scholarly journals Diagnostic signs of morphological and anatomical structure of Medicago falcata L. raw materials

2020 ◽  
Vol 0 (3(64)) ◽  
pp. 51-57
Author(s):  
A. Garibli ◽  
T. Suleymanov ◽  
Y. Kerimov ◽  
E. Gasimov ◽  
F. Rzayev
Keyword(s):  
Raw Materials ◽  
Anatomical Structure ◽  
Medicago Falcata ◽  
Diagnostic Signs

STUDY OF MICROSCOPIC FEATURES OF TANACETUM SANTOLINA PLANT

2022 ◽  
pp. 38-44
Author(s):  
М.С. Жанибек ◽  
К.К. Орынбасарова
Keyword(s):  
Essential Oil ◽  
Calcium Oxalate ◽  
Raw Materials ◽  
Microscopic Analysis ◽  
Anatomical Structure ◽  
Raw Material ◽  
Plant Fibers ◽  
Oil Glands ◽  
Plant Raw Materials ◽  
Diagnostic Signs

В данной статье представлены результаты микроскопического исследования особенностей анатомического строения (листья, цветки) растения пижмы сантолинной (Tanacetum Santolina). Цель работы: изучение анатомического строения надземных органов, определение подлинности и отличия от других видов. Материалы и методы: объект исследования - цветки пижмы сантолинной, собранные в период цветения в мае 2020 года в предгорных равнинах Каратау. Микроскопическое исследование цветков пижмы сантолинной проводилось в соответствии с требованиями государственной фармакопеи Республики Казахстан. С помощью микроскопа Биолам-5 изучался объект с увеличением от х40 до х400. Исследование срезов и создание микрорисунков проводили с помощью цифрового камерного тринокулярного микроскопа «МТ300L» (Япония) (увеличенный 7х1,5х4,5; 7х1,5х8; 7х1,5х40). Результаты и обсуждения: изучены диагностические признаки для определения цельного сырья: диагностические признаки листьев, форма клеток эпидермиса, тип аппарата устьиц, строение волосков и эфирномасличных желез; формы оксалата кальция. Выводы: проведен микроскопический анализ цветков пижмы сантолинной. Для определения подлинности этого сырья были выявлены анатомодиагностические признаки. Во всех частях растения встречаются одноклеточные волоски и пучковые волокна. На обеих частях листовой пластины хорошо видны волокна и волоски, устьица, пигментные полости. Кристаллы оксалата кальция, находятся в мезофильных полостях листа. В цветках оксалат кальция встречается только в виде четырехугольного кристалла. На анатомическом признаке измельченного сырья видны эфирномасличные железки. Форма эфирно-масличных желез обнаруживает основные диагностические признаки цветков и листьев пижмы сантолинной. Анатомические характеристики позволяют повысить уровень стандартизации, дальнейшее изучение лекарственного растительного сырья This article presents the results of a microscopic study of the anatomical structure (leaves, flowers) of the Santolina’s tansy plant (Tanacetum Santolina). The purpose of the work: to study the anatomical structure of aerial organs, to determine authenticity and differences from other species. Materials and methods: the object of research is the flowers of Santolina’s tansy collected during the flowering period in May 2020 in the foothill plains of Karatau. Microscopic examination of the flowers of Santolina’s tansy was carried out in accordance with the requirements of the State Pharmacopoeia of the Republic of Kazakhstan. Using a Biolam-5 microscope with magnification from x40 to x400 an object was studied. The study of sections and the creation of micro-nozzles was carried out using a digital chamber trinocular microscope "MT300L" (Japan) (enlarged 7x1.5x4.5; 7x1.5x8; 7x1.5x40). Results and discussions: diagnostic signs for the determination of whole raw materials were studied: diagnostic signs of leaves, the shape of epidermis cells, the type of stomatal apparatus, the structure of hairs and essential oil glands; forms of calcium oxalate. Conclusions: microscopic analysis of flowers of Santolina’s tansy was carried out. Anatomical and diagnostic signs were identified to determine the authenticity of this raw material. Unicellular hairs and beam fibers are found in all parts of the plant. Fibers and hairs, stomata, pigment cavities are clearly visible on both parts of the leaf plate. Calcium oxalate crystals are located in the mesophilic cavities of the leaf. In flowers, calcium oxalate occurs only in the form of a quadrangular crystal. On the anatomical feature of the crushed raw materials, essential oil glands are visible. The shape of the essential oil glands reveals the main diagnostic signs of flowers and leaves of santolina tansy. Anatomical characteristics make it possible to increase the level of standardization, further study of medicinal plant raw materials.

