scholarly journals Antibacterial activity of different fractional extracts of cerastium glomeratum

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Himayat Ullah ◽  
Muhammad Hubaib ◽  
Muhammad Israr ◽  
Muhammad Mushtaq ◽  
Muhammad Zeeshan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Water Extract ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Active Components ◽  
Gram Positive ◽  
Gram Negative ◽  
Soxhlet Apparatus ◽  
Wide Range

Every plant available in the universe is not produced to be the part of biodiversity, but is also produced to obtain medicinal purposes. The medicinal value of the plant is due to the availability of various chemical compounds [1]. Since prehistoric time, the crude extract of plant is used to cure various diseases, although the biologically active components of the plant were unidentified [2]. In the present study the antibacterial activity is performed for various extracts of cerastium glomeratum. The plant cerastium glomeratum was collected from near kalpani river Mardan, Pakistan and was identified by lecturer Israr department of botany GPGC Mardan, Pakistan. The plant was dried under shade and grinded into powder form. The extraction process was done by using soxhlet apparatus, reflux condenser and maceration process using ethanol as a solvent. The extract was divided into different fractions of n-hexane, DCM, ethyl acetate and water. These four extracts were tested against gram positive (staphylococcus aureus) and gram negative (E.coli) bacteria using well diffusion technique. The water extract show a wide range inhibition zone against both gram positive and gram negative bacteria while n-hexane, DCM and ethyl acetate fractions show no zone of inhibition against bacteria. In effort to find new antibacterial compounds cerastium glomeratum seems to be a good plant for additional phytochemical studies.

scholarly journals Antibacterial activity of different fractional extracts of cerastium glomeratum

2020 ◽  
Vol 5 (2) ◽  
pp. 57-62
Author(s):  
Himayat Ullah ◽  
Muhammad Hubaib ◽  
Muhammad Israr ◽  
Muhammad Mushtaq ◽  
Muhammad Zeeshan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Water Extract ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Active Components ◽  
Gram Positive ◽  
Gram Negative ◽  
Soxhlet Apparatus ◽  
Wide Range

Every plant available in the universe is not produced to be the part of biodiversity, but is also produced to obtain medicinal purposes. The medicinal value of the plant is due to the availability of various chemical compounds [1]. Since prehistoric time, the crude extract of plant is used to cure various diseases, although the biologically active components of the plant were unidentified [2]. In the present study the antibacterial activity is performed for various extracts of cerastium glomeratum. The plant cerastium glomeratum was collected from near kalpani river Mardan, Pakistan and was identified by lecturer Israr department of botany GPGC Mardan, Pakistan. The plant was dried under shade and grinded into powder form. The extraction process was done by using soxhlet apparatus, reflux condenser and maceration process using ethanol as a solvent. The extract was divided into different fractions of n-hexane, DCM, ethyl acetate and water. These four extracts were tested against gram positive (staphylococcus aureus) and gram negative (E.coli) bacteria using well diffusion technique. The water extract show a wide range inhibition zone against both gram positive and gram negative bacteria while n-hexane, DCM and ethyl acetate fractions show no zone of inhibition against bacteria. In effort to find new antibacterial compounds cerastium glomeratum seems to be a good plant for additional phytochemical studies.

scholarly journals Antibacterial Activity and Flavonoids Content of Artemisia cina Berg. ex Poljakov Ethyl Acetate Extracts

2021 ◽  
Vol 13 (1) ◽  
pp. 106-112
Author(s):  
Sri Kasmiyati ◽  
Elizabeth Betty Elok Kristiani ◽  
Maria Marina Herawati ◽  
Andreas Binar Aji Sukmana
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Flavonoid Content ◽  
Gram Positive ◽  
Gram Negative ◽  
Significant Difference

The medicinal plant-derived bioactive compounds have a potential for many biological activities, including antimicrobial activity. Artemisia cina is a medicinal plant from the Compositae family with the potential of having antitumor, antifungal, and antibacterial activity. This study aimed to determine the antibacterial activity and the flavonoid content of A. Cina’s ethyl acetate extract. Plants samples were extracted by ethyl acetate maceration method. Antibacterial activity was tested against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) by a disk diffusion method using 25, 50, and 100 mg/l extract concentrations. The flavonoid contents (quercetin and kaempferol) were measured using High-Performance Liquid Chromatography. The extracts of diploid and polyploid A. cina displayed some antibacterial activity, with the Gram-negative bacteria being more resistant than the Gram-positive counterpart. However, no significant difference was observed between the diploid and polyploid extracts. As for the flavonoid content, the highest quercetin content (0.5501 mg/ml) was found in the polyploid A. cina (J), while the highest kaempferol content (0.5818 mg/ml) was observed in the diploid A. cina (KJT). Although A. cina is widely grown in Indonesia, compared to other Artemisia species, A. cina has not been widely studied, especially its antibacterial  potential and in related to its flavonoid content and the use of ethyl acetate as the extraction solvent.  This study reveals the potential of A. cina as a natural antibacterial agent. 

