scholarly journals Biofunctionalisation of Gallium Arsenide with Neutravidin

2021 ◽  
Author(s):  
Barbara Santos Gomes ◽  
David Morgan ◽  
Wolfgang Langbein ◽  
Paola Borri ◽  
Francesco Masia
Keyword(s):  
Spectroscopic Ellipsometry ◽  
Photoelectron Spectroscopy ◽  
Specific Binding ◽  
Gaas Surface ◽  
Biomolecular Recognition ◽  
Surface Characterisation ◽  
X Ray ◽  
High Affinity ◽  
Surface Functionalisation ◽  
New Generation

<div>We report a study presenting a physicochemical surface characterisation of the GaAs surface along the functionalisation with a high-affinity bioconjugation pair widely explored in the life</div><div>sciences: biotin and neutravidin. Combined X-ray photoelectron spectroscopy (XPS), wettability measurements and spectroscopic ellipsometry were used for a reliable characterisation of the surface functionalisation process. The results suggest that a film with a thickness lower than 10nm was formed, with a neutravidin to biotin ratio of 1:25 on the GaAs surface. Reduction of non-specific binding of the protein to the surface was achieved by optimising the protein buffer and rinsing steps. This study shows the feasibility of using GaAs as a platform for specific biomolecular recognition, paving the way to a new generation of optoelectronic biosensors.</div>

scholarly journals Biofunctionalisation of Gallium Arsenide with Neutravidin

2021 ◽  
Author(s):  
Barbara Santos Gomes ◽  
David Morgan ◽  
Wolfgang Langbein ◽  
Paola Borri ◽  
Francesco Masia
Keyword(s):  
Spectroscopic Ellipsometry ◽  
Photoelectron Spectroscopy ◽  
Specific Binding ◽  
Gaas Surface ◽  
Biomolecular Recognition ◽  
Surface Characterisation ◽  
X Ray ◽  
High Affinity ◽  
Surface Functionalisation ◽  
New Generation

<div>We report a study presenting a physicochemical surface characterisation of the GaAs surface along the functionalisation with a high-affinity bioconjugation pair widely explored in the life</div><div>sciences: biotin and neutravidin. Combined X-ray photoelectron spectroscopy (XPS), wettability measurements and spectroscopic ellipsometry were used for a reliable characterisation of the surface functionalisation process. The results suggest that a film with a thickness lower than 10nm was formed, with a neutravidin to biotin ratio of 1:25 on the GaAs surface. Reduction of non-specific binding of the protein to the surface was achieved by optimising the protein buffer and rinsing steps. This study shows the feasibility of using GaAs as a platform for specific biomolecular recognition, paving the way to a new generation of optoelectronic biosensors.</div>

scholarly journals Oxidation of aluminum thin films protected by ultrathin MgF2 layers measured using spectroscopic ellipsometry and X-ray photoelectron spectroscopy

OSA Continuum ◽  
2021 ◽  
Vol 4 (3) ◽  
pp. 879
Author(s):  
Brian I. Johnson ◽  
Tahereh G. Avval ◽  
R. Steven Turley ◽  
Matthew R. Linford ◽  
David D. Allred
Keyword(s):  
Thin Films ◽  
Spectroscopic Ellipsometry ◽  
Photoelectron Spectroscopy ◽  
X Ray

scholarly journals Synthesis of Heart/Dumbbell-Like CuO Functional Nanostructures for the Development of Uric Acid Biosensor

Materials ◽  
2018 ◽  
Vol 11 (8) ◽  
pp. 1378 ◽  
Author(s):  
Zafar Ibupoto ◽  
Aneela Tahira ◽  
Hamid Raza ◽  
Gulzar Ali ◽  
Aftab Khand ◽  
...  
Keyword(s):  
Uric Acid ◽  
Photoelectron Spectroscopy ◽  
Lithium Ion ◽  
Nanostructured Material ◽  
Soft Template ◽  
X Ray ◽  
Growth Method ◽  
Enzyme Molecules ◽  
New Generation ◽  
Uric Acid Biosensor

