scholarly journals Lysine bioavailability among 2 lipid-coated lysine products after exposure to silage1

2017 ◽  
Vol 1 (3) ◽  
pp. 311-319 ◽  
Author(s):  
J. N. Reiners ◽  
J. E. Held ◽  
C. L. Wright ◽  
Q. Qiao ◽  
G. D. Djira ◽  
...  

Abstract We conducted 2 experiments to determine lysine bioavailability from 2 lipid-coated lysine products. In an in vitro experiment we mixed each lipid-coated lysine product with either alfalfa- or corn-silage at different amounts of acidity. Scanning electron micrographs indicated that surface structure of each lipid-coated lysine particle was eroded after mixing with silage. Additionally, visual evaluation of scanning electron micrographs suggested that peripheral surface abrasion of lipid-coated lysine may be greater when lipid-coated lysine was mixed with alfalfa silage in comparison to corn silage. In a corresponding experiment, in vivo measures of lysine bioavailability to sheep from 2 lipid-coated lysine products and lysine-HCl were determined after mixing in corn silage. Plasma lysine concentrations increased linearly (P < 0.01) in response to abomasal lysine infusion indicating that our model was sensitive to increases in metabolizable lysine flow. Bioavailability of each lipid-coated lysine source and dietary lysine-HCl were calculated to be 23, 15, and 18%, respectively. Even though each dietary source of lysine increased plasma lysine, rates of increases in plasma lysine from one lipid-coated lysine source (linear; P = 0.20) and lysine-HCl (linear; P = 0.11) were not different from plasma lysine levels supported by diet alone. However, the rate of plasma lysine increase in response to lysine from the other lipid-coated lysine source was greater (P = 0.04) than plasma lysine from feed alone. Nonetheless, the rate of plasma lysine increase in response to lipid-coated lysine did not differ (P ≥ 0.70) from the rate of plasma lysine increase from lysine-HCl. Clearly, methods of manufacture, together with physical and chemical characteristics of diet, can impact amounts of metabolizable lysine provided from lipid-coated lysine products. Direct measures of lysine bioavailability from lipid-coated lysine products after mixing with diets should be based on measurements with the products treated similarly to the method of feeding.

1974 ◽  
Vol 53 (6) ◽  
pp. 1368-1376 ◽  
Author(s):  
T.K. Huard ◽  
L.F. Arnold ◽  
P. Baram

Rhesus monkey periodontal ligament-derived fibroblasts were cultured on glass, Vitallium, poly(methyl methacrylate) and enzymatically debrided teeth. Scanning electron micrographs of these preparations and of the periodontal ligament surrounding normal and replanted teeth were compared. The fibroblasts cultured in vitro could organize on implant material and enzymatically debrided teeth to produce a network with fibers resembling those that are seen in the normal periodontal ligament.


2001 ◽  
Vol 8 (2) ◽  
pp. 320-324 ◽  
Author(s):  
Laura Plant ◽  
Patricia Conway

ABSTRACT Sixteen strains of Lactobacillus isolated from humans, mice, and food products were screened for their capacity to associate with Peyer's patches in mice. In preliminary experiments, in vitro binding to tissue pieces was assessed by scanning electron microscopy, and it was demonstrated qualitatively that 5 of the 16 strains showed some affinity for the Peyer's patches, irrespective of their association with the nonlymphoid intestinal tissue. Lactobacillus fermentum KLD was selected for further study, since, in addition to its intrinsically high adhesion rate, this organism was found to exhibit a preferential binding to the follicle-associated epithelium of the Peyer's patches compared with its level of binding to the mucus-secreting regions of the small intestine. Quantitative assessment of scanning electron micrographs of tissue sections which had been incubated with L. fermentum KLD or a nonbinding control strain, Lactobacillus delbruckii subsp.bulgaricus, supported these observations, since a marked difference in adhesion was noted (P < 0.05). This preferential association of strain KLD with the Peyer's patches was also confirmed with radiolabeled lactobacilli incubated with intestinal tissue in the in vitro adhesion assay. Direct recovery of L. fermentum KLD from washed tissue following oral dosing of mice revealed a distinct association (P < 0.05) between this organism and the Peyer's patch tissue. In contrast, L. delbruckii subsp. bulgaricus showed negligible binding to both tissue types in both in vitro and in vivo adhesion assays. It was concluded that L. fermentum KLD bound preferentially to Peyer's patches of BALB/c mice.


Development ◽  
1978 ◽  
Vol 48 (1) ◽  
pp. 185-203
Author(s):  
D. A. Bell ◽  
D. A. Ede

A method of culturing has been employed to compare the properties of cells migrating from small mesodermal explants taken from different regions of normal and mutant limb-buds at different stages of development. An analysis by time-lapse cinematography of the morphology and mobility of cells migrating from explants defines a distal region within the limb-bud where these properties are distinct from those of cells from more proximal regions. In the normal wing-bud distal cells subjacent to the apical ectodermal ridge possess a characteristic multipolar morphology and translocate slowly in vitro. Cells from more proximal regions tend to be bipolar and translocate more rapidly. Distal and proximal cells also probably differ in their adhesive strengths. In the mutant, talpid3, distal and proximal cells do not differ in the above properties and cells from all regions of the limb-bud are multipolar, translocate slowly and are more adhesive than normal cells. A study of light micrographs and scanning electron micrographs suggests that these regional differences are found in the limb-bud in vivo and are not merely an effect produced by the in vitro culturing system.


