A New Osteoporosis Prevention Supplements-diet Improve Bone Mineral Density in Ovariectomized Rats on Micro-CT

Objective: The aim of this study was to evaluate the potential effect of the methanolic extract of plantGlycyrrhiza glabraroots on bone mineral density and femoral bone strength of ovariectomized rats.Methods: Thirty 10-month-old Wistar rats were randomly separated into three groups of ten, Control, Ovariectomy and Ovariectomy-plus-Glycyrrhiza in their drinking water. Total and proximal tibial bone mineral density was measured in all groups before ovariectomy (baseline) and after 3 and 6 months post ovariectomy. Three-point-bending of the femurs and uterine weight and histology were examined at the end of the study.Results: No significant difference was noted in bone density percentage change of total tibia from baseline to 3 months between Control and Ovariectomy-plus-Glycyrrhiza groups (+5.31% ± 4.75 and +3.30% ± 6.31 respectively,P = non significant), and of proximal tibia accordingly (+5.58% ± 6.92 and +2.61% ± 13.62,P = non significant) demonstrating a strong osteoprotective effect. There was notable difference in percentage change of total tibia from baseline to 6 months between groups Ovariectomy and Ovariectomy-plus-Glycyrrhiza (−13.03% ± 5.11 and −0.84% ± 7.63 respectively,P < 0.005), and of proximal tibia accordingly (−27.9% ± 3.69 and −0.81% ± 14.85 respectively,P < 0.001), confirming the protective effect ofGlycyrrhiza glabraextract in preserving bone density of the Ovariectomy-plus-Glycyrrhiza group. Three-point-bending did not reveal any statistically significant difference between Ovariectomy and Ovariectomy-plus-Glycyrrhiza groups. Uterine weights of the Ovariectomy-plus-Glycyrrhiza group ranged between the other two groups with no statistically significant difference to each.Conclusions:Glycyrrhiza glabraroot extract notably protected tibial bone mineral density loss in Ovariectomy-plus-Glycyrrhiza rats in comparison with ovariectomized rats, but did not improve biomechanical strength.

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Electrical field stimulation improves bone mineral density in ovariectomized rats

Brazilian Journal of Medical and Biological Research ◽

10.1590/s0100-879x2006005000030 ◽

2006 ◽

Vol 39 (11) ◽

Author(s):

A.P.R. Lirani-Galvão ◽

C.T. Bergamaschi ◽

O.L. Silva ◽

M. Lazaretti-Castro

Keyword(s):

Bone Mineral Density ◽

Bone Mineral ◽

Electrical Field Stimulation ◽

Ovariectomized Rats ◽

Field Stimulation ◽

Mineral Density ◽

Electrical Field

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Recovery Effects of a 180 mT Static Magnetic Field on Bone Mineral Density of Osteoporotic Lumbar Vertebrae in Ovariectomized Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2011/620984 ◽

2011 ◽

Vol 2011 ◽

pp. 1-8 ◽

Cited By ~ 18

Author(s):

Shenzhi Xu ◽

Hideyuki Okano ◽

Naohide Tomita ◽

Yoshito Ikada

Keyword(s):

Magnetic Field ◽

Bone Mineral Density ◽

Bone Mineral ◽

Static Magnetic Field ◽

Lumbar Vertebrae ◽

Exposed Group ◽

Female Rats ◽

Ovariectomized Rats ◽

Moderate Intensity ◽

Mineral Density

The effects of a moderate-intensity static magnetic field (SMF) on osteoporosis of the lumbar vertebrae were studied in ovariectomized rats. A small disc magnet (maximum magnetic flux density 180 mT) was implanted to the right side of spinous process of the third lumbar vertebra. Female rats in the growth stage (10 weeks old) were randomly divided into 4 groups: (i) ovariectomized and implanted with a disc magnet (SMF); (ii) ovariectomized and implanted with a nonmagnetized disc (sham); (iii) ovariectomized alone (OVX) and (vi) intact, nonoperated cage control (CTL). The blood serum 17--estradiol (E2) concentrations were measured by radioimmunoassay, and the bone mineral density (BMD) values of the femurs and the lumbar vertebrae were assessed by dual energy X-ray absorptiometry. The E2concentrations were statistically significantly lower for all three operated groups than those of the CTL group at the 6th week. Although there was no statistical significant difference in the E2concentrations between the SMF-exposed and sham-exposed groups, the BMD values of the lumbar vertebrae proximal to the SMF-exposed area statistically significantly increased in the SMF-exposed group than in the sham-exposed group. These results suggest that the SMF increased the BMD values of osteoporotic lumbar vertebrae in the ovariectomized rats.

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Newly Formed Bone around Implanted New Titanium Alloy: Ti-15%Zr-4%Nb-4%Ta

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.661 ◽

2007 ◽

Vol 361-363 ◽

pp. 661-664

Author(s):

Hiroshi Nakada ◽

Yasuko Numata ◽

Taketoshi Suwa ◽

Y. Okazaki ◽

Racquel Z. LeGeros ◽

...

Keyword(s):

Mechanical Properties ◽

Bone Mineral Density ◽

Titanium Alloy ◽

Surface Treatment ◽

Bone Mineral ◽

Ti Alloy ◽

Micro Ct ◽

Mineral Density ◽

Biological Safety ◽

Biological Performance

We have developed a new Ti alloy, Ti-15%Zr-4%Nb-4%Ta alloy (Ti-15-4-4) that showed higher biological safety and mechanical properties than the currently used Ti-6%Al-4%V alloy. The purpose of this study is to determine the biological performance of the new alloy. Ti-15-4-4 implants (machined or blasted) were placed in surgically created defects in rabbit femurs. The rabbits were sacrificed after 4, 8, 16, 24 and 48 weeks. Bone mineral density (BMD) and area of newly formed bone around the implants were measured using micro-CT. Results showed that the Ti-15-4-4 alloy is biocompatible and forms new bone around the Ti-15-4-4 implant, regardless of the surface treatment. The BMD and area of newly formed bone around the blasted implant surfaces were significantly greater than those around the machined surfaces. These results indicate that the new Ti-15-4-4 alloy has a potential for use as implants and has the advantage of improved mechanical properties described in earlier studies.

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