scholarly journals Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Yohannes Kelifa Emiru ◽  
Ebrahim Abdela Siraj ◽  
Tekleab Teka Teklehaimanot ◽  
Gedefaw Getnet Amare
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Maximum Activity ◽  
New Drugs ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

Objective. To evaluate the antibacterial effects of the leaf latex of Aloe weloensis against infectious bacterial strains. Methods. The leaf latex of A. weloensis at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as Escherichia coli (ATCC 14700) and Pseudomonas aeruginosa (ATCC 35619) and Gram-positive such as Staphylococcus aureus (ATCC 50080) and Enterococcus fecalis (ATCC 4623). Results. The tested concentrations of the latex ranging between 400 and 600 mg·mL−1 showed significant antibacterial activity against bacterial strain. The highest dose (600 mg/ml) of A. weloensis leaf latex revealed the maximum activity (25.93 ± 0.066 inhibition zone) followed by the dose 500 mg/ml against S. aureus. The lowest antibacterial activity was observed by the concentration 400 mg/ml (5.03 ± 0.03) against E. coli. Conclusion. The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections.

Antibacterial Activity of Amine-Functionalized Zeolite NaY against Staphylococcus aureus ATCC6538 and Escherichia coli ATCC11229

2015 ◽  
Vol 761 ◽  
pp. 402-406 ◽  
Author(s):  
Siti Aishah Mohd Hanim ◽  
Nik Ahmad Nizam Nik Malek ◽  
Zaharah Ibrahim ◽  
Mashitah Mad Salim ◽  
Nur Isti'anah Ramli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gram Positive ◽  
Gram Negative ◽  
Zeolite Nay ◽  
Amine Functionalized ◽  
Diffusion Technique

The antibacterial activity of functionalized zeolite NaY (CBV100) with different concentrations of 3-aminopropyltriethoxysilane (APTES) (0.01, 0.05, 0.20 and 0.40 M) was studied against Staphylococcus aureus ATCC 6538 (Gram positive) and Escherichia coli ATCC 11229 (Gram negative) through disc diffusion technique (DDT). The characterization of functionalized zeolite NaY with fourier transform infrared (FTIR) spectroscopy indicated the attachment of APTES on zeolite NaY. Through DDT, the inhibition zone of functionalized zeolite NaY increased proportionally to the amount of the amine-functional group attached onto zeolite NaY. Functionalized zeolite NaY showed higher antibacterial activity against Gram-positive compared to Gram-negative bacteria. It can be concluded from this study that amine-functionalized zeolite NaY shows evidence of antibacterial activities.

scholarly journals Quercetin as a Precursor for the Synthesis of Novel Nanoscale Cu (II) Complex as a Catalyst for Alcohol Oxidation with High Antibacterial Activity

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Zahra Moodi ◽  
Ghodsieh Bagherzade ◽  
Janny Peters
Keyword(s):  
Antibacterial Activity ◽  
Schiff Base ◽  
Biological Activities ◽  
Schiff Base Complex ◽  
13C Nmr Spectroscopy ◽  
Inhibition Zone ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
High Antibacterial Activity

Quercetin (3,3′,4′,5,7-pentahydroxyflavone) is one of the dietary flavonoids, distributed in medicinal plants, vegetables, and fruits. Quercetin has the ability to bind with several metal ions to increase its biological activities. In the last two decades, quercetin has attracted considerable attention due to the biological and pharmaceutical activities such as antioxidant, antibacterial, and anticancer. In the present study, quercetin and ethanolamine were used for the synthesis Schiff base complex, which was characterized by IR, 1H NMR, and 13C NMR spectroscopy. The Schiff base has been employed as a ligand for the synthesis of novel nanoscale Cu (II) complex. The product was characterized by FT-IR spectroscopy, FESEM, and XRD. Significantly, the product showed remarkable catalytic activity towards the oxidation of primary and secondary alcohols. The antibacterial activity of the final product was assessed against Staphylococcus aureus (Gram‐positive) and Escherichia coli (Gram‐negative) bacteria using an inhibition zone test. The synthesized nanoscale Cu (II) complex exhibited a strong antibacterial activity against both Gram-positive and Gram-negative bacteria.

