scholarly journals A linkage of gene of resistance to a broomrape race G with microsatellite loci of a sunflower linedonor RGP1 of VNIIMK’s breeding

2021 ◽  
Vol 186 (2) ◽  
pp. 3-9
Author(s):  
S.Z. Guchetl ◽  
◽  
D.L. Savichenko ◽  
Keyword(s):  
Microsatellite Loci ◽  
Physical Map ◽  
Pcr Analysis ◽  
Sources Of Resistance ◽  
Resistant Varieties ◽  
Literary Sources ◽  
Ssr Loci ◽  
Environmentally Safe ◽  
Yield Formation ◽  
Sunflower Genome

Broomrape (Orobanche cumana Wallr.) is one of the main biotic factors limiting high sunflower yield formation. The most effective and environmentally safe method of protection is cultivation of resistant varieties and hybrids of sunflower. Development of resistant sunflower genotypes includes search and usage of sources of resistance in breeding process as well as accurate and productive procedures of material assessment. The purpose of the research is to analyze a linkage of a gene Or7 with microsatellite loci of the line-donor of resistance to broomrape race G from the VNIIMK’s collection. The objects of the research are the line RGP1 – a donor of resistance to broomrape race G and a susceptible to this race line VR 678 from the VNIIMK’s collection. Sunflower plants were crossed in field to produce F1. Also we conducted self-pollination of F1 plants to obtain F2 progeny. Plants were tested in a greenhouse in soil infected with seeds of broomrape race G using a method of early diagnostic. Sunflower DNA was extracted from the top leaves of the young sprouts of the vegetative plants. For PCR-analysis we used three SSR-primers demonstrated polymorphism in parental lines: ORS 683, ORS 1040, and ORS 1112. We tested joint inheritance of the gene Or7 and these loci, and inheritance between SSR-loci. An independent inheritance of the gene Or7 with DNA-loci ORS 683, ORS 1040, and ORS 1112, as well as SSR-loci between ORS 1040 and ORS 1112, ORS 1040 and ORS 683 was showed. Loci ORS683 – ORS 1112 are linked with a frequency of recombination of 0.27 ± 0.41 (27 cM). As a result of our research location of the gene Or7 in the nearest area to microsatellite loci ORS 683, ORS 1040, and ORS 1112 was excluded. Basing on studied literary sources and a representative sunflower genome HanXRQr2.0-SUNRISE we made a partial physical map LG3 for determination of an area for the further search of a localization of the Or7 and DNAmarkers co-segregating with this gene.

scholarly journals Search and analysis of nuclear sequences in sunflower genome associated with genes of resistance to bromrape (Orobanche cumana Wallr.) races

2020 ◽  
Vol 183 (3) ◽  
pp. 11-19
Author(s):  
D.L. Savichenko ◽  
◽  
S.Z. Guchetl ◽  
A.V. Golovatskaya ◽  
◽  
...  
Keyword(s):  
Physical Map ◽  
Pcr Analysis ◽  
Sequence Characterized Amplified Region ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Parental Lines ◽  
Sunflower Genome ◽  
Simple Sequence ◽  
Selection Of ◽  
Russian Research

Studying of sunflower lines resistant to broomrape race G, bred in V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK), Krasnodar showed the genetic control of resistance is monogenic with incomplete dominance. The purpose of the research was to seek and analyze molecular markers for the genes of resistance to broomrape race G using PCRmethods. In our research we used six developed in VNIIMK lines-donors resistant to broomrape race G: RGP1, RGP2, RGB, RGL1, RGL2, RGA, and susceptible lines VK 101, VK 678, VK 680. We extracted DNA from the leaves of young sunflower plants using STAB-buffer. For PCR-analysis we used 17 pairs of primers of two types: SCAR (sequence characterized amplified region) and SSR (simple sequence repeat). Conditions of amplification are as recommended by authors of markers with experimental selection of a temperature for primers hybridization. Within the research we balanced involved into work SSR- and 12 SCAR-loci using BLAST and a set of HanXRQr1.0. Due to these data we composed a physical map of loci location. After assessment of six resistant and three susceptible parental lines, seven markers demonstrated polymorphism by a length of locus DNA and two ones – by presence/absence of amplified fragment. For markers ORS 683 and ORS 1112 we observed a relation between loci polymorphism and susceptibility of parental lines to broomrape. This was certified with a presence of an allele of 364 n.p. length in ORS 683 and an absence of an allele of 375 n.p. length in ORS 1112. These markers were noted as primary ones for hybridological analysis in F1 и F2 with selected pairs for crossing. Additional markers will be selected individually using obtained data on loci polymorphism. Thus, as a result of the research we created a physical map for the further markers selection, found hybrid combinations and markers from the studied ones for hybridological analysis.

