scholarly journals HUBUNGAN ANTARA INTENSITAS ESTRUS DENGAN KONSENTRASI ESTRADIOL PADA SAPI ACEH PADA SAAT INSEMINASI (Relation between Estrous Intensity and Estradiol Concentration on Local Cattle during Insemination)

2016 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Mauridatun Ramli ◽  
Tongku Nizwan Siregar ◽  
Cut Nila Thasmi ◽  
Dasrul Dasrul ◽  
Sri Wahyuni ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Jugular Vein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasma Samples ◽  
Hormone Concentration ◽  
Prostaglandin F2 Alpha ◽  
Commercial Kits ◽  
The Relationship ◽  
Estradiol Concentration

This study aims to determine the relationship between the intensity of estrus and estradiol concentrations during insemination on local cattle. Ten cows were divided into two groups with each group consist of five cows with category: aged 5-8 years, weight 150-250 kg, clinically healthy, and not pregnant. Plasma samples for examination of the hormone estradiol concentrations were collected during insemination time from jugular vein. Prior to the collection of plasma samples, whole cow were estrus synchronized using prostaglandin F2 alpha (PGF2α) and Ovsynch protocol. The PGF2α were injected twice at intervals of 11 days. Examination of estradiol hormone concentration was performed with enzyme-linked immunosorbent assay (ELISA) using estradiol commercial kits. The results of this study indicate that statistically there is no relationship between estrous intensity and estradiol concentration.

scholarly journals Perbandingan Kinerja Berahi dan Level Estradiol Kambing Kacang dan Kambing Nubian yang Diinduksi dengan PGF2α

2018 ◽  
Vol 36 (1) ◽  
pp. 32
Author(s):  
Mulyadi Adam ◽  
Raja Nurul Huda ◽  
Widya Zahara ◽  
Tongku Niswan Siregar ◽  
Sri Wahyuni ◽  
...  
Keyword(s):  
Estradiol Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Scoring Method ◽  
Prostaglandin F2 Alpha ◽  
Group 2 ◽  
First Time ◽  
The Relationship ◽  
Estradiol Concentration

The experiment was conducted to compare estrous performance between and estradiol level kacang and Nubian goats estrous induction by prostaglandin F2 alpha (PGF2α). Eight female goats were divided into two groups, groups 1 (K1) consisted five Nubian goats and group 2 (K2) included three kacang goats with criterias as follows: age 1.5-3.0 years old, not pregnant, at least two months postpartum, and healthy clinically. All goats were estrous synchronized by double injection 7,5 mg PGF2α intramuscularly with 10 days interval. Goats that appeared estrus symptoms were mated naturally to a male goat. Determination of estrous intensity was performed using scoring method. Blood samples were taken at the first time the goats were mated (day 0). The measurement of estradiol concentration was done by enzyme linked immunosorbent assay (ELISA) method. Data of intensity, onset, duration, and estradiol level were analyzed using t test. The results showed that the rate of intensity, onset, duration, and estradiol level of estrous at KI vs KII were 2.08±0.52 vs 1.85±0.67, 56.00±34.11 vs 40.80±18.20 hours, and 24.00±26.15 vs 33.80±9.10 hours, and 103.51±42.49 vs 67.95±38.44 pg/ml (P>0,05). It can be concluded there was no difference of estrous performance and estradiol level between kacang and Nubian which estrous induction with PGF2α. The relationship between the intensity of estrus estradiol level in the Nubian goats was 0.995 while the kacang goats was 0.890.

scholarly journals Autoimmune antibodies correlate with immune checkpoint therapy-induced toxicities

2019 ◽  
Vol 116 (44) ◽  
pp. 22246-22251 ◽  
Author(s):  
Salahaldin A. Tahir ◽  
Jianjun Gao ◽  
Yuji Miura ◽  
Jorge Blando ◽  
Rebecca S. S. Tidwell ◽  
...  
Keyword(s):  
Adverse Events ◽  
Cancer Patients ◽  
Immunosorbent Assay ◽  
Immune Checkpoint ◽  
Additional Patient ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasma Samples ◽  
Serological Analysis ◽  
Autoimmune Antibodies ◽  
Expression Libraries