2019 ◽  
Vol 57 (4) ◽  
pp. 428
Author(s):  
Vu Thi Hong Quan ◽  
Nguyen Thi Quan ◽  
Mai Thanh Phong ◽  
Le Thi Kim Phung

Therefore, the physicochemical properties of Sterculia foetida L. gum, including solubility, scanning electron micrographs, melting point, swelling index, pH, viscosity, loss on drying were determined. Furthermore, compressed tablets were successfully prepared for in vitro studies at many different particle sizes, concentrations of gum, rotational speeds and media in order to evaluate the effects of these parameters on the rate of drug release. Besides, SFG tablets were compared with the tablets prepared with Hydroxymethylcellulose E15. The findings indicated that Sterculia foetida L. gum exhibited an excellent potential in managed release dosage forms. 


Zootaxa ◽  
2004 ◽  
Vol 558 (1) ◽  
pp. 1 ◽  
Author(s):  
ROBERT MESIBOV

Bromodesmus catrionae n. gen., n. sp. (type species), B. militaris n. sp., B. riparius n. sp. and B. rufus n. sp. are described. The new genus is characterized by greatly reduced paranota and a gonopod telopodite expanded at the distal end into a posteriorly concave hood fringed with teeth; the hood partly protects a long, curved, acutely pointed solenomerite. Male leg setation in the type species of six Tasmanian dalodesmid genera is briefly discussed and illustrated with scanning electron micrographs. The sphaerotrichome shaft is sharply pointed in Atrophotergum; gently tapered in Dasystigma, Lissodesmus and Tasmanodesmus; expanded at the tip in Bromodesmus; and entirely absent in Gasterogramma. Tips of the setae forming the dense ventral brush on male podomeres are gently tapered in Dasystigma and Lissodesmus, truncated in Gasterogramma, expanded in Bromodesmus and forked in Tasmanodesmus.


Parasitology ◽  
1971 ◽  
Vol 62 (3) ◽  
pp. 479-488 ◽  
Author(s):  
Gwendolen Rees

Scanning electron-micrographs have shown the covering of microvilli on the surface of the redia of Parorchis acanthus. In the contracted state the elongated microvilli with bulbous extremities seen in the surface grooves may be the result of compression. The surface of the epidermis of the cercaria is smooth on a large area of the ventral surface and lattice-like with microvilli, laterally, anteriorly, dorsally and on the tail. The spines on the body can be withdrawn into sheaths by the contraction of muscle fibres inserted into the basement lamina below each spine.I would like to express my sincere gratitude to Dr I. ap Gwynn of this department for preparing the scanning electron-micrographs and the School of Engineering Science, University of North Wales, Bangor for the use of their stereoscan. I should also like to thank Mr M. C. Bibby for technical assistance and Professor E. G. Gray and Dr W. Sinclair for assistance with the transmission electron-micrographs.


2005 ◽  
Vol 137 (5) ◽  
pp. 516-531 ◽  
Author(s):  
Nickolas G. Kavallieratos ◽  
Željko Tomanović ◽  
Christos G. Athanassiou ◽  
Petr Starý ◽  
Vladimir Žikić ◽  
...  

AbstractWe present new information on the parasitoids (Hymenoptera: Braconidae: Aphidiinae) of aphids infesting cotton, tobacco, citrus, and cereal agroecosystems in southeastern Europe. Nineteen species are keyed and illustrated with scanning electron micrographs and line drawings. The aphidiines presented in this work have been identified from 18 aphid taxa. Furthermore, 96 original parasitoid–aphid–plant associations are presented. Cotton, tobacco, and citrus agroecosystems are connected through aphids and their parasitoids, whereas cereal agroecosystems represent a separate group of associations.


Zootaxa ◽  
2018 ◽  
Vol 4378 (1) ◽  
pp. 98 ◽  
Author(s):  
MUSTAFA E. GHALIOW ◽  
ANDJELJKO PETROVIĆ ◽  
KORANA KOCIĆ ◽  
JELISAVETA ČKRKIĆ ◽  
ANA MITROVSKI BOGDANOVIĆ ◽  
...  

Although Aphidiinae parasitoids have been used as agents for biocontrol of alfalfa aphids for more than half a century and have been the subject of numerous ecological investigations, there is an evident lack of systematic studies on the parasitoids of aphids infesting alfalfa in Europe. Here we present an original and up-dated key for identification of both native and invasive Aphidiinae parasitoids attacking aphids that feed on alfalfa in Europe. In total, 20 species are keyed and illustrated with scanning electron micrographs and line drawings. 


RSC Advances ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 4109-4120 ◽  
Author(s):  
Rohit Saraf ◽  
C. Shivakumara ◽  
Sukanti Behera ◽  
H. Nagabhushana ◽  
N. Dhananjaya

Crystal structure of tetragonal BiOCl, field emission scanning electron micrographs, CIE chromaticity diagram and percentage of RhB dye degradation of Bi1−xEuxOCl.


2011 ◽  
Vol 117-119 ◽  
pp. 1129-1132
Author(s):  
Bo Liu ◽  
Hai Feng Chen ◽  
Pei Song Tang

In this experiment, pyrophyllite as the main materials used the flame photometer to measure adsorption. Using the qualitative analysis of the samples of XRD with internal structure and found that pyrophyllite used in the experiment as a natural mineral, the presence with other minerals. Meanwhile, scanning electron micrographs (SEM) from the display showed that the effect of pyrophyllite adsorption for cations should be more significant. Therefore, this experiment focused on the time, Na+ concentration, on the pyrophyllite and different pyrophyllite concentrations (adsorbent dosage) effect on the adsorption, in addition to the adsorption dynamics. The results showed that pyrophyllite adsorption sodium in the best time of equilibrium was 20 minutes, the concentration of NaCl was 50 mg/L, pH = 7, temperature was 313 K, the concentration of pyrophyllite was 5 mg/L.


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