scholarly journals Study on the Relationship between Development of Periodontal Disease and Cleavage of Complement : Measurements of C3 in Rat Serum and Gingival Extracts by Enzyme Linked Immunosorbent Assay

1986 ◽  
Vol 40 (4) ◽  
pp. 836-844
Author(s):  
Naritoshi Matsubara ◽  
Hiromasa Inoue ◽  
Kensuke Haga ◽  
Choji Uchiyama ◽  
Hakubu Yamamoto
Keyword(s):  
Periodontal Disease ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rat Serum ◽  
The Relationship

scholarly journals HUBUNGAN ANTARA INTENSITAS ESTRUS DENGAN KONSENTRASI ESTRADIOL PADA SAPI ACEH PADA SAAT INSEMINASI (Relation between Estrous Intensity and Estradiol Concentration on Local Cattle during Insemination)

2016 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Mauridatun Ramli ◽  
Tongku Nizwan Siregar ◽  
Cut Nila Thasmi ◽  
Dasrul Dasrul ◽  
Sri Wahyuni ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Jugular Vein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasma Samples ◽  
Hormone Concentration ◽  
Prostaglandin F2 Alpha ◽  
Commercial Kits ◽  
The Relationship ◽  
Estradiol Concentration

This study aims to determine the relationship between the intensity of estrus and estradiol concentrations during insemination on local cattle. Ten cows were divided into two groups with each group consist of five cows with category: aged 5-8 years, weight 150-250 kg, clinically healthy, and not pregnant. Plasma samples for examination of the hormone estradiol concentrations were collected during insemination time from jugular vein. Prior to the collection of plasma samples, whole cow were estrus synchronized using prostaglandin F2 alpha (PGF2α) and Ovsynch protocol. The PGF2α were injected twice at intervals of 11 days. Examination of estradiol hormone concentration was performed with enzyme-linked immunosorbent assay (ELISA) using estradiol commercial kits. The results of this study indicate that statistically there is no relationship between estrous intensity and estradiol concentration.

scholarly journals Effect of Anti-TNF Antibodies on Clinical Response in Rheumatoid Arthritis Patients: A Meta-Analysis

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Chunxiao Wu ◽  
Shengxu Wang ◽  
Peifeng Xian ◽  
Lu Yang ◽  
Ying Chen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Clinical Response ◽  
Immunosorbent Assay ◽  
Meta Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tnf Inhibitor ◽  
Chip Technology ◽  
Assay Methods ◽  
The Relationship

Background. Antitumor necrosis factor (anti-TNF) drugs have been applied for rheumatoid arthritis (RA) treatment; however, patients having anti-drug antibodies (ADAbs) do not benefit from these drugs. The meta-analysis aims to comprehensively assess the relationship between ADAb positive (ADAb+) and anti-TNF response in RA patients.Methods. Observational studies comparing different clinical response between ADAb+ and ADAb negative groups were included. Odds ratio (OR) with its corresponding 95% confidence interval (CI) was used as effect size. Subgroup analyses stratified by TNF inhibitor types and assay methods for ADAb detection were performed.Results. Totally, 10 eligible studies containing 1806 subjects were included. ADAb+ was significantly associated with reduced anti-TNF response to RA at all the time points after follow-up (P<0.001). Subgroup analysis also supported this significant association (P<0.05), except for enzyme-linked immunosorbent assay (ELISA) group at 3 months, infliximab (INF) and enzyme-linked immunosorbent assay (ELISA) groups at 6 months, and Immunological Multi-Parameter Chip Technology (IMPACT) group at 12 months.Conclusion. ADAb+ was significantly associated with reduced clinical response in RA patients, and other alternatives should be considered in RA patients presenting ADAb+.

scholarly journals Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review

2016 ◽  
Vol 2016 ◽  
pp. 1-23 ◽  
Author(s):  
Zeyad Nazar Majeed ◽  
Koshy Philip ◽  
A. M. Alabsi ◽  
Saravanan Pushparajan ◽  
Dasan Swaminathan
Keyword(s):  
Periodontal Disease ◽  
Immunosorbent Assay ◽  
Analytical Methods ◽  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Fluid Collection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Precise Method ◽  
Biomarker Analysis ◽  
Crevicular Fluid

