Bioactive Compounds Investigation From Methanol Bark Extract of Pterocarpus marsupium Using GC-MS Analysis

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Aruna L. Hugar ◽  
Amarvani P. Kanjikar ◽  
Ramesh L. Londonkar
Keyword(s):  
Methyl Ester ◽  
Bioactive Compounds ◽  
Acid Methyl Ester ◽  
Bark Extract ◽  
Therapeutic Effects ◽  
Octadecanoic Acid ◽  
New Novel ◽  
Ms Analysis ◽  
Novel Drug ◽  
Therapeutic Activities

Introduction: Medicinal plants still represent untapped sources of novel compounds with potential therapeutic effects for mitigating human maladies.Pterocarpus marsupium, commonly known as Bijasal, is a well-known drug in ayurvedic system of medicine. Materials and methods: The dried bark powder of P.marsupium was extracted by Soxhlet with methanol and analyzed by gas chromatography, while the mass spectra data of the compounds found in the extract was matched with the data available at National Institute of Standards and Technology (NIST) library. Results: The results of the GC-MS analysis provide different peaksdetermining the presence of 10 major phytocomponents, that are 1-Penten-3-one,1-(2,6,6-trimethyl-1-cyclohexen-1-yl)-; Geranyl isovalerate; 2-Pentadecanone,6, 10, 14-trimethyl;Cyclopropaneoctanal, 2-octyl-; Octadecanoic acid;10-Octadecanoic acid, methyl ester; Heptadecanoic acid, 16-methyl,-methyl ester; Phytol; 4,8,12,16-Tetramethyl heptadecan-4-olide; Dasycarpidan-1-methanol, acetate with different therapeutic activities. Conclusion: The presence of these bioactive compounds justifies that the Pterocarpus marsupiumcan offer a base for using this compound as herbal alternative for the synthesis and development of new drug to treat various infectious diseases. However, isolation of individual phytochemical constituents might be useful to formulate a new novel drug.

scholarly journals Bioactive Variability and In Vitro and In Vivo Antioxidant Activity of Unprocessed and Processed Flour of Nine Cultivars of Australian lupin Species: A Comprehensive Substantiation

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 282 ◽  
Author(s):  
Kishor Mazumder ◽  
Afia Nabila ◽  
Asma Aktar ◽  
Asgar Farahnaky
Keyword(s):  
Antioxidant Activity ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Acid Methyl Ester ◽  
Heat Processing ◽  
Acid Methyl ◽  
Ms Analysis

The aim of this present investigation was to analyze bioactive compounds, as well as demonstrate the antioxidant activities of nine cultivars of Australian lupin species accompanied by observing the effect of domestic heat processing on their antioxidant activities adopting in vivo and in vitro approaches. Gas chromatography mass spectroscopy (GC-MS) analysis was performed for profiling bioactive compounds present in lupin cultivars. Multiple assay techniques involving quantification of polyphenolics, flavonoids and flavonol, electron transfer (ET) based assay, hydrogen atom transfer (HAT)-based assay and in vivo assays were performed. The major compounds found were hexadecanoic acid methyl ester, 9,12-octadecadienoic acid methyl ester, methyl stearate, lupanine,13-docosenamide and 11-octadecenoic acid (Z)- methyl ester. Mandelup was found to show excellent antioxidant activity. Moreover, Jurien, Gunyidi and Barlock had strong antioxidant activity. Both positive and negative impacts of heat processing were observed on antioxidant activity. Heating and usage of excess water during processing were the key determinants of loss of antioxidants. Negligible loss of antioxidant activity was observed in most of the assays whereas inhibition of both lipid peroxidation (33.53%) and hemolysis of erythrocytes (37.75%) were increased after processing. In addition, in vitro and in vivo antioxidant assays are found to show statistically significant (* p < 0.05 and ** p < 0.01) results, which are supported by the presence of a number of antioxidant compounds in GC-MS analysis.

