scholarly journals LC-MS/MS profiling, antibiofilm, antimicrobial and bacterial growth kinetic studies of Pluchea dioscoridis extracts

2021 ◽  
Author(s):  
Maha A.M. El-Shazly ◽  
Ahmed A. Hamed ◽  
Hoda A. Kabary ◽  
Mosad A. Ghareeb
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Incubation Period ◽  
Phenolic Acids ◽  
Ethyl Acetate Extract ◽  
Wide Spectrum ◽  
Water Extract ◽  
Inhibition Zone ◽  
Dependent Manner ◽  
Prolonged Incubation

Abstract The therapeutical applications of ornamental plants have been categorized to be of a great effectiveness in multiple industries from ancient times until present days. Pluchea dioscoridis is widely known Egyptian wooden plant that has been extensively applied for different medicinal purposes. In this study, LC-ESI-MS/MS analysis of the potent antimicrobial ethyl acetate and n-butanol extracts of P. dioscoridis leaves led to identification of 28 and 21 compounds, respectively. The identified compounds were categorized as phenolic acids, phenolic acids derivatives, organic acids, flavonoids (aglycones and glycosides), secoiridoids, coumarin derivatives, and gallotannins derivatives. Among them, caffeic acid 3-sulfate was the most predominate in the investigated extracts followed by ferulic acid and dicaffeoyl-quinic acid. Also, the antimicrobial potentiality of different extracts was evaluated against different pathogenic microbes including Enterobacter cloacae, Micrococcus leutus, Aeromonas hydrophila, Bacillus subtilis, Bacillus cereus, Bacillus lichneformis and Clostridium species. Furthermore, different concentrations of the most potent extract were assayed for antibacterial efficacy on growth curve kinetics against the susceptible bacteria along 4days incubation period. Our gathered data confirmed that, the antimicrobial activity against tested bacteria was different according to the solvent used in the extraction process. Mostly, all the extracts showed a wide spectrum antibacterial activity except the plant water extract which shows a mild activity against Clostridium sp. only. Based on the highest inhibition zone diameter, the ethyl acetate extract followed by butanol extract exhibited the highest inhibition zone with Micrococcus luteus and B. subtilis (20.0 and 18.5 mm) respectively. Determining the effect of ethyl acetate extract at different concentration (0, 0.66, 1.66, 3.33, 6.67, 13.34 and 20.01 mg mL−1) on M. luteus growth kinetics, the data assured that the antibacterial activity shows concentration dependent manner with the highest antibacterial activity at 20.01 mg mL−1 culture. The data also confirmed that, none of the selected concentration showed bactericidal activity in the prepared cultures, and with the prolonged incubation period the bacteria acquire resistance against the extract beginning from second or third day of incubation.

scholarly journals Antibacterial Activities of Endophytic Fungi Isolated from Mentha cordifolia Leaves and Their Volatile Constituents

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Sakon Monggoot ◽  
Jariya Burawat ◽  
Patcharee Pripdeevech
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
High Concentration ◽  
Phenyl Ethyl Alcohol ◽  
First Time

A total of 17 endophytic fungal isolates were obtained from the leaves of Mentha cordifolia Opiz (Lamiaceae). Seven isolates were identified to the level of genus by using taxonomically relevant morphological traits. Colletotrichum and Phomopsis species were dominant among these strains. All strains were separated from M. cordifolia leaf for the first time. The ethyl acetate extracts of all endophytic fungi were tested for antibacterial activity against Salmonella typhimurium TISTR1166 and Pseudomonas aeruginosa TISTR781. Most endophytes exhibited antibacterial activity. Ustilago sp. MFLUCC15-1024 presented the highest inhibition zone diameter with a MIC of 31.25 μg/mL against the tested pathogens. The chemical composition of the ethyl acetate extract of this strain was investigated using gas chromatography-mass spectrometry. Twenty-one components were identified. 2-Phenylethanol (38.7%), E-ligustilide (12.4%), α-eudesmol (10.2%), β-vetivone (4.6%), β-ylangene (3.7%) and verbanol (3.4%) were the major components of the extract. The strong antibacterial activity of Ustilago sp. MFLUCC15-1024 ethyl acetate extract may be attributed to the presence of a high concentration of bioactive compounds including phenyl ethyl alcohol, E-ligustilide and α-eudesmol. The results indicate that there is high diversity of endophytic fungi in M. cordifolia leaf, and that Ustilago sp. MFLUCC15-1024 strain could be an excellent resource of natural antibacterial compounds.

scholarly journals Bioactive Compounds Isolated from Indonesian Epiphytic Plant of Sarang Semut and Their Antibacterial Activity against Pathogenic Oral Bacteria

