Characteristics of Dominant Species Transition between the Cyanobacterium Microcystis aeruginosa and the Diatom Cyclotella sp. According to the Changes in Nitrogen and Phosphorus Concentration and Its Mass Ratio

An unusual bloom of Ceratium furcoides is reported for a station of the Taquacetuba compartment of the Billings Reservoir. The appearance of this bloom is attributed to the mixing and turbulence of the water column that removed Ceratium cysts from the surface of the sediment and promoted conditions for the growth of this species in the region of mixing. Cold fronts approaching the Billings Reservoir are probably the cause of the mixing and bloom. Also turbulence induced by wind increased phosphorus concentration in the water column. Ceratium furcoides was the dominant species at station 1 where the nutrient concentrations of nitrogen and phosphorus were high. Ceratium spp. blooms may be a problem for water treatment and massive mortality can affect the dissolved oxygen of the water producing fish kill.

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Microalgae population dynamics growth with AnMBR effluent: effect of light and phosphorus concentration

Water Science & Technology ◽

10.2166/wst.2018.207 ◽

2018 ◽

Vol 77 (11) ◽

pp. 2566-2577 ◽

Cited By ~ 4

Author(s):

P. Sanchis-Perucho ◽

F. Duran ◽

R. Barat ◽

M. Pachés ◽

D. Aguado

Keyword(s):

Light Intensity ◽

Nutrient Removal ◽

Dominant Species ◽

Experimental Period ◽

Phosphorus Concentration ◽

Nitrogen And Phosphorus ◽

Microalgae Culture ◽

Nutrient Removal Efficiency ◽

Real Wastewater ◽

Effect Of Light

Abstract The aim of this study was to evaluate the effect of light intensity and phosphorus concentration on biomass growth and nutrient removal in a microalgae culture and their effect on their competition. The photobioreactor was continuously fed with the effluent from an anaerobic membrane bioreactor pilot plant treating real wastewater. Four experimental periods were carried out at different light intensities (36 and 52 μmol s−1 m−2) and phosphorus concentrations (around 6 and 15 mgP L−1). Four green algae – Scenedesmus, Chlorella, Monoraphidium and Chlamydomonas– and cyanobacterium were detected and quantified along whole experimental period. Chlorella was the dominant species when light intensity was at the lower level tested, and was competitively displaced by a mixed culture of Scenedesmus and Monoraphidium when light was increased. When phosphorus concentration in the photobioreactor was raised up to 15 mgP L−1, a growth of cyanobacterium became the dominant species in the culture. The highest nutrient removal efficiency (around 58.4 ± 15.8% and 96.1 ± 16.5% of nitrogen and phosphorus, respectively) was achieved at 52 μmol s−1 m−2 of light intensity and 6.02 mgP L−1 of phosphorus concentration, reaching about 674 ± 86 mg L−1 of volatile suspended solids. The results obtained reveal how the light intensity supplied and the phosphorus concentration available are relevant operational factors that determine the microalgae species that is able to predominate in a culture. Moreover, changes in microalgae predominance can be induced by changes in the growth medium produced by the own predominant species.

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Joint effects of five environmental factors on the growth of cyanobacterium Microcystis aeruginosa

Water Quality Research Journal ◽

10.2166/wcc.2018.255 ◽

2018 ◽

Vol 54 (2) ◽

pp. 79-87 ◽

Cited By ~ 1

Author(s):

Guikun Hu ◽

Qingtian Zhang

Keyword(s):

Environmental Factors ◽

Microcystis Aeruginosa ◽

Univariate Analysis ◽

Joint Analysis ◽

Phosphorus Concentration ◽

Physical Factors ◽

Nitrogen And Phosphorus ◽

High Illumination ◽

Alkaline Conditions ◽

High Concentrations

Abstract In many lakes and reservoirs, Microcystis aeruginosa is one of the dominant bloom species. Five environmental factors, including nutrients and physical factors, were selected to evaluate their effects and interactions on the growth of M. aeruginosa (FACHB-905) by joint analysis in a laboratory batch culture. The results indicated that all five factors affected the growth rate alone or in combination, and that their interactions were complex. This cyanobacterium strain preferred higher water temperature and alkaline conditions, while not requiring high illumination or high concentrations of nitrogen and phosphorus. Owing to these features the bloom of this cyanobacterium appears easily in nature. The form of nitrogen (nitrate or ammonium) also affected the assessment of M. aeruginosa bloom. The possibility of M. aeruginosa bloom would still exist even if the phosphorus concentration in the water column was very low. The result provided a good basis for the analysis and prediction of M. aeruginosa blooms in terms of environmental assessment, because the joint analysis of multiple factors would offer more valuable information than a univariate analysis. This article has been made Open Access thanks to the kind support of CAWQ/ACQE (https://www.cawq.ca).

