LEAD AND CADMIUM CONCENTRATIONS IN SHEEP (OVIS ARIES) BLOOD SAMPLES FROM POLLUTED AREA AND THEIR ECOTOXICOLOGICAL INTERACTIONS WITH HEPATIC PROFILE

AbstractChanges in circadian rhythms of dROMs, Oxy-ads and SHp during reproductive stages were studied in Comisana ewes. Twelve ewes were divided in two equal groups. The experimental group consisted of ewes undergoing gestation and lactation following artificial insemination and the control group consisted of non-pregnant ewes. Blood samples were collected every 3 h over a 24 h period, 20 days before insemination, on days 100 and 140 of pregnancy, on days 10, 30 and 200 post-partum and during the dry period. In the control group, blood samples were collected on the same days and with the same procedures as those used for the experimental group. A significant effect of time on all parameters studied was observed in the experimental group. Daily rhythms of the parameters studied were observed in the control group in all experimental conditions, and in the experimental group during pre-pregnancy and dry periods. We conclude that the reproductive status of sheep affects oxidative stress markers in blood and their circadian rhythms.

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Blood lead and cadmium levels associated with hematological and hepatic functions in patients from an e-waste-polluted area

Chemosphere ◽

10.1016/j.chemosphere.2018.12.129 ◽

2019 ◽

Vol 220 ◽

pp. 531-538 ◽

Cited By ~ 11

Author(s):

Yanrong Chen ◽

Xijin Xu ◽

Zhijun Zeng ◽

Xueqiong Lin ◽

Qilin Qin ◽

...

Keyword(s):

Blood Lead ◽

Polluted Area ◽

Lead And Cadmium

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Blood haemogram in Ovis aries and Capra hyrcus: effect of storage time

Canadian Journal of Animal Science ◽

10.1139/cjas-2015-0106 ◽

2016 ◽

Vol 96 (1) ◽

pp. 32-36 ◽

Cited By ~ 2

Author(s):

Francesco Fazio ◽

Giuseppe Giangrosso ◽

Simona Marafioti ◽

Elisa Zanghì ◽

Francesca Arfuso ◽

...

Keyword(s):

Repeated Measures ◽

Storage Time ◽

Hematological Parameters ◽

Storage Conditions ◽

Ovis Aries ◽

Blood Storage ◽

Blood Samples ◽

Repeated Measures Analysis ◽

Haematological Profile ◽

Blood Storage Time

The aim of the present study was to assess the effect of storage time at +4 °C on haematological profile in goat (n = 25) and sheep (n = 25). After collection, blood samples were immediately analyzed and then divided into four aliquots that were stored at 4 °C and tested at 24 h (T1), 48 h (T2), 72 h (T3), and 1 wk (T4), respectively. One-way repeated-measures analysis of variance (ANOVA) was used to determine statistically significant effect of storage conditions both in goats and in sheep. Our results showed that among the two species studied, goats showed highest blood stability after refrigeration at +4 °C. In goats, all hematological parameters, except PLT, showed no significant changes during all days of monitoring with respect to basal values (T0). In sheep, no significantly effect of storage time on RBC and WBC levels were found, whereas the other hematological parameters change significantly over the time. Our findings suggest that the blood storage time reported for goat may not be applied to sheep’s blood, which underscore the differences between these two species that are erroneously considered similar.

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Erratum to: Preconcentration and determination of lead and cadmium levels in blood samples of adolescent workers consuming smokeless tobacco products in Pakistan

Environmental Monitoring and Assessment ◽

10.1007/s10661-015-4724-y ◽

2015 ◽

Vol 187 (8) ◽

Cited By ~ 1

Author(s):

Sadaf Sadia Arain ◽

Tasneem Gul Kazi ◽

Hassan Imran Afridi ◽

Kapil Dev Brahman ◽

Naeemullah ◽

...

Keyword(s):

Smokeless Tobacco ◽

Tobacco Products ◽

Blood Samples ◽

Lead And Cadmium ◽

Smokeless Tobacco Products

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Mitochondrial 16S rRNA Gene-Dependent Blood Typing as a Forensic Tool

10.5772/intechopen.98248 ◽

2021 ◽

Author(s):

Hussein O.M. Al-Dahmoshi ◽

Hayder J. Al-Nayili

Keyword(s):

Mitochondrial Dna ◽

16S Rrna ◽

16S Rrna Gene ◽

Bos Taurus ◽

Homo Sapiens ◽

Cross Reactivity ◽

Ovis Aries ◽

Capra Hircus ◽

Rrna Gene ◽

Blood Samples

Mitochondrial DNA is an important tool for human identification and is used to differentiate between human and animal blood at the crime scene, because in extreme conditions nuclear DNA is severely destroyed while Mitochondrial DNA contains multiple copies (200–2000) per cell and resists harsh and more stable conditions. Seventy-two blood samples were collected from humans (Homo sapiens), sheep (Ovis aries), goats (Capra hircus), and cows (Bos taurus) (18 blood samples for each). All blood samples were withdrawn by a technician and 5 ml were aspirated using an aseptic technique and transferred to EDTA-Na2 tubes. They were mixed well and stored in a refrigerator. The collection took 2 weeks (May 15, 2019–May 30, 2019). All samples were collected from Al-Diwanyia city. The results of PCR testing revealed that the primer pairs were specific and non-specific products did not appear for all samples. The amplification of Homo sapiens mitochondrial DNA with primer pairs of other (Ovis aries, Capra hircus, and Bos taurus) and amplification of each with primer pairs of another genus gave negative results, and this is primary evidence for primer pair specificity. The amplicon of 16S rRNA gene of Homo sapiens was 1200 bp, Ovis aries was 1060 bp, Capra hircus was 820 bp, and Bos taurus was 1300 bp. The sequencing revealed that no cross-reactivity of designed primer pairs and the PCR assay based on the designed primer pairs will be simple, fast, sensitive, specific, and cost-effective. There is sensitivity, specificity, and accuracy in the designed species-specific primer pairs and applicability of the designed primer pairs in forensics to investigate blood spots or evidence belonging for human, sheep, goat, and cow.