scholarly journals Search of the new SSR-loci of DNA for development of effective technology for soybean genotyping

2021 ◽  
Vol 188 (4) ◽  
pp. 18-24
Author(s):  
S.A. Ramazanova ◽  
◽  
V.G. Savichenko ◽  
E.G. Ustarkhanova ◽  
E.D. Loginova ◽  
...  
Keyword(s):  
Polymorphic Information Content ◽  
Dna Amplification ◽  
Pcr Analysis ◽  
Major Protein ◽  
Soybean Cultivars ◽  
Literary Sources ◽  
Effective Technology ◽  
Soybean Genotypes ◽  
Ssr Loci ◽  
Polymorphism Level

Soybean is the major protein-oil crop of a huge economic importance. Currently, to describe the new cultivars being applied for a patent there are used the modern methods based on an analysis of microsatellite (SSR) loci of DNA. The purposes of this work were a search of the new microsatellite markers to optimize the existing technology of soybean cultivars certification and identification as well as selection of conditions for PCR analysis and to test them on cultivars from the VIR’s collection. Seven microsatellite loci demonstrated the high polymorphism level on soybean cultivars and located in the different chromosomes were chosen in the literary sources and librarian data bases. The optimal temperatures for annealing were selected empirically for all the pairs of SSR-markers. The results of DNA amplification of 20 soybean genotypes showed all seven studied SSR-loci were polyallel. In general, we revealed 22 alleles that on average are 3.1 per a locus. The effective number of alleles Ne for the studied soybean genotypes varied from 1.69 to 2.27 and on average was equal to 2.01. An average meaning of an index of the polymorphic information content (PIC) was 0.50. All the investigated soybean samples have the unique sets of alleles by the studied loci. Seven approbated loci can be used in development of an effective technology for identification and certification of the soybean genotypes.

scholarly journals Controlling the southern root-knot nematode (Meloidogyne incognita Chitwood) with grafted and resistant pepper varieties

2010 ◽  
Vol 16 (2) ◽  
Author(s):  
Z. Mándoki
Keyword(s):  
Meloidogyne Incognita ◽  
Control Method ◽  
Environmentally Friendly ◽  
Bell Pepper ◽  
N Gene ◽  
Root Knot Nematode ◽  
Resistant Varieties ◽  
Environmentally Safe ◽  
Resistant Rootstock ◽  
Southern Root Knot Nematode

Newly bred resistant bell pepper varieties and those grafted onto resistant rootstock s were tested in soil severely infested with southern root-knot nematode [Meloidogy11e incognita (Kofoid and White) Chitwood] in unheated plastic house and compared to varieties on their own roots, in order to evaluate the efficiency of this environmentally friendly control method. 'Cinema F I ' carrying the N gene yielded significantly more than the two susceptible varieties. Varieties grafted onto resistant rootstocks outyielded those on their own roots although to different extent, which was not always significant. At the end of the vegetat ion period the roots of the rootstocks were undamaged and the roots of some resistant varieties were slightly infected. whereas the roots of susceptible varieties were severely damaged. According to our result  . both the use of resistant varieties and grafted plants offer an effective and environmentally safe way of controlling M. incognita.

SOURCES OF RESISTANCE IN BARLEY TO PYRENOPHORA TERES

1965 ◽  
Vol 45 (2) ◽  
pp. 189-193 ◽  
Author(s):  
K. W. Buchannon ◽  
W. C. McDonald
Keyword(s):  
North Dakota ◽  
Quality Characteristics ◽  
Seedling Stage ◽  
Malting Quality ◽  
Western Canada ◽  
Pyrenophora Teres ◽  
Net Blotch ◽  
Sources Of Resistance ◽  
Highly Pathogenic ◽  
Resistant Varieties

The reaction to infection by Pyrenophora teres Drechs., the incitant of net blotch of barley, was determined for 6,174 varieties in the U.S.D.A. World Barley Collection. Forty varieties, seventeen of them from Ethiopia, were resistant in the seedling stage to a highly pathogenic strain of the fungus prevalent in Western Canada and to composites of isolates from Manitoba, Saskatchewan, Alberta, Ontario, North Dakota, California, and Mexico. They were also resistant in the field at three locations in Western Canada. Agronomic and malting quality characteristics for the resistant varieties were also recorded.

