scholarly journals MICROMORPHOLOGICAL ANALYSIS OF THE AERIAN ORGANES OF MARRUBIUM ALYSSON L. FROM FLORA OF TUNISIA

2018 ◽  
Vol 6 (5) ◽  
pp. 462-474
Author(s):  
F. K. Serebryanaya ◽  
G. Zayer
Keyword(s):  
Raw Materials ◽  
Anatomical Structure ◽  
Central Vein ◽  
Herbarium Specimens ◽  
Anatomic Structure ◽  
Histochemical Methods ◽  
Stomatal Apparatus ◽  
Epidermis Structure ◽  
Product Specification ◽  
Micromorphological Analysis

The micromorphological investigations ofMarrubium alysson L.growing on the territory of the Tunisian Republic have been conducted.The aimof the study was to conduct comparative ecological and morphological researches ofMarrubium alyssonaerian parts, to study an anatomical structure of caulifoliary system elements including stems, petioles, leaf margins a structure of the epidermis.Materials and methods.As an object of the research, dried-up herb and herbarium specimens ofMarrubium alysson L., growing on the territory of the Tunisian Republic, were used. The micromorphological investigations were conducted by morphological and histochemical methods.Results and discussion.Comparative ecological and morphological researches have been conducted. The anatomic structure of the elements of the caulifoliary system including stems, petioles, leaf margins has been studied. The anatomic structure of the epidermis has been studied, too. The diacytic type of the stomatal apparatus, numerous branched trichomes with multicellular basis, located both on top and lower leaf parts have been found out. The conducted researches are a fragment of complex researches of perspective raw plants of Tunisian flora.Conclusion. Comparative eсological and micromorphological researches have been conducted, the anatomic structure of the stems, petioles, leaf margins, and also the epidermis structure of top and lower leaf parts has been also carried out. The diacytic type of the stomatal apparatus, trichomes with multicellular basis located both on top and lower leaf parts have been found out. The collenchyme is located in the subepidermal zone of a stem, petioles and in the zone of the central vein of a limb. The revealed signs can be included into product specification files on herbal raw materials

Features of the anatomy of the leaves of Laurocerasus officinalis M. Roem.

2020 ◽  
Author(s):  
Yu.S. Cheryatova ◽  
Keyword(s):  
Essential Oil ◽  
Leaf Blade ◽  
Raw Materials ◽  
Microscopic Analysis ◽  
Anatomical Structure ◽  
Diagnostic Features ◽  
Spongy Mesophyll ◽  
Plant Raw Materials ◽  
Stomatal Apparatus ◽  
Main Vein

The article presents the results of microscopic analysis of the leaves of L. officinalis. The main anatomical and diagnostic features that can be used in identifying and evaluating the authenticity of medicinal plant raw materials are established. Analysis of the anatomical structure showed that the leaves of L. officinalis are dorsoventral; the leaf plastic is hypostomatic, and the stomatal apparatus is anomocytic. The main vein of the leaf blade and petiole is represented by a bicollateral conducting bundle. Idioblasts represented by round- shaped essential oil cells were first identified in the columnar and spongy mesophyll of the leaf and petiole. Single diamond-shaped crystals and calcium oxalate druses were also found in the leaves. The information obtained can serve as a basis for the development of the section "Microscopy" in the draft regulatory documentation.

Systematics of the southern African genus Ixia (Iridaceae). 2. The filiform-leaved I. capillaris complex

Bothalia ◽  
2008 ◽  
Vol 38 (2) ◽  
pp. 115-124
Author(s):  
P. Goldblatt ◽  
J. C. Manning
Keyword(s):  
New Species ◽  
Field Study ◽  
South African ◽  
Central Vein ◽  
Herbarium Specimens ◽  
Supporting Evidence ◽  
Leaf Type ◽  
Perianth Tube ◽  
Lateral Branches ◽  
African Genus

