scholarly journals Sperm a cell in distress: Yoga to the rescue

2021 ◽  
Vol 2 ◽  
pp. 3
Author(s):  
Vidhu Dhawan ◽  
Rajeev Kumar ◽  
Neena Malhotra ◽  
Vatsla Dadhwal ◽  
Dibakar Borthakur ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Sperm Quality ◽  
Reproductive Potential ◽  
Redox Status ◽  
Paternal Genome ◽  
Critical Determinant ◽  
Complex Cells ◽  
Paternal Factors ◽  
Increase Risk ◽  
A Cell

Sperm, one of the complex cells of biological inheritance, are not only considered as mere vectors of transmission of paternal genome at the time of fertilization but also to events post-fertilization. The contribution of sperm molecular factors as a critical determinant of optimal embryonic development and pregnancy outcomes has been brought to surface. Spermatozoa with derangements in redox status, nuclear and mitochondrial genomic integrity, and dysregulated gene expression may affect the fertility status of the male and may result in impaired embryonic development and increase risk of genetic and epigenetic diseases in offspring. The integration of yoga-based lifestyle (YBL) as a part of the modern lifestyle has been found to be beneficial in the management of the derangements in the male reproductive functions in the distressing issue of infertility and early pregnancy loss (EPL) patients. As infertility and EPL are issues with a strong psychosomatic component, yoga a mind body intervention may be a useful adjunctive therapy in the management of these cases and may not only improve the sperm quality but also positively impact the reproductive potential, embryogenesis, and the health of the progeny. This short communication intends to highlight the importance of paternal factors as a causal factor for infertility, EPL, and also the promising role of integration of YBL in the management of such disorders.

scholarly journals N-acetyl-L-cysteine Improves the Developmental Competence of Bovine Oocytes and Embryos Cultured In Vitro by Attenuating Oxidative Damage and Apoptosis

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 860
Author(s):  
Wu-Sheng Sun ◽  
Hoon Jang ◽  
Mi-Ryung Park ◽  
Keon Bong Oh ◽  
Haesun Lee ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Early Stage ◽  
Developmental Competence ◽  
Beneficial Effects ◽  
Developmental Rates ◽  
A Cell ◽  
Bovine Oocytes ◽  
Dose Dependent

Oxidative stress has been suggested to negatively affect oocyte and embryo quality and developmental competence, resulting in failure to reach full term. In this study, we investigated the effect of N-acetyl-L-cysteine (NAC), a cell-permeating antioxidant, on developmental competence and the quality of oocytes and embryos upon supplementation (0.1–10 mM) in maturation and culture medium in vitro using slaughterhouse-derived oocytes and embryos. The results show that treating oocytes with 1.0 mM NAC for 8 h during in vitro maturation attenuated the intracellular reactive oxygen species (ROS) (p < 0.05) and upregulated intracellular glutathione levels (p < 0.01) in oocytes. Interestingly, we found that NAC affects early embryonic development, not only in a dose-dependent, but also in a stage-specific, manner. Significantly (p < 0.05) decreased cleavage rates (90.25% vs. 81.46%) were observed during the early stage (days 0–2), while significantly (p < 0.05) increased developmental rates (38.20% vs. 44.46%) were observed during the later stage (from day 3) of embryonic development. In particular, NAC supplementation decreased the proportion of apoptotic blastomeres significantly (p < 0.05), resulting in enhanced hatching capability and developmental rates during the in vitro culture of embryos. Taken together, our results suggest that NAC supplementation has beneficial effects on bovine oocytes and embryos through the prevention of apoptosis and the elimination of oxygen free radicals during maturation and culture in vitro.

Modulation by Heparin of the Interaction of the A1 Domain of von Willebrand Factor With Glycoprotein Ib

Blood ◽  
1999 ◽  
Vol 94 (12) ◽  
pp. 4186-4194 ◽  
Author(s):  
Christelle Perrault ◽  
Nadine Ajzenberg ◽  
Paulette Legendre ◽  
Ghassem Rastegar-Lari ◽  
Dominique Meyer ◽  
...  
Keyword(s):  
Von Willebrand Factor ◽  
Cell Aggregation ◽  
Cell Aggregates ◽  
Critical Determinant ◽  
Amino Terminal ◽  
A Cell ◽  
A1 Domain ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Amino Terminal Fragment