scholarly journals Current Virulence of Pyrenophora teres on Barley in Western Australia

Plant Disease ◽  
2001 ◽  
Vol 85 (9) ◽  
pp. 960-966 ◽  
Author(s):  
Sanjiv Gupta ◽  
Robert Loughman
Keyword(s):  
Western Australia ◽  
Error Variance ◽  
Geographic Area ◽  
Australian Isolate ◽  
Pyrenophora Teres ◽  
Sources Of Resistance ◽  
Pathogenic Variation ◽  
Resistant Varieties ◽  
Significant Line ◽  
Spot Type

Studies on variation, occurrence, and distribution of virulence in Pyrenophora teres are helpful to identify effective sources of resistance that can be used for barley breeding in Western Australia. Seventy-nine isolates of Pyrenophora teres were collected from different barley fields of Western Australia in 1995-96. Seventy-four induced net type symptoms (P. teres f. teres) and five induced spot type symptoms (P. teres f. maculata). Net type isolate responses on 47 barley lines were similar to the range of responses induced by nine historical isolates collected in the region between 1975 and 1985. These net type isolates were classified into two distinct groups based on virulence to the cultivar Beecher. Isolates were further classified into eight groups based on minor pathogenic variation among the population. The virulence phenotype present in an eastern Australian isolate was not observed in any isolates collected from Western Australia. An analysis of variance on a subset of 12 net type isolates indicated a significant line × isolate interaction (P < 0.001), with the interaction term variance component four times larger than the error variance. Based on these studies, the virulence among net type isolates has remained stable in Western Australia for the last 19 years. Spot type isolates were collected from a wider geographic area than previously reported and varied in virulence based on response to barley line Herta. Variation in spot-type isolates is reported for the first time from the region. The results from this study are being used in the development of resistant varieties.

scholarly journals SSR analysis of modern Russian potato varieties using DNA samples of nomenclatural standards

2021 ◽  
Vol 3 (4) ◽  
pp. 77-96
Author(s):  
O. Yu. Antonova ◽  
N. S. Klimenko ◽  
D. A. Rybakov ◽  
N. A. Fomina ◽  
V. V. Zheltova ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Genetic Relationships ◽  
Plant Genetic Resources ◽  
Potato Cultivars ◽  
Cultivated Plants ◽  
Pcr Analysis ◽  
Ssr Analysis ◽  
Ssr Loci ◽  
High Level ◽  
Modern Russian

The N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) is developing new approaches to documentation of national cultivars, taking into account recommendations of the International Code of Nomenclature for Cultivated Plants in parallel with methods of genetic certification. The nomenclatural standard of a particular cultivar represented by a herbarium specimen can be used as a reference for verifying authenticity and uniformity of cultivar specimens obtained from various sources. The verification requires fast and reliable methods for cultivar genotyping. This paper presents protocols for modified methods of DNA extraction, PCR-analysis and SSR-genotyping, which allow potato cultivars identification without the use of expensive reagent kits. A set of ten chromosome-specific microsatellite markers was used to study polymorphisms in 66 modern Russian potato cultivars, as well as in 11 pre-cultivars and breeding clones, represented by nomenclatural standards and voucher specimens, respectively. This subset of 77 specimens has demonstrated a high level of polymorphism in ten studied microsatellite loci. The SSR analysis identified 73 alleles; 7.3 alleles per locus were observed on average, the number of which varied from 3 (STG0025 locus) to 11 (locus StI046). The PIC values varied from 0.544 (STG0025 locus) to 0.836 (StI046 locus). The alleles, unique for this subset, were found at six studied loci. The high level of polymorphism at the SSR loci made it possible to unambiguously identify almost every cultivar, with the exception of the expected coincidence of microsatellite profiles of two cultivars, which are somaclonal variants. Using an optimized set of eight microsatellite markers, the genetic relationships of modern Russian potato cultivars were studied.

