scholarly journals The diagnosis of the autoimmune process in bronchial asthma in children using antigens of the bronchopulmonary system

2018 ◽  
Keyword(s):  
Bronchial Asthma ◽  
Comparison Group ◽  
Morphological Changes ◽  
Diagnostic Value ◽  
Healthy Children ◽  
Autoimmune Process ◽  
Protein Antigens ◽  
Cell Tissue ◽  
Protein Nature ◽  
Asthma In Children

The paper presents data on the detection of an autoimmune process in the bronchopulmonary system in children with BA aged from 5 to 14 years in the period of exacerbation. The comparison group consisted of 25 healthy children aged from 7 to 14 years. It has been shown that lipopolysaccharide antigens from the interstitial connective tissue of bronchopulmonary structures obtained from accidentally killed children have a higher specificity than protein antigens and allow identifying morphological changes in the bronchopulmonary system, the severity of the course and monitoring the effectiveness of the therapy. Antigens obtained from the sectional material of the bronchopulmonary system of protein nature cannot be obtained industrially, since they are not standardized for protein, do not possess sterility, which greatly limits their use and reduces the diagnostic value. The multicomponent antigenic composition of protein homogenates obtained from the cell-tissue structures of the bronchopulmonary system does not clearly identify the nature of the morphological changes in bronchial asthma in children. Lipopolysaccharide antigens from bronchopulmonary structures do not contain protein in their composition; they can be obtained industrially under sterile conditions and stored in a lyophilized state for more than two years, which allows them to be widely used in immunodiagnostics of clinical forms and severity of asthma in children.

Role of autoimmune disorders in the pathogenesis of cerebrovascular insufficiency in children with bronchial asthma

2021 ◽  
Vol 41 (1) ◽  
pp. 37-43
Author(s):  
V. G. Chernusky ◽  
◽  
M. M. Popov ◽  
G. V. Letyago ◽  
O. L. Govalenkova ◽  
...  
Keyword(s):  
Bronchial Asthma ◽  
Cerebral Vessels ◽  
Cerebrovascular Insufficiency ◽  
Autoimmune Process ◽  
Cell Tissue ◽  
Children With Asthma ◽  
Asthma In Children ◽  
Tissue Structures ◽  
The Brain

It has been shown that the main spectrum of immunopathological reactions in bronchial asthma in children has a clear antigenic dependence not only on the inflammatory-activated intermediate stroma of the bronchopulmonary system, but also on the effects of autoantibodies on cerebral vessels and cell tissue. insufficiency of this contingent of children, which is currently insufficiently studied. The aim is to study autoimmune disorders in the pathogenesis of cerebrovascular insufficiency in children with bronchial asthma. 121 patients with asthma aged 5 to 15 years in the period of exacerbation were examined. To study the role of the autoimmune component in the development of cerebrovascular insufficiency and its connection with the autoimmune process in the bronchopulmonary system in asthma in children, we used the method of quantitative determination of autoantibodies to lipopolysaccharide antigens of cerebral vessels and topographic structures of the brain and brain, trachea, bronchi and lung tissue. The results showed that the levels of autoantibodies to lipopolysaccharide antigens vessels and cell tissue structures of the brain and bronchopulmonary system in children with asthma significantly increased from mild to severe. The rank correlation showed that there is a direct reliable connection between the autoimmune process in the bronchopulmonary system and the level of autoantibodies to the lipopolysaccharide antigens of cerebral vessels and cell tissue structures of the brain. Thus, it is shown that the level of autoantibodies to lipopolysaccharide antigens arteries, venous vessels and cell tissue structures of the brain, allows to detect lesions of blood vessels and tissue areas of the brain in cerebrovascular insufficiency in children with asthma.

The value of functional research methods in the diagnosis of pre-asthma in children

1993 ◽  
Vol 74 (4) ◽  
pp. 251-255
Author(s):  
T. V. Klykova ◽  
A. M. Potemkina ◽  
A. G. Zagidullina
Keyword(s):  
Early Diagnosis ◽  
Research Methods ◽  
Bronchial Asthma ◽  
Diagnostic Value ◽  
External Respiration ◽  
Adequate Treatment ◽  
Respiratory Allergies ◽  
Asthma In Children

Respiratory allergies are one of the most common and severe allergic pathologies in children. Our observations showed that bronchial asthma is formed in more than 80% of children through the stage of pre-asthma. However, its early diagnosis and adequate treatment in 90% of cases can prevent the transition to bronchial asthma. In this regard, the problem of studying the methods of early diagnosis of pre-asthma and ways to prevent its transformation into bronchial asthma is urgent and practically significant. Since the formation of pre-asthma and bronchial asthma is based on a violation of the function of external respiration, the purpose of our work was, firstly, to study the function of external respiration in case of pre-asthma, and secondly, to determine the diagnostic value of various methods for studying FVD in this pathology in children.

scholarly journals Peculiarities of the Lifestyle and Psychological State of Primary Schoolchildren with Bronchial Asthma

2017 ◽  
Vol 14 (2) ◽  
pp. 109-114
Author(s):  
Alexandra M. Timofeeva
Keyword(s):  
Bronchial Asthma ◽  
Evaluation Method ◽  
Comparison Group ◽  
Negative Impact ◽  
Psychological Status ◽  
Psychological State ◽  
Healthy Children ◽  
Study Objective ◽  
Treatment Methods ◽  
Primary Schoolchildren

