scholarly journals STABILIZING ABILITY OF HUMIC SUBSTANCES AND MICRO-ORGANISMS OF THE GENUS RHODOCOCCUS IN THE RELATION TO OIL PRODUCTS

2020 ◽  
pp. 112-123
Author(s):  
Мария Михайловна Герцен ◽  
Елена Дмитриевна Дмитриева
Keyword(s):  
Humic Substances ◽  
Rhodococcus Erythropolis ◽  
Oil Products ◽  
Oil Hydrocarbons ◽  
High Moor ◽  
Micro Organisms ◽  
High Moor Peat

Изучено влияние микроорганизмов рода Rhodococcus на стабилизирующую способность гуминовых веществ торфов по отношению к углеводородам нефти. Установлено, что наиболее стабильными являются водные эмульсии гексадекана и нефти в присутствии микроорганизмов Rhodococcus erythropolis S67 и гуминовые вещества тростникового низинного торфа, а по отношению к водной эмульсии дизельного топлива - гуминовые вещества сфагнового верхового торфа и Rhodococcus erythropolis Х5. Доказано, что полученная биокомпозиция на основе гуминовых веществ торфов и микроорганизмов рода Rhodococcus способна стабилизировать эмульсии углеводородов нефти в воде за счет связывания их гуминовыми веществами торфов в нетоксичные комплексы. The effect of microorganisms of the genus Rhodococcus on the stabilization ability of humic substances of peats in the relation to oil hydrocarbons was studied. It was found that the most stable aqueous emulsions of hexadecane and oil in the presence of microorganisms Rhodococcus erythropolis S67 and humic substances of reed fen peat and emulsion in the relation to fuel - humic substances of sphagnum high-moor peat and Rhodococcus erythropolis X5 . It is proved that the obtained biocomposition based on humic substances of peats and microorganisms of the genus Rhodococcus is able to stabilize emulsions of oil hydrocarbons in water by binding them with humic substances of peat to non-toxic complexes.

scholarly journals INFLUENCE OF PEATS HUMIC SUBSTANCES ON THE STATE OF DROPS OF CRUDE OIL AND OIL PRODUCTS IN THE PRESENCE OF OIL DEGRADING MICROORGANISMS OF THE GENUS RHODOCOCCUS

2021 ◽  
pp. 69-79
Author(s):  
Елена Дмитриевна Дмитриева ◽  
Мария Михайловна Герцен
Keyword(s):  
Crude Oil ◽  
Humic Substances ◽  
Rhodococcus Erythropolis ◽  
Petroleum Products ◽  
Water Interface ◽  
High Moor ◽  
Oil Water ◽  
Dispersing Ability ◽  
High Moor Peat ◽  
Oil And Oil Products

Максимальное диспергирование капель модельных нефтепродуктов в присутствии микроорганизмов Rhodococcus erythropolis X5 и гуминовых кислот сфагнового верхового торфа, а также микроорганизмов Rhodococcus erythropolis S67 и гуминовых кислот тростникового низинного торфа. Проведенный экспресс-тест доказал, что уменьшение пленки нефтепродуктов может быть использовано для проверки диспергирующей способности микроорганизмов Rhodococcus X5 или S67 и молекул гуминовых кислот. Установлено, что биокомпозиция на основе гуминовых кислот и микроорганизмов-нефтедеструкторов рода Rhodococcus способна адсорбироваться на границе раздела нефть-вода, переводя нефть в водную толщу, где она разлагается микроорганизмами-нефтедеструкторами. Maximum dispersion of droplets of model petroleum products has been observed in the presence of microorganisms Rhodococcus erythropolis X5 and humic substances of sphagnum high-moor peat, and microorganisms Rhodococcus erythropolis S67 and humic substances of reed fen peat. The express test proved that the reduction of the oil sheen can be used to the test the dispersing ability of microorganisms Rhodococcus X5 or S67 and humic substances. It has been established that a biocomposition based on humic substances and oil degrading microorganisms of the genus Rhodococcus is capable of adsorbing at the oil-water interface, transferring oil into the water column, where it is decomposed by oil degrading microorganisms.

scholarly journals MOLECULAR-MASS DISTRIBUTION OF HUMIC SUBSTANCES AND HYMATOMELANIC ACIDS FROM DIF-FERENT ORIGIN PEATS OF THE TULA REGION

2017 ◽  
pp. 187-194
Author(s):  
Елена (Elena) Дмитриевна (Dmitrievna) Дмитриева (Dmitrieva) ◽  
Мария (Marija) Михайловна (Mihajlovna) Леонтьева (Leontyeva) ◽  
Кристина (Kristina) Викторовна (Viktorovna) Сюндюкова (Siundiukova)
Keyword(s):  
Humic Substances ◽  
Molecular Mass Distribution ◽  
Relative Content ◽  
Alkaline Extraction ◽  
Black Alder ◽  
High Moor ◽  
Molecular Masses ◽  
Different Origin ◽  
Minimum Yield ◽  
High Moor Peat

