A Study on the variation of Apigenin content in Cardiospermum halicacabum from 21 districts of Tamil Nadu by HPTLC

Cardiospermum halicacabum is one of the most potent medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases, mainly for arthritis. Apigenin is one of the major constituent present in Cardiospermum halicacabum. The present study mainly aimed to estimate the content of major constituent apigenin present in Cardiospermum halicacabum collected from 21 districts of Tamil Nadu by HPTLC method using the marker compound apigenin. The HPTLC method was performed using HPTLC aluminium sheets precoated with Silica Gel 60 GF254 as stationary phase and Toluene: Ethyl acetate: formic acid: methanol (3:6:1.6:0.4 v/v) as the mobile phase. The developed chromatogram was scanned at 254nm using Camag Scanner III. The Rf value of standard apigenin and apigenin in the leaf extract of Cardiospermum halicacabum was found to be in the range of 0.80 to 0.89. Plant collected from Cuddalore district of Tamil Nadu was found to contain relatively high amount of marker compound apigenin than other regions.

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ANALYTICAL METHOD DEVELOPMENT AND ITS VALIDATION FOR EVALUATION OF POLYHERBAL FORMULATION

INDIAN DRUGS ◽

10.53879/id.55.10.10920 ◽

2018 ◽

Vol 55 (10) ◽

pp. 66-68

Author(s):

◽

A. P Jadhav

Keyword(s):

Immune Response ◽

Ethyl Acetate ◽

Analytical Method ◽

Mobile Phase ◽

Method Development ◽

Simultaneous Estimation ◽

Active Constituents ◽

Polyherbal Formulation ◽

Marker Compounds ◽

Hptlc Method

Talekt capsule is a branded polyherbal formulation used for enhancing the immune response to dermal infections and also for treating skin disorders. Curcumin and embelin, which are the active constituents of Haridra and Vidanga, respectively were used as marker compounds for developing a simple, accurate, precise and robust HPTLC method for simultaneous estimation. The mobile phase of toluene: ethyl acetate: formic acid (6.5: 3: 0.2 v/v/v) was used for separation. 381nm was used as wavelength for analysis. The Rf value obtained for curcumin, and embelin was found to be 0.51 ± 0.2 and 0.33 ± 0.2, respectively. The developed method was validated on the basis of ICH Q2 (R1) guidelines.

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Control of JE vector by organic compounds isolated and green nanoparticles synthesised from leaf extract of Holoptelea integrifolia (Roxb.)

10.21203/rs.2.20517/v1 ◽

2020 ◽

Author(s):

Someshwar Singha ◽

Goutam Chandra

Keyword(s):

Spectral Analysis ◽

Ethyl Acetate ◽

Larvicidal Activity ◽

Leaf Extract ◽

Ethyl Acetate Extract ◽

Good Alternative ◽

Nano Particles ◽

Major Constituent ◽

Active Principle ◽

Soxhlet Apparatus

Abstract Background: Japanese encephalitis (JE) is a dreadful disease transmitted by Culex vishnui group of mosquitoes. Control of JE vectors at the larval stage is one of the effective approaches in controlling JE. Methods: Leaves of Holoptelea integrifolia were subjected to petroleum ether, ethyl acetate, acetone and absolute alcohol solvent extraction by Soxhlet apparatus. As the ethyl acetate extract showed best mosquito larvicidal activity against Culex vishnui, it extract was selected and processed further for isolation of active principle through column chromatography and TLC and then characterization of the active principle by FTIR and GC-MS was done. Results: Ethyl acetate extract was found to be the most potent larvicide. In the active fraction of isolated compounds from ethyl acetate extract, N-methyl-1-adamantaneacetamide was the major constituent responsible for larvicidal activity of Culex vishnui. Green nanoparticles were synthesised by treating silver nitrate with leaf extract of H. integrifolia and were examined for larvicidal activity. Nanoparticles were characterised by UV-VIS spectral analysis, XRD study, TEM, SEM and FTIR spectral analysis. Synthesised nanoparticles were 40-50 nm in size and showed good larvicidal activity on Cx. vishnui mosquitoes at 1.25, 2.25, 5, 7.5 and 10 ppm concentrations. Active principle of plant extract and green nanoparticles showed eco-friendly effect on non-target organisms like Chironomus circumdatus, Daphnia sp, Diplonychus anulatum and Tadpole larvae. Conclusion: Thus it can be concluded that active ingredient as well as green synthesized nano particles from H. integrifolia can be a good alternative of presently used chemical insecticides.

