Effect of Propionibacterium spp. on ruminal fermentation, nutrient digestibility, and methane emissions in beef heifers fed a high-forage diet1

2014 ◽  
Vol 92 (5) ◽  
pp. 2192-2201 ◽  
Author(s):  
D. Vyas ◽  
E. J. McGeough ◽  
S. M. McGinn ◽  
T. A. McAllister ◽  
K. A. Beauchemin
animal ◽  
2014 ◽  
Vol 8 (11) ◽  
pp. 1807-1815 ◽  
Author(s):  
D. Vyas ◽  
E.J. McGeough ◽  
R. Mohammed ◽  
S.M. McGinn ◽  
T.A. McAllister ◽  
...  

2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 181-182
Author(s):  
S Terry ◽  
G Ribeiro ◽  
R Gruninger ◽  
M Hünerberg ◽  
P Sheng ◽  
...  

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 419-420
Author(s):  
Tao Ran ◽  
Atef Saleem ◽  
YiZhao Shen ◽  
Gabriel Ribeiro ◽  
Adrian Tsang ◽  
...  

Abstract The objective of this study was to investigate the effects of a recombinant fibrolytic enzyme (RFE; xylanase XYL10C) on ruminal pH and fermentation characteristics, total tract digestibility and nitrogen balance in beef heifers. The experiment was a cross-over design with two treatments and two periods, using eight ruminal cannulated beef heifers. The treatments were a basal diet containing 60% barley silage, 30% barley straw and 10% supplement (DM basis) without (control) or with RFE. The RFE XYL10C was selected specifically for ruminants using a high throughput in vitro micro assay and was sprayed onto the barley straw at a rate of 6.6 × 104 IU/kg DM 24 h before feeding. The diet was prepared daily using a data ranger. Each period was 21 d, with a 14-d adaptation and 7-d sample collection period. The digestibility and nitrogen balance were measured by total fecal and urine collection. Data were analyzed using the PROC MIXED procedure of SAS with fixed effects of treatment, and random effects of period and heifer. Feed intake (8.9 kg/d) and total tract digestibility of DM (65.5%), NDF (57.2%) and ADF (51.2%) were not affected by RFE. Ruminal pH including mean (6.52), minimum (5.79), maximum (7.05), and duration of pH < 5.8 (0.64, h/d) did not differ between treatments. No treatment effects were observed for total VFA concentration (106 mM), molar proportion of acetate (68.2%) and propionate (17.5%) and acetate to propionate ratio (3.94). However, adding RFE vs. control tended (P < 0.08) to increase ruminal NH3-N concentration (5.75 vs. 5.23 mM) and endoglucanase activity (44.3 vs. 40.7 µmol glucose/min/mL wet rumen contents). There were no differences in nitrogen utilization and rumen microbial protein synthesis (90.7 g/d) between treatments. The results indicate that RFE XYL10C did not improve fiber digestion in heifers fed a high forage diet.


2019 ◽  
Vol 71 (6) ◽  
pp. 2065-2074 ◽  
Author(s):  
A.D.B. Ribeiro ◽  
M.V.C. Ferraz Junior ◽  
D.M. Polizel ◽  
A.A. Miszura ◽  
L.G.M. Gobato ◽  
...  

ABSTRACT Two experiments were conducted to evaluate the effect of thyme (Thymus vulgaris) essential oil (EO) doses on rumen fermentation, nutrient digestibility, and nitrogen metabolism, as well as performance and coccidia oocyst discharge. In experiment I, 20 rumen-cannulated wethers received the experimental diets containing 80% dry matter (DM) of haylage and 20% DM of concentrate. Treatments were 25mg of monensin/kg DM or doses of 1.25, 2.50, or 3.75g of thyme EO/kg DM. In experiment II, 50 ewe lambs received the same diets from experiment I, including a diet without feed additives. Wethers fed with diets containing 1.25g/kg DM of thyme EO had higher molar proportion of propionate (P= 0.03) and butyrate (P< 0.01), and lower (P= 0.04) acetate to propionate ratio than other treatments. Adding thyme EO to diets increased (P= 0.02) nitrogen retention compared to monensin. The performance of ewe lambs was not affected (P≥ 0.05) by treatments. However, lambs fed monensin had a lower (P= 0.04) number of coccidia oocyst discharge than others. Adding 1.25g/kg DM of thyme EO in high-forage diet improved ruminal fermentation. Thyme EO enhanced nitrogen metabolism, however, it did not improve performance.


Author(s):  
Brittney D Sutherland ◽  
Jordan A Johnson ◽  
John McKinnon ◽  
Tim A. McAllister ◽  
Gregory B. Penner

The objective of this study was to evaluate the use of barley (BS) or corn (CS) silage when fed with dry-rolled barley (BG), corn (CG), or an equal blend (BCG) of BG and CG for backgrounding cattle. Ruminally cannulated heifers (n=5) were assigned to an incomplete 6 × 6 Latin square design. Treatments contained either BS or CS in combination with BG, CG, or BCG. Samples were collected to determine DMI, ruminal fermentation, total-tract nutrient digestibility, and nitrogen balance. Interactions between cereal silage and cereal grain sources were detected for the molar proportions of propionate and butyrate where the magnitude of change with the type of cereal grain was increased in heifers fed CS relative to BS. Feeding CS increased estimated microbial N production (silage, P = 0.022) and fecal N excretion (silage, P = 0.042) over BS. Diets containing BG had greater DM, OM, starch, and GE digestibility values compared to CG, but values for BCG were not different (grain, P ≤ 0.043). Based on limited silage x grain interactions, use of CS in backgrounding diets may increase microbial N production relative to BS and dry-rolled CG may reduce apparent total-tract nutrient digestibility relative to dry-rolled BG.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 325-325
Author(s):  
Allison Stevens ◽  
Cheyanne Myers ◽  
John B Hall ◽  
Gwinyai E Chibisa

Abstract This study evaluated the interaction between the stage of maturity of Eragrostis tef (teff) grass and supplemental energy source on ruminal fermentation characteristics and nitrogen (N) utilization. Six ruminally-fistulated beef heifers were used in a 3 × 3 split-plot design (21 d periods). The whole plot factor was stage of maturity of teff hay [early- (EH) or late-heading (LH)], and the subplot factor was supplemental energy source [no supplement (CON), rolled corn grain or beet pulp pellet (BP)] fed at 0.5% of body weight. Feed intake was measured daily. Indwelling pH loggers were used to measure ruminal pH (d 14 to 21) and ruminal fluid was collected from d 19 to 21 to determine fermentation characteristics, as were feces and urine to measure N excretion. Data was analyzed using PROC MIXED in SAS. There was no stage of maturity × supplement interaction for all measurements (P ≥ 0.17). Although dry matter intake (DMI) tended to be greater (P = 0.07) for heifers fed EH than LH hay, it did not differ (P = 0.88) across supplements. Ruminal acetate concentration tended (P = 0.098) to be greater for EH than LH heifers, whereas butyrate, isobutyrate, and isovalerate concentrations were greater (P ≤ 0.045) for BPP than CON heifers. However, the duration and area pH &lt; 6.2 and 5.8 did not differ (P &gt; 0.12) across treatments. Except for microbial N flow, which was greater (P &lt; 0.01) for EH than LH heifers, there was no stage of maturity or supplement effect (P ≥ 0.14) on measures of N utilization. In summary, feeding teff hay harvested at the EH than LH stage of maturity increased DMI and microbial N supply, whereas feeding corn and BP as energy supplements to beef heifers had no effect on ruminal pH.


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