Abstract The conformation of the A1 domain of von Willebrand factor (vWF) is a critical determinant of its interaction with the glycoprotein (GP) Ib/V/IX complex. To better define the regulatory mechanisms of vWF A1 domain binding to the GPIb/V/IX complex, we studied vWF-dependent aggregation properties of a cell line overexpressing the GPIb, GPIbβ, and GPIX subunits (CHO-GPIbβ/IX cells). We found that CHO-GPIbβ/IX cell aggregation required the presence of both soluble vWF and ristocetin. Ristocetin-induced CHO-GPIbβ/IX cell aggregation was completely inhibited by the recombinant VCL fragment of vWF that contains the A1 domain. Surprisingly, the substitution of heparin for ristocetin resulted in the formation of CHO-GPIbβ/IX cell aggregates. Using monoclonal antibodies blocking vWF interaction with GPIb/V/IX or mocarhagin, a venom metalloproteinase that removes the amino-terminal fragment of GPIb extending from aa 1 to 282, we demonstrated that both ristocetin- and heparin-induced aggregations involved an interaction between the A1 domain of vWF and the GPIb subunit of the GPIb/V/IX complex. The involvement of heparin in cell aggregation was also demonstrated after treatment of heparin with heparinase that abolished CHO-GPIbβ/IX cell aggregation. These results indicated that heparin was able to induce vWF-dependent CHO-GPIbβ/IX cell aggregation. In conclusion, we demonstrated that heparin is capable of positively modulating the vWF interaction with the GPIb/V/IX complex.

Reduction in Percoll volume increases recovery rate of sex-sorted semen of bulls without affecting sperm quality and early embryonic development

2018 ◽  
Vol 192 ◽  
pp. 146-153 ◽  
Author(s):  
Daniele Missio ◽  
Natália Picolli Folchini ◽  
Fabio Gallas Leivas ◽  
Cecília Isabel Inês Urquiza Machado Pavin ◽  
Hirya Fernandes Pinto ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Recovery Rate ◽  
Sperm Quality ◽  
Early Embryonic Development

Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum

Development ◽  
1994 ◽  
Vol 120 (7) ◽  
pp. 2065-2075 ◽  
Author(s):  
W. Wurst ◽  
A.B. Auerbach ◽  
A.L. Joyner
Keyword(s):  
Spinal Cord ◽  
Embryonic Development ◽  
Normal Development ◽  
Cranial Nerves ◽  
Mutant Mice ◽  
Mouse Development ◽  
Developmental Defects ◽  
Targeted Deletion ◽  
The Third ◽  
A Cell

During mouse development, the homeobox-containing gene En-1 is specifically expressed across the mid-hindbrain junction, the ventral ectoderm of the limb buds, and in regions of the hindbrain, spinal cord, somites and somite-derived tissues. To address the function of En-1 during embryogenesis, we have generated mice homozygous for a targeted deletion of the En-1 homeobox. En-1 mutant mice died shortly after birth and exhibited multiple developmental defects. In the brains of newborn mutants, most of the colliculi and cerebellum were missing and the third and fourth cranial nerves were absent. A deletion of midhindbrain tissue was observed as early as 9.5 days of embryonic development and the phenotype resembles that previously reported for Wnt-1 mutant mice. In addition, patterning of the forelimb paws and sternum was disrupted, and the 13th ribs were truncated. The results of these studies suggest a cell autonomous role for En-1 in generation and/or survival of mid-hindbrain precursor cells and also a non-cell autonomous role in signalling normal development of the limbs and possibly sternum.

scholarly journals Effects of Heat Stress on the Redox Status in the Oviduct and Early Embryonic Development in Mice

2005 ◽  
Vol 51 (2) ◽  
pp. 281-287 ◽  
Author(s):  
Takaya MATSUZUKA ◽  
Manabu OZAWA ◽  
Ayako NAKAMURA ◽  
Atsuko USHITANI ◽  
Miho HIRABAYASHI ◽  
...  
Keyword(s):  
Heat Stress ◽  
Embryonic Development ◽  
Redox Status ◽  
Early Embryonic Development