Microscopic Evaluation of Salvia stepposa Des.- Schost above - ground part widespread in Kazakhstan

2021 ◽  
pp. 4773-4776
Author(s):  
Levaya Ya. K. ◽  
Ishmuratova M. Yu. ◽  
Atazhanova G. A. ◽  
Zilfikarov I. N. ◽  
Loseva I. V.
Keyword(s):  
Raw Materials ◽  
Microscopic Analysis ◽  
Raw Material ◽  
Medical Systems ◽  
Anatomical Structures ◽  
Anatomical Features ◽  
Mesophyll Structure ◽  
Microscopic Evaluation ◽  
Diagnostic Signs ◽  
Ground Part

Microscopic analysis of medicinal plants and raw materials is one of the stages of quality control which makes it possible to determine the authenticity of an object. This article presents the results of microscopic analysis of leaves, corolla, leafstalks and stalk of Salvia stepposa (steppe sage). This species is a relative to Salvia officinalis officinal used in traditional medical systems as an anti-inflammatory agent. The purpose of this study is to determine the features of the anatomical structures of the above-ground part of steppe sage and to reveal diagnostic signs at the microscopic level. Preliminary pharmacognostic investigation of vegetative raw material is done and diagnostic signs are identified. The detail microscopy revealed the presence of 3 types of trichomes on leaves’ epidermis: 1) simple unicellular; 2) simple multicellular; 3) glandular; their localization and shape are determined. Type of mesophyll, structure of trichomes; form and location of epidermal cells, the presence of rare simple trichomes along the edge are discovered. As a result of the research the anatomical features of the raw material were established, which make it possible to identify the above-ground part of Salvia stepposa and can be used for the development of regulatory documentation.

scholarly journals Application of Luminescent Microscopy in Analysis of Anatomic-diagnostic Signs of Fruits of Sea Buckthorn

2020 ◽  
Vol 9 (1) ◽  
pp. 40-45
Author(s):  
O. V. Trineeva ◽  
A. A. Gudkova ◽  
M. A. Rudaya
Keyword(s):  
Medicinal Plant ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Sea Buckthorn ◽  
Biologically Active ◽  
Plant Materials ◽  
Fatty Oil ◽  
Luminescent Analysis ◽  
Diagnostic Signs ◽  
Promising Source

Introduction. Sea buckthorn (Hippophae rhamnoides), the family Eleagnaceae – a promising source of biologically active substances, the traditional raw materials of which are fruits. The existing regulatory documentation (ND) lacks such an indicator of the authenticity and good quality of the fruit as «microscopy». Despite the available data on the anatomy and histology of the fruits of this type of medicinal plant material (MPM), the scientific literature does not describe the luminescence features of the tissues of sea buckthorn fruit. It is known that the luminescent features of tissues make it possible to identify the localization of biologically active structures, and also, in some cases, to conduct selective diagnosis of MPM.Aim. The aim of this work was to study the characteristics of the luminescence of the tissues of dried crushed fruits of sea buckthorn, and also to optimize the conditions for analysis.Materials and methods. The object of the study was the dried, crushed fruits of sea buckthorn of various species. The study of microdiagnostic signs was carried out according to the State Pharmacopoeia of the XIV RF GPA.1.5.3.0003.15 «Technique of microscopic and microchemical studies of medicinal plant materials and herbal medicines». A stereomicroscopic study was carried out on a Biomed-6 microscope. The luminescence of sea buckthorn fruit tissues was examined using a luminescent microscope of the Micromed-3 Lum brand.Results and discussion. The most pronounced yellow glow is observed for the tissue of the pulp and epidermis of the fetus, which is associated with the highest content of fatty oil in these structures. Groups of phenolic compounds contained along with oil give a greenish tint to objects, while condensed tannins give a brownish tint. The plates that make up the corymbose hairs do not exhibit bright luminescence; a weak glow is characteristic of the joints of the hair platelets. The walls of stellate hairs have a faint greenish glow. Fragments of the actual fruit («sac») are brownish in color, without their own luminescence, as well as the seed peel. The embryo has its own greenish luminescence due to the presence of both a fatty oil and a complex of storage substances.Conclusion. The luminescent analysis of sea buckthorn fruits was carried out for the first time. The choice of a method for preparing the studied MPM for microscopic examination is experimentally substantiated. The luminescent analysis revealed the peculiarities of the glow of the tissues of the fruits of sea buckthorn. The main microdiagnostic signs of crushed dried sea buckthorn fruits and their biometric characteristics are specified. The analysis of the obtained data will allow us to further develop the section «Microscopic signs» for inclusion in the PA project on MPM, widely cultivated and used by the domestic pharmaceutical industry for the production of herbal medicines.