Immune checkpoint (IC) therapy provides substantial benefits to cancer patients but can also cause distinctive toxicities termed immune-related adverse events (irAEs). Biomarkers to predict toxicities will be necessary to improve management of patients receiving IC therapy. We relied on serological analysis of recombinant cDNA expression libraries to evaluate plasma samples from patients treated with IC therapy and identified autoantibodies, both in pretreatment and on-treatment samples prior to the development of irAEs, which correlate with the development of immune-related hypophysitis (anti-GNAL and anti-ITM2B autoantibodies) and pneumonitis (anti-CD74 autoantibody). We developed an enzyme-linked immunosorbent assay and tested additional patient samples to confirm our initial findings. Collectively, our data suggest that autoantibodies may correlate with irAEs related to IC therapy, and specific autoantibodies may be detected early for the management of irAEs.

scholarly journals Comparative study between ECL and ELISA to determine the reliable range of Estradiol in the treatment of infertility

Bionatura ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 1352-1355
Author(s):  
Ali Sadeghitabar ◽  
Narges Maleki ◽  
Maryam Armand ◽  
Nasiri Reza
Keyword(s):  
Human Subjects ◽  
Pearson Correlation ◽  
Diagnostic Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
P Value ◽  
Vitro Fertilization ◽  
The Relationship ◽  
Different Levels ◽  
Estradiol Concentration

Estradiol is one factor that can alter the outcome of the treatment of infertile couples following the application of in vitro fertilization techniques. Currently, the estradiol level is measured by two diagnostic methods Enzyme-Linked Immunosorbent Assay (ELISA) and Electrochemiluminescence (ECL). Accordingly, this study determines ELISA and ECL's sensitivity and consistency to measure different levels of estradiol and determine its reliable range and provide this information to laboratories and gynecologists. This study is performed on 250 patients of the Avicenna Fertility Center. The data of the study are analyzed in SPSS18 and MiniTab. Consent was obtained for experimentation with human subjects. Pearson correlation coefficient was used to investigate the relationship between these two methods. The results indicated a strong correlation between the two variables ECL and ELISA ( r= 0.735, P-value<0.001). High numbers indicate that the decrease and increase of one variable are proportional to the other variable's fluctuation. This study shows that the results of estradiol obtained from both ECL and ELISA are similar. In the ELISA method, due to the linear values' limitation, samples with estradiol concentration above the highest standard level should be diluted and the dilution coefficient should then be applied.

Determination of Human Thrombin-Antithrombin III Complex in Plasma with an Enzyme-Linked Immunosorbent Assay

1988 ◽  
Vol 59 (01) ◽  
pp. 101-106 ◽  
Author(s):  
Hermann Pelzer ◽  
Angela Schwarz ◽  
Norbert Heimburger
Keyword(s):  
Immunosorbent Assay ◽  
Antithrombin Iii ◽  
Test System ◽  
Mean Value ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plasma Samples ◽  
Vein Thrombosis ◽  
Human Thrombin ◽  
Thrombin Antithrombin Iii Complex

SummaryAn enzyme-linked immunosorbent assay (ELISA) was developed for the determination of thrombin-antithrombin III complex (TAT) in human plasma. The test system follows the sandwich principle and uses two different antibodies directed against human thrombin and human antithrombin III, respectively. The antibodies bind selectively to the corresponding antigen moieties of TAT. The assay was calibrated with definite concentrations of preformed purified TAT added to TAT-poor plasma. The lower limit of sensitivity of the assay was 0.5 μg/1. Mean coefficients of variation of 4.2% (intraassay) and 3.5% (interassay) were found for TAT concentrations between 2 and 60 μg/1. A reference range from 0.85 to 3.2 μg/1 was calculated from TAT concentration in plasma samples from 88 healthy donors (mean value ± SD: 1.45 ± 0.4 μg/I). In plasma samples from patients with pulmonary embolism (n = 17), TAT concentrations between 3 and 25 μg/1 were measured. In 15 patients with deep vein thrombosis, TAT was found up to 3 to 25 μg/1. From these data we conclude that measurement of TAT can be a sensitive parameter for specific detection of a latent activation of the clotting pathway.