Identification of Potential Antimicrobial Compounds from A Marine Streptomyces Sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria

2021 ◽  
Vol 17 ◽  
Author(s):  
Nesrine Boublenza ◽  
Nadir Boudjlal Dergal ◽  
Larbi Belyagoubi ◽  
Noujoud Gabed ◽  
Djamel-Eddine Abdelouahid ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Acid Methyl Ester ◽  
Bioactive Metabolites ◽  
Streptomyces Sp ◽  
Acid Methyl ◽  
Ms Analysis ◽  
Western Algeria ◽  
Marine Streptomyces

Background: Marine actinobacteria are a potential resource for natural products; their secondary bioactive metabolites have shown several biological activities. Most of the isolated and identified actinobacteria in Algeria were usually explored from caves, Saharan soil or palm groves. The marine ecosystem is poorly explored and documented. Methods: Five Streptomyces strains producing bioactive compounds were isolated from Rachgoun Island located in Western Algeria and characterised phenotypically and genotypically using microbiological and 16S rRNA sequencing methods, respectively. The crude extract of the most representative strain “Streptomyces sp. strain SM2.4” and its seven active fractions were characterised by GC/MS analysis. Results: Streptomyces sp. strain SM2.4 revealed the strongest activity against the Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis, the fungus Aspergillus niger and was inactive against Gram-negative bacteria. GC/MS analysis of the methylated crude extract of Streptomyces sp. strain SM2.4 revealed the presence of 11 major compounds including fatty acids methyl ester (12-methyltridecanoic acid methyl ester, 9-hexadecenoic acid methyl ester, hexadecanoic acid methyl ester, 14-methylhexadecanoic acid methyl ester and 16-methylheptadecanoic acid methyl ester), 2,4-di-tert-butylphenol, (4S,4aS,8aR)-4,8a-dimethyloctahydro-4a(2H)-naphthalenol (geosmin), 2,4-dimethylbenzaldehyde, 3,4-difluorobenzaldehyde, dimethylfuran-2,4-dicarboxylate and pyrrolo(1,2-a)pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-. Partial purification of the crude extract by Thin-layer chromatography provided seven active fractions which were tested by radial diffusion assay. GC/MS analysis of the active TLC-fractions revealed the presence of a mixture of active compounds from which 2-(bromomethyl)-2-(2-methylphenyl)-1,3-dioxolane was found to be a new 1,3 dioxolane derivative. Furthermore, 3,4-dimethylbenzamide and pyrido[2,3-d] pyridazine-1,4-dione, hexahydro-3-(2-methylpropyl)-, were extracted for the first time from a natural source. Conclusion: Our study reveals that marine Streptomyces sp. strain SM2.4 has an interesting antimicrobial potential due to its panel of bioactive compounds.

scholarly journals GC-MS ANALYSIS AND ANTIMICROBIAL ACTIVITY (VITRO TEST) OF (Balanites aegyptiaca) ESSENTIAL OIL

2021 ◽  
pp. 93-96
Keyword(s):  
Methyl Ester ◽  
Octadecadienoic Acid ◽  
Acid Methyl Ester ◽  
Chemical Extraction ◽  
Octadecanoic Acid ◽  
Balanites Aegyptiaca ◽  
Acid Methyl ◽  
Ms Analysis ◽  
Vitro Test ◽  
Normal Hexane

The aim of the present study was to extract oil from Heglig seeds (Balanites aegyptiaca) to indicate phytochemical properties, GC/MS analysis and anti- biological activity (Vitro test). The kernel seeds were ground, and 250 g were transferred into a soxhelt unit and extracted by using normal hexane. The chemical extraction followed by GC-MS analysis result in 25 compounds, the compound which had higher percentage are: 9-Octadecanoic acid (Z)-methyl ester 29.46%, 9, 12-Octadecadienoic acid (Z, Z)-, methyl ester 29.18%, Methyl stearate 18.71%, Hexadecanoic acid, methyl ester 16.70%. and Eicosanoic acid, methyl ester 1.81%. The oil showed activity only against Candida Albicans.