scholarly journals Molecular Computation and Antibacterial Activity of Cu (II) Complex of Naphthaldehyde Thiosemicarbazone

2020 ◽  
pp. 85-93
Keyword(s):  
Antibacterial Activity ◽  
Molecular Geometry ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Spectral Measurements ◽  
Gram Positive ◽  
Activity Data ◽  
Gram Negative ◽  
Diffusion Technique ◽  
Parent Ligand

Copper (II) complex of naphthaldehyde thiosemicarbazone (L) has been synthesized and characterized by melting points, conductance, magnetic, infrared, and ESI-MS spectral measurements in addition to elemental analysis. A tetrahedral structure is suggested for the complex. The antibacterial activities of the complex and ligand were evaluated by the disc diffusion technique. Pure bacteria cultures of Bacillus subtilis (Gram-positive) and Escherichia coli (Gram-negative) were used to check the antibacterial activities of the synthesized compounds. Antibacterial activities were compared by measuring the inhibition zone diameter and chloramphenicol was used as a reference. Both the compounds showed significant antibacterial activity in different range against gram-positive & gram-negative bacteria. The antibacterial activity data also show that the Cu(II) complex to be more effective than the parent ligand. Molecular geometry of the complex has been optimized by ChemDraw Ultra 12.0 and then MM2 calculation has been done.

scholarly journals Pyoverdines are essential for the antibacterial activity of Pseudomonas chlororaphis YL-1 under low-iron conditions

2021 ◽  
Author(s):  
Youzhou Liu ◽  
Chen Dai ◽  
Yaqiu Zhou ◽  
Junqing Qiao ◽  
Bao Tang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Sufficient Conditions ◽  
Antibacterial Activities ◽  
Pseudomonas Chlororaphis ◽  
Iron Starvation ◽  
Gram Positive ◽  
Biosynthesis Gene Cluster ◽  
Gram Negative ◽  
Wide Range

Pseudomonas chlororaphis YL-1 has extensive antimicrobial activities against phytopathogens, and its genome harbors pyoverdine (PVD) biosynthesis gene cluster. The alternative sigma factor PvdS in Pseudomonas aeruginosa PAO1, acts as a critical regulator in response to iron starvation. The assembly of the PVD backbone starts with peptide synthetase enzyme PvdL. PvdF catalyzes formylation of L-OH-Orn to produce L-N5-hydroxyornithine. Here, we describe the characterization of PVD production in YL-1 and its antimicrobial activity as compared with its PVD-deficient mutants ΔpvdS, ΔpvdF, and ΔpvdL, that were obtained using a sacB-based site-specific mutagenesis strategy. Using in vitro methods, we examined the effect of exogenous iron under low-iron conditions and iron-chelating agent under iron-sufficient conditions on PVD production, antibacterial activity, and the relative expression of PVD transcription factor gene pvdS in YL-1. We found that strain YL-1, mutant ΔpvdF, and complemented strain ΔpvdS(pUCP26-pvdS) produced visible PVDs and demonstrated a wide range of inhibitory effects against Gram-negative and Gram-positive bacteria in vitro under low-iron conditions, and that with the increase of iron its PVD production and antibacterial activity were reduced. The antibacterial compounds produced by strain YL-1 in low-iron conditions were PVDs based on the liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Moreover, the antibacterial activity observed in vitro was correlated with in vivo control efficacies of strain YL-1 against rice bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae （Xoo）. Collectively, PVDs are responsible for the antibacterial activities of strain YL-1 under both natural and induced low-iron conditions. IMPORTANCE: The results demonstrated that PVDs are essential for the broad-spectrum antibacterial activities of strain YL-1 against both Gram-positive and Gram-negative bacteria in low-iron conditions. Our findings also highlight the effect of exogenous iron on the production of PVD and the importance of this bacterial product in bacterial interactions. As a biocontrol agent, PVDs can directly inhibit the proliferation of the tested bacteria in addition to participating in iron competition.

scholarly journals Antibacterial Activity of Floral Petals of Some Angiosperms

2019 ◽  
pp. 1-5 ◽  
Author(s):  
L. Rajanna ◽  
N. Santhosh Kumar ◽  
N. S. Suresha ◽  
S. Lavanya
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Bacterial Strains ◽  
Inhibitory Zone ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Antibacterial Assay

