The morphology of an unique population of Corynosoma strumosum (Acanthocephala, Polymorphidae) from the Caspian seal, Pusa caspica, in the land-locked Caspian Sea using SEM, with special notes on histopathology

2011 ◽  
Vol 56 (4) ◽  
Author(s):  
Omar Amin ◽  
Richard Heckmann ◽  
Ali Halajian ◽  
Atif El-Naggar
Keyword(s):  
Light Microscopy ◽  
Caspian Sea ◽  
Young Male ◽  
Host Tissue ◽  
Isolated Population ◽  
Sem Images ◽  
Intestinal Villi ◽  
Proboscis Hook ◽  
First Time ◽  
Caspian Seal

AbstractOver 700 specimens of Corynosoma strumosum (Rudolphi, 1802) Lühe, 1904 were collected from one young male Caspian seal, Pusa caspica (Gmelin) in the southern land-locked Caspian Sea in April, 2009. Collected worms showed consistent variations from those reported by other observers using light microscopy especially in proboscis hook and trunk spine patterns. SEM images revealed many features that have not been previously reported including the shape and distribution of trunk spines, dorsoventral differences in proboscis hooks and their organization, the baldness of anterior proboscis, the rough egg topography, epidermal micropores, and variations in the female gonopore. This isolated population of C. strumosum from the land-locked Caspian Sea is distinguished from others reported from open waters elsewhere by the distribution of trunk spines, consistently smaller size of trunk and testes, larger eggs, and fewer proboscis hooks. Histopathological sections reveal the invasive path of worms in host tissue with damage to intestinal villi and worm encapsulation. Information obtained from SEM studies and histopathological sections is reported for the first time.

Petalomonas sphagnophila (Euglenophyta, Petalomonadales), a new euglenophyte species for Russia

2016 ◽  
Vol 50 ◽  
pp. 112-119
Author(s):  
D. A. Kapustin ◽  
D. A. Philippov ◽  
I. V. Sokolova ◽  
E. S. Gusev
Keyword(s):  
Light Microscopy ◽  
Original Publication ◽  
Raised Bog ◽  
Vologda Region ◽  
First Time

Petalomonas sphagnophila has been found for the first time in Russia from the Shichengskoe raised bog (Vologda Region). Morphology of the species is studied by means of light microscopy and illustrated by original micrographs. The data on its taxonomy, ecology and distribution are given. The name P. sphagnophila was not validly published because no type was indicated in the original publication (Christen, 1962), so we are validating the name by indicating its holotype. P. polytaphrena Skuja, nom. inval. is treated as a synonym of P. sphagnophila. Currently, thirty species of the genus Petalomonas, including P. sphagnophila, are known from Russia.

scholarly journals Petal and Sepal Epidermal Micromorphology of Six Lathyrus Taxa (Fabaceae) and their Systematic Value

2012 ◽  
Vol 40 (1) ◽  
pp. 35 ◽  
Author(s):  
Hüseyin CILDIR ◽  
Ahmet KAHRAMAN ◽  
Musa DOGAN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Glandular Trichomes ◽  
Systematic Significance ◽  
Trichome Types ◽  
First Time ◽  
Scanning Electron

In this study, the epidermal types and their distribution on dorsal and lateral petals, the trichome types and their density on sepals of Lathyrus chloranthus, L. digitatus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, L. sativus, and L. tuberosus, belonging to sections Lathyrus, Lathyrostylis, Pratensis, and Orobon of the genus Lathyrus in Turkey were investigated using light microscopy (LM) and scanning electron microscopy (SEM), and the systematic significance of these characters was evaluated. These taxa, except L. sativus, are studied for the first time under aspects of the petal and sepal micromorphology. Three major epidermal types were recognized on the petal: tabular rugose striate cells (TRS), areolate cells with more or less striations (AS), and papillose conical striate cells (PCS). TRS and AS were further subdivided into three subtypes. TRS was found on the dorsal and lateral petals of L. chloranthus, L. sativus, and L. tuberosus: the dorsal petals of L. roseus subsp. roseus and the lateral petals of L. laxiflorus subsp. laxiflorus, while AS was present on the dorsal and lateral petals of L. digitatus and the lateral petals of L. roseus subsp. roseus. PCS was found only on the dorsal petals of L. digitatus. Three main types of trichomes on the sepal were observed: peltate glandular, capitate glandular, and nonglandular trichomes. The capitate glandular and nonglandular trichomes were further subdivided into three subtypes. The peltate glandular trichomes were present only in L. chloranthus, but absent in the others. The capitate glandular trichomes were found in L. chloranthus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, and L. tuberosus. The nonglandular trichomes were always present in L. chloranthus and L. laxiflorus subsp. laxiflorus. The present results show that the petal and sepal micromorphology can be used in delimitation of the taxa based on petal and sepal micromorphology.

