Cultivation of Periodontal Ligament Fibroblasts on Dental Implant Materials and Enzymatically Debrided Teeth

1974 ◽  
Vol 53 (6) ◽  
pp. 1368-1376 ◽  
Author(s):  
T.K. Huard ◽  
L.F. Arnold ◽  
P. Baram
Keyword(s):  
Rhesus Monkey ◽  
Methyl Methacrylate ◽  
Dental Implant ◽  
Electron Micrographs ◽  
Periodontal Ligament ◽  
Scanning Electron Micrographs ◽  
Periodontal Ligament Fibroblasts ◽  
Implant Materials ◽  
Scanning Electron

Rhesus monkey periodontal ligament-derived fibroblasts were cultured on glass, Vitallium, poly(methyl methacrylate) and enzymatically debrided teeth. Scanning electron micrographs of these preparations and of the periodontal ligament surrounding normal and replanted teeth were compared. The fibroblasts cultured in vitro could organize on implant material and enzymatically debrided teeth to produce a network with fibers resembling those that are seen in the normal periodontal ligament.

scholarly journals Lysine bioavailability among 2 lipid-coated lysine products after exposure to silage1

2017 ◽  
Vol 1 (3) ◽  
pp. 311-319 ◽  
Author(s):  
J. N. Reiners ◽  
J. E. Held ◽  
C. L. Wright ◽  
Q. Qiao ◽  
G. D. Djira ◽  
...  
Keyword(s):  
Electron Micrographs ◽  
Corn Silage ◽  
Scanning Electron Micrographs ◽  
Visual Evaluation ◽  
Peripheral Surface ◽  
Physical And Chemical ◽  
Direct Measures ◽  
Scanning Electron

Abstract We conducted 2 experiments to determine lysine bioavailability from 2 lipid-coated lysine products. In an in vitro experiment we mixed each lipid-coated lysine product with either alfalfa- or corn-silage at different amounts of acidity. Scanning electron micrographs indicated that surface structure of each lipid-coated lysine particle was eroded after mixing with silage. Additionally, visual evaluation of scanning electron micrographs suggested that peripheral surface abrasion of lipid-coated lysine may be greater when lipid-coated lysine was mixed with alfalfa silage in comparison to corn silage. In a corresponding experiment, in vivo measures of lysine bioavailability to sheep from 2 lipid-coated lysine products and lysine-HCl were determined after mixing in corn silage. Plasma lysine concentrations increased linearly (P < 0.01) in response to abomasal lysine infusion indicating that our model was sensitive to increases in metabolizable lysine flow. Bioavailability of each lipid-coated lysine source and dietary lysine-HCl were calculated to be 23, 15, and 18%, respectively. Even though each dietary source of lysine increased plasma lysine, rates of increases in plasma lysine from one lipid-coated lysine source (linear; P = 0.20) and lysine-HCl (linear; P = 0.11) were not different from plasma lysine levels supported by diet alone. However, the rate of plasma lysine increase in response to lysine from the other lipid-coated lysine source was greater (P = 0.04) than plasma lysine from feed alone. Nonetheless, the rate of plasma lysine increase in response to lipid-coated lysine did not differ (P ≥ 0.70) from the rate of plasma lysine increase from lysine-HCl. Clearly, methods of manufacture, together with physical and chemical characteristics of diet, can impact amounts of metabolizable lysine provided from lipid-coated lysine products. Direct measures of lysine bioavailability from lipid-coated lysine products after mixing with diets should be based on measurements with the products treated similarly to the method of feeding.

scholarly journals EVALUATION OF STERCULIA FOETIDA L. GUM AS NATURAL BASED CONTROLLED RELEASE EXCIPIENT

2019 ◽  
Vol 57 (4) ◽  
pp. 428
Author(s):  
Vu Thi Hong Quan ◽  
Nguyen Thi Quan ◽  
Mai Thanh Phong ◽  
Le Thi Kim Phung
Keyword(s):  
Melting Point ◽  
Controlled Release ◽  
Drug Release ◽  
Physicochemical Properties ◽  
Electron Micrographs ◽  
Dosage Forms ◽  
Particle Sizes ◽  
Scanning Electron Micrographs ◽  
Scanning Electron

Therefore, the physicochemical properties of Sterculia foetida L. gum, including solubility, scanning electron micrographs, melting point, swelling index, pH, viscosity, loss on drying were determined. Furthermore, compressed tablets were successfully prepared for in vitro studies at many different particle sizes, concentrations of gum, rotational speeds and media in order to evaluate the effects of these parameters on the rate of drug release. Besides, SFG tablets were compared with the tablets prepared with Hydroxymethylcellulose E15. The findings indicated that Sterculia foetida L. gum exhibited an excellent potential in managed release dosage forms. 

scholarly journals In Vitro Compression Model for Orthodontic Tooth Movement Modulates Human Periodontal Ligament Fibroblast Proliferation, Apoptosis and Cell Cycle

