Protective effect of citrus honey toward the histopathological and biochemical nephrotoxicity damages of gentamicin–induced in local rabbits Oryctolagus cuniculus

Drugs induced nephrotoxicity now considered as an essential reason for kidney failure. Aminoglycoside anti-infection agents, for example, gentamicin, which causes ototoxicity and nephrtotoxicity as a side effect , this research is centered around the utilization of natural materials as an antioxidants against the lethal oxidative activity that applied on the kidney cells ,the most imperative one of these materials is the honey. This work aimed to assess the defensive impact of citrus honey against the histopathological and biochemical harms of gentamicin induced nephrotoxicity .24 locally breed rabbits (Oryctolagus cuniculus) were used and divided into 4 study groups (6 rabbits per each group), group 1 received I.P daily dose of normal saline (control), group 2 received (80 mg/kg/day) I.P dose of gentamicin, group 3 received (80mg/kg/day) of gentamicin I.P dose in combination with oral dose of Citrus Honey625 mg/kg/day for 14 days and group 4 received (80mg/kg/day) of gentamicin I.P dose in combination with extra dose of Citrus Honey orally for 14 days. All animals (at 15th day) were anesthetized by ether and sacrificed; blood samples were gathered for the subsequent measurement of the serum creatinine, urea and albumin while an isolated kidney was kept in 10 % of formaldehyde for the histopathological examination. The results demonstrated that gentamicin causes nephrotoxicity showen by elevation of serum level of creatinine, urea and a decrease in the serum albumin. While the administration of honey in combination with gentamicin reduced the nephro-toxic effect of gentamicin that represented by a reduction of the serum creatinine and urea with improvement of the kidney histological findings.This study concluded that, honey decreased nephrotoxic effect of gentamicin http://dx.doi.org/10.25130/tjps.25.2020.001

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The Effect of Iloprost and N-Acetylcysteine on Skeletal Muscle Injury in an Acute Aortic Ischemia-Reperfusion Model: An Experimental Study

BioMed Research International ◽

10.1155/2015/453748 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Osman Tiryakioglu ◽

Kamuran Erkoc ◽

Bulent Tunerir ◽

Onur Uysal ◽

H. Firat Altin ◽

...

Keyword(s):

Tissue Injury ◽

Histopathological Examination ◽

Ischemia Reperfusion ◽

Control Group ◽

Group 4 ◽

Reperfusion Period ◽

Group 3 ◽

Group 2 ◽

Iloprost Infusion ◽

Group 1

Objective. The objective of this study was to examine the effects of iloprost and N-acetylcysteine (NAC) on ischemia-reperfusion (IR) injuries to the gastrocnemius muscle, following the occlusion-reperfusion period in the abdominal aorta of rats.Materials and Methods. Forty male Sprague-Dawley rats were randomly divided into four equal groups.Group 1: control group. Group 2 (IR): aorta was occluded. The clamp was removed after 1 hour of ischemia. Blood samples and muscle tissue specimens were collected following a 2-hour reperfusion period.Group 3 (IR + iloprost): during a 1-hour ischemia period, iloprost infusion was initiated from the jugular catheter. During a 2-hour reperfusion period, the iloprost infusion continued.Group 4 (IR + NAC): similar to the iloprost group.Findings. The mean total oxidant status, CK, and LDH levels were highest in Group 2 and lowest in Group 1. The levels of these parameters in Group 3 and Group 4 were lower compared to Group 2 and higher compared to Group 1 (P<0.05). The histopathological examination showed that Group 3 and Group 4, compared to Group 2, had preserved appearance with respect to hemorrhage, necrosis, loss of nuclei, infiltration, and similar parameters.Conclusion. Iloprost and NAC are effective against ischemia-reperfusion injury and decrease ischemia-related tissue injury.

