scholarly journals The Untapped Potential Of Plant Thin Cell Layers

2015 ◽  
Vol 23 (2) ◽  
pp. 127-131 ◽  
Author(s):  
Jaime Teixeira da Silva ◽  
Maria Maddalena Altamura ◽  
Judit Dobránszki
Keyword(s):  
Tissue Culture ◽  
Molecular Biology ◽  
Culture System ◽  
Historical Background ◽  
Thin Cell Layers ◽  
Commercial Importance ◽  
Dicotyledonous Plants ◽  
Cell Layers ◽  
Number Of Cells

Abstract Thin cell layers (TCLs), which contain a small number of cells or tissues, are explants excised from different organs (stems, leaves, roots, inflorescences, flowers, cotyledons, hypocotyls/epicotyls, and embryos). After almost 45 years of research, this culture system has been used for several monocotyledonous and dicotyledonous plants of commercial importance, and for model plants. The limited amount of cells in a TCL is of paramount importance because marker molecules/genes of differentiation can be easily localized in situ in the target/responsive cells. Thus, the use of TCLs has allowed, and continues to allow, for the expansion of knowledge in plant research in a practical and applied manner into the fields of tissue culture and micropropagation, cell and organ genetics, molecular biology, biochemistry, and development. Starting from a brief historical background, the actual and potential uses of the TCL system are briefly reviewed.

scholarly journals VARIATION FOR GENETIC RECOMBINATION AMONG TOMATO PLANTS REGENERATED FROM THREE TISSUE CULTURE SYSTEMS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1164e-1164 ◽  
Author(s):  
Michael E. Compton ◽  
Richard E. Veilleux
Keyword(s):  
Tissue Culture ◽  
Genetic Recombination ◽  
Shoot Tips ◽  
Chromosome 3 ◽  
Chromosome 2 ◽  
Recombination Rates ◽  
Culture Systems ◽  
Thin Cell Layers ◽  
Cell Layers ◽  
Map Distance

Genetic recombination rates of hybrid plants regenerated from three tissue culture. systems were compared by backcrossing regenerated plants with mutant parents and comparing the observed crossover frequencies with those expected based on control plants raised from seed. Increased recombination rates and map distances were observed among plants from micropropagated shoot tips (4.5%-5.9%), cotyledon calli (3.7%-8.5%), and thin cell layers (2.8%-6.5%) between the sunny (sy) and baby leaf syndrome (bls) markers which flank the centromere on chromosome 3. Conversely, a decrease in map distance was observed between bls and the solanifolia (sf) locus which is more distal to the centromere on the same arm of chromosome 3 as bls. Increased map distance among plants regenerated from micropropagated shoot tips, cotyledon calli, and thin cell layers was also observed between white virescence (wv) and anthocyanin reduced (are) loci on chromosome 2.

High Density Tissue Culture on Microporous Membranes Perfused by Blood: Report of a New Bioartificial System

1978 ◽  
Vol 1 (6) ◽  
pp. 280-287 ◽  
Author(s):  
D.C. White ◽  
T. Kolobow ◽  
R.L. Bowman
Keyword(s):  
Tissue Culture ◽  
Culture System ◽  
High Density ◽  
Fetal Sheep ◽  
Arterial Blood ◽  
Density Growth ◽  
Polycarbonate Membranes ◽  
High Density Cell ◽  
Sheep Thymus

A new tissue culture system has been developed to sup-port high density cell growth using the sheep as the host. Cellular nutrition is provided by plasma solutes as they diffuse from arterial blood across microporous polycarbonate membranes into a device attached to an arteriovenous shunt. Culture chambers are constructed with transparent polycarbonate to allow photomicroscopy of the tissue in situ. System performance is demonstrated by the high density growth of fetal sheep thymus allografts and rat soft tissue sarcoma xenografts.

Free and conjugated polyamines during de novo floral and vegetative bud formation in thin cell layers of tobacco

1987 ◽  
Vol 70 (3) ◽  
pp. 453-460 ◽  
Author(s):  
Patrizia Torrigiani ◽  
Maria Maddalena Altamura ◽  
Gabriella Pasqua ◽  
Barbara Monacelli ◽  
Donatella Serafini-Fracassini ◽  
...  
Keyword(s):  
De Novo ◽  
Bud Formation ◽  
Thin Cell Layers ◽  
Conjugated Polyamines ◽  
Cell Layers ◽  
Free And Conjugated Polyamines ◽  
Vegetative Bud

scholarly journals Molecular biology approaches in bioadhesion research

2014 ◽  
Vol 5 ◽  
pp. 983-993 ◽  
Author(s):  
Marcelo Rodrigues ◽  
Birgit Lengerer ◽  
Thomas Ostermann ◽  
Peter Ladurner
Keyword(s):  
Molecular Biology ◽  
Biological Function ◽  
Molecular Approach ◽  
Research Groups ◽  
Blast Search ◽  
Search Facility ◽  
New Research ◽  
And Function ◽  
Functional Analyses

The use of molecular biology tools in the field of bioadhesion is still in its infancy. For new research groups who are considering taking a molecular approach, the techniques presented here are essential to unravelling the sequence of a gene, its expression and its biological function. Here we provide an outline for addressing adhesion-related genes in diverse organisms. We show how to gradually narrow down the number of candidate transcripts that are involved in adhesion by (1) generating a transcriptome and a differentially expressed cDNA list enriched for adhesion-related transcripts, (2) setting up a BLAST search facility, (3) perform an in situ hybridization screen, and (4) functional analyses of selected genes by using RNA interference knock-down. Furthermore, latest developments in genome-editing are presented as new tools to study gene function. By using this iterative multi-technologies approach, the identification, isolation, expression and function of adhesion-related genes can be studied in most organisms. These tools will improve our understanding of the diversity of molecules used for adhesion in different organisms and these findings will help to develop innovative bio-inspired adhesives.

Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus

1999 ◽  
Vol 18 (11) ◽  
pp. 935-940 ◽  
Author(s):  
F. Carimi ◽  
F. De Pasquale ◽  
F. G. Crescimanno
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Thin Cell Layers ◽  
Cell Layers

Cultured epithelial cells derived from human foetal pancreas as a model for the study of cystic fibrosis: further analyses on the origins and nature of the cell types

1988 ◽  
Vol 90 (1) ◽  
pp. 73-77
Author(s):  
A. Harris ◽  
L. Coleman
Keyword(s):  
Cystic Fibrosis ◽  
Tissue Culture ◽  
Epithelial Cells ◽  
Culture System ◽  
Cultured Cells ◽  
Cell Types ◽  
Cultured Epithelial Cells ◽  
Different Cell Types ◽  
Foetal Pancreas

The establishment of a tissue-culture system for epithelial cells derived from human foetal pancreas has recently been reported. Further analyses have now been made on these cells in vitro, together with parallel investigation of the distribution of different cell types within the intact foetal pancreas. Results support the view that the cultured cells are ductal in origin and nature. Pancreatic epithelial cell cultures have also been established from foetuses with cystic fibrosis.

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