In vitro Regeneration of Sweet Potato (Ipomea batatas (L.) Lam.) from Node Explants

2015 ◽  
Vol 8 (2) ◽  
pp. 87-92
Author(s):  
N. Onwubiko ◽  
C. Ihezie ◽  
M. Mozie
Keyword(s):  
Sweet Potato ◽  
In Vitro Regeneration ◽  
Ipomea Batatas

scholarly journals Calcium content, in vitro digestibility, and bioaccessibility in leaves of spinach (Spinacia oleracea), sweet potato (Ipomea batatas), and drumstick tree (Moringa oleifera)

F1000Research ◽  
2014 ◽  
Vol 3 ◽  
pp. 65 ◽  
Author(s):  
Jonathan C. Allen ◽  
Joseph Y. Issa ◽  
Weiting Cai
Keyword(s):  
Sweet Potato ◽  
Calcium Intake ◽  
Moringa Oleifera ◽  
Spinacia Oleracea ◽  
Calcium Content ◽  
In Vitro Digestibility ◽  
Ipomea Batatas ◽  
Drumstick Tree ◽  
Sweet Potato Leaves

Low calcium intake, poor calcium absorption, excessive calcium losses, or some combination of these factors contribute to calcium deficiency diseases. Calcium insufficiency is associated with osteoporosis, hypertension and colon cancer among other diseases. For individuals who do not have access to milk and dairy products, do not tolerate them, or prefer not to consume them, plants may be  alternative sources of calcium. However, calcium bioavailability may be low in plant foods because calcium forms complexes with oxalates, phytate and other competing minerals. The objective of this study was to compare the calcium content, digestibility and bioaccessibility in the leaves of spinach (Spinacia oleracea), sweet potato (Ipomea batatas), and drumstick tree (Moringa oleifera). Calcium content was analyzed in dry leaf powder by atomic absorption, followed by a two-stage in vitro digestion and dialysis against a mock serum solution to determine calcium digestibility and bioaccessibility. Moringa oleifera had higher calcium content than spinach and sweet potato leaves: 1.54±0.11% of dry matter for three Moringa samples (two African and one from India), and 0.99±0.001 and 1.06±0.001, respectively, in spinach and sweet potato leaves. The mean in vitro calcium digestibility was 1.62±0.08% in spinach, 3.4±0.68% in sweet potato leaves and 33.7±9.6% for Moringa. A dialysis system was designed to model bioaccessibility of calcium, revealing that bioaccessible calcium in sweet potato leaves was a non-significant 1.4 times higher and in Moringa was 9.2 to 19.4 times higher than in spinach. Therefore, the calcium contained in Moringa leaves does not appear to be associated with poorly bioavailable complexes such as oxalate. We confirmed previous reports that bioaccessibility of the calcium is low in spinach. These findings imply that increased utilization of Ipomea batatas and Moringa oleifera leaves might increase calcium intake in people in tropical and warm temperate regions where these plants grow, or these plants might become a valuable export crop.

scholarly journals Influence of Mercuric Chloride on Survival and Suitability for In Vitro Regeneration of Three Sweet Potato Landraces (Ipomoea batatas L.) Produced in Benin

2017 ◽  
Vol 4 (1) ◽  
pp. 56-64
Author(s):  
Arsène M. Doussoh ◽  
Justine Dangou-Sossou ◽  
Serge S. Houédjissin ◽  
Gilles H. T. Cacaï ◽  
Armel K. Assogba ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Mercuric Chloride ◽  
Ipomoea Batatas ◽  
In Vitro Regeneration

scholarly journals Konsentrasi Sukrosa Dan Agar Di Dalam Media Pelestarian In-Vitro Ubi Jalar Var. Sukuh

Agrologia ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Jane K. J Laisina
Keyword(s):  
Sweet Potato ◽  
Leaf Number ◽  
Green Leaf ◽  
Root Number ◽  
Media Composition ◽  
Ipomea Batatas ◽  
In Vitro Preservation ◽  
The Media ◽  
Preservation Medium

The objective of this research was  to obtain suitable concentration of sucrose and agar in the sweet potato (Ipomea batatas (L) Lam) in vitro preservation medium, in order to make plant grow slowly and healthy. Experiment was done in Molecular Biologi Laboratory of PAU IPB. The experiment was arranged in factorial complete random design, using sweet potato var. Sukuh in order to know the effect of sucrose (40,50,60,70 and 80 g l-1) and agar (7 and 8 g l-1) which were added 1 g l-1 hyponex fertilizer 20-20-20. The experiment was replicated four times. Data were analyzed parametrically and non-parametrically. The result of these experiments showed the suitable conservation media was 1 g l-1 hyponex 20-20-20 + 60 g l-1sucrose + 7 g l-1 agar. Threfore, the media composition MS could produce high green inter nodes number, high root number and more than two green leaf number. This experiment also showed that MS media could be replaced by a cheaper and easily found conservation medium.

scholarly journals Standardization of plant growth regulators for successful tissue culture of sweet potato

2018 ◽  
Vol 16 (2) ◽  
pp. 178-181
Author(s):  
Jahanara Parvin ◽  
Mahbub Robbani ◽  
Md Fakhrul Hasan ◽  
Farhana Hoque
Keyword(s):  
Sweet Potato ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Naphthalene Acetic Acid ◽  
Nodal Segment ◽  
Ms Medium ◽  
Root Initiation ◽  
Shoot Initiation ◽  
Minimum Number