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 932
Author(s):  
Julia Brockhaus ◽  
Rogerio B. Craveiro ◽  
Irma Azraq ◽  
Christian Niederau ◽  
Sarah K. Schröder ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Periodontal Ligament ◽  
Environmental Changes ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Compressive Force ◽  
Periodontal Ligament Fibroblasts ◽  
Human Periodontal Ligament Fibroblasts

Human Periodontal Ligament Fibroblasts (hPDLF), as part of the periodontal apparatus, modulate inflammation, regeneration and bone remodeling. Interferences are clinically manifested as attachment loss, tooth loosening and root resorption. During orthodontic tooth movement (OTM), remodeling and adaptation of the periodontium is required in order to enable tooth movement. hPDLF involvement in the early phase-OTM compression side was investigated for a 72-h period through a well-studied in vitro model. Changes in the morphology, cell proliferation and cell death were analyzed. Specific markers of the cell cycle were investigated by RT-qPCR and Western blot. The study showed that the morphology of hPDLF changes towards more unstructured, unsorted filaments under mechanical compression. The total cell numbers were significantly reduced with a higher cell death rate over the whole observation period. hPDLF started to recover to pretreatment conditions after 48 h. Furthermore, key molecules involved in the cell cycle were significantly reduced under compressive force at the gene expression and protein levels. These findings revealed important information for a better understanding of the preservation and remodeling processes within the periodontium through Periodontal Ligament Fibroblasts during orthodontic tooth movement. OTM initially decelerates the hPDLF cell cycle and proliferation. After adapting to environmental changes, human Periodontal Ligament Fibroblasts can regain homeostasis of the periodontium, affecting its reorganization.

scholarly journals Graphene-Doped Poly (Methyl-Methacrylate) (Pmma) Implants: A Micro-CT and Histomorphometrical Study in Rabbits

2021 ◽  
Vol 22 (3) ◽  
pp. 1441
Author(s):  
Antonio Scarano ◽  
Tiziana Orsini ◽  
Fabio Di Carlo ◽  
Luca Valbonetti ◽  
Felice Lorusso
Keyword(s):  
Methyl Methacrylate ◽  
Dental Implant ◽  
Animal Studies ◽  
White Male ◽  
Biological Response ◽  
Medullary Canal ◽  
Poly Methyl Methacrylate ◽  
Titanium Surfaces ◽  
Dental Applications

Background—the graphene-doping procedure represents a useful procedure to improve the mechanical, physical and biological response of several Polymethyl methacrylate (PMMA)-derived polymers and biomaterials for dental applications. The aim of this study was to evaluate osseointegration of Graphene doped Poly(methyl methacrylate) (GD-PMMA) compared with PMMA as potential materials for dental implant devices. Methods—eighteen adult New Zealand white male rabbits with a mean weight of approx. 3000 g were used in this research. A total of eighteen implants of 3.5 mm diameter and 11 mm length in GD-PMMA and eighteen implants in PMMA were used. The implants were placed into the articular femoral knee joint. The animals were sacrificed after 15, 30 and 60 days and the specimens were evaluated by µCT and histomorphometry. Results—microscopically, all 36 implants, 18 in PMMA and 18 in DG-PMMA were well-integrated into the bone. The implants were in contact with cortical bone along the upper threads, while the lower threads were in contact with either newly formed bone or with marrow spaces. The histomorphometry and µCT evaluation showed that the GP-PMMA and PMMA implants were well osseointegrated and the bone was in direct contact with large portions of the implant surfaces, including the space in the medullary canal. Conclusions—in conclusion, the results suggest that GD-PMMA titanium surfaces enhance osseointegration in rabbit femurs. This encourages further research to obtain GD-PMMA with a greater radiopacity. Also, further in vitro and vivo animal studies are necessary to evaluate a potential clinical usage for dental implant applications.

A new genus and four new species of millipedes from Tasmania, Australia (Diplopoda: Polydesmida: Dalodesmidae), with notes on male leg setae in some Tasmanian dalodesmids

Zootaxa ◽  
2004 ◽  
Vol 558 (1) ◽  
pp. 1 ◽  
Author(s):  
ROBERT MESIBOV
Keyword(s):  
New Species ◽  
Electron Micrographs ◽  
Type Species ◽  
New Genus ◽  
Scanning Electron Micrographs ◽  
Scanning Electron

Bromodesmus catrionae n. gen., n. sp. (type species), B. militaris n. sp., B. riparius n. sp. and B. rufus n. sp. are described. The new genus is characterized by greatly reduced paranota and a gonopod telopodite expanded at the distal end into a posteriorly concave hood fringed with teeth; the hood partly protects a long, curved, acutely pointed solenomerite. Male leg setation in the type species of six Tasmanian dalodesmid genera is briefly discussed and illustrated with scanning electron micrographs. The sphaerotrichome shaft is sharply pointed in Atrophotergum; gently tapered in Dasystigma, Lissodesmus and Tasmanodesmus; expanded at the tip in Bromodesmus; and entirely absent in Gasterogramma. Tips of the setae forming the dense ventral brush on male podomeres are gently tapered in Dasystigma and Lissodesmus, truncated in Gasterogramma, expanded in Bromodesmus and forked in Tasmanodesmus.