scholarly journals GC-MS analysis of bioactive compounds in ethanolic leaf extract of Hellenia speciosa (J.Koenig) S.R.Dutta

2021 ◽  
Author(s):  
Ramya Raveendran
Keyword(s):  
Methyl Ester ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Acid Methyl Ester ◽  
Acid Methyl ◽  
Ms Analysis ◽  
The Family ◽  
Intestinal Worms ◽  
Phytochemical Components ◽  
Ethanolic Leaf Extract

Abstract Hellenia speciosa (J.Koenig) S.R.Dutta, is a plant species belonging to the family Costaceae. It is widely distributed in China, India, Malaysia, Indonesia, tropical and subtropical Asia. In Ayurveda, the rhizome of the plant has been extensively used to treat fever, rash, asthma, bronchitis and, intestinal worms. The objective of the present study was to investigate the phytochemical constituents of the leaf of Hellenia speciosa using Gas Chromatography and Mass Spectroscopy analysis (GC-MS). The GC-MS analysis revealed the presence of 17 phytochemical components in the ethanolic leaf extract of Hellenia speciosa. The prevailing bioactive compounds present in Hellenia speciosa were Thymol (RT-10.019;3.59%), Caryophyllene (RT-11.854:0.62%), Caryophyllene oxide (RT-13.919;1.34%), Artumerone (RT-14.795;1.35%), Hexadecanoic acid methyl ester (RT-17.536; 2.77%), 9,12-Octadecanoic acid methyl ester (RT-19.163;1.35%), Squalene (RT- 24.980;1.19%), Piperine (RT-25.745;3.11%), Beta Tocopherol (RT-26.681;2.88%) Vitamin E (RT- 27.290;2.64%), Progesterone (RT-29.608;3.18%), Caparratriene (RT-29.861;9.72%), and Testosterone (RT-30.73;5.81%). The compounds were identified by comparing their retention time and peak area with that of the literature and by interpretation of mass spectra. The results and findings of the present study suggest that the plant can be used as a valuable source in the field of herbal drug discovery. The presence of bioactive compounds justifies the use of plant leaves for treating various diseases with fewer side effects and recommended the plant of pharmaceutical importance. However, further studies are needed to undertake its bioactivity and toxicity profile.

A review on phytochemical and therapeutic potential of Iris germanica

2021 ◽  
Author(s):  
Bahareh Sadat Yousefsani ◽  
Motahareh Boozari ◽  
Kobra Shirani ◽  
Amirhossein Jamshidi ◽  
Majid Dadmehr
Keyword(s):  
Bioactive Compounds ◽  
Therapeutic Potential ◽  
Experimental Studies ◽  
Therapeutic Effects ◽  
Pharmacological Activities ◽  
Pharmacological Studies ◽  
Iris Germanica ◽  
Safety Studies ◽  
History Of ◽  
Therapeutic Activities

Abstract Objectives Iris germanica L. is a medicinal plant, which has a long history of uses, mainly in medieval Persia and many places worldwide for the management of a wide variety of diseases. In this study, we aimed to review ethnopharmacological applications in addition to phytochemical and pharmacological properties of I. germanica. Key findings Ethnomedical uses of I. germanica have been reported from many countries such as China, Pakistan, India, Iran and Turkey. The medicinal part of I. germanica is the rhizome and the roots. Based on phytochemical investigations, different bioactive compounds, including flavonoids, triterpenes, sterols, phenolics, ceramides and benzoquinones, have been identified in its medicinal parts. Current pharmacological studies represent that the plant possesses several biological and therapeutic effects, including neuroprotective, hypoglycaemic, hypolipidaemic, antimicrobial, antioxidant, antiproliferative, anti-inflammatory, antiplasmodial, antifungal, immunomodulatory, cytotoxic and antimutagenic effects. Summary Although the majority of preclinical studies reported various pharmacological activities of this plant, however, sufficient clinical trials are not currently available. Therefore, to draw a definitive conclusion about the efficacy and therapeutic activities of I. germanica and its bioactive compounds, further clinical and experimental studies are required. Moreover, it is necessary to focus on the pharmacokinetic and safety studies on the extracts of I. germanica.

