Gas chromatography-mass spectrometry analysis of phytocomponents of sargassum polycystum

Sargassum polycystum is a brown seaweed, which has been reported to contain various phytoconstituents especially antimicrobial properties. However, the study on phytochemical components and biological activities of S. polycystum are yet fully understood. Therefore, the objectives of this study are to evaluate the best extraction solvents for S. polycystum and to determine the percentage of phytocomponents obtained in the n-hexane, dichloromethane (DCM) and methanol extracts of S. polycystum via Gas Chromatography-Mass Spectrometry (GC-MS) analysis. S. polycystum was collected from the coastal area of Sabah, Malaysia. After collection, it was rinsed, dried and extracted with n-hexane, DCM and methanol by soxhlet extraction. The fatty acid compounds (FAME) analysis was done using The Perkin Elmer Turbo Mass Spectrometer. This study showed that methanol is the most efficient solvent as it produced the highest extraction yield with 3.83%, followed by n-hexane with 1.69% and lastly DCM with 0.59% in S. polycystum. On top of that, this study has found 19 phytocomponents in all extraction methods of S. polycystum, which have been proven to possess antibacterial constituents such as palmitic acid, myristic acid, oleic acid, pentadecanoic acid and behenic acid as assessed by GC-MS analysis.

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Identification of Bioactive Phytocomponents of Hydroalcoholic Extract of Enhalus acoroides by Gas Chromatography- Mass Spectrometry Analysis

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.01126 ◽

2021 ◽

pp. 6511-6515

Author(s):

S. Navaith Ahmed ◽

P. Kalaivani ◽

P. Amudha ◽

B. Usharani

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Biological Activities ◽

Marine Species ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Hydroalcoholic Extract ◽

Spectrometry Analysis ◽

Enhalus Acoroides ◽

Chromatography Mass Spectrometry

Sea grass are good source of potent drug which has medicinal properties and able to cure human disease. One such marine plant is the Enhalus acoroides which belong to hydrocharitaeceae family. This marine species are widely distributed in the tropics of Indian and western Pacific Ocean and the species were collected from Ramanathapuram district for further process. This study reveals about the bioactive components present in Enhalus acoroides and identified its biological activity by Gas chromatography Mass spectrometry analysis using hydroalcoholic extract. The compounds present in Enhalus acoroides are Benzoic acid, 2- methyl 7- oxa bicyclol heptanes, 1, 3 Nonadiene, silane, ethoxytriethyl. Biological activities of the compounds present in the sample include antioxidant, hypocholesterolemic, antihypertensive, anti-inflammatory, anti-microbial, antiviral and anti-hepatotoxic effect.

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GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS OF METHANOL EXTRACT OF ZANTHOXYLUM RHETSA DC. FRUITS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.26821 ◽

2018 ◽

Vol 11 (12) ◽

pp. 247

Author(s):

Kuladip Gurav ◽

Varsha Jadhav (rathod)

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Bioactive Compounds ◽

Biological Activities ◽

Acid Methyl Ester ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Hexadecanoic Acid ◽

Spectrometry Analysis ◽

Chromatography Mass Spectrometry

Objective: The aim was to investigate important bioactive compounds, biological activities, and medicinal importance of Zanthoxylum rhetsa fruits.Methods: The present work was carried out by gas chromatography–mass spectrometry (GC-MS) method for the identification of different compounds.Result: The methanolic extract of fruits showed 32 chemical compounds which are identified through GC-MS analysis. Among them, some of the compound names and percentage values are as follows: 2-propanone, 1,3-dihydroxy (48.9%), 4H-pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (33.7%), 2-furancarboxaldehyde, 5-[hydroxymethyl] (50.2%), 1-Heptatriacontanol (34.4%), 9,12-octadecadienoic acid (zz)- (48.6%), cholestan- 3-ol,2-methylene, [3β,5α] (75.0%), 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (90.8), 2-furancarboxaldehyde, 5-[hydroxymethyl] (92.0%), hydroquinone (64.9%), n-hexadecanoic acid (37.5%), octadecanoic acid (28.2%), 9,12,15, octadecatrienoic acid, 2-[(trimethylsiyl]oxy]-1- [(trimethylsiyl]oxy]methyl] ethyl ester, [zzz] (22.6%), 9-hexadecanoic acid (10.3%), digitoxin (18.8%), 8,11,14-eicosatrienoic acid methyl ester, [zzz] (25.5%), and oleic acid (16.5%). Most of the identified compounds in the crude methanolic extracts exhibit some bioactivities, namely anticancer, anti-inflammatory, antimicrobial, hepatoprotective, antioxidant, hypocholesterolemic, nematicide, pesticide, anti-androgenic flavor, hemolytic, 5-alpha-reductase inhibitor, insectifuge, antiarthritic, anti-coronary, cardiovascular, anti-breast cancer, aromatic, and insectifuge. On the basis of the above investigation, the fruits can be recommended as a treasure of bioactive compounds and it plays a promising role in herbal medicine.Conclusion: The present study reveals that fruits of Z. rhetsa contain various bioactive compounds. Digitoxin is recorded in the ripened fruit of Z. rhetsa and it shows the anticancerous and cardiac arrest properties. Hence, in future, this plant will play a promising role in curing cancer.