scholarly journals Comparative anatomical structure of the leaf Rhododendron catawbiense Michx. and Rhododendron brachycarpum D. Don, growing under conditions of N.V. Tsitsin Main Botanical Garden of the Russian Academy of Sciences

2021 ◽  
Vol 10 (1) ◽  
pp. 33-40
Author(s):  
Yulia Vladimirovna Bondar ◽  
Sergey Vladimirovich Zerkal ◽  
Nelly Vladimirovna Hetko ◽  
Sergey Lvovich Rysin
Keyword(s):  
Far East ◽  
Botanical Garden ◽  
Anatomical Structure ◽  
Administrative District ◽  
Natural Habitats ◽  
Russian Academy Of Sciences ◽  
Key Indicator ◽  
Diagnostic Signs ◽  
Growing Conditions ◽  
Academy Of Sciences

The paper deals with anatomical parameters of the leaf of two genus representatives Rhododendron L.: R. catawbiense Michx. and R. brachycarpum D. Don, cultivated under conditions of N.V. Tsitsin Main Botanical Garden of the Russian Academy of Sciences. The morpho-anatomical structure of the leaf, used in the diagnosis and taxonomy of species, is also a key indicator of its functional characteristics. It is no less important for studying the mechanisms of their adaptation during introduction into new growing conditions and allows to find ways to optimize the conditions of their life and cultivation. Introduction experience is a prerequisite for studying the adaptive potential of species and for developing methods for managing the growth and development of these plants. The paper provides a detailed ecological and geographical characteristics of natural growth areas of R. catawbiense (eastern part of North America), R. brachycarpum (Far East, Republic of Korea, Japan) and the introduction area of Moscow (North-Eastern Administrative District, N.V. Tsitsin Main Botanical Garden of the Russian Academy of Sciences). In this work, for the first time, a detailed anatomical and morphological analysis of the leaf structure of two genus representatives Rhododendron: R. catawbiense and R. brachycarpum was carried out, and diagnostic signs of changes in the parameters of the structure of leaves, formed in conditions different from the natural habitats of species, were revealed during their introduction into N.V. Tsitsin Main Botanical Garden of the Russian Academy of Sciences.

scholarly journals Comparative characteristics of the anatomical structure of leaves of Allium bidentatum and A. polyrrhizum

2020 ◽  
Author(s):  
O.I. Zhapova ◽  
◽  
T.P. Antsupova ◽  
Keyword(s):  
Anatomical Structure ◽  
Comparative Characteristic ◽  
Diagnostic Signs

The article provides a comparative characteristic of the anatomical structure of leaves and stems of Allium bidentatum, Fisch. ex Prokh. and Allium polyrrhizum Turcz. ex Regel. As diagnostic signs of the anatomical structure in determining the species, you can use the features of the location and structure of the conductive bundles.

scholarly journals Microscopical Investigation of Sideritis taurica

2020 ◽  
Vol 3 (3) ◽  
pp. 162-169
Author(s):  
Fatima Kazbekovna Serebryanaya ◽  
Irina Konovalova
Keyword(s):  
Water System ◽  
Anatomical Study ◽  
Anatomical Structure ◽  
Ornamental Plant ◽  
Plant Identification ◽  
Microscopical Investigation ◽  
Vegetative Organs ◽  
Research Material ◽  
Central Regions ◽  
Diagnostic Signs

This research presents the results of the morphological-anatomical study of grass organs above ground (leaves, stems, and flowers) from Sideritis taurica Steph. ex Willd. from the Lamiaceae family. The range of distribution of this species covers Eastern Europe, including Crimea, Caucasus. This plant is commonly found as an ornamental plant and grows in the southern and central regions of Russia. The purpose of this study is the anatomical and morphological study of the vegetative organs of S. taurica and the formation of diagnostic signs for reliable plant identification. The research material used was S. taurica herbicide samples collected in Crimea on the slopes of Mount Ay-Petri. Plant material is fixed in the glycerin : water system in a ratio of 1 : 1. Cross-section of stems and leaves is carried out using blades. The study was conducted with the morphological-anatomical method. The results obtained indicate the anatomical structure of the lamina, stem, and flower of the plant that has been studied. Anomocytic types of stomatal devices, unicellular and multicellular trichomes, located on the upper and lower sides of the leaf, as well as on the corolla stem and epidermis, have been discovered. The study carried out is part of the S. taurica complex pharmacognostic study.