It is always demanded to prepare a nanostructured material with prominent functional properties for the development of a new generation of devices. This study is focused on the synthesis of heart/dumbbell-like CuO nanostructures using a low-temperature aqueous chemical growth method with vitamin B12 as a soft template and growth directing agent. CuO nanostructures are characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) techniques. CuO nanostructures are heart/dumbbell like in shape, exhibit high crystalline quality as demonstrated by XRD, and have no impurity as confirmed by XPS. Apparently, CuO material seems to be porous in structure, which can easily carry large amount of enzyme molecules, thus enhanced performance is shown for the determination of uric acid. The working linear range of the biosensor is 0.001 mM to 10 mM with a detection limit of 0.0005 mM and a sensitivity of 61.88 mV/decade. The presented uric acid biosensor is highly stable, repeatable, and reproducible. The analytical practicality of the proposed uric acid biosensor is also monitored. The fabrication methodology is inexpensive, simple, and scalable, which ensures the capitalization of the developed uric acid biosensor for commercialization. Also, CuO material can be used for various applications such as solar cells, lithium ion batteries, and supercapacitors.

Effect of Cleaning Chemistries on Cobalt: Surface Chemistries and Electrical Characterization

2018 ◽  
Vol 282 ◽  
pp. 263-267
Author(s):  
Quoc Toan Le ◽  
Els Kesters ◽  
Yuya Akanishi ◽  
Marleen H. van der Veen ◽  
Atsushi Mizutani ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Spectroscopic Ellipsometry ◽  
Photoelectron Spectroscopy ◽  
Good Yield ◽  
Electrical Characterization ◽  
Cobalt Hydroxide ◽  
Dissolved Oxygen Concentration ◽  
X Ray ◽  
Xps Characterization ◽  
Surface Chemistries

The etching characteristics of ECD cobalt in different cleaning solutions were characterized using four-point probe, spectroscopic ellipsometry, and X-ray photoelectron spectroscopy. 0.05% HF solution with saturated dissolved oxygen concentration was found to result in a substantial etch of ECD cobalt (~5 nm/min). In contrast, cleaning in the SC1 1:4:100 mixture and the formulated mixture led to a significantly lower etch amount, which could be explained by the formation of a passivation layer at the surface. XPS characterization indicated the formation of a cobalt hydroxide at the surface. The electrical evaluation of the DD structure carried out after cleaning using the formulated chemical mixture and subsequent metallization showed good yield for the 22 nm Kelvin vias, testifying an efficient cleaning of the Co surface at the via bottom.

scholarly journals The Surface Characterisation of Fused Filament Fabricated (FFF) 3D Printed PEEK/Hydroxyapatite Composites

Polymers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 3117
Author(s):  
Krzysztof Rodzeń ◽  
Mary Josephine McIvor ◽  
Preetam K. Sharma ◽  
Jonathan G. Acheson ◽  
Alistair McIlhagger ◽  
...  
Keyword(s):  
3D Printing ◽  
Photoelectron Spectroscopy ◽  
High Performance ◽  
Secondary Ion Mass Spectrometry ◽  
Fused Filament Fabrication ◽  
Surface Characterisation ◽  
X Ray ◽  
3D Printed

Polyetheretherketone (PEEK) is a high-performance thermoplastic polymer which has found increasing application in orthopaedics and has shown a lot of promise for ‘made-to-measure’ implants via additive manufacturing approaches. However, PEEK is bioinert and needs to undergo surface modification to make it at least osteoconductive to ensure a more rapid, improved, and stable fixation that will last longer in vivo. One approach to solving this issue is to modify PEEK with bioactive agents such as hydroxyapatite (HA). The work reported in this study demonstrates the direct 3D printing of PEEK/HA composites of up to 30 weight percent (wt%) HA using a Fused Filament Fabrication (FFF) approach. The surface characteristics and in vitro properties of the composite materials were investigated. X-ray diffraction revealed the samples to be semi-crystalline in nature, with X-ray Photoelectron Spectroscopy and Time-of-Flight Secondary Ion Mass Spectrometry revealing HA materials were available in the uppermost surface of all the 3D printed samples. In vitro testing of the samples at 7 days demonstrated that the PEEK/HA composite surfaces supported the adherence and growth of viable U-2 OS osteoblast like cells. These results demonstrate that FFF can deliver bioactive HA on the surface of PEEK bio-composites in a one-step 3D printing process.

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