scholarly journals Significance of Hyaluronic acid as a biomarker with seropositive and seronegative autoantibodies in Rheumatoid arthritis patients

2021 ◽  
Vol 12 (2) ◽  
pp. 1322-1329
Author(s):  
Vinod A N ◽  
Preeti R Y ◽  
Riya K ◽  
Ruthvik N ◽  
Prahaladh R
Keyword(s):  
Rheumatoid Arthritis ◽  
Hyaluronic Acid ◽  
Statistical Significance ◽  
Joint Destruction ◽  
Autoimmune Disorder ◽  
Cross Sectional Study ◽  
Synovial Inflammation ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Female Population

Testing for autoantibodies is a flagship feature of Rheumatoid arthritis (RA), a chronic inflammatory autoimmune disorder affecting both the male and female population. Synovial inflammation followed by cartilage, bone, and joint destruction in the later stages of RA puts life in peril, especially for those with other comorbidities. In this study, we focused on to measure serum Hyaluronic acid (HA) along with seropositive and seronegative RF, AntiCCP autoantibodies to establish any association with these biomarkers. It was a cross-sectional study involving 152 RA patients based on the 1987 ACR criteria for the diagnosis of RA and 68 age‑ and sex-matched healthy controls. After clinical examination, the traditional markers were assessed to measure the disease activity, such as CRP, ESR, Anti -CCP, and RF in RA patients. The serum HA levels were measured using the ELISA method. All the values were expressed as median (25th–75th percentile). Based on seropositive and seronegative RF and AntiCCP autoantibodies, the patient group was divided into four groups- both seropositive, both seronegative, and the other two mixed groups. The traditional inflammatory markers were significantly increased in RA patients than in controls with (p  < 0.001). In our study, there was a significant increase in serum HA levels in RA patients compared to healthy controls (p  < 0.03). The serum HA levels were significantly correlated with Anti-CCP, DAS‑28, CRP, and ESR in RA patients. At the same time, serum HA level is increased in the group with seropositive for both antibodies showing statistical significance. Conclusion- serum Hyaluronic acid is involved in synovial inflammation, manifesting a common triggering mechanism more with AntiCCP antibodies than RF, promising for better clinical utility in the early stages of rheumatoid arthritis.

scholarly journals Interleukin-37 as an anti-inflammatory cytokine: does its relation to disease activity suggest its potential role in rheumatoid arthritis therapy?

2021 ◽  
Vol 48 (1) ◽  
Author(s):  
Eman A. Baraka ◽  
Mona G. Balata ◽  
Shereen H. Ahmed ◽  
Afaf F. Khamis ◽  
Enas A. Elattar
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Cross Sectional Study ◽  
Healthy Controls ◽  
Tender Joint ◽  
Cross Sectional ◽  
Reactive Protein ◽  
Significant Difference ◽  
The Mean ◽  
Anti Inflammatory

Abstract Background This study aimed to measure the serum and synovial interleukin (IL)-37 levels in rheumatoid arthritis (RA) patients compared to patients with primary knee osteoarthritis (PKOA) and healthy controls and to detect its relation to RA disease activity. Results This cross-sectional study included 50 RA patients with a mean age of 40.24 ± 8.62 years, 50 patients with PKOA with a mean age of 56.69 ± 4.21, and 40 healthy controls with a mean age of 41.75 ± 7.38 years. The mean serum IL-37 level in the RA patients (382.6 ± 73.97 pg/ml) was statistically significantly (P < 0.001) the highest among the studied groups; however, it showed a non-significant difference between the PKOA patients (70.38 ± 27.49 pg/ml) and the healthy controls (69.97 ± 25.12 pg/ml) (P > 0.94). Both serum and synovial IL-37 levels were significantly positively correlated with disease activity scores (r = 0.92, P< 0.001 and r = 0.85, P < 0.001), tender joint counts (r = 0.83, P < 0.001 and r = 0.82, P < 0.001 ), swollen joint counts (r = 0.72, P < 0.001 and r = 0.60, P < 0.001), visual analog scale (r = 0.82, P < 0.001 and r = 0.82, P < 0.001), erythrocyte sedimentation rate (r = 0.75, P < 0.001 and r = 0.65, P < 0.001), and C-reactive protein (r = 0.93, P < 0.001 and r = 0.79, P < 0.001), respectively. Conclusion Serum and synovial IL-37 were significantly elevated in the RA patients, and they were closely correlated. Being less invasive, the serum IL-37 could be a marker of disease activity and could reflect the effective disease control by drugs. Having an anti-inflammatory effect could not suggest IL-37 as the key player to control inflammation alone, but its combination with other anti-proinflammatory cytokines could be investigated.

scholarly journals Factors affecting carotid intimal medial thickness in patients with rheumatoid arthritis, an analytical cross-sectional study