The experiment was carried out during the period from June 2016 to January, 2017 at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University (PSTU) for in vitro regeneration of sweet potato. In this study, the nodal segment explants and different combinations of growth regulators were used for in vitro regeneration of sweet potato. BAP (6-benzylamino purine) and KIN (Kinetin) were used for in vitro shoot regeneration and IBA (Indole-6- Butyric Acid) with NAA (Naphthalene Acetic Acid) were used for in vitro root regeneration. The highest percentage of shoot initiation (91.30), the minimum number of days (9.00) for shoot initiation, the highest number of shoot/plantlet (11.00) and the highest shoot length (4.38 cm) was observed when nodal segment explants cultured on MS medium supplemented with BAP 1.5 mg/L + KIN 0.1 mg/L. On the other hand, the highest root initiation percentage (94.12), the minimum number of days (6.00) for root initiation, the highest number of root/plantlet (9.33) and the highest root length (11.13 cm) was observed in the MS medium supplemented with IBA 0.5 mg/L + NAA 0.1 mg/L. Regenerated plantlets were acclimatized for 5 days and adopted in soil by 6-7 days of transplantation.J. Bangladesh Agril. Univ. 16(2): 178-181, August 2018

scholarly journals Pelestarian Secara In Vitro Melalui Metode Pertumbuhan Lambat Pada Beberapa Genotipe Ubi Jalar (Ipomea Batatas (L) Lam)

Agrologia ◽  
2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Jean K J Laisina
Keyword(s):  
Sweet Potato ◽  
Slow Growth ◽  
Single Factor ◽  
Randomized Design ◽  
Ipomea Batatas ◽  
In Vitro Preservation ◽  
Potato Genotype ◽  
Completely Randomized Design ◽  
Growth Preservation

The objectives of this research were to estimate responses of several sweet potato genotypes in preservation media through in vitro slow growth preservation, and to achieve cheap and accessible media. Experiment was arranged in a completely randomized design with a single factor. The experimental factor was sweet potato genotype (Sukuh, 421.34,343.15 and 2040.8). These genotype were tested in a preservation media which consisted of 15% coconut water +  30 mg/l aspirin + 50 g/l sucrose + 7 g/l agar. Experiment was replicated four times. Data was analyzed parametrically and non-parametrically. The result showed that genotype gave high responses to preservation media. Genotype Sukuh produced highest leaves while genotype 421.34 yielded highest numbers of root and internode. In the preservation medium of Genotype Sukuh through in vitro slow growth preservation, aspirin could be added to inhibit growth by increased leaf senesence. This experiment also showed that Hiponex (20:20:20) could be used as the basic media for in vitro preservation of sweet potato

scholarly journals In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam.) cultivars

2018 ◽  
Author(s):  
A. S. Abubakar ◽  
S. U. Yahaya ◽  
A. S. Shaibu ◽  
S. U. Yahaya ◽  
H. Ibrahim ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
In Vitro Regeneration ◽  
Naphthalene Acetic Acid ◽  
Shoot Height ◽  
Mean Values ◽  
Significant Difference ◽  
The Media ◽  
Planting Materials

The study is aimed at establishing a simple protocol for in vitro regeneration of sweet potato with a view to providing planting materials to farmers as well as basis for genetic improvement. Axillary buds were excised and cultured on Murashige and Skoog (MS) basal salts supplemented with 6-benzyl aminopurine (BAP), gibberellic acid (GA3) and naphthalene acetic acid (NAA) singly or in combination. The shoot height and number of leaves differed significantly among the cultivars. The result also indicated significant difference (p less than 0.01) among the cultivars with King J recording the highest mean values. Significant differences (p less than 0.05) was also recorded in the media combination with respect to organogenesis and number of shoots obtained. The results of hardening further revealed 33.33% success in the explants transferred directly to the field, as well as for the plantlets that were gradually weaned in a mixture of 3:1 sand and biochar.

Composition of White Potato Starch (Ipomea batatas L.) with Avocado Seed Starch (Persea americana Mill) and Glyserol Concentration in Edible Film

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Musdar Musdar ◽  
Lukmanul Hakim ◽  
Juliani Juliani ◽  
Jailani Jailani
Keyword(s):  
Sweet Potato ◽  
Potato Starch ◽  
Persea Americana ◽  
Edible Film ◽  
Glycerol Concentration ◽  
White Potato ◽  
Ipomea Batatas ◽  
Sweet Potato Starch ◽  
Local Plants ◽  
Avocado Seed

White sweet potato starch (Ipomea batatas L.) and avocado seed starch (Parsea americana Mill) derived from local plants have the potential to be developed as agricultural products. Starch is a hydrocolloid compound as a potential local resource to be utilized. Glycerol function as an anti-freezing which is hygroscopic. This study aims to determine the ratio of white sweet potato starch with avocado seed starch and the concentration of glycerol for making edible film. This study was an experiment using a completely randimized factorial design with 2 (two) main factor consisting of a comparison of white sweet potato starch and avocado seed with 3 levels: P1 = 35%:65%., P2=50%:50%., P3=65%:35% and glycerol concentration with 3 levels: G1=1%., G2=2%., G3=3%. The best result reasearch were content of 23.03% (tratment P1G1), solubility of 55.57% (treatment P3G2)., swelling test of 9.83% (treatment P2g3)., elongation of 8.18% (treatment P3G2)

Sign in / Sign up

Export Citation Format

Share Document