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GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS AND IN VITRO ANTIMICROBIAL SCREENING OF WEDELIA GLAUCA (ORTEGA) O. HOFFM. EX HICKEN

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i11.20557 ◽

2017 ◽

Vol 10 (11) ◽

pp. 109

Author(s):

Krishnavignesh L Krishnavignesh ◽

Mahalakshmipriya A ◽

Ramesh M

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Antimicrobial Activity ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Spectrometry Analysis ◽

Chromatography Mass Spectrometry ◽

Ms Analysis

Objective: Continued resistance toward the antibiotics urges us to explore newer antibiotics. Plants are being the safer source of antibiotics with lesser or no side effects. This study was designed to study the presence of phytochemical constituents and antibacterial activity of leaf and flower extracts of Wedelia glauca against urinary tract infection causing pathogens.Methods: The plant leaves were extracted with five different solvents based on the polarity. The extraction was done using soxhalation. Antimicrobial activity was determined by agar well diffusion method for both the sample and standard. The acetone plant extract was subjected to gas chromatography-mass spectrometry (GC-MS) analysis for screening phytoconstituents.Results: Preliminary phytochemical screening revealed the presence of diverse phytoconstituents in the plant. The different extracts exhibited a considerable antimicrobial potential. Among the solvents used acetone extract showed comparably better antimicrobial activity with 100% of inhibition rate with the maximum zone of inhibition of 1.6±0.77 mm against Staphylococcus sp. and Aspergillus sp. at the concentration of 5 mg. GC-MS analysis provided 8 major peaks which revealed the existence of a variety of bioactive compounds which may attribute to the efficacy of the plant.Conclusion: W. glauca leaf and flower extracts displayed a broad spectrum of antibacterial and antifungal activity and can be considered as a potential source of newer antibiotic compounds.

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PHYTOCHEMICAL STUDIES AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF SARCOSTEMMA BREVISTIGMA, WIGHT & ARN.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i3.16538 ◽

2017 ◽

Vol 10 (3) ◽

pp. 462 ◽

Cited By ~ 1

Author(s):

Dhivya S M ◽

Kalaichelvi K

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Ethanolic Extract ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Analysis ◽

Spectrometry Analysis ◽

Chromatography Mass Spectrometry ◽

Ms Analysis ◽

Sarcostemma Brevistigma

ABSTRACTObjective: To explore the phytochemical constituents present in Sarcostemma brevistigma using gas chromatography-mass spectrometry (GC-MS).Methods: Preliminary phytochemical analysis of secondary metabolites was made by following standard procedures. GC-MS analysis of the plantextracts was performed by using GC-MS equipped with a DB-35MS capillary standard non-polar column and gas chromatograph interfaced to a MassSelective Detector (MS-DSQ-II) with Xcalibur software.Results: The qualitative phytochemical analysis of this species exhibited the presence of alkaloids, glycosides, flavonoids, phenols, steroids, aminoacids, tannins, terpenoids, quinones, and coumarin. The GC-MS analysis revealed the presence of 24 compounds in the ethanolic extract of aerial partof Sarcostemma brevistigma. The most prevailing compound was 14,17 bis(dimethylaminoamino)[3.3]paracyclophan-5,8-diacetonitrile (14.86%).Conclusion: The study concludes that the species Sarcostemma brevistigma is a potential source for bioactive compounds such as esters, alkanes,alcohols, alkenes, amide, amine, phenol, ketone, and so forth. This study justifies the traditional usage of this species.Keywords: Gas chromatography-mass spectrometry, Sarcostemma brevistigma, Phytochemical compounds, Medicinal plants.

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