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Influence of increased nutrient supply on Microcystis aeruginosa at cellular and proteomic levels

Aquatic Microbial Ecology ◽

10.3354/ame01939 ◽

2020 ◽

Vol 85 ◽

pp. 47-58

Author(s):

Y Jiang ◽

Y Liu

Keyword(s):

Microcystis Aeruginosa ◽

Regulatory Protein ◽

Acid Synthesis ◽

Nutrient Supply ◽

Nitrogen Supply ◽

Nitrogen And Phosphorus ◽

Comprehensive Description ◽

Amino Acid Synthesis ◽

High Nutrient ◽

Proteomic Responses

Various studies have observed that increased nutrient supply promotes the growth of bloom-forming cyanobacteria, but only a limited number of studies have investigated the influence of increased nutrient supply on bloom-forming cyanobacteria at the proteomic level. We investigated the cellular and proteomic responses of Microcystis aeruginosa to elevated nitrogen and phosphorus supply. Increased supply of both nutrients significantly promoted the growth of M. aeruginosa and the synthesis of chlorophyll a, protein, and microcystins. The release of microcystins and the synthesis of polysaccharides negatively correlated with the growth of M. aeruginosa under high nutrient levels. Overexpressed proteins related to photosynthesis, and amino acid synthesis, were responsible for the stimulatory effects of increased nutrient supply in M. aeruginosa. Increased nitrogen supply directly promoted cyanobacterial growth by inducing the overexpression of the cell division regulatory protein FtsZ. NtcA, that regulates gene transcription related to both nitrogen assimilation and microcystin synthesis, was overexpressed under the high nitrogen condition, which consequently induced overexpression of 2 microcystin synthetases (McyC and McyF) and promoted microcystin synthesis. Elevated nitrogen supply induced the overexpression of proteins involved in gas vesicle organization (GvpC and GvpW), which may increase the buoyancy of M. aeruginosa. Increased phosphorus level indirectly affected growth and the synthesis of cellular substances in M. aeruginosa through the mediation of differentially expressed proteins related to carbon and phosphorus metabolism. This study provides a comprehensive description of changes in the proteome of M. aeruginosa in response to an increased supply of 2 key nutrients.

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Method application of optimal multi-parameter analysis to assess the distribution of water masses as an example of CTD data of the Barents Sea in summer 2017

Hydrosphere Еcology (Экология гидросферы) ◽

10.33624/2587-9367-2019-2(4)-38-51 ◽

2019 ◽

pp. 38-51

Author(s):

Valeriy G. Yakubenko ◽

Anna L. Chultsova

Keyword(s):

Barents Sea ◽

Field Measurements ◽

Structural Similarity ◽

Water Masses ◽

Parameter Analysis ◽

Water Dynamics ◽

Phosphorus Concentration ◽

Nitrogen And Phosphorus ◽

The Barents Sea ◽

Expert Assessments

Identification of water masses in areas with complex water dynamics is a complex task, which is usually solved by the method of expert assessments. In this paper, it is proposed to use a formal procedure based on the application of the method of optimal multiparametric analysis (OMP analysis). The data of field measurements obtained in the 68th cruise of the R/V “Academician Mstislav Keldysh” in the summer of 2017 in the Barents Sea on the distribution of temperature, salinity, oxygen, silicates, nitrogen, and phosphorus concentration are used as a data for research. A comparison of the results with data on the distribution of water masses in literature based on expert assessments (Oziel et al., 2017), allows us to conclude about their close structural similarity. Some differences are related to spatial and temporal shifts of measurements. This indicates the feasibility of using the OMP analysis technique in oceanological studies to obtain quantitative data on the spatial distribution of different water masses.

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Nitrogen and phosphorus in the Vistula River, Poland -- changes from source to mouth

Water Science & Technology ◽

10.2166/wst.1994.0236 ◽

1994 ◽

Vol 30 (5) ◽

pp. 177-186 ◽

Cited By ~ 4

Author(s):

Karin Sundblad ◽

Andrzej Tonderski ◽

Jacek Rulewski

Keyword(s):

Central Region ◽

Northern Region ◽

Point Sources ◽

Nutrient Loss ◽

Phosphorus Concentration ◽

Nitrogen And Phosphorus ◽

Concentration Data ◽

Diffuse Sources ◽

Vistula River ◽

South Central

Nitrogen and phosphorus concentration data representing samples collected once a month for nine months at 13 locations along the Vistula River are considered in a preliminary discussion of the sources of the nutrients transported to the Baltic Sea. Concentrations in relation to flow data indicated substantial differences between subbasins. Based on those differences, on the area-specific nutrient loss for a six-month period and on the wastewater discharge in each subbasin, four regions could be recognized in the river basin: i) the southern region with a large impact of point sources, ii) the south central region, where diffuse sources seemed to be of major importance, iii) the north central region with a combined effect of point and diffuse sources, and retention in two reservoirs, iv) the northern region where point sources seemed to be the dominating source, at least for phosphorus. Our results illustrate the importance of differences in phosphorus retention between the basins. Long-term retention along the course of the river, particularly in the two reservoirs, must be estimated to allow proper source apportionment in the Vistula basin. Concentration decreases in the Wloclawek Reservoir varied between 44 and 68% for P, and 11 to 37% for N, in the months with significant retention. In some months, however, concentrations increased, indicating a release of nutrients.

