scholarly journals Organic N-fertilizer, rhizobacterial inoculation and fungal compost improve nutrient uptake, plant growth and the levels of vindoline, ajmalicine, vinblastine, catharanthine and total alkaloids in Catharanthus roseus L.

2018 ◽  
Vol 30 (2) ◽  
pp. 203-213 ◽  
Author(s):  
Davood Hashemabadi ◽  
Fatemeh Sabzevari ◽  
Behzad Kaviani ◽  
Mohammad Hossein Ansari
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Nutrient Uptake ◽  
Catharanthus Roseus ◽  
High Performance ◽  
N Fertilizer ◽  
Organic N ◽  
Mineral Fertilizers ◽  
Total Alkaloids ◽  
Biological Treatments

Abstract The aim of the study was to replace mineral fertilizers with organic and biological fertilizers to improve nutrient uptake, plant growth and the concentrations of some important secondary metabolites in periwinkle (Catharanthus roseus L.). Periwinkle plants were grown under different rates of N supply (0, 20 and 40 mg kg−1 soil) and biological treatments (Azospirillum, Azotobacter, Azospirillum plus Azotobacter, Azospirillum plus fungal compost, Azotobacter plus fungal compost, and fungal compost). The concentrations of pigments and nutrients were measured by spectrophotometry and flame photometry. Secondary metabolites were analyzed by high-performance liquid chromatography (HPLC). Data were recorded for plant growth and development parameters, nutrient uptake and some secondary metabolites of periwinkle plants. The results showed that the N-fertilizer and biological treatments significantly improved most growth attributes and nutrient uptake and increased the concentrations of secondary metabolites as compared to the control. Maximum concentrations of root ajmalicine (0.54 mg g−1 DW), leaf vinblastine (0.96 mg g−1 DW) and root catharanthine (2.38 mg g−1 DW) were obtained from the treatment with Azospirillum under N-fertilizer at 20 and 40 mg kg−1 soil. Azotobacter along with fungal compost under N-fertilizer at 40 mg kg−1 soil induced the maximum concentration of leaf vindoline (1.94 mg g−1 DW). The highest concentration of root alkaloids (1.11 mg g−1 DW) was obtained from the treatment with compost under 40 mg N kg−1 soil. Azospirillum, Azotobacter and fungal compost combined with the N-fertilizer improved many morphological and nutrient characteristics. In conclusion, the growth and metabolism of C. roseus were significantly positively affected by the organic and biological fertilizers.

scholarly journals Antimicrobial activities of secondary metabolites of endophytic fungi isolated from Catharanthus roseus

2017 ◽  
Vol 7 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Mark O. Akpotu ◽  
Peter M. Eze ◽  
Chika C. Abba ◽  
Blessing O. Umeokoli ◽  
Charles U. Nwachukwu ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Catharanthus Roseus ◽  
High Performance ◽  
Antimicrobial Activities ◽  
Antibacterial And Antifungal Activities ◽  
Rice Medium ◽  
Antibacterial And Antifungal ◽  
Promising Source

Introduction: Recently, several endophytes have been shown to possess the potentials to synthesize novel bioactive compounds that have found use for drug discovery. We isolated endophytic fungi associated with Catharanthus roseus collected from the river banks of Amassoma in Southern Nigeria, and identified some of their bioactive secondary metabolites. Methods: The fungi were subjected to solid-state fermentation on rice medium and the metabolites were extracted using ethyl acetate. The fungal crude extracts were screened for antimicrobial activity and were also subjected to high-performance liquid chromatography-diode-array detection (HPLC-DAD) analysis for the identification of the bioactive compounds. Results: The fungal extracts showed both antibacterial and antifungal activities with minimum inhibitory concentrations ranging from 0.0625 to 1 mg/mL. The HPLC-DAD analysis of the extracts suggested the presence of citreoisocoumarin, citreoisocoumarinol, questinol, hydroxyemodin, acropyrone, methyl 2-(4-hydroxyphenyl) acetate, nigricinol, and cladosporin. Conclusion: The results of this study suggest that endophytic fungi associated with C. roseus could be a promising source of novel bioactive compounds with pharmaceutical and industrial importance.

scholarly journals PGPR Modulation of Secondary Metabolites in Tomato Infested with Spodoptera litura

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 778 ◽  
Author(s):  
Bani Kousar ◽  
Asghari Bano ◽  
Naeem Khan
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Spodoptera Litura ◽  
High Performance ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth And Yield ◽  
Infected Leaf ◽  
Tomato Plants ◽  
Plant Growth Promoting ◽  
Growth Promoting

The preceding climate change demonstrates overwintering of pathogens that lead to increased incidence of insects and pest attack. Integration of ecological and physiological/molecular approaches are imperative to encounter pathogen attack in order to enhance crop yield. The present study aimed to evaluate the effects of two plant growth promoting rhizobacteria (Bacillus endophyticus and Pseudomonas aeruginosa) on the plant physiology and production of the secondary metabolites in tomato plants infested with Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). The surface sterilized seeds of tomato were inoculated with plant growth promoting rhizobacteria (PGPR) for 3–4 h prior to sowing. Tomato leaves at 6 to 7 branching stage were infested with S. litura at the larval stage of 2nd instar. Identification of secondary metabolites and phytohormones were made from tomato leaves using thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) and fourier-transform infrared spectroscopy (FTIR). Infestation with S. litura significantly decreased plant growth and yield. The PGPR inoculations alleviated the adverse effects of insect infestation on plant growth and fruit yield. An increased level of protein, proline and sugar contents and enhanced activity of superoxide dismutase (SOD) was noticed in infected tomato plants associated with PGPR. Moreover, p-kaempferol, rutin, caffeic acid, p-coumaric acid and flavonoid glycoside were also detected in PGPR inoculated infested plants. The FTIR spectra of the infected leaf samples pre-treated with PGPR revealed the presence of aldehyde. Additionally, significant amounts of indole-3-acetic acid (IAA), salicylic acid (SA) and abscisic acid (ABA) were detected in the leaf samples. From the present results, we conclude that PGPR can promote growth and yield of tomatoes under attack and help the host plant to combat infestation via modulation in IAA, SA, ABA and other secondary metabolites.

