scholarly journals Pengaruh Pemberian ZPT BAP dan GA3 terhadap Pertumbuhan Tunas Ubi Jalar (Ipomea batatas L.) Varietas Cilembu secara In Vitro

2021 ◽  
Vol 21 (2) ◽  
pp. 124-128
Author(s):  
Rudi Wardana ◽  
Roayatus Syafa’ah ◽  
Jumiatun Jumiatun
Keyword(s):  
Ipomea Batatas

Cilembu merupakan ubi jalar yang memiliki rasa manis dan legit yang tidak diolah oleh jenis ubi jalar lainnya. Cilembu memiliki kandungan karbohidrat, vitamin dan mineral yang tinggi. Oleh karena itu tanaman ini berpotensi sebagai pengganti beras dalam bentuk pangan alternatif. Permasalahan yang terjadi berupa berbagai kendala, teknik budidaya serta kualitas bibit tanaman cilembu yang buruk. Oleh karena itu, perlu dilakukan peningkatan kualitas sentra produksi dengan meningkatkan kebutuhan bibit berkualitas dalam teknik kultur jaringan. Penelitian ini dilakukan dengan cara menambahkan konsentrasi BAP dan GA3 untuk merangsang pertumbuhan eksplan cilembu. Penelitian ini dilaksanakan pada bulan September 2019 hingga Februari 2020 di Laboratorium Kultur Jaringan Politeknik Negeri Jember. Rancangan percobaan disusun menggunakan Rancangan Acak Lengkap dengan 2 faktor. Faktor pertama adalah konsentrasi BAP sebesar 1 ppm; 1,5 ppm; 2 ppm. Faktor kedua adalah konsentrasi GA3 0,1 ppm; 0,5 ppm; 1 ppm. Hasil penelitian menunjukkan penambahan GA3 dengan konsentrasi 0,5 pm berpengaruh terhadap kemunculan tunas yaitu 15 hari setelah tanam, sedangkan BAP sebesar 1 ppm berpengaruh terhadap jumlah akar yaitu 16 akar.

scholarly journals Calcium content, in vitro digestibility, and bioaccessibility in leaves of spinach (Spinacia oleracea), sweet potato (Ipomea batatas), and drumstick tree (Moringa oleifera)

F1000Research ◽  
2014 ◽  
Vol 3 ◽  
pp. 65 ◽  
Author(s):  
Jonathan C. Allen ◽  
Joseph Y. Issa ◽  
Weiting Cai
Keyword(s):  
Sweet Potato ◽  
Calcium Intake ◽  
Moringa Oleifera ◽  
Spinacia Oleracea ◽  
Calcium Content ◽  
In Vitro Digestibility ◽  
Ipomea Batatas ◽  
Drumstick Tree ◽  
Sweet Potato Leaves

Low calcium intake, poor calcium absorption, excessive calcium losses, or some combination of these factors contribute to calcium deficiency diseases. Calcium insufficiency is associated with osteoporosis, hypertension and colon cancer among other diseases. For individuals who do not have access to milk and dairy products, do not tolerate them, or prefer not to consume them, plants may be  alternative sources of calcium. However, calcium bioavailability may be low in plant foods because calcium forms complexes with oxalates, phytate and other competing minerals. The objective of this study was to compare the calcium content, digestibility and bioaccessibility in the leaves of spinach (Spinacia oleracea), sweet potato (Ipomea batatas), and drumstick tree (Moringa oleifera). Calcium content was analyzed in dry leaf powder by atomic absorption, followed by a two-stage in vitro digestion and dialysis against a mock serum solution to determine calcium digestibility and bioaccessibility. Moringa oleifera had higher calcium content than spinach and sweet potato leaves: 1.54±0.11% of dry matter for three Moringa samples (two African and one from India), and 0.99±0.001 and 1.06±0.001, respectively, in spinach and sweet potato leaves. The mean in vitro calcium digestibility was 1.62±0.08% in spinach, 3.4±0.68% in sweet potato leaves and 33.7±9.6% for Moringa. A dialysis system was designed to model bioaccessibility of calcium, revealing that bioaccessible calcium in sweet potato leaves was a non-significant 1.4 times higher and in Moringa was 9.2 to 19.4 times higher than in spinach. Therefore, the calcium contained in Moringa leaves does not appear to be associated with poorly bioavailable complexes such as oxalate. We confirmed previous reports that bioaccessibility of the calcium is low in spinach. These findings imply that increased utilization of Ipomea batatas and Moringa oleifera leaves might increase calcium intake in people in tropical and warm temperate regions where these plants grow, or these plants might become a valuable export crop.

scholarly journals Konsentrasi Sukrosa Dan Agar Di Dalam Media Pelestarian In-Vitro Ubi Jalar Var. Sukuh

Agrologia ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Jane K. J Laisina
Keyword(s):  
Sweet Potato ◽  
Leaf Number ◽  
Green Leaf ◽  
Root Number ◽  
Media Composition ◽  
Ipomea Batatas ◽  
In Vitro Preservation ◽  
The Media ◽  
Preservation Medium