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Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study

Antibiotics ◽

10.3390/antibiotics9060359 ◽

2020 ◽

Vol 9 (6) ◽

pp. 359 ◽

Cited By ~ 4

Author(s):

Prawez Alam ◽

Essam Ezzeldin ◽

Muzaffar Iqbal ◽

Gamal A.E. Mostafa ◽

Md. Khalid Anwer ◽

...

Keyword(s):

Ethyl Acetate ◽

Silica Gel ◽

Solid Lipid Nanoparticles ◽

Ternary Mixture ◽

Mobile Phase ◽

Pharmaceutical Formulations ◽

Lipid Nanoparticles ◽

Ammonia Solution ◽

Hptlc Method

In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using “RP-18 silica gel 60 F254S HPTLC plates”. However; NP-HPTLC estimation of DLFX was performed using “silica gel 60 F254S HPTLC plates”. For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 v/v/v) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 v/v/v) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at λmax = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX.

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STANDARDIZATION OF HYGROPHILA SPINOSA AND ITS FORMULATION WITH REFERENCE TO LUPEOL, BY DEVELOPED AND VALIDATED HPTLC AND RP-HPLC METHODS

INDIAN DRUGS ◽

10.53879/id.52.01.10182 ◽

2015 ◽

Vol 52 (01) ◽

pp. 13-19

Author(s):

R Jayaprakasam ◽

◽

M. F. Saleshier ◽

T. K. Ravi

Keyword(s):

Standard Deviation ◽

Ethyl Acetate ◽

Mobile Phase ◽

Solvent System ◽

Good Recovery ◽

Rp Hplc ◽

Relative Standard ◽

Optimum Wavelength ◽

Hptlc Method

The separation and determination of lupeol from Hygrophila spinosa were carried out by two simple, precise and accurate HPTLC and RP-HPLC methods. HPTLC method for the determination of lupeol from plant extract and its formulation was developed using a solvent system consisting of toluene : ethyl acetate : methanol (15: 3: 1.5%v/v/v). For detection, lupeol had to be derivatized with Liebermann Burchard reagent at 1050C. The optimum wavelength was fixed as 366nm. In RP-HPLC, the separation was carried out on a C18 column and the mobile phase selected was methanol: acetonitrile (30:70%v/v). The maximum wavelength was found to be 210nm.The method was validated in terms of various parameters. Low relative standard deviation and good % recovery values of both the methods showed that the developed methods were highly precise and accurate and therefore can be used for the standardization and quantification of lupeol in Hygrophila spinosa and its formulation.

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HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa

Chromatography Research International ◽

10.1155/2012/180103 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 5

Author(s):

Daya L. Chothani ◽

M. B. Patel ◽

S. H. Mishra

Keyword(s):

Ethyl Acetate ◽

Petroleum Ether ◽

Mobile Phase ◽

Ethyl Acetate Extract ◽

Marker Compound ◽

Biological Nature

The present study was aimed to identification, isolation, and quantification of marker in R. tuberosa (Acanthaceae). HPTLC fingerprinting was carried out for various extract of root, stem, and leaf of R. tuberosa. From the HPTLC fingerprint the florescent band (under 366 nm) at : 0.56 (mobile phase chloroform : toluene : ethyl acetate (6 : 3 : 1, v/v)) was found in leaf, root, and stem of R. tuberosa. So, the florescent band (under 366 nm) at : 0.56 was isolated as marker compound RT-F2 from root of R. tuberosa. The marker compound RT-F2 was quantified by using HPTLC technique. The percentage (W/W) amount of RT-F2 was found to 40.0% and 44.6% in petroleum ether and ethyl acetate extract of R. tuberosa roots, respectively. Further study is suggested to characterization and biological nature of marker compound.

