Invitro and insilico anti hyperglycemic activity of cadaba fruticosa leaves - an enteric approach

In the present study, an effort was made to identify the anti hyperglycemic potential of ethanolic extract of Cadaba fruticosa leaves concentrating the enteric system. The glucose-lowering potential was studied by using the in vitro methods such as α amylase inhibitory activity, inhibition of glucose diffusion, glucose adsorption and uptake by yeast cells. In silico methods comprising of the molecular docking of the selected phyto constituents as antagonistic ligands to the disaccharide digesting enzyme α glucosidase and glucose level maintaining enzyme dipeptidyl peptidase IV. The plant extract inhibited α amylase enzyme and the glucose diffusion considerably, which was found to be concentration-dependent. The uptake of glucose by the yeast cells in the presence of the extract was also found to be increased. In the molecular docking analysis based on the docking score, iso quercetin was found to be a potential antagonistic ligand for both enzymes α glucosidase and dipeptidyl peptidase IV since they exhibited similar amino acid interactions shown by the acarbose and Sitagliptin which are the standard competitive inhibitors of these two enzymes. It can be concluded that the leaves of Cadaba fruticosa may serve as a potential anti hyperglycemic drug in future for the management of diabetes mellitus.