scholarly journals MICROMORPHOLOGICAL ANALYSIS OF THE AERIAN ORGANES OF MARRUBIUM ALYSSON L. FROM FLORA OF TUNISIA

2018 ◽  
Vol 6 (5) ◽  
pp. 462-474
Author(s):  
F. K. Serebryanaya ◽  
G. Zayer
Keyword(s):  
Raw Materials ◽  
Anatomical Structure ◽  
Central Vein ◽  
Herbarium Specimens ◽  
Anatomic Structure ◽  
Histochemical Methods ◽  
Stomatal Apparatus ◽  
Epidermis Structure ◽  
Product Specification ◽  
Micromorphological Analysis

The micromorphological investigations ofMarrubium alysson L.growing on the territory of the Tunisian Republic have been conducted.The aimof the study was to conduct comparative ecological and morphological researches ofMarrubium alyssonaerian parts, to study an anatomical structure of caulifoliary system elements including stems, petioles, leaf margins a structure of the epidermis.Materials and methods.As an object of the research, dried-up herb and herbarium specimens ofMarrubium alysson L., growing on the territory of the Tunisian Republic, were used. The micromorphological investigations were conducted by morphological and histochemical methods.Results and discussion.Comparative ecological and morphological researches have been conducted. The anatomic structure of the elements of the caulifoliary system including stems, petioles, leaf margins has been studied. The anatomic structure of the epidermis has been studied, too. The diacytic type of the stomatal apparatus, numerous branched trichomes with multicellular basis, located both on top and lower leaf parts have been found out. The conducted researches are a fragment of complex researches of perspective raw plants of Tunisian flora.Conclusion. Comparative eсological and micromorphological researches have been conducted, the anatomic structure of the stems, petioles, leaf margins, and also the epidermis structure of top and lower leaf parts has been also carried out. The diacytic type of the stomatal apparatus, trichomes with multicellular basis located both on top and lower leaf parts have been found out. The collenchyme is located in the subepidermal zone of a stem, petioles and in the zone of the central vein of a limb. The revealed signs can be included into product specification files on herbal raw materials

scholarly journals The comparative anatomic morphological study of Bidens L. species

2011 ◽  
Vol 10 (5) ◽  
pp. 56-61 ◽  
Author(s):  
N. V. Isaykina ◽  
V. Yu. Andreyeva
Keyword(s):  
Morphological Study ◽  
Raw Materials ◽  
Anatomical Structure ◽  
Morphological Features ◽  
Anatomical Features

The purpose of research is to learn morphological and anatomical structure of Bidens L. types for elaboration of normative documentation's project of the herb bur-marigold. Morphological and anatomical features of production samples formal raw-materials and other Bidens L. types were explored by methods of pharmacopeia. There were revealed essential differences of these types' morphological features and conformity of their anatomical structure.

scholarly journals Identification of anise (Anisum vulgare Gaertn.) herbs by morphological and anatomical characteristics

2021 ◽  
Vol 14 (1) ◽  
pp. 39-44
Author(s):  
S. V. Kolisnyk ◽  
T. M. Gontova ◽  
U. A. Umarov ◽  
K. R. Hordiei
Keyword(s):  
Antibacterial Effect ◽  
Raw Materials ◽  
Microscopic Analysis ◽  
Anatomical Structure ◽  
Biologically Active ◽  
Professional Literature ◽  
Plant Raw Materials ◽  
Naked Eye ◽  
Normative Documentation ◽  
First Time

The fruits of anise contain different classes of biologically active compounds, due to which they have antidepressant, antiperoxidant, antidiabetic effect; extracts of this plant have an antibacterial effect. Professional literature lacks information on the chemical composition of anise herbs and prospects for their use in medicine. The aim of the work is to study of the morphological and anatomical structure of the aboveground organs of anise and to establish of macro- and microscopic diagnostic characters necessary for the identification of the plant raw materials. Materials and methods. Anise herbs consisting of stems, leaves flowers, and unripe fruits were used for the study. Fresh and dried raw materials were macroscopically analyzed with the naked eye and using a magnifying glass (×10). Studies of transverse and longitudinal sections, epidermis and surface preparations were performed using conventional techniques: with microscopes MBS 9, MS 10 (glasses ×5, ×10, ×15, lenses ×10, ×40), Micromed XS-4130 (eyepiece WF15X, lenses ×40/0.65, ×10/0.25) with a photomicrographic attachment. The micrographs were taken with Samsung PL50 camera. Results. As a result of the macro- and microscopic analysis, diagnostic properties of anise herbs were established. Conclusions. It is for the first time that macro- and microscopic characteristics of anise herbs were studied, which confirms the identity of raw materials. The obtained results will be used for the development of domestic normative documentation on anise herbs raw materials.

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