scholarly journals Tomato hybrids with complex resistance for ground greenhouses

2021 ◽  
pp. 34-37
Author(s):  
Т.А. Терешонкова ◽  
Р.А. Багров ◽  
М.Г. Фомичева ◽  
Н.Ф. Тенькова ◽  
Е.В. Титова ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Frankliniella Occidentalis ◽  
Insect Pests ◽  
Western Flower Thrips ◽  
Field Resistance ◽  
Tuta Absoluta ◽  
Pcr Analysis ◽  
Sources Of Resistance ◽  
Tomato Crop ◽  
Breeding Material

На фоне усиливающейся инфекционной нагрузки на культуру томата, обусловленной появлением новых болезней и новых физиологических рас традиционных патогенов, возрастает значение создания и использования в производстве гетерозисных гибридов с комплексной устойчивостью. Возрастает вредоносность новых насекомых-вредителей культуры томата. По вредоносности выходят на первое место томатная минирующая моль (Tuta absoluta) и западный цветочный трипс (Frankliniella occidentalis), который помимо нанесения прямого ущерба культуре выступает как переносчик вирусных заболеваний, в том числе вируса бронзовости (TSVW). Начатая селекционная программа на полевую устойчивость томата к трипсу позволила в 2020–2021 годах выделить восемь источников устойчивости среди образцов различных товарных групп. В результате двадцатилетней селекции созданы коммерческие гибриды с различным набором генов устойчивости. В задачи современной селекции входят этапы оценки и отбора селекционного материала на наличие генов устойчивости в генотипах методами ПЦР-диагностики, а также контроль устойчивости путем испытания материала в условиях инфекционных фонов или искусственного заражения. Важный этап – контроль товарных партий семян на присутствие генов устойчивости. Результаты ПЦР-анализа коммерческих гибридов на четыре гена устойчивости в сочетании с испытанием полевой устойчивости к кладоспориозу на сильном многолетнем инфекционном фоне приведены в табличной форме. Результаты показывают, что практически во всех товарных группах есть гибриды с устойчивостью к кладоспориозу, фузариозному увяданию, вирусу томатной мозаики (ВТоМ) и галловой нематоде. Также была обнаружена относительная устойчивость к фитофторозу у гибрида F1Изящный (Ph2\Ph2). Сегодня проводится работа по расширению числа генов для ПЦР-анализа селекционного материала. Under conditions of the increasing infectious load on the tomato crop due to the emergence of new diseases and new physiological races of traditional pathogens, the importance of creating and growing heterotic hybrids with complex resistance is increasing. The harmfulness of new insect pests of tomato culture is also increasing. In terms of harmfulness, the tomato mining moth (Tuta absoluta) and the western flower thrips (Frankliniella occidentalis) take the first place, which, in addition to causing direct damage to the culture, is a carrier of viral diseases, including Tomato spotted wilt virus. The started breeding program for the field resistance of tomato to thrips made it possible for the period 2020–2021 to identify 8 sources of resistance among the samples of various commodity groups. As a result of 20 years of selection, commercial hybrids with different sets of resistance genes have been created. The goals of modern breeding include breeding material assessment and selection for the presence of resistance genes in genotypes by PCR diagnostics method, as well as control of resistance by testing the material under infectious backgrounds or artificial infection inoculation. Another important step is the control of commercial seed lots for the presence of resistance genes. The results of PCR analysis of commercial hybrids for 4 resistance genes in combination with the test of field resistance to Cladosporium fulvum under condition of strong long-term infectious background are given in tabular form. These results show that in almost all product groups there are hybrids with resistance to ToMV, in combination with other resistances, including the relative resistance to late blight in the F1 Iziyaschniy (Ph2\Ph2) hybrid. Currently, the progress is being made towards expanding the number of genes for PCR analysis of breeding material.

scholarly journals Breeding Approaches for Biotic Stress Resistance in Vegetables

2021 ◽  
Author(s):  
Ramakrishna Swarnapriya
Keyword(s):  
Environmental Pollution ◽  
Biotic Stress ◽  
Human Consumption ◽  
Breeding Methods ◽  
Sources Of Resistance ◽  
Biotic Stresses ◽  
Resistant Varieties ◽  
New Races ◽  
Safe Food ◽  
High Yielding Varieties

