scholarly journals Effect of bacterial lipopolysaccharide in primary cultures of human gingival fibroblasts

2017 ◽  
Vol 14 (2) ◽  
Author(s):  
Maria Cecilia Verutti ◽  
Octavio González ◽  
John González ◽  
Gloria Moreno
Keyword(s):  
Healthy Donor ◽  
In Vitro Model ◽  
Periodontal Diseases ◽  
Primary Cultures ◽  
Cell Number ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Short Period ◽  
Vitro Model

Summary: Introduction: different factors participate in the pathogenesis ofperiodontal diseases. One factor is the interaction between the fibroblasts and derived productsfrom the microorganisms found in the periodontal environment. Objective: In this work, cultures ofhuman gingival fibroblasts from a healthy donor were used to characterize the in vitro responses tobacterial lipopolysaccharide. Methods: the proliferative response was evaluated using cell count andexpression of CD14 was assessed by flow cytometry. Results: after 24 hours of culture an increase inthe cell number was detected in cultures treated with 1.0 mg/mL LPS, but these differences were notstatistically significant. Human gingival fibroblasts express CD14, but its expression decreases incells cultivated after a short period of time. Nevertheless, lipopolysaccharide helps to recover theexpression of CD14 in fibroblast after 24 hours of culture. Conclusion: preliminary result suggeststhat expression of CD14 on gingival fibroblasts could be modulated by this bacterial derived toxin.The primary culture of human gingival fibroblasts allows the establishment of an in vitro model toevaluate different processes in development of the periodontal diseases. Key words: Gingivalfibroblasts. Lipopolysaccharide. Periodontal disease.

Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts

2017 ◽  
Vol 12 (5) ◽  
pp. 055005 ◽  
Author(s):  
Patrizia De Marco ◽  
Susi Zara ◽  
Marianna De Colli ◽  
Milena Radunovic ◽  
Vladimir Lazović ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
In Vitro Model ◽  
Gingival Fibroblasts ◽  
Collagen Membranes ◽  
Vitro Model

scholarly journals An in vitro feasibility investigation considering primary human melanocytes for spray- grafting of freshly isolated autologous skin cells for burn treatment and a clinical case report

2019 ◽  
pp. 1-8
Author(s):  
Jörg C. Gerlach ◽  
C. Johnen ◽  
B. Hartmann ◽  
J. Plettig ◽  
K. Bräutigam ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Skin Color ◽  
In Vitro Model ◽  
Primary Cultures ◽  
Cell Suspensions ◽  
Epidermal Keratinocytes ◽  
Cell Counts ◽  
Skin Coloration ◽  
Vitro Model

A skin cell-spray grafting technique that enables the on-site application of freshly isolated autologous single cell suspensions was already applied in many cases on caucasian patients with low skin coloration. Our project hypothesis is that these suspensions contain keratinocytes and vital melanocytes, that are of particular interest for the treatment of patients of darker skin color. To test this, we applied an in vitro model, wherein the feasibility of i) isolating and ii) spraying of freshly isolated autologous melanocyte-keratinocyte cell suspensions was investigated. Primary human epidermal keratinocytes (HEKs) and melanocytes (MCs) were isolated from skin biopsies (n=8). Biochemical parameter, cell counts, cell morphology, growth behavior and immunofluorescence results were compared in two groups using MC cultures and co-cultures of MCs with HEKs. Case information on using the method clinically with one patient is included. The sprayed mixed cell suspensions proliferated in all groups without measurable loss of viability, and MCs exhibited a regular cell morphology in monoculture up to passage 4°. The sprayed MCs and HEKs demonstrated in vitro glucose and lactate metabolism that was comparable to the pipetted controls. In co-culture, well distributed CK14+ HEKs and NKI/beteb+ MCs could be demonstrated, which interacted in the in vitro model. The ratio of HEKs : MCs in our primary cultures were microscopically counted (n=8 each) as mean +/- SD 1,211,000 (+/- 574,343) HEK : 99,625 (+/- 59,025) MC; i.e., a ratio of approx. 12 : 1. Using the isolation method clinically for a patient with dark skin coloration after suffering severe second-degree burns shows a satisfying re-pigmentation of the resulting wound post healing. Freshly isolated spray-on melanocyte/keratinocyte suspensions provide for a considerable amount of viable HEKs and MCs. Using MCs in spray-grafting suspensions could represent a promising approach for treating severe partial-thickness burns and innovative therapy developments that also aim to address cosmetic aspects.

scholarly journals Atlantic salmon cardiac primary cultures: An in vitro model to study viral host pathogen interactions and pathogenesis

