scholarly journals Immunodiagnostic Significance of Anti-RA33 Autoantibodies in Saudi Patients with Rheumatoid Arthritis

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Jamil A. Al-Mughales
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Primary Objective ◽  
The Other ◽  
Healthy Controls ◽  
Connective Tissue Disorders ◽  
Predictive Values ◽  
Healthy Control ◽  
Controlled Clinical Study ◽  
Saudi Patients

The primary objective of this study was to evaluate and compare the immunodiagnostic significance and utility of anti-RA33 with anti-CCP, RF, and CRP in Saudi patients with rheumatoid arthritis.Methods. This was a prospective controlled clinical study conducted at King Abdul Aziz University Tertiary Medical Centre. The sera of 41 RA patients, 31 non-RA patients, and 29 healthy controls were collected. Anti-RA33 and anti-CCP were measured using commercially available ELISA principle kits. RF and CRP were measured using nephelometry.Results. Anti-RA33 antibodies had the lowest positive and negative predictive values and showed a sensitivity of 7.32% with 95.12% specificity. Of the other three markers (including anti-CCP antibodies, CRP, and RF), only anti-CCP showed specificity of 90.46% with sensitivity of 63.41% compared to non-RA patients + healthy control. There was a significant correlation with rheumatoid factor positivity with anti-CCP. With respect to CRP, a notable correlation was seen only with anti-RA33.Conclusion. Compared to rheumatoid factor, anti-CCP antibodies, and C-reactive proteins, the anti-RA33 autoantibodies seem to be not representing as an important additional immunodiagnostic marker in Saudi patients with established RA. RA33 may have more interest in early RA or less severe RA and other systemic connective tissue disorders.

Relationship of HLA-DRB1 Alleles and Rheumatoid Arthritis in West South of Iran

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Karim Mowla ◽  
Elham Rajaee M. D. ◽  
Mehrdad Dargahi-MalAmir M. D. ◽  
Neda Yousefinezhad ◽  
Maryam Jamali Hondori
Keyword(s):  
Rheumatoid Arthritis ◽  
Environmental Factors ◽  
Rheumatoid Factor ◽  
Dna Extraction ◽  
Wrist Joint ◽  
Blood Sampling ◽  
Healthy Controls ◽  
Relationship Of ◽  
Synovial Pannus

Background: Rheumatoid arthritis is a systemic multifactor disease that presented with symmetrical polyarthritis more preferably in small wrist joint and ankle. Synovial pannus cause destruction and deformities in joints. The main reason of this disease in unknown, but past researchesshowed that genetically factor play important role beside environmental factors in susceptibility to this entity. Method:100 patients with rheumatoid arthritis diagnosed upon ACR 2010 criteria enrolled study. 92 healthy patents also enrolled DNA studying. of both group was extracted through DNA extraction kits by blood sampling. HLA-DRB1 typing was done by PCR-SSP method. Results: There were no significant differences in HLADRB1 *04, HLADRB1*08 and HLADRB1*11 alleles presentation between patients and healthy controls. Only there were statically significant correlation between HLA-DRB1*08 and Rheumatoid factor positive patents. (P = 0.025).

Utility of Rheumatoid Factor in the Diagnosis of Juvenile Rheumatoid Arthritis

PEDIATRICS ◽  
1986 ◽  
Vol 78 (3) ◽  
pp. 480-484 ◽  
Author(s):  
Andrew H. Eichenfield ◽  
Balu H. Athreya ◽  
Robert A. Doughty ◽  
Randall D. Cebul
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Juvenile Rheumatoid Arthritis ◽  
Pediatric Rheumatology ◽  
Diagnostic Value ◽  
Clinical Settings ◽  
Older Children ◽  
Predictive Values ◽  
Negative Results ◽  
Outpatient Settings

Rheumatoid factor is commonly used by clinicians to assess children with possible juvenile rheumatoid arthritis. To assess its usefulness, we reviewed the case histories of patients in whom latex agglutinating rheumatoid factor was determined during 1981 to 1982 at our institution. A total of 437 charts were available for review. There were 11 patients with positive tests for rheumatoid factor, of whom five had juvenile rheumatoid arthritis, all polyarticular. A total of 426 children had negative results, of whom 100 had juvenile rheumatoid arthritis. This yields a sensitivity of 4.8% and a specificity of 98%. We then estimated the prevalence of juvenile rheumatoid arthritis in three clinical settings: a primary practitioner's office, a tertiary children's hospital walk-in clinic, and a pediatric rheumatology center. The predictive values and marginal benefits for rheumatoid factor were then calculated in those settings using Bayes' theorem. In the two general outpatient settings, the primary practitioner's office and tertiary walk-in clinic, the positive predictive values were 0.7% and 0.5%, respectively; marginal benefits were 0.4% and 0.3%, respectively. Rheumatoid factor testing appeared to be of some benefit in the pediatric rheumatology center with a positive predictive value of 72.5% and marginal benefit of 22.5%. In no case was rheumatoid factor testing helpful in establishing a diagnosis of juvenile rheumatoid arthritis or in ruling it out. Testing for rheumatoid factor is a poor screening procedure for juvenile rheumatoid arthritis in the general situations in which it is more likely to be requested and of supportive diagnostic value only in the highly restricted population of older children with polyarticular arthritis.