2019 ◽  
Vol 6 (3) ◽  
pp. 628
Author(s):  
K. M. Prabhuswamy ◽  
M. Virgin Joena
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Intima Media Thickness ◽  
Carotid Intima Media Thickness ◽  
Cross Sectional Study ◽  
Healthy Controls ◽  
Sectional Study ◽  
Cross Sectional ◽  
Media Thickness ◽  
The Mean

Background: IMT assessment as a non-invasive imaging test is quite widely used especially among RA patients, the clinical applications of using such knowledge is scarce, hence study was conducted to compare the carotid artery intima-media thickness (CIMT) in patients with rheumatoid arthritis (RA) with healthy controls also to study the correlation between duration of rheumatoid arthritis, the activity of rheumatoid arthritis and other factors influencing (CIMT).Methods: In analytical cross-sectional study, of 80 participants of RA and 40 healthy controls, “DAS28” was used to assess disease activity.  Carotid intima-media thickness assessed using carotid ultrasonography.Results: Mean age of the cases and controls was 43.9 and 44.38 years. Subjects with duration of disease <2 years, to 5 years and >5 years were 35%, 45% and 20%. The mean carotid intima-media thickness was 5.61mm in controls, and CIMT was 6.11mm in people below 2 years and 7.08 mm in people between 2 to 5 years and 8.00mm in people above 5 years which was statistically significant. The mean carotid intima-media thickness was 5.61mm controls and 6.86mm in people with low, 7.00mm in people with moderate and 6.95mm in people with high disease activity, which was statistically significant.Conclusions: Study findings revealed risk of increase in carotid intima-media thickness higher among RA patients in the later stages and can increase the patients’ susceptibility to cardiovascular events. The factors showing strong association with intimal medial thickness were the age and symptoms duration.

scholarly journals Demographic, Clinical and Immunogenetic Profiles of a Greek Cohort of COVID-19 Patients

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1017
Author(s):  
Maria. G. Detsika ◽  
Chara Giatra ◽  
Vasiliki Kitsiou ◽  
Edison Jahaj ◽  
Theofilos Athanassiades ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Cross Sectional Study ◽  
Hospitalized Patients ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Positive Allele ◽  
Disease Characteristics ◽  
Present Cross Sectional Study ◽  
Specific Allele ◽  
Males And Females

The present cross-sectional study consists of a comprehensive analysis of epidemiological, laboratory, and clinical characteristics of COVID-19 patients in relation to their immunogenetic profiles. We studied 125 COVID-19 patients comprising different stages of disease severity; non-hospitalized (mild n = 69) and hospitalized (n = 56). Analysis of disease characteristics revealed no major differences between males and females of each group of patients while hospitalized patients were older and presented with comorbidities. A positive allele association was observed for HLA-DRB1*01 in total COVID-19 patients versus healthy controls. Subgrouping of COVID-19 patients in mild and hospitalized further identified a statistically significant increase in HLA-DRB1*01 in mild COVID-19 patients versus controls. The frequency of A*11, A*23, and DRB1*09 alleles was higher, while the frequency of C*12 was lower, in hospitalized patients versus healthy controls albeit with uncorrected statistical significance. The identification of specific allele associations may provide useful future markers for disease susceptibility in order to allow successful clinical management of COVID-19 patients.

scholarly journals Study of serum traditional and nontraditional biomarkers in Rheumatoid arthritis patients

2020 ◽  
Vol 11 (4) ◽  
pp. 7585-7592
Author(s):  
Vinod A N ◽  
Leena Chand ◽  
Preeti R Y ◽  
Harshitha S ◽  
Prahaladh R
Keyword(s):  
Rheumatoid Arthritis ◽  
Inflammatory Markers ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Cross Sectional Study ◽  
Healthy Controls ◽  
Sectional Study ◽  
Cross Sectional ◽  
Significant Difference

The study of biomarkers in Rheumatoid arthritis (RA) is highly indispensable to understand mechanisms of pathogenesis, diagnosis, prognosis, and treatment of disease. The role of traditional biomarkers like Anti-CCP, RF, and inflammatory markers like ESR and CRP is well established. In this study, we aimed to measure nontraditional biomarkers like Hyaluronic acid (HA), Cartilage oligomeric matrix protein (COMP), and Osteocalcin in the serum of RA patients and also to establish an association with traditional markers. It was a cross-sectional study involving 152 RA patients based on the 1987 ACR criteria for the diagnosis of RA and 68 age‑ and sex-matched healthy controls. After the clinical examination, traditional markers were assessed to measure the disease activity along with non traditional markers in RA patients. All the values were expressed as median (25th–75th percentile). In our study, there was a significant increase in serum HA levels in RA patients compared to healthy controls (p  < 0.03), whereas no significant difference in serum COMP and osteocalcin levels. The traditional inflammatory markers were significantly increased in RA patients than controls with (p  < 0.001). The serum HA levels were significantly correlated with traditional markers in RA patients. Conclusion: Significant increase in serum HA level in RA patients indicating synovial inflammation, but there were no notable changes in COMP and osteocalcin level in serum presuming the combination of these markers may be useful along with traditional markers in the different stages of RA.