In vegetables the factors for biotic stress are pests, diseases and nematodes. The damages induced by these factors reflect highly on production, productivity and quality. Although application of pesticides/fungicides and nematicides has managed these stresses, excessive use of unsafe chemicals results in environmental pollution and leave residues in vegetables which are above threshold levels and also promote the development of new races/biotypes of pests and pathogens. Therefore vegetable improvement works concentrate on high yielding varieties with multiple resistance to these biotic stresses. For such studies, the knowledge on the genetic basis of resistance and plant-pest/pathogen interactions is necessary which will in turn improve the efficiency of the breeding programmes by introducing resistant genes and result in high-yielding genetically resistant cultivars. For the development of resistant varieties and pre-breed lines, information on sources of resistance is prerequisite and serve as a backbone in the breeding programme. Further, gene action responsible for the inheritance of characters helps in the choice of suitable breeding methods for the improvement of the crop. Work has been done by using the various breeding methods and resistant varieties have been bred and they offer the cheapest means of pest/disease/nematode control. Resistant varieties obviate the use of chemicals, thus reduce environmental pollution and facilitate safe food for human consumption.

scholarly journals Variabilidad patogénica de Colletotrichum lindemuthianum y resistencia en germoplasma de Phaseolus vulgaris L. de Ecuador

2018 ◽  
Vol 29 (1) ◽  
pp. 19 ◽  
Author(s):  
Diego Rodríguez-Ortega ◽  
Laura Vega-Jiménez ◽  
Ángel Rubén Murillo-Ilbay ◽  
Eduardo Peralta-Idrovo ◽  
Juan Carlos Rosas-Sotomayor
Keyword(s):  
Phaseolus Vulgaris ◽  
Resistance Genes ◽  
Durable Resistance ◽  
Colletotrichum Lindemuthianum ◽  
Phaseolus Vulgaris L ◽  
Sources Of Resistance ◽  
Improved Varieties ◽  
Physiological Races ◽  
Resistant Varieties ◽  
Pathogenic Variability

Anthracnose caused by Colletotrichum lindemuthianum is one of the most economically important diseases of bean (Phaseolus vulgaris L.) cultivation in Ecuador. The best control alternative is the use of resistant varieties. C. lindemuthianum presents great pathogenic variability, which hinders the development of varieties with a lasting resistance, therefore, the knowledge of the presence and distribution of the physiological races of the pathogen and the identification of resistance genes are key to developing varieties with broad and lasting resistance. The objective of this research was to determine the pathogenic variability of C. lindemuthianum and to evaluate the resistance of Ecuadorian bean germplasm. The research was carried out between 2013 and 2014. Seventeen isolates of C. lindemuthianum from northern central Ecuador were characterized by the inoculation of a group of twelve standard differential bean varieties. Among the analyzed samples, thirteen races were identified; five of those races had not been previously reported in the country. The differential G2333 (Co-42, Co-52 and Co-7) presented resistance to every characterized races in Ecuador. In addition, twenty - one improved varieties and elite bean lines were evaluated with sixteen of the seventeen isolates, three genotypes were identified (TB2, TB3 and INIAP 485 Urcuquí) with resistance to the mentioned isolates, which can be used as sources of resistance to Anthracnose. The identified sources of resistance in this study will allow to plan the development of bean varieties with broad and durable resistance to C. lindemuthianum.

scholarly journals Microsatellite analysis indicates the specific genetic basis of Czech bolting garlic

2014 ◽  
Vol 50 (No. 3) ◽  
pp. 226-234 ◽  
Author(s):  
J. Ovesná ◽  
L. Leišová-Svobodová ◽  
L. Kučera
Keyword(s):  
Cluster Analysis ◽  
Length Polymorphism ◽  
Microsatellite Loci ◽  
Genetic Basis ◽  
Allium Sativum ◽  
Microsatellite Analysis ◽  
Breeding Potential ◽  
Ssr Loci

Garlic, Allium sativum L., is a vegetable long used for culinary and medical purposes. A certain level of garlic quality is required by the local consumers, which is usually preserved by the varieties grown in that region. The aim was to establish an assay offering fast and inexpensive differentiation of garlic varieties. Length polymorphism of microsatellite loci (SSR, ILP markers) is often used in such a case. No assays have been described earlier. A set of SSR and newly used ILP markers has been assembled and verified. SSR loci ASM53, ASM072, ASA08 and ASA17 were the most polymorphic. Up to 18 alleles were scored per these loci. Monomorphic loci were identified, and excluded from the assay. The assay allows for the authenticity and confirmation of Czech garlic varieties. Moreover, a cluster analysis separated the Czech bolting varieties, indicating their specific genetic basis. The breeding potential of contemporary garlic varieties and lines is discussed.

Sign in / Sign up

Export Citation Format

Share Document