scholarly journals Soil Fertility Status, Nutrient Uptake and Productivity of Marigold (Tagetes erecta Linn.) as Influenced by Fertilizer Doses and Plant Growth Regulators

2020 ◽  
pp. 53-65
Author(s):  
Nilay Borah ◽  
J. Deka ◽  
N. C. Deka ◽  
I. C. Barua ◽  
B. K. Saud ◽  
...  
Keyword(s):  
Plant Growth ◽  
Soil Fertility ◽  
Field Experiment ◽  
Nutrient Uptake ◽  
Growth Regulators ◽  
Foliar Spray ◽  
Mineral Fertilizers ◽  
Tagetes Erecta ◽  
Fertility Status ◽  
Soil Fertility Status

Aims: A field experiment was conducted to evaluate soil fertility status, nutrient uptake, growth and yield of African marigold (Tagetes erecta Linn.) as influenced by fertilizer management with or without foliar spray of plant growth promoting chemicals. Study Design: The field experiment was conducted with eight treatments, each being replicated thrice in a randomized block design (RBD). Place and Duration of Study: Instructional cum research (ICR) farm, Assam Agricultural University, Jorhat, Assam (India), between September 2013 and January 2015. Methodology: The treatments comprised of an unfertilized plot, application of 2500 kg ha-1 vermicompost at planting followed by 10 kg ha-1 each of N, P2O5 and K2O in two equal splits at the time of planting and at 30 days after planting (DAP), 2500 kg ha-1 vermicompost as mixture with fertilizer (comlizer) 10:10:10 kg ha-1 (Comlizer-1) or 30:10:30 kg ha-1 (Comlizer-2) of N, P2O5 and K2O, respectively applied in two splits (planting and at 30 DAP). Each comlizer treatment had two supplementary treatments of spraying either vermiwash solution (10% on second and third weeks and 20% solution on fourth and fifth weeks after planting) or indole butyric acid (IBA) and kinetin mixture (IBA-kinetin) solution (0.01% each on second and third weeks and 0.02% each on fourth and fifth weeks after planting), respectively. Results: The soil pH, organic carbon and available phosphorous contents after harvest of the crop were not affected by the treatments. The available nitrogen content in soil significantly increased in comlizer-2, but the available potassium content was not affected by the fertilizer doses. The NH4-N and NO3-N in soil at 15 and 45 DAP increased significantly with application of comlizer-2. Phosphorous and potassium uptake by aerial biomass of marigold at 21 and 42 days after planting significantly increased in Comlizer-2, but the effect of foliar spray was not significant. The highest fresh flower yield was produced by application of Comlizer-2 with foliar spray of vermiwash, and differed significantly to the rest of the treatments. Conclusion: Application of mineral fertilizers as mixture with vermicompost and integrated with foliar spray of growth regulators showed promising results. Further works with higher doses of nutrient and compost, frequency and intensity of foliar application of growth regulators are required to develop cost effective nutrient management practice.

scholarly journals Metal Accumulation Profile of Catharanthus roseus (L.) G.Don and Celosia argentea L. with EDTA Co-Application

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 598
Author(s):  
Muneeba Qurban ◽  
Cyrus Raza Mirza ◽  
Aqib Hassan Ali Khan ◽  
Walid Khalifa ◽  
Mustapha Boukendakdji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Catharanthus Roseus ◽  
Metal Uptake ◽  
Metal Accumulation ◽  
Metal Tolerance ◽  
Plant Biomass ◽  
Ethylenediaminetetraacetic Acid ◽  
Ornamental Plants ◽  
Environmental Deterioration ◽  
Celosia Argentea

The problem of metal-induced toxicity is proliferating with an increase in industrialization and urbanization. The buildup of metals results in severe environmental deterioration and harmful impacts on plant growth. In this study, we investigated the potential of two ornamental plants, Catharanthus roseus (L.) G.Don and Celosia argentea L., to tolerate and accumulate Ni, Cr, Cd, Pb, and Cu. These ornamental plants were grown in Hoagland’s nutrient solution containing metal loads (50 µM and 100 µM) alone and in combination with a synthetic chelator, ethylenediaminetetraacetic acid (EDTA) (2.5 mM). Plant growth and metal tolerance varied in both plant species for Ni, Cr, Cd, Pb, and Cu. C. roseus growth was better in treatments without EDTA, particularly in Ni, Cr, and Pb treatments, and Pb content increased in all parts of the plant. In contrast, Cd content decreased with EDTA addition. In C. argentea, the addition of EDTA resulted in improved plant biomass at both doses of Cu. In contrast, plant biomass reduced significantly in the case of Ni. In C. argentea, without EDTA, root length in Cd and Cu treatments was significantly lower than the control and other treatments. However, the addition of EDTA resulted in improved growth at both doses for Pb and Cu. Metal accumulation in C. argentea enhanced significantly with EDTA addition at both doses of Cu and Cd. Hence, it can be concluded that EDTA addition resulted in improved growth and better metal uptake than treatments without EDTA. Metal accumulation increased with EDTA addition compared to treatments without EDTA, particularly for Pb in C. roseus and Cu and Cd in C. argentea. Based on the present results, C. roseus showed a better ability to phytostabilize Cu, Cd, and Ni, while C. argentea worked better for Ni, Cd, Cu, and Pb.

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