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Molluscicidal Potential of Column Purified Fractions of Allium cepa against Intermediate Host of Urinary Schistosomiasis (Bulinus globosus) in Sokoto, Nigeria

Asian Journal of Research in Zoology ◽

10.9734/ajriz/2018/v1i129666 ◽

2018 ◽

pp. 1-6

Author(s):

J. Suleiman ◽

K. Singh ◽

A. Y. Bala ◽

M. T. Muhammad ◽

M. S. Yakubu

Keyword(s):

Stationary Phase ◽

Ethyl Acetate ◽

Intermediate Host ◽

Laboratory Condition ◽

Silica Gel ◽

Allium Cepa ◽

Mobile Phase ◽

Intermediate Hosts ◽

Urinary Schistosomiasis ◽

Bulinus Globosus

Potential of column purified fractions of Allium cepa bulb against intermediate hosts of urinary schistosomiasis (Bulinus globosus) was conducted in laboratory condition. The fresh bulbs of A. cepa were purchased from Ramin Kura market of Sokoto, identified and authenticated by a taxonomist. The bulbs were sliced into pieces, air dried and powdered. Extracts were obtained using methanol as polar then purified with silica gel as a stationary phase while N-hexane and ethyl acetate (1:1) as the mobile phase. Thirteen fractions each fraction containing 10 ml of the effluent was collected, the collected extracts were left open for evaporation for 48 hours. Ten adult B. globosus were immersed in 3liters of water containing different concentrations of the fraction and each treatment was replicated three times with control in the same condition without treatment, observations were recorded after 24 hours up to 96 hours. The toxicity experiment showed that fractions (F7, F8, F6 and F9) were most toxic fractions, LC50 after 96 hours was 19.371 mg/l. based on findings from this research it can be concluded that, A. Cepa was very potent and can be used for control of B. globosus in order to prevent urinary schistosomiasis infection in endemic areas and drugs industries may use the extracts of these plants for production of molluscicides.

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Development of bioanalytical standardization parameters of Alocasia indica tuber by HPTLC technique

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2020.120313 ◽

2020 ◽

pp. 295-299

Author(s):

Roman Kumar Aneshwari ◽

ANIL KUMAR SAHU ◽

Amber Vyas ◽

VISHAL JAIN

Keyword(s):

Formic Acid ◽

Ethyl Acetate ◽

Mobile Phase ◽

Ethanolic Extract ◽

Solvent System ◽

Perennial Herb ◽

Present Species ◽

Active Components ◽

Hptlc Method ◽

Routine Quality Control

Alocasia indica is perennial herb growing widely and used as traditional medicine in India, China and Bangladesh. The divine herb has potent medicinal values for the treatment of different type of illnesses. The HPTLC techniques were used to separate active components from ethanolic extract of tuber part of A. indica. This examination was intended to designed a HPTLC fingerprint profile of crude extract of the plant in ethanol. A HPTLC method for the isolation of various active constituents in A. indica ethanolic extract have been developed and solvent system for quercetin the mobile phase used was toluene: ethyl acetate: formic acid (5:2:1) and for analysis of β-sitosterol the mobile phase used was chloroform: ethyl acetate: formic acid (6:4:1) . In the present investigation, HPTLC fingerprint of extract of dried tuber part of A. indica have been performed and the results demonstrated that important information for standardization. The HPTLC system for routine quality control of present species can be used for ethanolic extract and serve in qualitative, quantitative and was appropriate for standardization of the plant.