Humic substances were isolated from different origin peats of the Tula region: reed fen peat (RFP), sphagnum high-moor peat (SHP), black alder fen peat (BFP), sphagnum transition peat (STP) by water-alkaline extraction. The yield of humic substances from reed fen peat is maximal and comes in at 11,1±0,2%; The minimum yield is from sphagnum transition peat 7,4±0,2%, which is explained by the degree of transformation of the initial biomaterial and the conditions of occurrence of peats. Analyzed peats are in a row according to the content of humic substances: RFP>SHP>BFP>STP. The yields of hymatomelanic acids come in at 23,3–25,3%. Polydispersity of humic substances and monodispersity of hymatomelanic acids have been proved by gel-penetrating chromatography. Four fractions differing in their molecular masses and relative content were obtained from humic substances of peats regardless of their origin. Values of the molecular masses of the fractions of HS of BFP are dominant and 25% higher than of the fractions of HS of RFP. Sphagnum peats have a similar qualitative composition and, as a consequence, have similar molecular masses of isolated humic substances correlating with the degree of peat decomposition and with the yields of humic substances. Hymatomelanic acids are one fraction: the molecular weight of GMAs of BFP is 42 kDa, which is 44% higher than of GMAs STP. The correlation between the degree of humification and peat's genesis was revealed. It was shown that the amount of hymatomelanic acids in humic substances of peats can be marker of phases of humification of peats.

Laboratory simulation of the successive aerobic and anaerobic degradation of oil products in oil-contaminated high-moor peat

2015 ◽  
Vol 48 (3) ◽  
pp. 314-324 ◽  
Author(s):  
I. I. Tolpeshta ◽  
S. Ya. Trofimov ◽  
M. I. Erkenova ◽  
T. A. Sokolova ◽  
A. L. Stepanov ◽  
...  
Keyword(s):  
Anaerobic Degradation ◽  
Oil Products ◽  
Laboratory Simulation ◽  
High Moor ◽  
Aerobic And Anaerobic ◽  
High Moor Peat

scholarly journals THE INFLUENCE OF HUMIC ACIDS ON THE SOWING QUALITIES OF COCKWEED IN OIL POLLUTION

2019 ◽  
pp. 349-357
Author(s):  
Elena Dmitriyevna Dmitrieva ◽  
Mariya Mikhaylovna Gertsen ◽  
Svetlana Vladimirovna Gorelova
Keyword(s):  
Humic Acids ◽  
Oil Pollution ◽  
Physiological Activity ◽  
Inhibitory Effect ◽  
Polluted Water ◽  
Black Alder ◽  
Oil Hydrocarbons ◽  
High Moor ◽  
Soil Media ◽  
High Moor Peat

The stimulating effect of humic acids on the processes of growth and development of cress seeds has been revealed. It has been established that use of humic acids of black alder fen peat most effectively affects the sowing qualities of seeds under conditions of oil pollution (an increase in the germination energy and germination capacity compared to the non-humic acid variant by 38%). The minimal inhibitory effect on shoot growth was observed in the soil with hexadecane (20.8 mm less than the control), the maximum – in the soil with oil (22.5 mm less than the control). Humic acids show a stimulating effect on the growth of cress roots under conditions of pollution with oil hydrocarbons. The root length indicators exceeded the values obtained in the version with the pollutant without the use of humic acids from 6% (oil) to 16% of the control (hexadecane). Based on the data obtained during the vegetation experiments, it can be assumed that the stimulating activity of humic acids is more pronounced in stressful conditions for the organism. The biostimulating effect of humic acids was revealed, which manifested itself in an increase in the length of the hypocotyl and root, and the physiological activity of detoxicants was more effectively detected under conditions of pollution with oil hydrocarbons. According to the results of the vegetative experience on a substrate with hexadecane during the irrigation with humic acids of sphagnum high-moor peat, the maximum values of the test responses were determined, corresponding to the control values ​​obtained on the uncontaminated substrate, or higher than the latter. In the variant with oil, the most pronounced effect was achieved with the introduction of humic acids of reed fen and sphagnum high-moor peat, which confirms the hypothesis about the protective effect of humic acids in pollution conditions, which is determined by the binding ability of humic acids in relation to toxicant and their own biological activity in the relation to plants. It is proved that humic acids of peats make a significant contribution to the natural processes of self-purification, causing immobilization and detoxification of petroleum hydrocarbons, which indicates the prospects of their application in technologies of purification and recultivation of polluted water and soil media.