Field study and associated examination of herbarium specimens of the filiform-leaved species of section Morphixia o f the South African genus Ixia L. have resulted in an increase in the number of species with this derived leaf type.  Ixia capillaris and  I. pauciflora have until now been the only species with such leaves and they have not been regarded as immediately related in past accounts of the genus. The two foliage leaves, typically less than 2 mm w ide, with a leathery to succulent texture, and lacking a raised central vein or margins, are specialized in the genus. Associated finely fibrous corm tunics, spikes of 1-3 flowers, and when present, short, thread-like lateral branches, usually bearing 1 or 2 flowers, provide supporting evidence that the group is monophyletic.  I. capillaris as interpreted until now. comprises four species, three of them new and described here, and the large-flowered I. pauciflora includes two species, one of these described here. While I. capillaris has a branched stem, radially symmetric flowers with a penanth tube (4—)5—7(—8) mm long, tepals 11-15 mm long and thus substantially exceeding the tube, filaments typically exserted 1-2 mm. and anthers (3—)4—5 mm long. I. exiliflora has a tube 8-10 mm long and ± as long as the tepals, included filaments, and anthers 3.5—4.0 mm long. The new  I. dieramoides also has included filaments but a perianth tube 13—18(—22) mm long and tepals 11-18 mm long. A third new species. I. reclinata has large flowers with a tube 13-15 mm long, tepals 16-21 mm long, and unilateral, decimate stamens with the filaments exserted 8-10 mm. and anthers 4-5 mm long. Typical  I. pauciflora has flowers with unilateral stamens and filaments exserted 2-6 mm from the flower and anthers prominently displayed, but specimens until now included in that species w ith short, included filaments 3-5 mm long and anthers half included in the tube, are here regarded as I. dieramoides. The I. capillaris group astreated here, now includes five species.

Revision of herbarium specimens of freshwater Enteromorpha-like Ulva (Ulvaceae, Chlorophyta) collected from Central Europe during the years 1849–1959

Phytotaxa ◽  
2015 ◽  
Vol 218 (1) ◽  
pp. 1 ◽  
Author(s):  
Andrzej Stanisław Rybak
Keyword(s):  
Detailed Analysis ◽  
Central Europe ◽  
Sea Water ◽  
Taxonomic Revision ◽  
Marine Species ◽  
Freshwater Ecosystems ◽  
Herbarium Specimens ◽  
Anatomic Structure ◽  
Inland Water ◽  
Water Ecosystems

This paper presents results concerning the taxonomic revision of Ulva taxa originating from herbarium specimens dating back to 1849–1959. A staining and softening mixture was applied to allow a detailed morphometric analysis of thalli and cells and to detect many morphological details. The study focused on individuals collected exclusively from inland water ecosystems (having no contact with sea water). Detailed analysis concerned the following items: structures of thalli and cells, number of pyrenoids per cell, configuration of cells inside the thallus, occurrence or not of branching thallus, shapes of apical cells, and shape of chromatophores. The objective of the study was to confirm the initial identifications of specimens of Ulva held in herbaria. The study sought to determine whether saltwater species of the genus Ulva, e.g., Ulva compressa and U. intestinalis, could have been found in European freshwater ecosystems in 19th and 20th centuries. Moreover, the paper presents a method for the initial treatment of voucher specimens for viewing stained cellular structures that are extremely vulnerable to damage (the oldest specimens were more than 160 years old). The use of a glycerine-based softening mixture allowed the detailed analysis of the anatomic structure of cells as well as the determination of the number and size of pyrenoids, i.e., some of the key taxonomic features of Ulva taxa. Most of specimens were identified as belonging to the species U. flexuosa. Marine species such as Ulva intestinalis or U. compressa were not confirmed to occur in the inland water ecosystems of Central Europe in the 19th and 20th centuries.

scholarly journals Anatomical structure of the stem Bidens tripartita (Asteraceae) in connection with habitat

2020 ◽  
Vol 19 (2) ◽  
pp. 168-172
Author(s):  
S. V. Shabalkina ◽  
E. A. Shuleva
Keyword(s):  
Internal Structure ◽  
Anatomical Structure ◽  
Biometric Parameters ◽  
Transitional Type ◽  
Bidens Tripartita ◽  
Zones Of Inhibition ◽  
Stomatal Apparatus

Internal structure of the stem in internodes of metamers middle and upper zones of inhibition, amplificationzone in generative individuals Bidens tripartita L. is described. It is of a transitional type, for a longer length – hollow.Epidermis with an anomocytic type of stomatal apparatus. Anatomical structure of the stem in plants from two communitieswas compared. Due to close biotope conditions, the biometric parameters of the structures do not differ.