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Effects of Selenium and Melatonin on Ocular Ischemic Syndrome

BioMed Research International ◽

10.1155/2019/8080564 ◽

2019 ◽

Vol 2019 ◽

pp. 1-6

Author(s):

Hande Hüsniye Telek

Keyword(s):

Lipid Peroxidation ◽

Anterior Segment ◽

Study Groups ◽

Control Group ◽

Ocular Ischemic Syndrome ◽

Group 4 ◽

Ischemia Group ◽

Group 3 ◽

Group 2 ◽

Ischemic Syndrome

Purpose. To determine the effects of selenium, melatonin, and selenium + melatonin administered for one month on anterior chamber (AC) malondialdehyde (MDA) and AC glutathione (GSH) levels in patients with ocular ischemic syndrome. Materials and Methods. Thirty-five patients were included in the study. Study groups were formed as follows: (1) control group, (2) ischemia group, (3) selenium + ischemia group, (4) melatonin + ischemia group, and (5) selenium + melatonin + ischemia group. AC samples were obtained. MDA and GSH levels in AC samples were evaluated. Results. MDA levels were significantly increased in ischemia groups. Selenium and melatonin supplementation resulted in reduction of MDA levels and significant increase in GSH values. Discussion. Increased lipid peroxidation associated with ischemia of the anterior segment has been prevented by selenium and melatonin supplementation. This trial is registered with ClinicalTrials.gov NCT04005222.

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Antioxidants attenuate endotoxin-induced microvascular leakage of macromolecules in vivo

Journal of Applied Physiology ◽

10.1152/jappl.1991.70.4.1483 ◽

1991 ◽

Vol 70 (4) ◽

pp. 1483-1489 ◽

Cited By ~ 8

Author(s):

T. Matsuda ◽

C. A. Eccleston ◽

I. Rubinstein ◽

S. I. Rennard ◽

W. L. Joyner

Keyword(s):

Dimethyl Sulfoxide ◽

Cheek Pouch ◽

Saline Control ◽

Control Group ◽

Hamster Cheek Pouch ◽

Group 4 ◽

Group 3 ◽

Group 5 ◽

Group 2 ◽

Group 1

The purpose of this study was to examine whether antioxidants attenuate endotoxin-induced microvascular hyper-permeability for macromolecules in the hamster cheek pouch. Twenty-two adult male Syrian hamsters were anesthetized, and a removable plastic chamber was placed in the cheek pouch to observe and collect suffusate from the microvasculature. Fluorescent-labeled dextran (FITC-D; mol wt 150,000) was injected intravenously, and changes in the number of microvascular leaky sites and microvascular clearance of FITC-D were measured in five groups: saline control (group 1, n = 4), endotoxin (0.1 mg/ml) suffusion for 120 min (group 2, n = 6), endotoxin plus dimethyl sulfoxide (1.0 g/kg iv; group 3, n = 4), endotoxin plus allopurinol (30 mg/kg ip; group 4, n = 4), and endotoxin plus dimethyl sulfoxide and allopurinol (group 5, n = 4). The number of leaky sites and the FITC-D clearance were significantly higher in group 2 [45 +/- 18 (SD) sites/cm2 and 20 +/- 6 X 10(-6) ml/min, respectively; P less than 0.01] than in group 1 (7 +/- 6 sites/cm2 and 7 +/- 5 X 10(-6) ml/min), group 3 (9 +/- 5 sites/cm2 and 8 +/- 2 X 10(-6) ml/min), group 4 (11 +/- 7 sites/cm2 and 9 +/- 4 X 10(-6) ml/min), and group 5 (11 +/- 6 sites/cm2 and 7 +/- 1 x 10(-6) ml/min). The leaky sites appeared predominantly in postcapillary venules. There was a positive and significant correlation between the number of leaky sites and FITC-D clearance.(ABSTRACT TRUNCATED AT 250 WORDS)

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The Effect of Intramuscular Oxytocin Injection on Milk Ejection in Dairy Cattles

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v8i11.2405-2407.3686 ◽

2020 ◽

Vol 8 (11) ◽

pp. 2405-2407

Author(s):