Background. Several studies in the last decades have focused on finding a precise method for the diagnosis of periodontal disease in its early stages.Aim. To evaluate from current scientific literature the most common and precise method for gingival crevicular fluid (GCF) sample collection, biomarker analytical methods, and the variability of biomarker quantification, even when using the same analytical technique.Methodology. An electronic search was conducted on in vivo studies that presented clinical data on techniques used for GCF collection and biomarker analysis.Results. The results showed that 71.1%, 24.7%, and 4.1% of the studies used absorption, microcapillary, and washing techniques, respectively, in their gingival crevicular fluid collection. 73.1% of the researchers analyzed their samples by using enzyme-linked immunosorbent assay (ELISA). 22.6%, 19.5%, and 18.5% of the researchers included interleukin-1 beta (IL-1β), matrix metalloproteinase-8 (MMP-8), and tumor necrosis factor-alpha (TNF-α), respectively, in their studies as biomarkers for periodontal disease.Conclusion. IL-1βcan be considered among the most common biomarkers that give precise results and can be used as an indicator of periodontal disease progression. Furthermore, paper strips are the most convenient and accurate method for gingival crevicular fluid collection, while enzyme-linked immunosorbent assay can be considered the most conventional method for the diagnosis of biofluids.

DEVELOPMENT OF AN ANTIGEN HETEROLOGOUS ENZYME LINKED IMMUNOSORBENT ASSAY FOR MEASUREMENT OF CORTICOSTERONE IN RAT SERUM

2011 ◽  
Vol 32 (3) ◽  
pp. 244-257 ◽  
Author(s):  
Tulsidas G. Shrivastav ◽  
Shail K. Chaube ◽  
Kiran P. Kariya ◽  
Kiran Rangari ◽  
Rita Singh
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rat Serum

Detection of domoic acid in rat serum and brain by direct competitive enzyme-linked immunosorbent assay (cELISA)

2005 ◽  
Vol 383 (5) ◽  
pp. 783-786 ◽  
Author(s):  
Blair R. Hesp ◽  
Joanne C. Harrison ◽  
Andrew I. Selwood ◽  
Patrick T. Holland ◽  
D. Steven Kerr
Keyword(s):  
Immunosorbent Assay ◽  
Domoic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rat Serum

scholarly journals Characteristics of measles immunity in students of the pediatric faculty of a medical university

2020 ◽  
Vol 12 (4) ◽  
pp. 58-64
Author(s):  
D. O. Ivanov ◽  
V. N. Timchenko ◽  
E. B. Pavlova ◽  
E. V. Barakina ◽  
T. M. Chernova ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Measles Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Measles Vaccine ◽  
Specific Antibodies ◽  
Measles Antibodies ◽  
Measles Vaccination ◽  
Instructions For Use ◽  
Medical University ◽  
The Relationship

Objective: to evaluate the intensity of measles immunity in students of I–VI courses of the pediatric faculty of St. Petersburg State Medical University.Materials and methods: titers of antibodies to measles virus were determined in 986 students of I–VI courses in May –June 2019. The level of measles antibodies was determined by enzyme-linked immunosorbent assay.Results: positive (protective) titres of measles antibodies were detected in 555 people. (56.4%), the absence of measles immunity was found in 431 people. (43.6%), including 57 people. (5.8%) specific antibodies were not detected. The relationship between the level of collective immunity and the duration of the period after the last vaccination against measles was revealed.Conclusions: in the majority of examined students (96.2%), protective titers of measles antibodies remained for 10 years after the last vaccination. Subsequently, a significant decrease in the level of measles antibodies was noted. All students who do not have a protective IgG titer are shown measles vaccination with live measles vaccine in accordance with the instructions for use. 

scholarly journals Differences in Immune Response to Porphyromonas gingivalis

2014 ◽  
Vol 15 (5) ◽  
pp. 573-575 ◽  
Author(s):  
Konstantin G Gurevich ◽  
Laura M Tebloeva ◽  
Zalina E Revazova ◽  
Kate G Fabrikant ◽  
Lidia A Dmitrieva
Keyword(s):  
Immune Response ◽  
Periodontal Disease ◽  
Blood Serum ◽  
Porphyromonas Gingivalis ◽  
Immunosorbent Assay ◽  
Hormonal Therapy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Effective Immune Response

ABSTRACT About 341 (average age of 38.0 ± 2.5 years) patients with periodontal disease were included in this study. All the patients were not treated for periodontal disease for a period of 6 months prior to this study. All the patients were not on any hormonal therapy. Antibodies to Porphyromonas gingivalis (P. gingivalis) were determined in blood serum by enzyme-linked immunosorbent assay (ELISA) method. Findings from this study suggest immune response to P. gingivalis is more effective in males than in females. Smoking and obesity tends to reduce effective immune response. How to cite this article Tebloeva LM, Revazova ZE, Fabrikant KG, Dmitrieva LA, Gurevich KG. Differences in Immune Response to Porphyromonas gingivalis. J Contemp Dent Pract 2014;15(5):573-575.

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