Paternal factors and embryonic development: Role in recurrent pregnancy loss

Andrologia ◽  
2018 ◽  
Vol 51 (1) ◽  
pp. e13171 ◽  
Author(s):  
Vidhu Dhawan ◽  
Manoj Kumar ◽  
Dipika Deka ◽  
Neena Malhotra ◽  
Neeta Singh ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Paternal Factors

scholarly journals Social dominance, but not parasite load, affects sperm quality and sperm redox status in house sparrows

2019 ◽  
Vol 222 (17) ◽  
pp. jeb200675
Author(s):  
Sylvain Losdat ◽  
Alfonso Rojas Mora ◽  
Caroline Bellut ◽  
Rémi Chargé ◽  
Valentina Falchi ◽  
...  
Keyword(s):  
Social Dominance ◽  
Sperm Quality ◽  
Parasite Load ◽  
Redox Status ◽  
House Sparrows

scholarly journals The Chromatin Remodeler LET-418/Mi2 is Required Cell Non-Autonomously for the Post-Embryonic Development of Caenorhabditis elegans

2018 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Makhabbat Saudenova ◽  
Chantal Wicky
Keyword(s):  
Growth Factor ◽  
Embryonic Development ◽  
Progenitor Cells ◽  
Growth Factor Signaling ◽  
Chromatin Protein ◽  
C Elegans ◽  
Blast Cells ◽  
A Cell ◽  
Further Development ◽  
Growth Factor Signaling Pathway

Chromatin condition is crucial for the cells to respond to their environment. In C. elegans, post-embryonic development is accompanied by the exit of progenitor cells from quiescence in response to food. The chromatin protein LET-418/Mi2 is required for this transition in development indicating that proper chromatin structure in cells of the freshly hatched larvae is important to respond to food. However, the identity of the tissue or cells where LET-418/Mi2 is required, as well as the developmental signals that it is modulating have not been elucidated. By restoring the activity of LET-418/Mi2 in specific tissues, we demonstrate that its activity in the intestine and the hypodermis is able to promote in a cell non-autonomous manner the exit of blast cells from quiescence and further development. Furthermore, we identify the IIS (insulin/insulin-like growth factor signaling) pathway to be one of the signaling pathways that is conveying LET-418/Mi2 cell non-autonomous effect on development.

Modulation by Heparin of the Interaction of the A1 Domain of von Willebrand Factor With Glycoprotein Ib

Blood ◽  
1999 ◽  
Vol 94 (12) ◽  
pp. 4186-4194
Author(s):  
Christelle Perrault ◽  
Nadine Ajzenberg ◽  
Paulette Legendre ◽  
Ghassem Rastegar-Lari ◽  
Dominique Meyer ◽  
...  
Keyword(s):  
Von Willebrand Factor ◽  
Cell Aggregation ◽  
Cell Aggregates ◽  
Critical Determinant ◽  
Amino Terminal ◽  
A Cell ◽  
A1 Domain ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Amino Terminal Fragment

The conformation of the A1 domain of von Willebrand factor (vWF) is a critical determinant of its interaction with the glycoprotein (GP) Ib/V/IX complex. To better define the regulatory mechanisms of vWF A1 domain binding to the GPIb/V/IX complex, we studied vWF-dependent aggregation properties of a cell line overexpressing the GPIb, GPIbβ, and GPIX subunits (CHO-GPIbβ/IX cells). We found that CHO-GPIbβ/IX cell aggregation required the presence of both soluble vWF and ristocetin. Ristocetin-induced CHO-GPIbβ/IX cell aggregation was completely inhibited by the recombinant VCL fragment of vWF that contains the A1 domain. Surprisingly, the substitution of heparin for ristocetin resulted in the formation of CHO-GPIbβ/IX cell aggregates. Using monoclonal antibodies blocking vWF interaction with GPIb/V/IX or mocarhagin, a venom metalloproteinase that removes the amino-terminal fragment of GPIb extending from aa 1 to 282, we demonstrated that both ristocetin- and heparin-induced aggregations involved an interaction between the A1 domain of vWF and the GPIb subunit of the GPIb/V/IX complex. The involvement of heparin in cell aggregation was also demonstrated after treatment of heparin with heparinase that abolished CHO-GPIbβ/IX cell aggregation. These results indicated that heparin was able to induce vWF-dependent CHO-GPIbβ/IX cell aggregation. In conclusion, we demonstrated that heparin is capable of positively modulating the vWF interaction with the GPIb/V/IX complex.

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