scholarly journals Preloading Effect On The Strength of Cement Mass Mixing Treated Salty Sand

2021 ◽  
Author(s):  
Rasoul Alipour
Keyword(s):  
Thick Layer ◽  
Test Results ◽  
Cement Slurry ◽  
Sem Images ◽  
Treated Soil ◽  
Pure Salt ◽  
Ucs Test ◽  
Southwest Of Iran ◽  
First Time ◽  
Electronic Microscope

Abstract Existing problematic sub-layers in mixing technologies are a challenge, and for the first time, the effects of salt sub-layers in mass mixing technology have been investigated in this study for sandy salt in the southwest of Iran. This paper discusses the influence of adding various cement contents, Aw, and imposing different preloading values on the salty sand soil. First, salt and sand samples were dried, then, 90 % sand was mixed with 10% salt. After that, 30 % water was mixed thoroughly with the mixture of salty sand to obtain slurries. Cement slurry at a water-cement ratio (w/c) of 0.6 was then added to the sample and thoroughly mixed. The amount of cement in a slurry form that was added to the salty soil was 2, 4, 6, 8, and 10% by mass of dry soil. Each treated soil preloaded by 0, 9, and 45 kPa. After 120 days, the unconfined compressive strength of the sample was determined. Furthermore, by Scanning Electronic Microscope, SEM, the microstructures of treated samples were analyzed. At the end Unconfined Compression Strength, UCS, test results normalized to the non-preloaded treated soil. By increasing cement content, the effect of preloading in increasing UCS will decrease. In the SEM images for Aw=2%, the effect of preloading indicates porous shape for non-preloaded samples. Vice versa by Aw=8%, porous shape in the SEM images will disappear. In the end, treatability studies of pure salt in the thick layer have been investigated.

scholarly journals A new species of Litarachna Walter, 1925 (Acari: Hydrachnidia: Pontarachnidae) from Corozal Bay (Belize), described based upon morphology and DNA barcodes

Acarologia ◽  
2021 ◽  
Vol 61 (3) ◽  
pp. 602-613
Author(s):  
Lucia Montes-Ortiz ◽  
Tom Goldschmidt ◽  
Lourdes Vásquez-Yeomans ◽  
Manuel Elías-Gutiérrez
Keyword(s):  
Dna Barcode ◽  
Morphological Description ◽  
Estuarine System ◽  
Dna Barcodes ◽  
Sem Images ◽  
The Family ◽  
Coi Sequences ◽  
First Time ◽  
A New Species ◽  
Electronic Microscope

A new planktonic species of the marine water mites of the family Pontarachnidae Koenike, 1910 is described from Corozal Bay, an estuarine system in Belize. The morphological description includes Scanning Electronic Microscope (SEM) images and is augmented by an analysis of DNA cytochrome c oxidase I (COI) sequences, the DNA barcode, used for the first time for a species description in this group.

scholarly journals Pollen morphology of the Balkan-Carpathian endemic Campanula lingulata Waldst. & Kit. (Campanulaceae)

2016 ◽  
pp. 75-82
Author(s):  
Marina Macukanovic-Jocic ◽  
Snezana Jaric
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Endemic Species ◽  
Pollen Morphology ◽  
Average Length ◽  
Pollen Grains ◽  
Polar Axis ◽  
First Time ◽  
Scanning Electron

Palynomorphological characteristics of Campanula lingulata, the Balkan-Carpathian endemic species growing in Serbia, have been investigated using light microscopy and scanning electron microscopy for the first time, in order to provide some information helpful for a better understanding of the taxonomic position of this species within the genus, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains are radially symmetrical, isopolar, 3-zonoporate and medium-sized monads oblate-sphaeroidal in shape. Mean of the polar axis (P) is 27.6?1.9 ?m, while the average length of the equatorial axis (E) is 28.8?1.6 ?m. The apertures are operculate. The sculpturing pattern of the exine is microre?ticulate-microechinatae. The exine surface is covered with evenly distributed supratectal spinules of variable length and sparse granules. The longest supratectal spinules are 0.64?0.05 ?m in length and the smallest sculptural elements are less than 0.2 ?m high. The microechinae density per sample area of 5 ?m x 5 ?m averages 17.4?2.4.