Mansur Seymen Seğmenoğlu

Keyword(s):

Milk Yield ◽

Study Groups ◽

Milk Quality ◽

Control Group ◽

Not Given ◽

Milk Ejection ◽

Group 4 ◽

Group 3 ◽

Group 2 ◽

Group 1

Milking is a source of change in milk yield and milk quality determination, and one of the main events of milking is the oxytocin-mediated ejection reflex. Complete ejection of milk from the udder is due to the presence of high oxytocin during milking. The aim of this study is to investigate whether oxytocin administered intramuscularly has an effect on increasing milk ejection. In the study, 28 clinically healthy lactation Holstein cows were used, the lactation numbers and lactation periods of these cows were ignored in the evaluation. The study groups are divided into four groups, each consisting of seven cows. 50 IU oxytocin was given to group-1 five days before milking in the morning and evening, group-2 received 50 IU oxytocin once a day just before milking and the group-3 was given 50 IU oxytocin once before morning milking on the 1st, 3rd, and 5th days and the group-4 is a control group, oxytocin was not given to this group. Oxytocin injection was administered intramuscularly 30 minutes before milking. In the evaluation, the last five days of milk data before the application, five days of milk data during the application period and five days of milk data after the application period were taken into consideration. As a result of the study, we can say that the udder was not completely emptied with the intramuscular administration of oxytocin.

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Evaluation of Gingival Microleakage in Class II Composite Restorations with Different Lining Techniques: An In Vitro Study

Scientifica ◽

10.1155/2015/896507 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6

Author(s):

Vedavathi Bore Gowda ◽

B. V. Sreenivasa Murthy ◽

Swaroop Hegde ◽

Swapna Devarasanahalli Venkataramanaswamy ◽

Veena Suresh Pai ◽

...

Keyword(s):

Study Groups ◽

Class Ii ◽

Composite Liner ◽

Flowable Composite ◽

Composite Restorations ◽

Group 4 ◽

Group 3 ◽

Group 2 ◽

Group 1

Aim. To compare the microleakage in class II composite restorations without a liner/with resin modified glass ionomer and flowable composite liner.Method. Forty standardized MO cavities were prepared on human permanent mandibular molars extracted for periodontal reasons and then divided into 4 groups of ten specimens. The cavity preparations were etched, rinsed, blot dried, and light cured and Adper Single Bond 2 is applied. Group 1 is restored with Filtek P60 packable composite in 2 mm oblique increments. Group 2 is precure group where 1 mm Filtek Z350 flowable liner is applied and light cured for 20 sec. Group 3 is the same as Group 2, but the liner was cocured with packable composite. In Group 4, 1 mm RMGIC, Fuji Lining LC is applied and cured for 20 sec. All the teeth were restored as in Group 1. The specimens were coated with nail varnish leaving 1 mm around the restoration, subjected to thermocycling, basic fuchsin dye penetration, sectioned mesiodistally, and observed under a stereomicroscope.Results. The mean leakage scores of the individual study groups were Group 1 (33.40), Group 2 (7.85), Group 3 (16.40), and Group 4 (24.35). Group 1 without a liner showed maximum leakage. Flowable composite liner precured was the best.

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Antioxidant Effect of MnTE-2-PyP on Lung in Asthma Mice Model

The Scientific World JOURNAL ◽

10.1100/2012/379360 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 6

Author(s):

Lyudmil Terziev ◽

Violeta Dancheva ◽

Veneta Shopova ◽

Galya Stavreva

Keyword(s):