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Retrieval and Evaluation of Chlorophyll-a Concentration in Reservoirs with Main Water Supply Function in Beijing, China, Based on Landsat Satellite Images

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18094419 ◽

2021 ◽

Vol 18 (9) ◽

pp. 4419

Author(s):

Yuequn Lai ◽

Jing Zhang ◽

Yongyu Song ◽

Zhaoning Gong

Keyword(s):

Remote Sensing ◽

Water Supply ◽

Chlorophyll A ◽

Phosphorus Concentration ◽

Landsat 8 ◽

Supply Function ◽

Estimation Model ◽

Nitrogen And Phosphorus ◽

Chlorophyll A Concentration ◽

Guanting Reservoir

Remote sensing retrieval is an important technology for studying water eutrophication. In this study, Guanting Reservoir with the main water supply function of Beijing was selected as the research object. Based on the measured data in 2016, 2017, and 2019, and Landsat-8 remote sensing images, the concentration and distribution of chlorophyll-a in the Guanting Reservoir were inversed. We analyzed the changes in chlorophyll-a concentration of the reservoir in Beijing and the reasons and effects. Although the concentration of chlorophyll-a in the Guanting Reservoir decreased gradually, it may still increase. The amount and stability of water storage, chlorophyll-a concentration of the supply water, and nitrogen and phosphorus concentration change are important factors affecting the chlorophyll-a concentration of the reservoir. We also found a strong correlation between the pixel values of adjacent reservoirs in the same image, so the chlorophyll-a estimation model can be applied to each other.

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Feedback Regulation between Aquatic Microorganisms and the Bloom-Forming Cyanobacterium Microcystis aeruginosa

Applied and Environmental Microbiology ◽

10.1128/aem.01362-19 ◽

2019 ◽

Vol 85 (21) ◽

Cited By ~ 9

Author(s):

Meng Zhang ◽

Tao Lu ◽

Hans W. Paerl ◽

Yiling Chen ◽

Zhenyan Zhang ◽

...

Keyword(s):

Energy Metabolism ◽

Microcystis Aeruginosa ◽

Lake Taihu ◽

Inorganic Nitrogen ◽

Microbial Community Composition ◽

Nitrogen And Phosphorus ◽

Content Type ◽

Coculture System ◽

Eukaryotic Microorganisms ◽

Aquatic Microorganisms

ABSTRACT The frequency and intensity of cyanobacterial blooms are increasing worldwide. Interactions between toxic cyanobacteria and aquatic microorganisms need to be critically evaluated to understand microbial drivers and modulators of the blooms. In this study, we applied 16S/18S rRNA gene sequencing and metabolomics analyses to measure the microbial community composition and metabolic responses of the cyanobacterium Microcystis aeruginosa in a coculture system receiving dissolved inorganic nitrogen and phosphorus (DIP) close to representative concentrations in Lake Taihu, China. M. aeruginosa secreted alkaline phosphatase using a DIP source produced by moribund and decaying microorganisms when the P source was insufficient. During this process, M. aeruginosa accumulated several intermediates in energy metabolism pathways to provide energy for sustained high growth rates and increased intracellular sugars to enhance its competitive capacity and ability to defend itself against microbial attack. It also produced a variety of toxic substances, including microcystins, to inhibit metabolite formation via energy metabolism pathways of aquatic microorganisms, leading to a negative effect on bacterial and eukaryotic microbial richness and diversity. Overall, compared with the monoculture system, the growth of M. aeruginosa was accelerated in coculture, while the growth of some cooccurring microorganisms was inhibited, with the diversity and richness of eukaryotic microorganisms being more negatively impacted than those of prokaryotic microorganisms. These findings provide valuable information for clarifying how M. aeruginosa can potentially modulate its associations with other microorganisms, with ramifications for its dominance in aquatic ecosystems. IMPORTANCE We measured the microbial community composition and metabolic responses of Microcystis aeruginosa in a microcosm coculture system receiving dissolved inorganic nitrogen and phosphorus (DIP) close to the average concentrations in Lake Taihu. In the coculture system, DIP is depleted and the growth and production of aquatic microorganisms can be stressed by a lack of DIP availability. M. aeruginosa could accelerate its growth via interactions with specific cooccurring microorganisms and the accumulation of several intermediates in energy metabolism-related pathways. Furthermore, M. aeruginosa can decrease the carbohydrate metabolism of cooccurring aquatic microorganisms and thus disrupt microbial activities in the coculture. This also had a negative effect on bacterial and eukaryotic microbial richness and diversity. Microcystin was capable of decreasing the biomass of total phytoplankton in aquatic microcosms. Overall, compared to the monoculture, the growth of total aquatic microorganisms is inhibited, with the diversity and richness of eukaryotic microorganisms being more negatively impacted than those of prokaryotic microorganisms. The only exception is M. aeruginosa in the coculture system, whose growth was accelerated.

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