Leaf anatomy of the South African Danthonieae (Poaceae). XIV. Pentameris dregeana

Bothalia ◽  
1986 ◽  
Vol 16 (2) ◽  
pp. 235-241
Author(s):  
R. P. Ellis
Keyword(s):  
Light Microscopy ◽  
South African ◽  
Leaf Anatomy ◽  
Representative Sample ◽  
Anatomical Structure ◽  
The Other ◽  
Herbarium Specimens ◽  
The South ◽  
Fixed Material

Transverse sections and abaxial epidermal scrapes of leaf blades of Pentameris dregeana Stapf, both of herbarium specimens and of freshly fixed material, were examined by light microscopy. The anatomical structure was found to be basically uniform in a representative sample. A few somewhat atypical specimens, how'ever, showed epidermal similarities with  Pentaschistis colorata (Steud.) Stapf. A comparison with other danthonoid grasses revealed some specimens identified as  Pentaschistis colorata var.  polytricha Stapf which resemble  Pentameris dregeana very closely in leaf anatomy. A definite gradation in leaf anatomy between  Pentameris dregeana and  Penta­schistis colorata appears to exist and. consequently, it is proposed that the affinities of Pentameris dregeana lie with this group of  Pentaschistis species rather than close to any of the other  Pentameris species.

STUDY OF MICROSCOPIC FEATURES OF TANACETUM SANTOLINA PLANT

2022 ◽  
pp. 38-44
Author(s):  
М.С. Жанибек ◽  
К.К. Орынбасарова
Keyword(s):  
Essential Oil ◽  
Calcium Oxalate ◽  
Raw Materials ◽  
Microscopic Analysis ◽  
Anatomical Structure ◽  
Raw Material ◽  
Plant Fibers ◽  
Oil Glands ◽  
Plant Raw Materials ◽  
Diagnostic Signs

В данной статье представлены результаты микроскопического исследования особенностей анатомического строения (листья, цветки) растения пижмы сантолинной (Tanacetum Santolina). Цель работы: изучение анатомического строения надземных органов, определение подлинности и отличия от других видов. Материалы и методы: объект исследования - цветки пижмы сантолинной, собранные в период цветения в мае 2020 года в предгорных равнинах Каратау. Микроскопическое исследование цветков пижмы сантолинной проводилось в соответствии с требованиями государственной фармакопеи Республики Казахстан. С помощью микроскопа Биолам-5 изучался объект с увеличением от х40 до х400. Исследование срезов и создание микрорисунков проводили с помощью цифрового камерного тринокулярного микроскопа «МТ300L» (Япония) (увеличенный 7х1,5х4,5; 7х1,5х8; 7х1,5х40). Результаты и обсуждения: изучены диагностические признаки для определения цельного сырья: диагностические признаки листьев, форма клеток эпидермиса, тип аппарата устьиц, строение волосков и эфирномасличных желез; формы оксалата кальция. Выводы: проведен микроскопический анализ цветков пижмы сантолинной. Для определения подлинности этого сырья были выявлены анатомодиагностические признаки. Во всех частях растения встречаются одноклеточные волоски и пучковые волокна. На обеих частях листовой пластины хорошо видны волокна и волоски, устьица, пигментные полости. Кристаллы оксалата кальция, находятся в мезофильных полостях листа. В цветках оксалат кальция встречается только в виде четырехугольного кристалла. На анатомическом признаке измельченного сырья видны эфирномасличные железки. Форма эфирно-масличных желез обнаруживает основные диагностические признаки цветков и листьев пижмы сантолинной. Анатомические характеристики позволяют повысить уровень стандартизации, дальнейшее изучение лекарственного растительного сырья This article presents the results of a microscopic study of the anatomical structure (leaves, flowers) of the Santolina’s tansy plant (Tanacetum Santolina). The purpose of the work: to study the anatomical structure of aerial organs, to determine authenticity and differences from other species. Materials and methods: the object of research is the flowers of Santolina’s tansy collected during the flowering period in May 2020 in the foothill plains of Karatau. Microscopic examination of the flowers of Santolina’s tansy was carried out in accordance with the requirements of the State Pharmacopoeia of the Republic of Kazakhstan. Using a Biolam-5 microscope with magnification from x40 to x400 an object was studied. The study of sections and the creation of micro-nozzles was carried out using a digital chamber trinocular microscope "MT300L" (Japan) (enlarged 7x1.5x4.5; 7x1.5x8; 7x1.5x40). Results and discussions: diagnostic signs for the determination of whole raw materials were studied: diagnostic signs of leaves, the shape of epidermis cells, the type of stomatal apparatus, the structure of hairs and essential oil glands; forms of calcium oxalate. Conclusions: microscopic analysis of flowers of Santolina’s tansy was carried out. Anatomical and diagnostic signs were identified to determine the authenticity of this raw material. Unicellular hairs and beam fibers are found in all parts of the plant. Fibers and hairs, stomata, pigment cavities are clearly visible on both parts of the leaf plate. Calcium oxalate crystals are located in the mesophilic cavities of the leaf. In flowers, calcium oxalate occurs only in the form of a quadrangular crystal. On the anatomical feature of the crushed raw materials, essential oil glands are visible. The shape of the essential oil glands reveals the main diagnostic signs of flowers and leaves of santolina tansy. Anatomical characteristics make it possible to increase the level of standardization, further study of medicinal plant raw materials.