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The Effect of Heavy Metal Pollution on Some Haematological Parameters in Domestic Birds: A Study in Camp2 Village, Akamkpa Local Government Area of Cross River State, Nigeria

Bio-Research ◽

10.4314/br.v19i1.5 ◽

2021 ◽

Vol 19 (1) ◽

pp. 1217-1226

Author(s):

Ofem Effiom Eteng ◽

Grace Ekpo Imo ◽

Enobong Esiet Joe ◽

Ceaser Antiya Moses ◽

Kenyoh Eteng Abam ◽

...

Keyword(s):

Heavy Metals ◽

Venous Blood ◽

Control Site ◽

Blood Samples ◽

Cross River ◽

Cross River State ◽

Average Value ◽

Lead And Cadmium ◽

Domestic Birds ◽

Standard Limit

This study was conducted to evaluate the concentrations of heavy metals in the blood of birds reared for food in Camp2 Community of Akamkpa, Cross River State, Nigeria. Twelve (12) male indigenous jungle fowls (Gallus domestica), six each from the control site (Ugep) and from Camp2 were sampled for the studies. Venous blood samples from the wings of the blood were collected and used for hematological and metal analysis. After digestion of the blood, heavy metals [cadmium (Cd), Lead (Pb), Mercury (Hg), Chromium (Cr), Arsenic (As), and Nickel (Ni)] were detected using atomic absorption spectrophotometer (AAS). The results indicated that Cd, Pb, As and Hg) levels in birds from camp2 village was significantly higher (p<0.05; p = 0.0070) than those of the Ugep control site. The average value of nickel in the blood of Camp2 birds (4.72+1.32mg/L) was significantly higher (p>0.05; p= 0.003) than that of the Ugep control site (4.22+1.07mg/L). The result shows that nickel, lead and Cadmium in blood samples were significantly (p<0.05; p = 0.023) higher in Camp2 compared to the control site. Hence, these values were above WHO standard (0.1mg/l) and FAO/WHO standards of (0.2mg/kg) while others were within joint FAO/WHO standard limit. The studies show that domestic birds can bioaccumulate heavy metals in their blood, which in turn, presents serious health risks to humans who consume these birds within the communities.

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Molecular detection of Anaplasma spp., Babesia spp. and Theileria spp. in yaks (Bos grunniens) and Tibetan sheep (Ovis aries) on the Qinghai-Tibetan Plateau, China

Parasites & Vectors ◽

10.1186/s13071-021-05109-2 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Yongcai He ◽

Wangkai Chen ◽

Ping Ma ◽

Yaoping Wei ◽

Ruishan Li ◽

...

Keyword(s):

Ovis Aries ◽

Babesia Bovis ◽

Tibet Plateau ◽

Nested Polymerase Chain Reaction ◽

Anaplasma Ovis ◽

Blood Samples ◽

Qinghai Province ◽

Bos Grunniens ◽

Tibetan Sheep ◽

Qinghai Tibet Plateau

Abstract Background Anaplasma, Babesia and Theileria are tick-borne pathogens (TBPs) that affect livestock worldwide. However, information on these pathogens in yaks (Bos grunniens) and Tibetan sheep (Ovis aries) on the Qinghai-Tibet Plateau (QTP), China, is limited. In this study, Anaplasma spp., Babesia spp. and Theileria spp. infections were assessed in yaks and Tibetan sheep from Qinghai Province. Methods A total of 734 blood samples were collected from 425 yaks and 309 Tibetan sheep at nine sampling sites. Standard or nested polymerase chain reaction was employed to screen all the blood samples using species- or genus-specific primers. Results The results showed that 14.1% (60/425) of yaks and 79.9% (247/309) of Tibetan sheep were infected with at least one pathogen. Anaplasma ovis, Anaplasma bovis, Anaplasma capra, Anaplasma phagocytophilum, Babesia bovis and Theileria spp. were detected in this study, with total infection rates for all the assessed animals of 22.1% (162/734), 16.3% (120/734), 23.6% (173/734), 8.2% (60/734), 2.7% (20/734) and 19.3% (142/734), respectively. For yaks, the infection rate of A. bovis was 6.4% (27/425), that of B. bovis was 4.7% (20/425) and that of Theileria spp. was 3.3% (14/425). Moreover, 52.4% (162/309) of the Tibetan sheep samples were infected with A. ovis, 30.1% (93/309) with A. bovis, 56.0% (173/309) with A. capra, 19.4% (60/309) with A. phagocytophilum and 41.4% (128/309) with Theileria spp. Conclusions This study revealed the prevalence of Anaplasma spp., Babesia spp. and Theileria spp. in yaks and Tibetan sheep in Qinghai Province, China, and provides new data for a better understanding of the epidemiology of TBPs in these animals in this area of the QTP, China. Graphical Abstract

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