 00500-2020
Author(s):  
Volker H. Schmitt ◽  
Christine Schmitt ◽  
David Hollemann ◽  
Andreas Mamilos ◽  
Willi Wagner ◽  
...  

AimLight microscopy is used as template in the evaluation and further development of medical imaging methods. Tissue shrinkage caused by histological processing is known to influence lung tissue dimensions. In diagnosis of COPD, computed tomography (CT) is widely used for automated airway measurement. The aim of this study was to compare histological and computed tomographic measurements of pig lung bronchi.MethodsAirway measurements of pig lungs were performed after freezing under controlled inflation pressure in a liquid nitrogen bath. The wall thickness of seven bronchi was measured via Micro-CT and CT using the integral-based method (IBM) and the full-width-at-half-maximum method (FWHM) automatically and histologically on frozen and paraffin sections. Statistical analysis was performed using the Wilcoxon test, Pearson's correlation coefficient with a significance level at p<0.05, scatter plots and Bland–Altman plots.ResultsBronchial wall thickness was smallest in frozen sections (median 0.71 mm) followed by paraffin sections (median 0.75 mm), Micro-CT (median 0.84 mm), and CT measurements using IBM (median 0.68 mm) and FWHM (median 1.69 mm). Statistically significant differences were found among all tested groups (p<0.05) except for CT IBM and paraffin and frozen sections and Micro-CT. There was high correlation between all parameters with statistical significance (p<0.05).ConclusionsSignificant differences in airway measurement were found among the different methods. The absolute measurements with CT IBM were closest to the histological results followed by Micro-CT, whereas CT FWHM demonstrated a distinct divergence from the other groups.


Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1094 ◽  
Author(s):  
Edward Narayan ◽  
Annabella Perakis ◽  
Will Meikle

Non-invasive techniques can be applied for monitoring the physiology and behaviour of wildlife in Zoos to improve management and welfare. Thermal imaging technology has been used as a non-invasive technique to measure the body temperature of various domesticated and wildlife species. In this study, we evaluated the application of thermal imaging to measure the body temperature of koalas (Phascolarctos cinereus) in a Zoo environment. The aim of the study was to determine the body feature most suitable for recording a koala’s body temperature (using coefficient of variation scores). We used a FLIR530TM IR thermal imaging camera to take images of each individual koala across three days in autumn 2018 at the Wildlife Sydney Zoo, Australia. Our results demonstrated that koalas had more than one reliable body feature for recording body temperature using the thermal imaging tool—the most reliable features were eyes and abdomen. This study provides first reported application of thermal imaging on an Australian native species in a Zoo and demonstrates its potential applicability as a humane/non-invasive technique for assessing the body temperature as an index of stress.


2020 ◽  
Author(s):  
Malika Nisal Ratnayake ◽  
Adrian G Dyer ◽  
Alan Dorin

AbstractMonitoring animals in their natural habitat is essential for advancement of animal behavioural studies, especially in pollination studies. Non-invasive techniques are preferred for these purposes as they reduce opportunities for research apparatus to interfere with behaviour. One potentially valuable approach is image-based tracking. However, the complexity of tracking unmarked wild animals using video is challenging in uncontrolled outdoor environments. Out-of-the-box algorithms currently present several problems in this context that can compromise accuracy, especially in cases of occlusion in a 3D environment. To address the issue, we present a novel hybrid detection and tracking algorithm to monitor unmarked insects outdoors. Our software can detect an insect, identify when a tracked insect becomes occluded from view and when it re-emerges, determine when an insect exits the camera field of view, and our software assembles a series of insect locations into a coherent trajectory. The insect detecting component of the software uses background subtraction and deep learning-based detection together to accurately and efficiently locate the insect among a cluster of wildflowers.We applied our method to track honeybees foraging outdoors using a new dataset that includes complex background detail, wind-blown foliage, and insects moving into and out of occlusion beneath leaves and among three-dimensional plant structures. We evaluated our software against human observations and previous techniques. It tracked honeybees at a rate of 86.6% on our dataset, 43% higher than the computationally more expensive, standalone deep learning model YOLOv2. We illustrate the value of our approach to quantify fine-scale foraging of honeybees. The ability to track unmarked insect pollinators in this way will help researchers better understand pollination ecology. The increased efficiency of our hybrid approach paves the way for the application of deep learning-based techniques to animal tracking in real-time using low-powered devices suitable for continuous monitoring.


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