The objective of this research was  to obtain suitable concentration of sucrose and agar in the sweet potato (Ipomea batatas (L) Lam) in vitro preservation medium, in order to make plant grow slowly and healthy. Experiment was done in Molecular Biologi Laboratory of PAU IPB. The experiment was arranged in factorial complete random design, using sweet potato var. Sukuh in order to know the effect of sucrose (40,50,60,70 and 80 g l-1) and agar (7 and 8 g l-1) which were added 1 g l-1 hyponex fertilizer 20-20-20. The experiment was replicated four times. Data were analyzed parametrically and non-parametrically. The result of these experiments showed the suitable conservation media was 1 g l-1 hyponex 20-20-20 + 60 g l-1sucrose + 7 g l-1 agar. Threfore, the media composition MS could produce high green inter nodes number, high root number and more than two green leaf number. This experiment also showed that MS media could be replaced by a cheaper and easily found conservation medium.

scholarly journals 4. Characteristics Of Some Potential Forages In Indonesia In Reducing Methane (Ch4) Emission From Ruminants: Benefits And Limitations

2016 ◽  
Vol 1 (1) ◽  
pp. 27-37
Author(s):  
Amriana Hifizah
Keyword(s):  
Condensed Tannin ◽  
Rumen Fermentation ◽  
Pennisetum Purpureum ◽  
Panicum Maximum ◽  
Ruminal Fermentation ◽  
Cassava Leaves ◽  
Ch4 Production ◽  
Ipomea Batatas ◽  
Setaria Sphacelata

Animal production can be more efficient and also sustainable if we reduce CH4 production from ruminal fermentation. One option is to find alternative forages that modify rumen fermentation. CH4 is not only harmful the environment but also means loss to the animals. All of the aspects of the issue is related to the condition of ruminant’s farm in Indonesia. Some other forages that are mainly fed as protein source to ruminants, are: cassava leaves, sweet potato leaves, soya bean leaves, tofu waste, leaves of Artocarpus heterophyllus, Musa paradisiaca L, Ipomea batatas. Roughage sources are hays of Panicum maximum, Pennisetum purpureum, and Setaria sphacelata and the concentrate sources mainly corn, rice bran and cassava waste, and corn cobs. However, there are very limited studies in finding alternative forages that can both increase animals productivity and also reduce CH4 production. Only forages relevant to Indonesia that have been studied in vitro is reviewed in this article, about its potential in reducing CH4 production from rumen fermentation. Even though some forages reduce CH4, it could negatively influence digestibility, hence less productivity. Some studies indicated that it was due to the fat content of the forage while others indicated that the concentration of the bioactive compounds such as condensed tannin influence the side effect of low CH4 ruminal production.

scholarly journals Contribuciones del Laboratorio de Cultivo de Tejidos Vegetales de Zamorano a la Micropropagación y Propagación de Plantas Libres de Patógenos

Ceiba ◽  
2013 ◽  
Vol 52 (1) ◽  
pp. 194-198
Author(s):  
María Alexandra Bravo ◽  
Dinie Espinal de Rueda
Keyword(s):  
Stevia Rebaudiana ◽  
Arundo Donax ◽  
Colocasia Esculenta ◽  
Saintpaulia Ionantha ◽  
Ipomea Batatas ◽  
Anthurium Andreanum

Zamorano inauguró el Laboratorio de Cultivo de Tejidos Vegetales en 1985, durante 27 años se ha investigado sobre propagación in vitro de cultivos ornamentales, agronómicos y maderables. Entre los ornamentales más investigados está la orquídea Rhyncholaelia digbyana, flor nacional de Honduras, y varias especies nativas de la región. También se ha trabajado en violeta africana (Saintpaulia ionantha), zamias (Zamioculcas zamiifolia), helechos (Nephrolepis cordifolia) y anturios (Anthurium andreanum), cultivos apreciados en el mercado local de ornamentales y que son producto de las prácticas del aprender haciendo de nuestros estudiantes. Los últimos cuatro años se ha investigado y producido plántulas de especies económicamente importantes para la industria y la producción de energía como la caña de azúcar y el Arundo donax; para procesos de extracción de compuestos como la Stevia rebaudiana; para la exportación como malanga (Colocasia esculenta), y camote (Ipomea batatas). Se ha contribuido con los pequeños y medianos productores y exportadores de camote con aproximadamente 3000 plantas por año de camote libres de virus. DOI: http://dx.doi.org/10.5377/ceiba.v52i1.1130

scholarly journals Pelestarian Secara In Vitro Melalui Metode Pertumbuhan Lambat Pada Beberapa Genotipe Ubi Jalar (Ipomea Batatas (L) Lam)

Agrologia ◽  
2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Jean K J Laisina
Keyword(s):  
Sweet Potato ◽  
Slow Growth ◽  
Single Factor ◽  
Randomized Design ◽  
Ipomea Batatas ◽  
In Vitro Preservation ◽  
Potato Genotype ◽  
Completely Randomized Design ◽  
Growth Preservation

The objectives of this research were to estimate responses of several sweet potato genotypes in preservation media through in vitro slow growth preservation, and to achieve cheap and accessible media. Experiment was arranged in a completely randomized design with a single factor. The experimental factor was sweet potato genotype (Sukuh, 421.34,343.15 and 2040.8). These genotype were tested in a preservation media which consisted of 15% coconut water +  30 mg/l aspirin + 50 g/l sucrose + 7 g/l agar. Experiment was replicated four times. Data was analyzed parametrically and non-parametrically. The result showed that genotype gave high responses to preservation media. Genotype Sukuh produced highest leaves while genotype 421.34 yielded highest numbers of root and internode. In the preservation medium of Genotype Sukuh through in vitro slow growth preservation, aspirin could be added to inhibit growth by increased leaf senesence. This experiment also showed that Hiponex (20:20:20) could be used as the basic media for in vitro preservation of sweet potato

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

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