2017 ◽  
Vol 12 (8) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Dikdik Kurnia ◽  
Dadan Sumiarsa ◽  
Hendra D.A. Dharsono ◽  
Mieke H. Satari
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Porphyromonas Gingivalis ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Steroid Glycoside ◽  
Oral Bacteria ◽  
Epiphytic Plant

A new phenolic compound (1), a steroid (2), a new steroid glycoside (4), two triterpenoids (3 & 6) and a new phloroglucinol-sesquiterpene (5) have been isolated from the ethyl acetate extract of Sarang Semut ( Myrmecodia pendans) and their structures were determined on the basis of the spectral data. The bioactivity evaluation was conducted with the inhibition zone of compounds (mm) using Kirby-Bauer method at concentrations of 1000 and 5000 ppm for compound 1 against pathogenic oral bacteria Enterococcus faecalis, was 8.55 and 8.05 mm, respectively. Compounds 2–3 against Streptococcus mutans were 9.00 and 8.45 mm (2) and 10.24 and 9.35 mm (3), respectively. Compound 5 against Porphyromonas gingivalis was 11.5 and 10.8 mm, respectively.

scholarly journals Antibacterial activity of endosymbiotic fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands, Jakarta

2021 ◽  
Vol 948 (1) ◽  
pp. 012069
Author(s):  
R Trifani ◽  
Noverita ◽  
T A Hadi ◽  
E Sinaga
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Marine Sponges ◽  
Disc Diffusion ◽  
Active Compounds ◽  
E Coli ◽  
Stylissa Carteri ◽  
Further Development

Abstract Endosymbiont fungi from marine sponges are a rich source of medicinally active compounds. Indonesia has a huge number of marine sponges. This research was conducted to determine the antibacterial potential of endosymbiont fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands. The screening was conducted with two methods, the modified GIBEX and disc diffusion. Species of marine sponges were isolated, namely Petrosia sp., Stylissa carteri, Cinachyrella australiensis, Callyspongia sp., Petrosia nigrians, and Stylissa massa, and obtained 9 isolates of endosymbiont fungi. The GIBEX test against Escherichia coli and Streptococcus mutans showed that ethyl acetate extract had strongest antibacterial activity. Disc diffusion test of ethyl acetate extract, five isolates had antibacterial activity against S. mutans and four against E. coli. The isolate that showed the strongest antibacterial activity was from Petrosia sp. Which has an inhibition zone of 8.4 mm against E. coli and 7.45 mm against S. mutans. The main active compounds from the isolate of Petrosia sp. are butylhydroxytoluene and phthalic acid di-(2-propylpentyl) esters. Based on this study, we concluded that the endosymbiont fungi of marine sponges are potential to be developed for further development as source of antibacterial agents.

scholarly journals UJI EFEKTIVITAS DAUN PANDAN WANGI (PANDANUS AMARYLLIFOLIUS ROXB.) SEBAGAI ANTIBAKTERI TERHADAP SALMONELLA TYPHI

2019 ◽  
Vol 6 (1) ◽  
pp. 59
Author(s):  
Priska Nancy Claudia Bali ◽  
Ahmad Raif ◽  
Setia Budi Tarigan
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Inhibition Zone Diameter ◽  
Negative Controls ◽  
Pandanus Amaryllifolius ◽  
Positive Controls

Pandanus amaryllifolius roxb leaves commonly used as food dditives which contain various compound groups namely alkaloid, saponins, flavonoids and tannins that have a role in antibacterial activity. This research is indicated to support the success of antibacterial in fragrant pandanus leaf plants against Salmonella typhi, one of the pathogenic bacteria. The antibacterial effectiveness test of ethanol-ethyl acetate extract (1: 1) of pandanus leaves which evaluated by the Kirby-bauer method and making the extract concentrations of 25%, 50%, 75%, 100%. The results showed that pandanus leaves extract had antibacterial effectiveness against Salmonella typhi with an average inhibition zone diameter of 11.6 mm; 14 mm; 14.3 mm; 15.3 mm; positive controls (Ciprofloxacin) 27 mm; and negative controls (aquadest) didn’t produce the inhibition zone of Salmonella typhi.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TENGGULUN (Protium javanicum Burm. F.) TERHADAP BAKTERI Staphylococcus aureus

Jurnal Kimia ◽  
2020 ◽  
pp. 56
Author(s):  
N. M. Puspawati ◽  
N. L. P. F. Widiari ◽  
I M. Sukadana
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Spectra Analysis ◽  
Minimum Concentration ◽  
Butanol Extract ◽  
Leaf Powder ◽  
Inhibition Zones