Biologically Active Extractives of High-Moor Peat of Northern European Russia

Vestnik RFFI ◽  
2016 ◽  
Author(s):  
Svtelana B. Selyanina ◽  
◽  
Marina V. Trufanova ◽  
Svtelana A. Zabelina ◽  
Mikhail V. Bogdanov ◽  
...  
Keyword(s):  
European Russia ◽  
Biologically Active ◽  
Northern European ◽  
High Moor ◽  
Northern European Russia ◽  
High Moor Peat

scholarly journals Development of oil product contaminated wastewater treatment technology using sorbents based on mining waste

2020 ◽  
Vol 161 ◽  
pp. 01030
Author(s):  
Lyaysan Araslanova ◽  
Elena Kuznetsova ◽  
Irene Tuktarova ◽  
Alexey Nazarov
Keyword(s):  
Wastewater Treatment ◽  
Oil Products ◽  
Treatment Technology ◽  
Adsorption Activity ◽  
Oil Hydrocarbons ◽  
Processing Plants ◽  
Real Wastewater ◽  
Oil Company ◽  
Degree Of Purification ◽  
Oil And Oil Products

One of the most common types of ecotoxicants is oil products - unidentified group of oil hydrocarbons and products of its processing. In this regard, the development of technology for obtaining new highly efficient and inexpensive sorbents for wastewater treatment from oil products (including industrial waste) is an urgent problem. In this paper, the technology of wastewater treatment includes the use of composite sorbents based on tails-waste of Uchalinsky (UW) and Buribaevsky (BW) mining and processing plants (MPP), mica quartzite (MQ) of the Baymak field and montmorillonite clay (MC) and sodium humates (SH) obtained from waste of Tulgan brown coal. Using these sorbents, we investigated the efficiency of these sorbents in model wastewater treatment contaminated with oil from the Vozeyskoye oilfield of LLC Usinsk Neftegaz and real wastewater of “Bashneft” Ufa Oil Company. It is shown that the efficiency of treatment and the rate of adsorption of model wastewater containing oil products (8-50 mg/dm3) increases in a number of sorbents MQ < BAU < BW (UW) + MC < BW (UW) + MC+SH (BAU is a comparison sorbent - activated carbon) and reaches a maximum for MPP coated with SH. For real wastewater containing oil products of Ufa refinery (3-625 mg/l) adsorption activity increases in the number of MQ < MQ + MC < BW + MC < UW + MC. Thus, the most effective sorbents for real and model wastewater containing oil and oil products are sorbents based on waste from Uchalinsky MPP in the composition of MH (degree of purification 96.1 %), as well as sorbent: waste UW (BW) + MC modified SH (98.1 and 98.2 %, respectively).

scholarly journals Influence of increasing nickel content in soil on Miscanthus × giganteus Greef and Deu. Yielding and on the content of nickel in above-ground biomass / Wpływ wzrastającej zawartości niklu w glebie na plonowanie Miscanthus x giganteus Greef i Deu. i zawartość niklu w nadziemnej biomasie

2015 ◽  
Vol 41 (1) ◽  
pp. 72-79
Author(s):  
Maciej Bosiacki
Keyword(s):  
Nickel Content ◽  
Mineral Soil ◽  
Nickel Sulfate ◽  
First Year ◽  
Above Ground Biomass ◽  
Miscanthus X Giganteus ◽  
Miscanthus Giganteus ◽  
High Moor ◽  
High Nickel ◽  
High Moor Peat

Abstract The aim of the research conducted in a 2-year pot experiment in an unheated plastic tunnel was to determine suitability of Miscanthus × giganteus for phytoextraction of nickel from soil as well as to assess tolerance of this species on increasing concentrations of this metal in soil. Pots were filled with mineral soil (sand) and a mixture of soil with high-moor peat and three levels of nickel were introduced, i.e. 75 mg dm-3, 150 mg dm-3 and 600 mg dm-3 and the control combinations used substrates without the addition of nickel. Nickel was introduced only in the first year of the experiment in the form of nickel sulfate (NiSO4 · 6H2O). Miscanthus × giganteus accumulated a considerable amount of nickel in biomass. Miscanthus × giganteus growing in contaminated mineral soil turned out to be a species tolerant to high nickel concentrations

Development of a New Technology of Environmental Purification from Naphthalene

2015 ◽  
Vol 1085 ◽  
pp. 154-160 ◽  
Author(s):  
Lev V. Nechaev ◽  
Olga N. Tchaikovskaya ◽  
Elizaveta V. Maltseva ◽  
Natalya V. Yudina
Keyword(s):  
Humic Acids ◽  
New Technology ◽  
Luminescent Properties ◽  
Structural Group ◽  
High Moor ◽  
Molecular Masses ◽  
Spectral And Luminescent Properties ◽  
Group Compositions ◽  
The Given ◽  
High Moor Peat

Results of investigation of naphthalene interaction with humic acids of high-moor peat and their fractions are presented in the article. The elemental and structural-group compositions of humic acids of high-moor peat and of their fractions are determined, average values of their molecular masses are found, and their spectral and luminescent properties are investigated. Numerical values of constants of naphthalene interaction with humic acids and their fractions are calculated, and the polarity of naphthalene microenvironment in solutions of humic acids is estimated. The results of investigations suggest that the maximum affinity of naphthalene is observed to the fraction of hematomelanic acids and that the naphthalene microenvironment in solutions of the given fraction is polar.

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