Superoxide Dismutase ◽

Glutathione Peroxidase ◽

Control Group ◽

Mice Model ◽

Antioxidant Defence ◽

Group 4 ◽

Antioxidant Defence System ◽

Group 3 ◽

Group 2 ◽

Group 1

Aim. To investigate the effects of MnTE-2-PyP on some markers of antioxidant defence system in asthma mice model.Material and Methods. The animals were divided into four groups: group 1, controls; group 2, injected with ovalbumin, group 3, treated with MnTE-2-PyP, and group 4, treated with ovalbumin and MnTE-2-PyP. The activities of superoxide dismutase, catalase, glutathione peroxidase and nonprotein sulfhydryl groups content (NPSH) were determined in lung homogenate.Results. The activities of superoxide dismutase and catalase in group 2 decreased significantly as compared to control group. The decrease of the same enzymes in group 4 was lower and significant as compared to group 2. Changes in the glutathione peroxidase activity showed a similar dynamics. The NPSH groups content decreased in group 2. In group 4 this decrease was relatively lower as compared to group 2.Conclusions. The application of MnTE-2-PyP mitigated the effects of oxidative stress in asthma mice model.

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Effectiveness of medical treatment in overcoming the ureteral double-J stent related symptoms

Canadian Urological Association Journal ◽

10.5489/cuaj.145 ◽

2012 ◽

Vol 6 (6) ◽

pp. 234 ◽

Cited By ~ 13

Author(s):

Ugur Kuyumcuoglu ◽

Bilal Eryildirim ◽

Murat Tuncer ◽

Gokhan Faydaci ◽

Fatih Tarhan ◽

...

Keyword(s):

The Body ◽

Control Group ◽

Group 4 ◽

Double J Stent ◽

Urinary Tract Symptoms ◽

Stone Treatment ◽

Group 3 ◽

Group 5 ◽

Group 2

Background: We investigated whether the frequency of lower urinary tract symptoms (LUTS) increased in patients in whom double-J stents were applied. We also evaluated several medical therapy protocols to treat symptoms related with ureteral stents.Materials and Methods: A total of 108 patients, in whom unilateral double-j stent was applied during ureteral stone treatment, were included. Before the double-J stent was applied, all patients completed storage components of the “International Prostate Symptom Score” (IPSSs), quality of life components of the IPSS (IPSS-QOL) and “Overactive Bladder Questionnaire” (OABq) forms and scores were calculated. After the procedure, cases were randomized into 5 groups, an antiinflammatory was given to Group 1, spasmolytic to Group 2, anticholinergic to Group 3 and α-blocker to Group 4. No additional drug was given to Group 5 as this control group. During the fourth week of the procedure, IPSSs, IPSS-QOL and OABq forms were again completed and scores were compared with the previous ones.Results: When all the cases were evaluated, the IPSSs, IPSS-QOLand OABq scores of patients in whom the double-J stent was applied were statistically significantly higher the procedure. Compared to the control group, the cases where the double-J stent was applied showed a higher IPSSs, IPSS-QOL and OABq scores and none of the medical therapies could prevent this increase.Interpretation: The frequency of LUTS increased in cases where the ureteral stent was applied and discomfort continued as long as the stent stayed in the body.

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Pengaruh Monoklonal Antibodi Bovine Zona Pelusida 3 (bZP3) terhadap Diameter dan Atresia Folikel Ovarium Mencit (Mus musculus)

Majalah Obstetri & Ginekologi ◽

10.20473/mog.v24i12016.37-42 ◽

2018 ◽

Vol 24 (1) ◽

pp. 37

Author(s):

Annisa Trissatharra ◽

Sri Ratna Dwiningsih ◽

Ratna Sofaria Munir

Keyword(s):

