scholarly journals PEMANFAATAN GESERAN POLA SPEKTRUM UV SENYAWA GOLONGAN BARBITURAT DALAM UJI KONFIRMASI DENGAN METODE TLC-SPEKTROFOTODENSITOMETRI

2019 ◽  
Vol 9 (1) ◽  
pp. 19
Author(s):  
I Nyoman Subadra ◽  
Luh Putu Mirah Kusuma Dewi Kusuma Dewi ◽  
I.N.K. Widjaja Widjaja
Keyword(s):  
Acid Solution ◽  
Stationary Phase ◽  
Ethyl Acetate ◽  
Alkali Solution ◽  
Uv Spectrum ◽  
Mobile Phases ◽  
Confirmation Test ◽  
Spectrum Shift

A Research of the utilization of spectrum shift in confirmation test for barbiturate using TLC-Spectrophotodensitometry has been done. Analyte was analyzed using two mobile phases, that are TD (chloroform: acetone = 80 : 20) and TE (ethyl acetate: methanol: ammonia = 85 : 15 : 5) with Al-TLC Silica 60 GF254 plate as the stationary phase. Afterwards, plate was sprayed with HCl or KOH. The analyzed parameters were hRfc, normal UV-spectrum pattern and its shift. The correlation of spectrum or the shift of UV-spectrum for barbiturate compounds was determined with Wincats program. The analysis of barbiturates which based on hRfc in error window (±7) for TD system and in error window (±11) for TE, still produced hit factor 8-25 compounds. The confirmation test for barbiturates based on hRfc and r ? 0,95, produced hit factor 1-2 compounds. Barbiturate compounds showed spectrum shift in alkali solution, while in acid solution did not happen. However, all four analytes contained identical spectrum shift, therefore the data of these spectrum shift can not be utilized for confirmation test of barbiturate compounds.

A Study on the variation of Apigenin content in Cardiospermum halicacabum from 21 districts of Tamil Nadu by HPTLC

2021 ◽  
pp. 2613-2618
Author(s):  
A Rajasekaran ◽  
V Abirami ◽  
S Arunkumar ◽  
Priya G Bharani ◽  
S Gugapriya ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Stationary Phase ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Tamil Nadu ◽  
Leaf Extract ◽  
Major Constituent ◽  
Marker Compound ◽  
Aluminium Sheets ◽  
Hptlc Method

Cardiospermum halicacabum is one of the most potent medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases, mainly for arthritis. Apigenin is one of the major constituent present in Cardiospermum halicacabum. The present study mainly aimed to estimate the content of major constituent apigenin present in Cardiospermum halicacabum collected from 21 districts of Tamil Nadu by HPTLC method using the marker compound apigenin. The HPTLC method was performed using HPTLC aluminium sheets precoated with Silica Gel 60 GF254 as stationary phase and Toluene: Ethyl acetate: formic acid: methanol (3:6:1.6:0.4 v/v) as the mobile phase. The developed chromatogram was scanned at 254nm using Camag Scanner III. The Rf value of standard apigenin and apigenin in the leaf extract of Cardiospermum halicacabum was found to be in the range of 0.80 to 0.89. Plant collected from Cuddalore district of Tamil Nadu was found to contain relatively high amount of marker compound apigenin than other regions.

scholarly journals PHENOL-2-(1-METHYL ETHOXY)-METHYL CARBAMATE COUMPOUND IN ETHYL ACETATE EXTRACT OF STEM BARK OF Aglaia angustifolia

2010 ◽  
Vol 8 (3) ◽  
pp. 463-468
Author(s):  
Sofnie M. Chairul
Keyword(s):  
Biological Activity ◽  
Stationary Phase ◽  
Ethyl Acetate ◽  
Polar Solvent ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Botanical Insecticide ◽  
Activity Test ◽  
Phase Mixture ◽  
Acetate Medium

Isolation of carbamate coumpound from ethyl acetate extract of stem bark of Aglaia angustifolia (Meliaceae), was carried out. The dried stem bark of A. angustifolia was extracted with ethanol (polar solvent), ethyl acetate (medium of polar) and water. From there extract solvent was biological activity test to Crocidolomia binotallis. Ethyl acetate extract solvent more active than another solvent, so that this extract was fractioned and clean up using chromatograpgy column, use SiO2 as stationary phase, mixture of n-hexane/ethyl acetate (10:1 ~ 1:1), ethyl acetate, and ethanol respectively as elution solution. The result of Biological activity test to C. binotallis showed that fraction of ethyl acetate inhibited growth on LC50 3.57 ppm. The compound of isolation result using HPLC, GCMS, FTIR and NMR was identified as phenol-2(1-methyl ethoxy) methyl carbamate coumpound, active as botanical insecticide.   Keywords: Meliaceae, A. angustifolia, carbamate, phenol-2 (1-methyl ethoxy) methyl carbamate

scholarly journals Normal and reversed phase thin-layer chromatography of new 16, 17-secoestrone derivatives

2003 ◽  
Vol 68 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Marijana Acanski ◽  
Suzana Jovanovic-Santa ◽  
Lidija Jevric
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Reversed Phase ◽  
Retention Behavior ◽  
Mobile Phases ◽  
Layer Chromatography

The retention behavior and separation ability of a series of new 16,17-secoestrone derivatives has been studied on silica gel, alumina and C-18 silica gel layers with non-aqueous and aqueous-organic mobile phases. The retention behavior and separation ability are discussed in terms of the nature of the solute, eluent and stationary phase.