Micromorphological studies of leaf epidermal features in populations of maples (Acer L.) from Iran

Phytotaxa ◽  
2017 ◽  
Vol 299 (1) ◽  
pp. 36
Author(s):  
MOJGANSADAT MOHTASHAMIAN ◽  
FARIDEH ATTAR ◽  
KAVEH KAVOUSI ◽  
ALI MASOUDI-NEJAD
Keyword(s):  
Native Species ◽  
Deciduous Trees ◽  
Epicuticular Waxes ◽  
Abaxial Surface ◽  
Sem Images ◽  
Qualitative Variables ◽  
Epidermal Features ◽  
Parametric Analyses ◽  
First Time

As the largest genus of broad leaved deciduous trees, Acer L. contains about 126 species distributed in the temperate regions of the Northern Hemisphere. With eight native species in Iran, maples are among the most important tree species in the country. Micromorphological traits of 39 populations of Iranian native species indicated the value of leaf epidermal characteristics in identification and classification of maples. A number of epidermal morphological features of the abaxial surface were investigated using LM and SEM images. The occurrence of stomata in clusters is reported for the first time in Acer. The shape of the epidermal cells, anticlinal cell wall patterns, type of indumentum, and epicuticular waxes were the most significant variables in this study. The ability of quantitative and qualitative variables in segregating the studied taxa was evaluated by statistical methods, including PCA, MCA and Non-parametric analyses.

Patterns of Abnormal Myogenesis in Human Cleft Palates

1994 ◽  
Vol 31 (5) ◽  
pp. 345-350 ◽  
Author(s):  
Steven R. Cohen ◽  
Lynn L. Chen ◽  
Alphonse R. Burdi ◽  
Carroll-Ann Trotman
Keyword(s):  
Light Microscopy ◽  
Cleft Palate ◽  
Muscle Fiber ◽  
Soft Palate ◽  
Crown Rump Length ◽  
Levator Veli Palatini ◽  
Myotube Formation ◽  
Preliminary Study ◽  
Mesenchymal Condensation ◽  
First Time

To test the hypothesis that soft palate muscles are abnormal in cleft palate, we compared soft palate morphogenesis in fetuses with cleft palate (n=4) to age-matched (n=3) and nonmatched (n=1) control specimens. The morphologic status of all soft palate and masticatory structures were classified into one of six stages based on the level of histogenesis. At 54 mm crown-rump length (CRL), the levator veli palatini (L), palatopharyngeus (PP), and palatoglossus (PG) in cleft subjects demonstrated mesenchymal condensation into myoblastic fields, lagging behind the control specimens (97 mm CRL), which displayed definitive fields of myoblasts and myotube formation. In the 175 mm and 225 mm cleft and the 170 mm and 192 mm control specimens, muscular morphology was similar and had reached its postnatal appearance for the tensor veli palatini (175 m only) and L, PP, PG (225 mm only). Muscle fiber directions were, however, disoriented and disorganized, especially close to the medial epithelial edge of the cleft. The levator veli palatini, could not be distinguished as a discrete muscle in the cleft specimens, and what we believed to be the PP and PG seemed “normal” at the level of light microscopy, but malpositioned in a superior direction. This preliminary study demonstrates for the first time that early myogenesis in cleft palates differs from normal.

First Multistage Hydraulic Fracturing in Russian Offshore: Integrated Approach to Completion and Stimulation of Apt Deposits of V.Filanovsky Field

2021 ◽  
Author(s):  
Mikhail Yurievich Golenkin ◽  
Denis Vladimirovich Eliseev ◽  
Alexander Anatolyevich Zemchikhin ◽  
Alexey Alexandrovich Borisenko ◽  
Akhmat Sakhadinovich Atabiyev ◽  
...  
Keyword(s):  
Hydraulic Fracturing ◽  
Caspian Sea ◽  
Complex Type ◽  
The Caspian Sea ◽  
Multistage Hydraulic Fracturing ◽  
Coiled Tubing ◽  
Sand Control ◽  
Friction Reducing ◽  
First Time ◽  
Stimulation Of