The ultrastructure of the epidermis of the redia and cercaria of Parorchis acanthus, Nicoll. A study by scanning and transmission electron-microscopy

Parasitology ◽  
1971 ◽  
Vol 62 (3) ◽  
pp. 479-488 ◽  
Author(s):  
Gwendolen Rees
Keyword(s):  
Electron Micrographs ◽  
Technical Assistance ◽  
Ventral Surface ◽  
The Body ◽  
Muscle Fibres ◽  
Scanning Electron Micrographs ◽  
Large Area ◽  
Transmission Electron ◽  
Parorchis Acanthus ◽  
Scanning Electron

Scanning electron-micrographs have shown the covering of microvilli on the surface of the redia of Parorchis acanthus. In the contracted state the elongated microvilli with bulbous extremities seen in the surface grooves may be the result of compression. The surface of the epidermis of the cercaria is smooth on a large area of the ventral surface and lattice-like with microvilli, laterally, anteriorly, dorsally and on the tail. The spines on the body can be withdrawn into sheaths by the contraction of muscle fibres inserted into the basement lamina below each spine.I would like to express my sincere gratitude to Dr I. ap Gwynn of this department for preparing the scanning electron-micrographs and the School of Engineering Science, University of North Wales, Bangor for the use of their stereoscan. I should also like to thank Mr M. C. Bibby for technical assistance and Professor E. G. Gray and Dr W. Sinclair for assistance with the transmission electron-micrographs.

Aphid parasitoids infesting cotton, citrus, tobacco, and cereal crops in southeastern Europe: aphid-plant associations and keys

2005 ◽  
Vol 137 (5) ◽  
pp. 516-531 ◽  
Author(s):  
Nickolas G. Kavallieratos ◽  
Željko Tomanović ◽  
Christos G. Athanassiou ◽  
Petr Starý ◽  
Vladimir Žikić ◽  
...  
Keyword(s):  
Electron Micrographs ◽  
Separate Group ◽  
Cereal Crops ◽  
Scanning Electron Micrographs ◽  
Southeastern Europe ◽  
Line Drawings ◽  
Plant Associations ◽  
New Information ◽  
Aphid Parasitoids ◽  
Scanning Electron

AbstractWe present new information on the parasitoids (Hymenoptera: Braconidae: Aphidiinae) of aphids infesting cotton, tobacco, citrus, and cereal agroecosystems in southeastern Europe. Nineteen species are keyed and illustrated with scanning electron micrographs and line drawings. The aphidiines presented in this work have been identified from 18 aphid taxa. Furthermore, 96 original parasitoid–aphid–plant associations are presented. Cotton, tobacco, and citrus agroecosystems are connected through aphids and their parasitoids, whereas cereal agroecosystems represent a separate group of associations.

Key for identification of the parasitoids (Hymenoptera: Braconidae: Aphidiinae) of aphids infesting alfalfa in Europe

Zootaxa ◽  
2018 ◽  
Vol 4378 (1) ◽  
pp. 98 ◽  
Author(s):  
MUSTAFA E. GHALIOW ◽  
ANDJELJKO PETROVIĆ ◽  
KORANA KOCIĆ ◽  
JELISAVETA ČKRKIĆ ◽  
ANA MITROVSKI BOGDANOVIĆ ◽  
...  
Keyword(s):  
Electron Micrographs ◽  
Scanning Electron Micrographs ◽  
Line Drawings ◽  
The Subject ◽  
Scanning Electron

Although Aphidiinae parasitoids have been used as agents for biocontrol of alfalfa aphids for more than half a century and have been the subject of numerous ecological investigations, there is an evident lack of systematic studies on the parasitoids of aphids infesting alfalfa in Europe. Here we present an original and up-dated key for identification of both native and invasive Aphidiinae parasitoids attacking aphids that feed on alfalfa in Europe. In total, 20 species are keyed and illustrated with scanning electron micrographs and line drawings. 

Photoluminescence, photocatalysis and Judd–Ofelt analysis of Eu3+-activated layered BiOCl phosphors

RSC Advances ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 4109-4120 ◽  
Author(s):  
Rohit Saraf ◽  
C. Shivakumara ◽  
Sukanti Behera ◽  
H. Nagabhushana ◽  
N. Dhananjaya
Keyword(s):  
Crystal Structure ◽  
Field Emission ◽  
Electron Micrographs ◽  
Dye Degradation ◽  
Scanning Electron Micrographs ◽  
Chromaticity Diagram ◽  
Cie Chromaticity ◽  
Scanning Electron

Crystal structure of tetragonal BiOCl, field emission scanning electron micrographs, CIE chromaticity diagram and percentage of RhB dye degradation of Bi1−xEuxOCl.

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