scholarly journals CLINICAL EVALUATION OF VAITARANA BASTI ALONG WITH DHANWANTARA TAILA MATRA BASTI IN AMAVATA: A CASE SERIES

2020 ◽  
Vol 11 (6) ◽  
pp. 12-15
Author(s):  
Sheetal G Lodha ◽  
Ruchika S Karade
Keyword(s):  
Rheumatoid Arthritis ◽  
Connective Tissue ◽  
Rheumatoid Factor ◽  
Morning Stiffness ◽  
Dominant Role ◽  
Case Series ◽  
Signs And Symptoms ◽  
The Other ◽  
Knee Joints ◽  
The Common

Amavata is one of the common and most crippling joint disorders. It is a chronic, degenerative disease of the connective tissue mainly involving the joints. The clinical features of Amavata such as pain, swelling and stiffness of joints, fever and general disability are very much close to the Rheumatological disorder called rheumatoid arthritis. Ama associated with aggravated vata plays a dominant role in the pathogenesis of Amavata. According to its pathophysiology, one should treat the morbid doshas involve in are kapha and vata simultaneously. In the present study, four clinically diagnosed cases of Amavata with swelling of knee joints and morning stiffness , pain in multiple joints, raised rheumatoid factor and anti CCP factor are treated with Vaitarana basti along with Dhanwantara taila Matra basti on same day and changes are observed in subjective and objective criteria. Significant improvement is observed in reducing signs and symptoms of Amavata and in rheumatoid arthritis factor and anti CCP. Vaitarana basti eradicate Ama and kapha dosha as the drugs of Vaitarana basti having Ama pachaka, vatakapha shamaka and Anulomaka properties. On the other hand, Matra basti of Dhanwantara taila pacifies the vatadosha and reduced the pain and swelling. It also acts as neuroprotective, analgesic, anti-inflammatory, anti-arthritic and anti-paralytic. The combination of Vaitarana basti and Dhanwantara taila Matra basti can be an effective treatment for Amavata.

AB0043 THE IMBALANCE OF T FOLLICULAR REGULATORY CELL AND T FOLLICULAR HELPER CELL IN RHEUMATOID ARTHRITIS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1324-1325
Author(s):  
R. Su ◽  
Y. Y. Wang ◽  
F. Y. Hu ◽  
X. Zheng ◽  
Y. Liu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Peripheral Blood ◽  
Control Group ◽  
Healthy Controls ◽  
Regulatory Cells ◽  
Regulatory Cell ◽  
Helper Cells ◽  
Follicular Helper ◽  
Healthy Control ◽  
T Follicular Helper Cell