Supercritical Adsorption

2005 ◽  
Author(s):  
Eldred H. Chimowitz
Keyword(s):  
Stationary Phase ◽  
Critical Point ◽  
Dynamic Models ◽  
Molecular Diffusion ◽  
Plug Flow ◽  
Fluid Phase ◽  
Commercial Application ◽  
Critical Systems ◽  
Mobile Phases ◽  
Column Dynamics

In this chapter, we discuss adsorption phenomena in supercritical systems, a situation that occurs in many application areas in chemical-process and materials engineering. An example of a commercial application in this area, which has achieved wide acceptance as a tool in analytical chemistry, is supercritical fluid chromatography (SFC). Not only is SFC a powerful technique for chemical analysis, but it also is a useful method for measuring transportive and thermodynamic properties in the near-critical systems. In the next section, we analyze adsorption-column dynamics using simple dynamic models, and describe how data from a chromatographic column can be used to estimate various thermodynamic and transport properties.We then proceed to discuss the effects of proximity to the critical point on adsorption behavior in these systems. The closer the system is to its critical point, the more interesting is its behavior. For very dilute solute systems, like those considered here, the energy balance is often ignored to a first approximation; this leads to a simple set of mass-balance equations defining transport for each species. These equations can be developed to various levels of complexity, depending upon the treatment of the adsorbent (stationary phase). The conceptual view of these phases can span a wide range of possibilities ranging from completely nonporous solids (fused structures) to porous materials with complicated ill-defined pore structures. Given these considerations, it is customary to make the following assumptions in the development of a simple model of adsorber-bed dynamics: . . .1. The stationary and mobile phases are continuous in the direction of the flow, with the fluid phase possessing a flat velocity profile (“plug” flow).. . . . . . 2. The porosity of the stationary phase is considered constant irrespective of pressure and temperature conditions (i.e., it is incompressible). . . . . . .3. The column is considered to be radially homogeneous, leading to a set of equations with one spatially independent variable, representing distance along the column axis. . . . . . . 4. The dispersion term in the model equation represents the combined effects of molecular diffusion and dispersion due to convective stirring in the bed. These effects are combined into an effective phenomenological dispersion coefficient, considered to be constant throughout the column. . . .

scholarly journals KAEMPFEROL FROM STELECHOCARPUS BURAHOL, (BL.) HOOK F. & TH. LEAVES AND XANTHINE OXIDASE INHIBITION ACTIVTY

2017 ◽  
Vol 10 (4) ◽  
pp. 322
Author(s):  
Diniatik K ◽  
Uwijiyo Pramono ◽  
Sugeng Riyanto
Keyword(s):  
Stationary Phase ◽  
Ethyl Acetate ◽  
Xanthine Oxidase ◽  
Aqueous Ethanol ◽  
Oxidase Inhibitor ◽  
Xanthine Oxidase Inhibitor ◽  
Ic50 Values ◽  
Oxidase Inhibition ◽  
Xanthine Oxidase Inhibition

Objective: Stelechocarpus burahol, (Bl.) Hook f. & Th. is a plant widely distributed in Java Island of Indonesia. The aim of this study is to identify compounds from the leaves of S. burahol that exhibited activity as xanthine oxidase inhibitor.Methods: The leaves were extracted with aqueous ethanol  and hidrolized with HCl methanol, then partitioned sequentially with chloroform and ethyl acetate. The ethyl acetate fractions were separated by coloumn chromatography with cellulose as stationary phase and methanol 50% as mobile phase.Results: Purification from this extracts afforded three compounds with one compound identified, namely Kaempferol. The four compounds possessed as xanthine oxidase inhibitor with IC50 values ranging from 0.27 to 0.45 μg/ml.Conclusion:Kaempferol exhibited the highest inhibition of 0.27 μg/ml.Keywords: Kaempferol, Xanthine Oxidase Inhibitor, Stelechocarpus burahol, (Bl.) Hook f. & Th. 

Detection of Tricresyl Phosphates and Determination of Tri-o-Cresyl Phosphate in Edible Oils

1985 ◽  
Vol 68 (6) ◽  
pp. 1074-1076
Author(s):  
Mahishi N Krishnamurthy ◽  
S Rajalakshmi ◽  
Om Prakash Kapur
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Stationary Phase ◽  
Ethyl Acetate ◽  
Edible Oils ◽  
Flame Ionization Detection ◽  
Tricresyl Phosphate ◽  
Flame Ionization ◽  
Layer Chromatography

Abstract Tricresyl phosphate (TCP) in contaminated edible oils was extracted using acetonitrile and detected by thin layer chromatography as well as gas chromatography (GC). The chromatoplate was developed with isooctane-ethyl acetate (90 + 10) and visualized by spraying with 2,6- dichloroquinone chloroimide. TCP gives a characteristic blue-violet spot when heated at 100°C for 15 min. The method is direct and sensitive and can be used to detect as low as 2.5 p.g TCP or TOCP (tri-o-cresyl phosphate). GC was carried out using 10% OV-101 as the stationary phase and flame ionization detection for confirmation and quantitation of TOCP in oils.

scholarly journals Separation and lipophilicity of some new steroid derivatives in normal- and reversed-phase high performance liquid chromatography

2011 ◽  
Vol 17 (4) ◽  
pp. 535-542 ◽  
Author(s):  
Marijana Acanski ◽  
Djura Vujuc ◽  
Suzana Jovanovic-Santa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Log P ◽  
Reversed Phase Hplc ◽  
Mobile Phases

The separation ability and retention of normal and reversed phase HPLC with, respectively, three non-polar and two polar mobile phases, have been studied by measuring the retention constants of a series of newly synthesized estrone derivatives. The separation ability and retention are discussed in terms of the nature of the solute, eluent and stationary phase. Good correlation was found between the retention constants log k0 of newly synthesized estrone derivatives obtained on C-18 column and log P calculated by different methods.

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