Abstract The paper describes the results of the first multistage hydraulic fracturing operations in Russia on the Caspian Sea shelf in the gas condensate and oil deposits of the Aptian formation of V. Filanovsky field. In addition to the small productive formation depth, long horizontal sections with a complex trajectory and high collapse gradients due to large zenith angles when passing the Albian and Aptian deposits of poorly consolidated sandstones are an additional challenge for choosing a multistage hydraulic fracturing assembly. The above features require the use of modern sand control screens with enhanced frac sleeves. A design was developed which includes frac sleeves and sand control screens that can withstand multiple cycles of hydraulic impact during hydraulic fracturing, as well as many opening/closing cycles. A seawater-based frac fluid system was applied. The frac fleet was located on a pontoon, the coiled tubing – on a platform. For the first time in Russia, a 2-5/8 inch coiled tubing with a complex-type friction reducing system was used to switch coupling/sleeves in conditions of very long horizontal sections, complex trajectories, and high friction coefficients. The minimum distances between the screen's sliding sleeves and frac sleeves did not prevent from performing manipulations in complex environment. For well cleaning, the frac assemblies of reverse rotary-pulse and rotary-directional types were used. At the first stage of the project, the development of an optimal method of well completion was successfully implemented. Due to the close interaction of the operating company, service company, and science & engineering team of the operator, for the first time in Russia the design of downhole equipment with the use of advanced technologies of sand control screens, frac sleeves was presented. This solution has proved its effectiveness – the downhole equipment has retained its operational properties after a long period of well operation and further in the process of hydraulic fracturing. At the second stage of the project, 32 MSHF operations were performed at four wells. To reduce nonproductive time and operational risks, a satellite communication complex was additionally deployed on the pontoon to join the engineering centers of Astrakhan, Moscow, and Houston. After finishing the well development, the design indicators for formation fluid rates were achieved, which proved the effectiveness of the stimulation of the field's target objects – this opens great prospects for further development of low-permeability reservoirs at offshore sites in the Caspian Sea. The successful project implementation and the achievement of the design values of oil flow rates has expanded the possibilities of commercial operation of the low-permeable Aptian formation, complicated by the presence of a gas cap and underlying water. A solution was presented for working in extended horizontal well sections with 2-5/8 inch coiled tubing together with a complex-type mechanical friction reducing system. The economic effect was achieved when solving tasks of manipulating mechanical screen couplings and frac port sleeves without the involvement of downhole tractors. The use of new solutions in the completion assembly made it possible to eliminate additional sand ingress problems.

scholarly journals Performance of Laser Fluorescence for Residual Caries Detection in Primary Teeth

2008 ◽  
Vol 02 (03) ◽  
pp. 176-184 ◽  
Author(s):  
Taskin Gurbuz ◽  
Yucel Yilmaz ◽  
Fatih Sengul
Keyword(s):  
Light Microscopy ◽  
Gold Standard ◽  
Primary Teeth ◽  
Polarized Light ◽  
Laser Fluorescence ◽  
Caries Detection ◽  
Sem Images ◽  
Significant Difference ◽  
Residual Caries ◽  
Cavity Floor

ABSTRACTObjectives: The aim of this study was to evaluate the performance of a visual-tactile examination and a laser fluorescence device for detecting residual dentinal caries after carious dentin removal with bur excavation, hand excavation and chemomechanical excavation (Carisolv™].Methods: Thirty extracted coronal caries primary second molars were used. The caries infected dentin has been removed. A blinded examiner checked all cavities for residual caries using a visualtactile examination and laser fluorescence. Then the teeth were sectioned through the prepared cavities andthe two halves of each tooth were processed for light microscopy and Scanning Electron Microscopy (SEM). The presence or absence of residual caries was verified using polarized light microscopy as the gold standard. The cavity floor dentin after removing carious dentin was examined using SEM.Results: In among all groups a significant difference is determined between visual-tactile examination and laser fluorescence (P<.05). There was an agreement between laser fluorescence or visual-tactile examination and histological gold standard (P>.05). In addition, SEM images of the surfaces with the caries removed have shown that a vast majority of the tubule openings is observed to be open in the samples of the Carisolv group in opposition to the other groups.Conclusions: The laser fluorescence system could be effective in checking the removal by other methods, such as bur or Carisolv and avoid excessive removal of the sound dentin. (Eur J Dent 2008;2:176-184)

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