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181058 ◽  
Author(s):  
Patricia A. Noguera ◽  
Bianka Grunow ◽  
Matthias Klinger ◽  
Katherine Lester ◽  
Bertrand Collet ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
In Vitro Model ◽  
Primary Cultures ◽  
Host Pathogen Interactions ◽  
Host Pathogen ◽  
Vitro Model

scholarly journals In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials

2016 ◽  
Vol 17 (6) ◽  
pp. 457-462 ◽  
Author(s):  
Isleine P Caldas ◽  
Miriam Z Scelza ◽  
Marco A Gallito ◽  
Gutemberg Alves ◽  
Licínio Silva
Keyword(s):  
Cell Viability ◽  
Human Fibroblasts ◽  
Primary Cultures ◽  
Polystyrene Latex ◽  
Test Method ◽  
Human Gingival Fibroblasts ◽  
Control Group ◽  
Gingival Fibroblasts ◽  
Significant Difference

ABSTRACT Aims The aim of this study is to evaluate the in vitro response of human gingival fibroblasts in primary cultures to two materials for temporary relining of dentures: Temporary Soft (TDV, Brazil) and Trusoft (Bosworth, USA) for 24 hours, 7 and 30 days by using a multi-parametric analysis. Materials and methods Each material sample (TDV, TS, Polystyrene, Latex) was prepared and incubated in a culture medium for 1, 7, and 30 days at 37°C. Human gingival fibroblasts were exposed to the extracts and cell viability was evaluated by a multi-parametric assay, which allowed sequential analysis of mitochondrial activity (XTT), membrane integrity [neutral red (NR)], and cell density [crystal violet dye exclusion (CVDE)] in the same cells. Analysis of variance (ANOVA) was used to test the interactions of the three sources of variation (material, test method, and time) with the proportions of viable cells for each relining material. Results Both evaluated materials (TDV and TS) had low cytotoxic effects during 1, 7, and 30 days after manipulation of the material, as assessed by all three methods used. A statistical difference was found when comparing the negative control group (latex fragments) with the other groups, which showed high toxicity and low percentage of cell viability in all tests used. There was no significant difference among other materials (p > 0.05). Conclusion Low cytotoxicity levels were detected by representatives of the major groups of temporary prosthetic relining materials, as evaluated by multiple cellular viability parameters in human fibroblasts. Clinical significance There are various soft materials on the market for relining prostheses; however, the effects of these materials on tissues need to be clarified to avoid problems for patients. How to cite this article Caldas IP, Scelza MZ, Gallito MA, Alves G, Silva L. In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials. J Contemp Dent Pract 2016;17(6):457-462.

Renal cell carcinoma primary cultures as in vitro model to study genomic profile of parental tumor tissues

2008 ◽  
Vol 6 (9) ◽  
pp. 117
Author(s):  
I. Cifola ◽  
C. Bianchi ◽  
E. Mangano ◽  
S. Bombelli ◽  
S. Ferrero ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
In Vitro Model ◽  
Primary Cultures ◽  
Genomic Profile ◽  
Tumor Tissues ◽  
Parental Tumor ◽  
Vitro Model

scholarly journals Ovine fetuses from slaughterhouses: A useful source for neural cell primary cultures

2021 ◽  
Author(s):  
Vittorio Farina ◽  
Sergio Domenico Gadau ◽  
Gianluca Lepore ◽  
Marcella Carcupino ◽  
Marco Zedda
Keyword(s):  
In Vitro Model ◽  
Primary Cultures ◽  
Medical Procedures ◽  
Cell Type ◽  
Experimental Animals ◽  
Vitro Model ◽  
Set Up ◽  
Very High ◽  
Β Tubulin

A lot of evidence demonstrates that sheep could represent an experimental model to set up medical procedures in view of their application on humans. Sheep are chosen as models for human biomechanical studies because their skeleton has some similarities to humans. The aim of this work was to set up sheep primary cultures from ovine fetuses at different ages, from pregnant uteri retrieved at local abattoirs. Cell characterization showed that one cell population was immunopositive to GFAP and identifiable as astrocytes, whereas a second cell type was III β-tubulin-positive, and hence classified as neurons. At 60-day old fetus is suitable to obtain neurons, whereas in a 90-day old fetus the cell culture is predominantly characterized by glial cells. The procedure here proposed is inexpensive, in fact, collecting fetuses during sheep slaughtering is a cost-saving option, unlike common experimental animals such as mice, rats, rabbits, that require very high economical efforts. Finally, our protocol fully eliminates the need of animal killing, being living animals replaced by a validated in vitro model in agreement with the 3Rs statement.

An In Vitro Model of Differentiated Human Airway Epithelia: Methods for Establishing Primary Cultures

2003 ◽  
pp. 115-137 ◽  
Author(s):  
Philip H. Karp ◽  
Thomas O. Moninger ◽  
S. Pary Weber ◽  
Tamara S. Nesselhauf ◽  
Janice L. Launspach ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Primary Cultures ◽  
Airway Epithelia ◽  
Human Airway ◽  
Vitro Model ◽  
Human Airway Epithelia
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