Background:Rheumatoid arthritis (RA) is a chronic inflammatory disease which can lead to severe joint damage and disability.The relationship between antibodies and rheumatoid arthritis has long been well established. Recently, many studies have found that T follicular regulatory cells(Tfr) and T follicular helper cells (Tfh) are closely related to antibody generation on lymphoid follicular germinal centers (GCs)[1-2]. Tfr cells can inhibite the GC reaction and suppress production of high-affinity antibodies. The dysregulation of Tfh cells can lead to the production of autoantibodies by B cells.Objectives:To examine the expression of circulating T follicular regulatory cell (Tfr) and T follicular helper cell and its subsets(Tfh1 Tfh2 Tfh17) in RA patients and healthy control group.Methods:Level of Tfr and Tfh1,Tfh2 and Tfh17 cells in the peripheral blood of 17 new RA patients, 30 treated RA patients and 18 healthy controls were deceted by flow cytomery. All patients were hospitalised at the Department of Rheumatology, Second Hospital of Shanxi Medical University.Results:We found that the level of Tfr (CD3+CD4+CD25+CXCR5+FOP3+) percent(P=0.020), in the peripheral blood in RA patients were significantly decreased compared with healthy controls. The percent of Tfh (CD3+CD4+CXCR5+CD45RA-) (P=0.039)and Tfh17 (CD3+CD4+CXCR5+CD45RA-CXCR3-CCR6+) (P=0.000)were increased, but there are no statistical difference about Tfh1(CD3+CD4+CXCR5+CD45RA-CXCR3+CCR6-)(P=0.558) and Tfh2 (CD3+CD4+CXCR5+CD45RA-CXCR3-CCR6-) percent(P=0.079). We compared the above indicators between new and treated RA patients, and the results indicated that the Tfr(P=0.013),Tfh (P=0.002) and Tfh1(P=0.034) were significantly increased in the new RA patients compared to the treated RA patients, there were no differences between the two groups in Tfh2(P=0.419) and Tfh17 percent(P=0.124).Conclusion:Our results indicated that disorder of Tfr and Tfh subsets were involved in RA, restoring the Tfr/Tfh balance may be the potential therapeutic targets.Fig. 1.Comparison of Tfr, Tfh and its subsets(Tfh1 Tfh2 Tfh17) percent among the RA patients (n = 47) and healthy control group (n = 18) (*P < 0.05).Fig. 2.Comparison of Tfr,Tfh and its subsets(Tfh1 Tfh2 Tfh17) percent among the new RA patients (n = 17) and treated RA patients(n = 30) (*P < 0.05).References:[1]Deng J, Wei Y, Fonseca VR, Graca L, Yu D.T follicular helper cells and T follicular regulatory cells in rheumatic diseases[J].Nat Rev Rheumatol. 2019, 15(8):475-490.[2]Chen Liu, Dongwei Wang, Songsong Lu, et al.Increased Circulating Follicular Treg Cells Are Associated With Lower Levels of Autoantibodies in Patients With Rheumatoid Arthritis in Stable Remission.Arthritis Rheumatol. 2018, 70(5):711-721Disclosure of Interests:None declared

Emotion facial expression in families of patients with schizophrenia

2011 ◽  
Vol 26 (S2) ◽  
pp. 1399-1399
Author(s):  
S. Herrera ◽  
M. Bardón ◽  
C. Fernández ◽  
V. Ángeles ◽  
G. Lahera Forteza ◽  
...  
Keyword(s):  
Mental Health ◽  
Facial Expression ◽  
Healthy Volunteers ◽  
Emotion Recognition ◽  
The Other ◽  
Control Group ◽  
Healthy Controls ◽  
Basic Emotions ◽  
Healthy Control ◽  
Face Emotion Recognition

IntroductionPatients with schizophrenia show a deficit in emotion recognition through facial expression and the low sense of familiarity may be a factor involved. However, the emotion facial expression in families of patients could be disturbed and be another factor related to the deficit in emotion recognition and in sense of familiarity in schizophrenia.ObjectivesTo assess the emotion facial expression in a sample of 21 families of patients with schizophrenia and families of healthy controls.Methods22 healthy volunteers, all of them professionals of mental health, were assessed with the Ekman Test of emotion recognition in unfamiliar people which was photographed by expressing the 6 Ekman’s basic emotions. The task was composed of 42 pictures, half of them from families of patients and the other half from families of healthy control.ResultsVolunteers recognize worse emotions in relatives of patients than in relatives of control group and this difference was statistically significant (Wilcoxon W = -4.13; p = .001). The average of pictures correctly recognized from families of patients was lower than pictures from families of control group (54.28% vs. 82%).ConclusionsThe emotion facial expression in families of patients with schizophrenia seems worse than in families of healthy controls. It could be a factor involved in face emotion recognition deficit in schizophrenia.

HLA-DRβ2 and IgM Rheumatoid Factor (RF) Positive Rheumatoid Arthritis (RA)

1988 ◽  
Vol 1 (2) ◽  
pp. 119-122
Author(s):  
G. Haberhauer ◽  
P. Peichl
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Control Subject ◽  
Disease Susceptibility ◽  
Healthy Control Subject ◽  
Chain Gene ◽  
Igm Rheumatoid Factor ◽  
Healthy Control

48 patients suffering from classic erosive RA with conditions persisting for more than 6 years were examined together with 69 healthy control subject with regard to associations to supertypic HLA-D specificities. We found a significant increase of HLA-DRw53 in patients with RF positive RA (22/31 pat. = 71%,Chi2 = 13.2, p<0.01). Our results confirm the hypothesis that the HLA-DRβ2 chain gene is closer related to the disease susceptibility of seropositive RA than the DRβ1 chain gene (DR4).

scholarly journals Use of ICD-10 diagnosis codes to identify seropositive and seronegative rheumatoid arthritis when lab results are not available

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Jeffrey R. Curtis ◽  
Fenglong Xie ◽  
Hong Zhou ◽  
David Salchert ◽  
Huifeng Yun
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
High Sensitivity ◽  
Administrative Claims ◽  
Test Results ◽  
Predictive Values ◽  
Diagnosis Codes ◽  
Positive Predicted Value ◽  
Icd 10 ◽  
Lab Test