scholarly journals Development and Application of an Enzyme-Linked Immunosorbent Assay to Detect Antibodies against Prevalent Salmonella Serovars in Swine in Southern Brazil

2007 ◽  
Vol 19 (5) ◽  
pp. 510-517 ◽  
Author(s):  
Jalusa Deon Kich ◽  
Patrícia Schwarz ◽  
Luis Eduardo Silva ◽  
Arlei Coldebella ◽  
Itamar Antônio Piffer ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Cost Effective ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Production Chain ◽  
Control Programs ◽  
Serovar Typhimurium ◽  
Commercial Kits ◽  
Swine Herds

The implementation of Salmonella control programs in the pork production chain demands rapid and cost-effective methods to assess the prevalence of infection in pig herds. The objective of the present study was to develop an in-house enzyme-linked immunosorbent assay (ELISA) based on S. Typhimurium lipopolysaccharides (LPS) to measure the prevalence of infection caused by Salmonella in swine herds. Coating antigen was produced by phenol extraction of S. Typhimurium culture. After standardization of ELISA test conditions, the assay was validated by testing serum samples on different animal categories: pigs orally inoculated with S. Typhimurium and sentinel animals in contact with them, naturally infected animals, colostrum-deprived piglets, and bacterin-immunized pigs. Seroconversion was observed in inoculated pigs (7 days postinfection [DPI]) and in the sentinels (21 DPI). Nonspecific reactions were not detected in the sera of colostrum-deprived animals. Serum samples from animals immunized with Salmonella Agona, Salmonella Derby, Salmonella Panama, and Salmonella Bredeney bacterins showed marked cross-reaction with the LPS from the serovar Typhimurium. Moreover, positive results obtained with the in-house ELISA were associated with Salmonella isolation in 75 infected pig herds. Comparisons with 2 commercial kits showed a linear correlation coefficient of 0.847 between the in-house ELISA and kit A and 0.922 with kit B but a low agreement in the qualitative results. In conclusion, the newly developed in-house ELISA based on S. Typhimurium LPS can be a useful tool to determine the intensity of Salmonella sp. infection in swine herds.

scholarly journals CORRELATION BETWEEN PROGESTERONE HORMONE CONCENTRATION AND NUMBER OF FETUSES OF ETAWA CROSSBRED GOAT IN DIFFERENT GESTATION PERIOD

2017 ◽  
Vol 11 (3) ◽  
Author(s):  
Syafruddin Syafruddin ◽  
Khairul Rizal ◽  
Rosmaidar Rosmaidar ◽  
Juli Melia ◽  
Roslizawaty Roslizawaty ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Prostaglandin F2α ◽  
Gestation Period ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hormone Concentration ◽  
Progesterone Concentration ◽  
Blood Samples ◽  
The Difference ◽  
Highly Correlated

This study aimed to investigate the correlation between progesterone hormone concentration and number of fetus as well as the difference in progesterone hormone concentration from different gestation periods in Etawah crossbred (EC). Five EC goats used in this study were injected with 2 mL of 5.5 mg prostaglandin F2α (PGF2α, Capriglandin) intramuscularly, with an interval of 10 days. Goats that showed symptoms of estrus were mated naturally with male goats. Blood samples were taken on the 7th, 14th, 21st, and 75th days of gestation. Progesterone hormone concentration was determined using enzyme linked immunosorbent assay (ELISA). The number of fetus was determined at 35th gestational day using ultrasonography (USG). Four out of 5 goats were found to have 1 fetus and 1 goat had 2 fetuses. The progesterone concentration on the 7th, 14th, 21st, and 75th days of gestation in goats with single fetus were 5.21, 7.78, 11.97, and 18.78 ng/mL, respectively, while in goat with two fetuses were 8.44, 14.53, 16.81, and 22.73 ng/mL, respectively. The correlation (r) between progesterone hormone concentration and number of fetus on the 7th, 14th, 21st, and 75th days of gestation were 0.442, 0.854, 0.592, and 0.757, respectively. It is concluded that progesterone concentrations are highly correlated to the number of fetus in each gestation period in EC goats.

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