The in vitro antibacterial assay was carried out against both Gram positive (B. cerus and S. aureus) and Gram negative (E. coli and K. pneumoniae) bacteria. Floral petals of 20 different species of plants were collected and tested for antibacterial activity. The result showed that the petals were active against both Gram positive and Gram negative. Out of 20 floral petals tested, 19 floral petals exhibited antibacterial activity against selected bacterial strains. The minimal inhibitory zone of floral petal discs against human pathogenic bacteria varies from 2 – 6 mm. Rosa carolina and Ruellia tuberosa showed significance inhibition zone for all the bacterial strains while Lantana camara does not show inhibition zone for any of these pathogenic bacteria.

scholarly journals LC-MS/MS profiling, antibiofilm, antimicrobial and bacterial growth kinetic studies of Pluchea dioscoridis extracts

2021 ◽  
Author(s):  
Maha A.M. El-Shazly ◽  
Ahmed A. Hamed ◽  
Hoda A. Kabary ◽  
Mosad A. Ghareeb
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Incubation Period ◽  
Phenolic Acids ◽  
Ethyl Acetate Extract ◽  
Wide Spectrum ◽  
Water Extract ◽  
Inhibition Zone ◽  
Dependent Manner ◽  
Prolonged Incubation

Abstract The therapeutical applications of ornamental plants have been categorized to be of a great effectiveness in multiple industries from ancient times until present days. Pluchea dioscoridis is widely known Egyptian wooden plant that has been extensively applied for different medicinal purposes. In this study, LC-ESI-MS/MS analysis of the potent antimicrobial ethyl acetate and n-butanol extracts of P. dioscoridis leaves led to identification of 28 and 21 compounds, respectively. The identified compounds were categorized as phenolic acids, phenolic acids derivatives, organic acids, flavonoids (aglycones and glycosides), secoiridoids, coumarin derivatives, and gallotannins derivatives. Among them, caffeic acid 3-sulfate was the most predominate in the investigated extracts followed by ferulic acid and dicaffeoyl-quinic acid. Also, the antimicrobial potentiality of different extracts was evaluated against different pathogenic microbes including Enterobacter cloacae, Micrococcus leutus, Aeromonas hydrophila, Bacillus subtilis, Bacillus cereus, Bacillus lichneformis and Clostridium species. Furthermore, different concentrations of the most potent extract were assayed for antibacterial efficacy on growth curve kinetics against the susceptible bacteria along 4days incubation period. Our gathered data confirmed that, the antimicrobial activity against tested bacteria was different according to the solvent used in the extraction process. Mostly, all the extracts showed a wide spectrum antibacterial activity except the plant water extract which shows a mild activity against Clostridium sp. only. Based on the highest inhibition zone diameter, the ethyl acetate extract followed by butanol extract exhibited the highest inhibition zone with Micrococcus luteus and B. subtilis (20.0 and 18.5 mm) respectively. Determining the effect of ethyl acetate extract at different concentration (0, 0.66, 1.66, 3.33, 6.67, 13.34 and 20.01 mg mL−1) on M. luteus growth kinetics, the data assured that the antibacterial activity shows concentration dependent manner with the highest antibacterial activity at 20.01 mg mL−1 culture. The data also confirmed that, none of the selected concentration showed bactericidal activity in the prepared cultures, and with the prolonged incubation period the bacteria acquire resistance against the extract beginning from second or third day of incubation.

scholarly journals DETERMINATION OF ANTIBACTERIAL ACTIVITY OF SOME IMPORTANT SPICES

2015 ◽  
Vol 3 (10) ◽  
pp. 57-64
Author(s):  
Ranganathan Kapilan
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Maximum Activity ◽  
Gram Negative Bacteria ◽  
Alcoholic Extract ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Wide Range

Wide range of plant extracts are used for medicinal purposes as they are very cheap, efficient, harmless and do not cause any side effects. Spices are parts of different plants and they add special aroma and taste to the food preparations. The aim of the study was to determine the antimicrobial activity of some important naturally grown spices against gram positive and gram negative pathogenic bacteria. Antibacterial activity of the spices was tested against gram positive bacteria Bacillus pumilus, Bacillus cereus and Staphylococcus aureus and gram negative bacteria Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa using aqueous, ethanolic, methanolic and liquid nutrient extracts. Among all the extracts tested alcoholic extracts of Cardamom (Elettaria cardamom), clove (Eugenia caryophyllus) and lemongrass (Cymbopogoncitratus) showed maximum antimicrobial activity against gram negative bacteria while alcoholic extract of Cardamom (Elettaria cardamom) and lemongrass (Cymbopogoncitratus) showed maximum activity against gram positive bacteria. All the spices tested in this study proved that they have antibacterial activity and the maximum activity index (1.39) was exhibited by the ethanol extract of cardamom against E.coli.

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