Abstract Background Rheumatoid factor (RF) and anti-cyclic citrullinated peptide (CCP) antibody tests are often measured at the time of rheumatoid arthritis (RA) diagnosis but may not be repeated and therefore not available in electronic health record (EHR) data; lab test results are unavailable in most administrative claims databases. ICD10 coding allows discrimination between rheumatoid factor positive (M05) (“seropositive”) and seronegative (M06) patients, but the validity of these codes has not been examined. Methods Using the ACR’s Rheumatology Informatics System for Effectiveness (RISE) EHR-based registry and U.S. MarketScan data where some patients have lab test results, we assembled two cohorts. Seropositive RA was defined having a M05 diagnosis code on the second rheumatologist encounter, M06 similarly identified seronegative RA, and RF and anti-CCP lab test results were the gold standard. We calculated sensitivity (Se) and positive predicted value (PPV) of the M05/M06 diagnosis codes. Results We identified 43,581 eligible RA patients (RISE) and 1185 (MarketScan) with RF or anti-CCP lab test results available. Using M05 as the proxy for seropositive RA, sensitivity = 0.76, PPV = 0.82 in RISE, and Se = 0.73, PPV = 0.84 in MarketScan. Results for M06 as a proxy for seronegative RA were comparable in RISE, albeit somewhat lower in MarketScan. Over 3 consecutive visits, approximately 90% of RA patients were coded consistently using either M05 or M06 at each visit. Conclusion Under ICD10, M05 and M06 diagnosis codes are reasonable proxies to identify seropositive and seronegative RA with high sensitivity and positive predictive values if lab test results are not available.

scholarly journals Soluble selectins and highly fucosylated ?1-antichymotrypsin in rheumatoid arthritis patients

2015 ◽  
Vol 84 (1) ◽  
pp. 34-40
Author(s):  
Anna Olewicz-Gawlik ◽  
Izabela Korczowska-Łącka ◽  
Paweł Hrycaj
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Acute Phase Proteins ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Male And Female ◽  
Leukocyte Extravasation

Introduction. Fucosylation of acute phase proteins and serum soluble selectin levels is increased in rheumatoid arthritis (RA) patients and can influence leukocyte extravasation. Aim. The aim of this study was to evaluate the concentration and fucosylation of ?1-antichymotrypsin (ACT) in relation to serum concentrations of soluble forms of selectins in RA patients. Material and methods. Serum samples of 70 RA patients and 30 healthy controls were examined using sandwich enzyme-linked immunosorbent assay (ELISA). Results. ACT-FR was significantly increased in RA patients when compared to healthy controls (p < 0.001) and significantly correlated with serum concentrations of rheumatoid factor (RF) and antibodies against cyclic citrullinated peptides (ACPA) (p = 0.006, p = 0.04, respectively). Moreover, we found significant correlations between the serum levels of soluble (s)P- and sE-selectin and ACT-FR (p = 0.008 and p = 0.03, respectively) only in male RA patients.Conclusions. Fucosylation of ACT differs between male and female RA patients and is related to sP- and sE-selectin levels only in men.

scholarly journals Cytotoxic T lymphocyte-Associated Antigen +49G Variant Confers Risk for Anti-CCP- and Rheumatoid Factor-Positive Type of Rheumatoid Arthritis Only in Combination with CT60∗G Allele

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Bernadett Farago ◽  
Peter Kisfali ◽  
Lili Magyari ◽  
Noemi Polgar ◽  
Bela Melegh
Keyword(s):  
Rheumatoid Arthritis ◽  
Regression Analysis ◽  
Rheumatoid Factor ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Healthy Controls ◽  
Predisposing Factor ◽  
Positive Type ◽  
Allelic Combination

Controversial observations have been published on the association of the cytotoxic T lymphocyte associated antigen gene's variants with rheumatoid arthritis (RA). After genotyping 428 patients and 230 matched controls, the prevalence of theCT60∗G allele was more frequent in RF- and/or anti-CCP-seropositive RApatients, compared to the healthy controls (P<.001). Regression analysis revealed that theCT60∗G allele is a possible predisposing factor for RA in these subgroups. No accumulation of the+49∗G allele was found among patients, and this variant was not found to correlate with RA. Assaying the possible genotype variations, the+49∗G-CT60∗G allelic combination was accumulated in seropositive RA-subtypes, and was associated with the risk of RA (OR=1.73,P=.001for the whole RA-population). Although the+49∗G allele did not mean a predisposition to RA alone, in combination withCT60∗G it, also conferred risk, suggesting that the+49A/Gvariant is associated with the risk of RA only in certain haplotypes.

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