Fatty Acid Composition of Seeds of Satureja thymbra and S. cuneifolia

2003 ◽  
Vol 58 (7-8) ◽  
pp. 502-504 ◽  
Author(s):  
Ahmet C. Gören ◽  
Gökhan Bilsel ◽  
Mehmet Altun ◽  
Fatih Satıl
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Methyl Esters ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Octadecatrienoic Acid ◽  
Octadecanoic Acid ◽  
Hexadecanoic Acid ◽  
Acid Methyl ◽  
Main Components

Abstract The chemical composition of fatty acid methyl esters (FAMEs) from seeds of S. thymbra and S. cuneifolia were analyzed by GC/MS. 7 FAMEs were identified from the seeds of S. thymbra mainly as 9-octadecenoic acid methyl ester (43.9%), hexadecanoic acid methyl ester (11.4%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (30.2%), and octadecanoic acid methyl ester (14.1%), while from the seed of S. cuneifolia 10 FAMEs were obtained with the main components, similar to S. thymbra. These were identified as 9-octadecenoic acid methyl ester (10.1%), hexadecanoic acid methyl ester (methyl palmitate, 34.6%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (6.3%) and octadecanoic acid methyl ester (1.8%).

scholarly journals GC-MS ANALYSIS PROFILE AND BIOACTIVE COMPONENTS OF FLOWERS OF Bergenia ciliata, A WEED OF ROCK CREVICES

2021 ◽  
Vol 27 (4) ◽  
pp. 527-535
Author(s):  
Malik F. H. Ferdosi ◽  
Iqra Haider Khan ◽  
Arshad Javaid ◽  
Hafiz M. Saeed ◽  
Ifrayeem Butt ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Octadecadienoic Acid ◽  
Acid Methyl Ester ◽  
Literature Survey ◽  
Pentadecanoic Acid ◽  
Bioactive Components ◽  
Hexadecanoic Acid ◽  
Acid Methyl ◽  
Ms Analysis ◽  
Hexanedioic Acid

Bergenia ciliata is a weed of hilly areas of Pakistan that generally grows in rock crevices. In the present study, flowers of this weeds were collected from Murree. The dried flowers were extracted in methanol and subjected to GC-MS analysis that showed 7 compounds in it. The predominant compound was hexanedioic acid, bis(2-ethylhexyl) ester (48.88%) followed by γ-sitosterol (22.56%). Moderately occurring compound was cyclohexane, 1,3,5-triphenyl- (12.87%). The remaining compounds namely n-hexadecanoic acid (4.97%), pentadecanoic acid, 14-methyl-, methyl ester (3.77%), 9,12-octadecadienoic acid, methyl ester, (Z,Z)-  (2.94%) and 9,12,15-octadecatrienoic acid, methyl ester, (Z,Z,Z)- (2.92%) were categorized as less abundant compounds. Literature survey indicated that these compounds possess antimicrobial, larvicidal, anti-inflammatory, anticancer and/or antidiabetic properties.

scholarly journals GC-MS Analysis of the Fatty Acid Methyl Ester in Japanese Quail Fat

2015 ◽  
Vol 10 (2) ◽  
pp. 54-57
Author(s):  
Ion Dragalin ◽  
Olga Morarescu ◽  
Maria Sedcenco ◽  
Radu Marin Rosca
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Japanese Quail ◽  
Acid Methyl Ester ◽  
Acid Methyl ◽  
Ms Analysis
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