Rheumatoid Arthritis: Seropositivity versus Seronegativity; A Comparative Cross-sectional Study Arising from Moroccan Context

2020 ◽  
Vol 16 (2) ◽  
pp. 143-148
Author(s):  
Ksir Salma ◽  
Akasbi Nessrine ◽  
Efemba Krystel ◽  
El Kinany Khaoula ◽  
Naji Noura ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Joint Destruction ◽  
Bone Destruction ◽  
Cross Sectional Study ◽  
Clinical Severity ◽  
Bivariate Analysis ◽  
Sectional Study ◽  
Cross Sectional ◽  
Systemic Manifestations ◽  
Citrullinated Protein

Introduction: Rheumatoid arthritis (RA) is biologically marked by a positive serum rate of rheumatoid factor (RA) and/or anti-citrullinated protein antibodies (ACPA). Nevertheless, 20% of RA cases remain seronegative. Objective: The main purpose of this study, is to bring out the clinical, biological, imaging, therapeutic, and evolutionary distinctions between seropositive RA and seronegative one. Methods: This is an observational cross-sectional study that involves patients with RA admitted in the rheumatology department, from the period between January 2012 and January 2018. RA seronegativity is described as the absence of both RF and ACPA, while seropositivity is recognized by the presence of at least one of the two antibodies. Results: 294 patients were included, of which 90% were seropositive and 10% seronegative. Therefore, RA in this study is seropositive most often. The bivariate analysis underscored plenty of differences, statistically notable, according to the RA immune status. In fact, patients with seropositive RA had more synovitis (p=0.049), more deformities (p=0.01), and more bone destruction on radiographs (p=0.04). Furthermore, RA in this Moroccan study was quite severe (p=0.006) and got more complicated by systemic manifestations (p=0.02). Whereas, no distinction was brought up between the two groups, concerning the use of biotherapy. As for the multivariate study, seropositive RA in these patients, had greater severity (p=0.009, OR=4.53) and was more deforming (p=0.03 OR=2.45). Conclusion: RA in our Moroccan context is dominated by the seropositive form. This seropositivity is often coupled with clinical severity and joint destruction, resulting in more deformities.

scholarly journals Osteoprotegerin Polymorphisms in a Mexican Population with Rheumatoid Arthritis and Generalized Osteoporosis: A Preliminary Report

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Maria Guadalupe Zavala-Cerna ◽  
Maria Cristina Moran-Moguel ◽  
Jesus Alejandro Cornejo-Toledo ◽  
Norma Guadalupe Gonzalez-Montoya ◽  
Jose Sanchez-Corona ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Duration ◽  
Statistical Significance ◽  
Osteoclast Differentiation ◽  
Cross Sectional Study ◽  
Nuclear Factor ◽  
Mineral Density ◽  
Cross Sectional ◽  
Allele Distribution ◽  
Receptor Activator

Bone disease in rheumatoid arthritis (RA) is a complex phenomenon where genetic risk factors have been partially evaluated. The system formed by receptor activator for nuclear factor-κB (RANK), receptor activator for nuclear factor-κB ligand (RANKL), and osteoprotegerin (OPG): RANK/RANKL/OPG is a crucial molecular pathway for coupling between osteoblasts and osteoclasts, since OPG is able to inhibit osteoclast differentiation and activation. We aim to evaluate the association between SNPs C950T (rs2073617), C209T (rs3134069), T245G (rs3134070) in theTNFRSF11B(OPG) gene, and osteoporosis in RA. We included 81 women with RA and 52 healthy subjects in a cross-sectional study, genotyped them, and measured bone mineral density (BMD) at the lumbar spine and the femoral neck. Mean age in RA was50±12with disease duration of12±8years. According to BMD results, 23 (33.3%) were normal and 46 (66.7%) had osteopenia/osteoporosis. We found a higher prevalence of C allele for C950T SNP in RA. Polymorphisms C209T and T245G did not reach statistical significance in allele distribution. Further studies including patients from other regions of Latin America with a multicenter design to increase the sample size are required to confirm our findings and elucidate if C950T SNP could be associated with osteoporosis in RA.

The outcome of patients with rheumatoid arthritis: a cross-sectional study

2019 ◽  
pp. 5-9
Author(s):  
Khaled Algohani ◽  
Muhannad Althobaiti ◽  
Sanad Alshammari ◽  
Fahad Alnahari ◽  
Ali Aldahhasi ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional
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