scholarly journals Diagnostic signs of morphological and anatomical structure of Medicago falcata L. raw materials

2020 ◽  
Vol 0 (3(64)) ◽  
pp. 51-57
Author(s):  
A. Garibli ◽  
T. Suleymanov ◽  
Y. Kerimov ◽  
E. Gasimov ◽  
F. Rzayev
Keyword(s):  
Raw Materials ◽  
Anatomical Structure ◽  
Medicago Falcata ◽  
Diagnostic Signs

scholarly journals The comparative anatomic morphological study of Bidens L. species

2011 ◽  
Vol 10 (5) ◽  
pp. 56-61 ◽  
Author(s):  
N. V. Isaykina ◽  
V. Yu. Andreyeva
Keyword(s):  
Morphological Study ◽  
Raw Materials ◽  
Anatomical Structure ◽  
Morphological Features ◽  
Anatomical Features

The purpose of research is to learn morphological and anatomical structure of Bidens L. types for elaboration of normative documentation's project of the herb bur-marigold. Morphological and anatomical features of production samples formal raw-materials and other Bidens L. types were explored by methods of pharmacopeia. There were revealed essential differences of these types' morphological features and conformity of their anatomical structure.

scholarly journals Effect of Anatomical Structure of Shoots in Different Flowering Phase on Rhizogenesis of Once-blooming Roses

2017 ◽  
Vol 45 (2) ◽  
pp. 408-416 ◽  
Author(s):  
Marta J. MONDER ◽  
Paweł KOZAKIEWICZ ◽  
Agnieszka JANKOWSKA
Keyword(s):  
Adventitious Roots ◽  
Anatomical Structure ◽  
Flower Buds ◽  
Single Node ◽  
Rooted Cuttings ◽  
Anatomic Structure ◽  
Cambial Zone ◽  
Cambium Layer ◽  
Cambium Zone ◽  
Different Origin

The research was conducted on five once-blooming different origin historical roses. The aims were establishing differences in the anatomical structure of shoots taken in four maturity stages and their natural ability to roots creating. There were hypothesized, that the changes in anatomical structure occur in the time of flowering and may affect the ability of rhizogenesis. Single node one leaf cuttings of chosen cultivars were cut from shoots in four maturity phases: flower buds closed, all flowers open, just after and 7-14 days after petal fall. The cuttings were planted in multipot trays in peat substrate without using rooting stimulators. The anatomical structure of shoots in the place of rhizogenesis were observed, the width of xylem layer and cambial zone, diameter of vascular cells were measured. The percentage of rooted cuttings and forming only a callus were counted. Adventitious roots appeared probably in the cambium zone, pith rays, and also in the zone of phloem and pericycle. The anatomic structure of shoots changes during the period of flowering and influences rooting percentage and the percent of cuttings which developed callus only. The width of the cambium layer of shoots influences the width of the wood layer in all cultivars. The rise of width of cambium tissue have an effect of increase of percentage of cuttings forming a callus only in ‘Hurdals’ and ‘Maiden’s Blush’. The width of the cambium layer in shoots enlarges during full blooming in four of the studied cultivars (‘Hurdals’, ‘Maiden’s Blush’, ‘Mousseuse Rouge’, ‘Semiplena’) and in one it stays at the same level for the whole duration of the experiment (‘Duchesse d’Angoulême’). The specifying in practice the period of taking rose cuttings, individual properties of cultivars and phenological phase of shoot should be taken into consideration.

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