This study aims to examine the antibacterial activity of n-hexane, ethyl acetate, and n-butanol extract of tenggulun leaves (Protium javanicum Burm. F.) in inhibiting the growth of S. aureus bacteria and determine the minimum inhibitory concentration of the most active extracts and identify its active compounds. A total of 1000 g of tenggulun leaf powder was macerated with methanol and 91.78 g of crude methanol extract was obtained which was further partitioned into n-hexane, ethyl acetate, and n-butanol extracts. Antibacterial activity test was carried out using the diffusion well method and the result showed that the ethyl acetate extract at a concentration of 20% was strongly able to inhibit the growth of S. aureus bacteria with inhibition zones of 20.08 mm, followed by n-butanol 16.66 mm, and n-hexane 13.33 mm. The minimum concentration of ethyl acetate extract was 0.2% in inhibiting the growth of S. aureus with inhibition zone diameter of 6.20 mm. Separation of the active ethyl acetate extract was conducted by silica gel column chromatography with mobile phase of n-hexane: ethyl acetate (7.5: 2.5) gave 6 combined fractions. Fractions B, G, H and I showed antibacterial activity at concentrations of 50% with a diameter of inhibition zone of 5.62 mm, 6.87 mm, 8.50 mm, and 6.75 mm respectively. The results of mass spectra analysis from the chromatogram peaks of LC-MS / MS suggested that the G and I fractions were tentatively identified as crotarin, benzophenone-2, and medroxyprogesterone acetate which may contributed to the antibacterial activity by acting synergisticly.    Keywords: antibacterial, tenggulun, Staphylococcus aureus, LC-MS/MS.

scholarly journals Antibacterial activity of different fractional extracts of cerastium glomeratum

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Himayat Ullah ◽  
Muhammad Hubaib ◽  
Muhammad Israr ◽  
Muhammad Mushtaq ◽  
Muhammad Zeeshan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Water Extract ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Active Components ◽  
Gram Positive ◽  
Gram Negative ◽  
Soxhlet Apparatus ◽  
Wide Range

Every plant available in the universe is not produced to be the part of biodiversity, but is also produced to obtain medicinal purposes. The medicinal value of the plant is due to the availability of various chemical compounds [1]. Since prehistoric time, the crude extract of plant is used to cure various diseases, although the biologically active components of the plant were unidentified [2]. In the present study the antibacterial activity is performed for various extracts of cerastium glomeratum. The plant cerastium glomeratum was collected from near kalpani river Mardan, Pakistan and was identified by lecturer Israr department of botany GPGC Mardan, Pakistan. The plant was dried under shade and grinded into powder form. The extraction process was done by using soxhlet apparatus, reflux condenser and maceration process using ethanol as a solvent. The extract was divided into different fractions of n-hexane, DCM, ethyl acetate and water. These four extracts were tested against gram positive (staphylococcus aureus) and gram negative (E.coli) bacteria using well diffusion technique. The water extract show a wide range inhibition zone against both gram positive and gram negative bacteria while n-hexane, DCM and ethyl acetate fractions show no zone of inhibition against bacteria. In effort to find new antibacterial compounds cerastium glomeratum seems to be a good plant for additional phytochemical studies.

scholarly journals Uji Aktivitas Sitotoksik dan Antibakteri Ekstrak Daun Tumbuhan Rengas (Gluta renghas L)

2020 ◽  
Vol 11 (1) ◽  
pp. 52-60
Author(s):  
Sanusi Ibrahim ◽  
Suryati Suryati ◽  
Enda Desriansyah Aziz
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Cytotoxicity Activity ◽  
E Coli ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of ◽  
Ethyl Acetate Extracts

Generally, Gluta renghas L. is known because of its very toxic latex which can cause hard irritations to skin. Although, rengas latex has efficacy as an antibacterial agent. Related to previous research, it has been reported that there are urisol, rengol, glutarengol, laccol, and thitsiol in rengas latex. The woody trunk was reported to contain flavonoid, benzenoid, lipid, and steroid compounds. The leaves, were reported shows that the leaves of G. renghas contain an anticholinesterase substance and can be reduced the rate of hydrolysis of acetylcholine. Extraction of rengas leaves has been carried out. It has been shown phenols, steroids, and coumarins compounds in methanol extract, flavonoids, phenols, saponins and alkaloids compounds in ethyl acetate extract, steroids and alkaloids compounds in hexane extract. In the present, cytotoxicity and antibacterial activity have been tested. The results show that the great cytotoxicity activity by ethyl acetate extracts which have an LC50 value is 123,718 µg/mL (R2 0.9822), while the great antibacterial activity shown by methanol extract 1,000 µg/mL with a diameter of inhibition zone 19.02 mm (S. aureus) and 16.06 mm (E. coli). 