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Optimasi Waktu Maserasi untuk Manggis (Garcinia mangostana L.) Rind Menggunakan Pelarut Etil Asetat

JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA ◽

10.20473/jfiki.v3i1.4087 ◽

2017 ◽

Vol 3 (1) ◽

pp. 12

Author(s):

Ni Putu Ayu Dewi Wijayanti ◽

Dewi LPMK ◽

Astuti KW ◽

Fitri NPE

Keyword(s):

Stationary Phase ◽

Ethyl Acetate ◽

Extraction Method ◽

Mobile Phase ◽

Freeze Drying ◽

Drying Method ◽

High Concentration ◽

Garcinia Mangostana ◽

The Difference ◽

Chloroform Methanol

Background: Maceration is a non-heating extraction method the result of which is affected by the type of solvent and the maceration time. Objective: To get the higher alpha-mangostin concentration (Garcinia mangostana L.) rind that was macerated using ethyl acetate solvent and to determine the optimum maceration time needed to produce high concentration of alpha-mangostin. Methods: the mangosteen rind was macerated using ethyl acetate with the variation of the maceration time of 6, 12, 24 and 48 hours. The extract was then dried using the freeze drying method. The optimum maceration time was determined by looking at the highest concentration of alpha-mangostin from each extraction time using TLC-densitometry method with a silica gel stationary phase of GF254 and a mobile phase of chloroform—methanol (10:0.1, v/v). Results: The results showed that the difference in the maceration time can affect the concentration of alpha-mangostin compound contained in the extract. Conclusions: It was found out that the 24-hour maceration time produced the highest concentration of alpha-mangostin that is 3031.34 ng.

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Chromatographic Separation of the Novel Hypoglycemic Drug Mitiglinide in its Bulk Powder and Pharmaceutical Formulation: Forced Degradation and Validated Stability-Indicating HPTLC/Densitometry and HPLC/UV Methods

Journal of AOAC International ◽

10.1093/jaocint/qsz004 ◽

2020 ◽

Vol 103 (3) ◽

pp. 736-742

Author(s):

Maya S Eissa ◽

Eman Darweish ◽

Mohammed R Elghobashy ◽

Mostafa A Shehata

Keyword(s):

Stationary Phase ◽

Flow Rate ◽

Mobile Phase ◽

Hplc Method ◽

Forced Degradation ◽

Stability Indicating ◽

Relative Standard ◽

Hypoglycemic Drug ◽

Hptlc Method

Abstract Background Mitiglinide (MTG) is one of meglitinides group which are used for treatment of type two diabetes mellitus. Objective Mitiglinide (MTG) is a novel oral hypoglycemic drug. The present work adopts two stability-indicating chromatographic methods for determination of MTG after being exposed to forced degradation using 4 M methanolic HCl for 12 h. Methods The first method is HPTLC/densitometry using methanol:chloroform:acetic acid (5:2.5:0.3 by volume) as the eluting system and silica gel 60 GF254 as the stationary phase; the separated bands were then scanned at 220 nm. The second method is HPLC/UV in which acetonitrile:methanol:0.05 M potassium dihydrogen orthophosphate (pH 3.5) (40:25:35 by volume) was used as the mobile phase and a Zorbax SB-C8 (250 × 4.6 mm, 5 µm) column as a stationary phase, at a flow rate of 1 mL/min and UV detection at 220 nm. Results As a result of acid hydrolysis, two degradants were obtained. The first one was benzyl succinic acid to which this study was performed. The second one lacked configuration and was unreadable using UV spectrometry. Linearity was in the range of 8–48 µg/band MTG for HPTLC and 10–80 µg MTG for HPLC. LOD and LOQ values were 1.85 and 5.62 µg/band for the HPTLC method and 2.14 and 6.49 µg/mL for the HPLC method, respectively. The Recovery % was 100.03 ± 1.464 and 99.61 ± 1.44 using the HPTLC and HPLC methods, respectively. The relative standard deviations (RSD, %) for intra- and inter-day assays were 1.111 and 1.430 for the HPTLC method, respectively, and those for the HPLC method were 1.377 and 0.866, respectively. The RSD, %, for robustness testing was 1.162 (saturation time of mobile phase) and 1.592 (change in ratio of methanol content) for the HPTLC method and 1.377 (mobile phase composition), 1.713 (detector wavelength) and 1.770 (mobile phase flow rate) for the HPLC method. Conclusions The adopted methods were successfully applied for the determination of MTG in its pure form, in presence of its acid degradant and in its tablet dosage form. Highlights Statistical comparison between the results obtained from the developed methods and those obtained by the reported HPLC method showed no significance difference.

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