Mus Musculus ◽

Ovarian Follicles ◽

Observation Time ◽

Control Group ◽

Control Group Design ◽

Treatment Groups ◽

Group 4 ◽

Group 3 ◽

Group 2 ◽

Group 1

Objectives: To identify the effect of monoclonal antibody bZP3 at ovarian follicles that undergo atresia and diameter of various ovarian follicles.Materials and Methods: This is a true experimental research with post only control group design. Samples were 36 female mices (Mus musculus) which is divided into 6 groups, there are 3 control groups (group 1, 2, and 3) injected by Phospatase Buffer Saline (PBS) 50µl and 3 treatment groups (group 4, 5, and 6) injected by Mab bZP3 50µl. Group 1 and 4 terminated at 5th day, group 2 and 5 terminated at 10th day, and group 3 and 6 terminated at 20th day. Evaluation of atretic ovarian follicles and diameter of ovarian follicles performed by hematoxylin eosin (HE) and the data processed by parametric statistic.Results: There are no significant in different among groups in the aspect of atretic follicles and diameter of folicles (p>0.05), but descriptively, number of follicles undergo atresia of the follicle primary, secondary, and tertiary treatment group was higher than the control group, except on the 20th day of observation time.Conclusion: administration of Mab bZP3 had no effect to amount of atretic follicles and diameter of folicles during observation time.

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Zingerone Ameliorates Hepatotoxicity

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i1.1817 ◽

2020 ◽

Vol 11 (1) ◽

pp. 280-284 ◽

Cited By ~ 1

Author(s):

Julietpoornamathy J ◽

Parameswari C.S.

Keyword(s):

The Body ◽

Control Group ◽

Medical Sciences ◽

Toxic Compounds ◽

Group 4 ◽

Group 3 ◽

Group 5 ◽

Group 2 ◽

Insight Into ◽

Group 1

In medical sciences, toxicity is an area wherein extensive studies have been carried to improve the diseases as well as to prevent. So, there is a high requirement for novel and improved alternative therapeutic strategies to manage diseases. The liver is the largest gland in the body, which executes several important mechanisms; it stores minerals and vitamins and releases them in periods of need. The main aim of this study was to give a closer insight into potent non- toxic compounds that is capable of modifying the responses. Animals were divided into five equal groups viz control (Group 1), administered with food and water ad libitum, (Group 2) administered with olive oil, (Group 3) administered with zingerone, (Group 4) administered with concanavalin A, (Group 5) administered with cyclosporine A followed by zingerone. Our results revealed significant changes in liver marker enzymes and liver histology of zingerone treated rats when compared to control rats. A corollary, zingerone has no toxic effect on hepatocytes and was found to be safe at a dose of 10mg/kg b wt and also ameliorates hepatotoxicity.

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Comparative evaluation of postoperative pain intensity after single-visit and multiple-visit retreatment cases: a prospective randomized clinical trial

Brazilian Dental Science ◽

10.14295/bds.2018.v21i1.1515 ◽

2018 ◽

Vol 21 (1) ◽

pp. 26 ◽

Cited By ~ 3

Author(s):

Hatice Miray Uyan ◽

Keziban Olcay ◽

Mutlu Özcan

Keyword(s):

Postoperative Pain ◽

Repeated Measures ◽

Control Group ◽

Periapical Lesions ◽

Single Visit ◽

Group 4 ◽

Multiple Visit ◽

Significant Difference ◽

Group 3 ◽

Group 2

Objective: This study was designed to evaluate postoperative pain after endodontic retreatment. Material and Methods: Asymptomatic, multi-rooted molar & premolar teeth requiring retreatment with 2–5 mm periapical lesions were included. Seventy-eight teeth were randomly placed in four groups (n=20): single-visit (control, group 1), Ledermix (group 2), metronidazole, ciprofloxacin, minocycline mixture (group 3), calcium hydroxide (group 4). The postoperative pain was recorded using a VAS at 6, 12, 24, and 48 h after retreatment. Statistical evaluation was performed using Two-Way Repeated Measures ANOVA and Tukey test. Results: Mild pain occurred in 67.5%, moderate in 30%, and flare-ups in 2.5%, and there was a significant difference between the groups (p<0.01). Significantly lower postoperative pain was observed in TAP and CaOH2 groups(p<0.05). In the 6, 12, and 24 h intervals, there was a significant difference in the pain levels (p<0.05). Conclusion: TAP and CaOH2 are effective for reducing postoperative pain after retreatment.KeywordsIntracanal medicaments; Multiple-visit; Postoperative pain; Retreatment; Single-visit.

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