scholarly journals Antibacterial activity of Piper betle extracts on Helicobacter pylori and identification of potential compounds

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Nguyen Thi Huyen Trang ◽  
Do Thi Thanh Trung ◽  
Nguyen Thi Thanh Thi ◽  
Pham Thi Luong Hang ◽  
Pham Thi Vinh Hoa ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Piper Betle ◽  
Gastric Diseases ◽  
Disk Diffusion Assay ◽  
H Pylori

Helicobacter pylori is one of the most common infectious bacteria in the world that causes gastric diseases leading to cancer. The increase of multiple antibiotic resistance rates of H. pylori have been reported worldwide. Thus, development of novel drugs is urgently required. Piper betle has many therapeutic values in traditional medicine. In this study, therefore, we investigated antibacterial activity of P. betle extracts and their fractions against a H. pylori strain isolated in Vietnam. The agar disk diffusion assay showed inhibition zone of ethyl acetate extract and methanol extract from P. betle leaf that of were 46 mm and 32 mm in diameter, respectively. After fractionation of the ethyl acetate extract through silica gel column chromatography, two peaks, PD2 and PD3, out of 12 fractions showed the strongest antibacterial activity. PD2 was sub-fractionated further by re-chromatography on the silica gel column, and subfraction TK12 gave best resolution on LC-MS analysis. Finally, 4 potential compounds, quercetrin, calodenin B, vitexin and plicatipyrone, were identified in TK12 fraction. 

scholarly journals Antibacterial activity of Piper betle extracts on Helicobacter pylori and identification of potential compounds

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Nguyen Thi Huyen Trang ◽  
Do Thi Thanh Trung ◽  
Nguyen Thi Thanh Thi ◽  
Pham Thi Luong Hang ◽  
Pham Thi Vinh Hoa ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Silica Gel ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Piper Betle ◽  
Gastric Diseases ◽  
Disk Diffusion Assay ◽  
H Pylori

Helicobacter pylori is one of the most common infectious bacteria in the world that causes gastric diseases leading to cancer. The increase of multiple antibiotic resistance rates of H. pylori have been reported worldwide. Thus, development of novel drugs is urgently required. Piper betle has many therapeutic values in traditional medicine. In this study, therefore, we investigated antibacterial activity of P. betle extracts and their fractions against a H. pylori strain isolated in Vietnam. The agar disk diffusion assay showed inhibition zone of ethyl acetate extract and methanol extract from P. betle leaf that of were 46 mm and 32 mm in diameter, respectively. After fractionation of the ethyl acetate extract through silica gel column chromatography, two peaks, PD2 and PD3, out of 12 fractions showed the strongest antibacterial activity. PD2 was sub-fractionated further by re-chromatography on the silica gel column, and subfraction TK12 gave best resolution on LC-MS analysis. Finally, 4 potential compounds, quercetrin, calodenin B, vitexin and plicatipyrone, were identified in TK12 fraction. 

Effect of Chroogomphis Rutillus Extracts on Activity of Nitrite Scavenging and Nitrosamine Formation Inhibition

2011 ◽  
Vol 282-283 ◽  
pp. 545-548
Author(s):  
Hong Fang Ji ◽  
Ling Wen Zhang ◽  
Hui Hui Jin ◽  
Ling Yu Wang ◽  
Fang Yuan Cheng ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Water Extract ◽  
Dependent Manner ◽  
In Vitro Systems ◽  
Dose Dependent ◽  
Ethyl Acetate Extracts ◽  
Scavenging Rates

Chroogomphis rutillusextracts were prepared with solvent of petroleum ether, acetic ester, methanol and water. The effects of four different extract on nitrite scavenging (NS) and nitrosamine formation inhibiting (NFI) were evaluated in vitro systems. Results showed that all the extract showed NS and NFI potentials with a dose-dependent manner. With regard to NS activity, at 50μg/mL, the scavenging rates of ethyl acetate and methanol extracts were 96.65% and 93.48%, respectively. While, those of petroleum ether and water extract were only 86.96% and 26.09%, respectively. With regard to NFI activity,at 200μg/mL, both petroleum ether and ethyl acetate extracts showed excellent inhibition abilities of 94.46% and 91.33%, respectively, much higher than those of methanol and water extract (78.57% and 4.06%, respectively) (P<0.05).Chroogomphis rutillusespecially ethyl acetate extract may have a preventive effect against carcinogenesis induced by nitrosamines.

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