The need to develop an integrated approach to the treatment of children with bronchial asthma is conditioned by a significant increase in morbidity, emergence of more severe forms of the disease and a lack of radical treatment methods. Along with the development and implementation of modern drug treatment methods, the most topical issues are a consideration of the socio-psychological characteristics of patients and psychological and pedagogical support of children during treatment. Study Objective: To study the lifestyle peculiarities of primary schoolchildren (7–10 years) with bronchial asthma and their psychological state. Patients and Methods. The study included children with bronchial asthma (atopic form, partially controlled course) as well as conditionally healthy peers (health groups I and II) as a comparison group. The psychological status of the observed was assessed using Dembo-Rubinstein self-evaluation method, modified «Human Figure» method, and Varga-Stolin parental relationship test. Results. The study involved 75 children (mean age 8.2 ± 0.6 years, including girls 59%, illness duration 3.2 ± 0.5 years) and their parents (mothers made 79% of them). The comparison group consisted of 54 conditionally healthy children (mean age 8.4 ± 0.3, girls 54%) and their parents (mothers — 85%, fathers — 15%). It is shown that the lifestyle of primary schoolchildren changed due to illness distorts the process of personality development and leads to the emergence of difficulties in the emotional and personality sphere: a reduced background of mood (35; 46.6% versus 11; 20%), blockage of experiences and difficulties in their verbalization (56; 75% versus 25; 46%), defensive-aggressive type of behavior in a stress situation (68; 91% versus 25; 46%), pronounced intrapersonal conflict (47; 62% versus 32; 59%), and communication problems (unsociability, restriction in communication, social fears). Conclusion. Bronchial asthma changes the child's lifestyle, has a negative impact on the psychological state and personality becoming. It is possible to prevent the emergence of negative social consequences of the disease, to smooth out their severity by providing psychological and pedagogical assistance to a child and his family from the time the disease is detected and throughout all treatment stages. The creation and implementation of psychological support programs for families raising children with bronchial asthma at various treatment stages are extremely important and represent a further research direction.

scholarly journals ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN

2018 ◽  
Vol 20 (6) ◽  
pp. 905-912
Author(s):  
A. A. Lebedenko ◽  
T. P. Shkurat ◽  
E. V. Mashkina ◽  
O. E. Semernik ◽  
T. K. Dreyzina ◽  
...  
Keyword(s):  
Bronchial Asthma ◽  
Polymorphic Locus ◽  
Severe Disease ◽  
Clinical Manifestations ◽  
Control Group ◽  
Moderate Degree ◽  
Healthy Children ◽  
Heterozygous Locus ◽  
Children With Asthma ◽  
Asthma In Children

In the present study, we have examined association between different polymorphic variants of metalloproteinases genes and clinical manifestations of bronchial asthma in children. We observed 103 patients including 42 children with an established diagnosis of asthma. Moreover, 61 persons were examined in the control group. All patients underwent genetic testing by allele-specific polymerase chain reaction. In particular, 320A>C polymorphic locus of ММР20 gene; Val275Ala ММР20, and -8202A>G gene ММР9 were analyzed.We have found that 30 patients (71.4% of total) had bronchial asthma of mild severity, 9 children (21.4%) exhibited moderate degree, and 3 patients (7%) had severe-grade disease. Homozygous C/C variant of the polymorphic ММР20 gene, 320A>C heterozygous variant of the ММР20 Val275Ala polymorphism, and heterozygous locus of -8202A>G ММР9 gene were found to be most frequent among the children with asthma. Generally, we have observed that the frequencies of the studied alleles and genotypes did not significantly differ berween the asthma patients and children from the control group (p < 0.05). However, in patients with GGgenotype of -8202A>G ММР9 polymorphism combined with homozygosity for the C allele of ММР20 320A>C, a more severe disease was observed, being combined with polyvalent sensitization and high total IgE levels in blood serum.In conclusion, frequencies of alleles and genotypes among patients with asthma did not show any statistically significant differences from the group of healthy children. The patients homozygous for G allele of ММР9 -8202A>G polymorphism gene and for the C allele ММР20 gene (320A>C) seem to be predisposed for a more severe clinical course of the disease. 

scholarly journals The significance of the distribution of the indicators of humoral and cell immunity and their role in the pathogenesis of bronchial asthma in children with the position of system analysis

2021 ◽  
Keyword(s):  
Bronchial Asthma ◽  
System Analysis ◽  
Sustainable Growth ◽  
Immunological Parameters ◽  
Clinical Forms ◽  
Cell Immunity ◽  
Group I ◽  
Cell Tissue ◽  
Asthma In Children ◽  
Tissue Structures

Bronchial asthma is one of the common diseases in children of different ages. In recent years, around the world, including in Ukraine, there is a trend towards its sustainable growth. To date, there are no methods of systemic immunodiagnostics that would allow with high diagnostic accuracy to identify clinical forms and severity of asthma, which would allow more fully reveal the pathogenetic mechanisms and individualize approaches to the treatment of asthma in children. The aim of this work was to study the hierarchy of immunological parameters in the pathogenetic matrix, which will determine the features of clinical forms and severity of asthma in children on the basis of systematic analysis. A comprehensive clinical and immunological examination of 176 children with asthma aged 6 to 15 years. To detect the autoimmune component used lipopolysaccharide antigens obtained from homologous cell-tissue structures of the trachea, bronchi and lung tissue from sectional samples of the bronchopulmonary system from accidentally killed children with group I (0) blood 2–4 hours after death. The level of autoantibodies to lipopolysaccharide antigens of the bronchopulmonary system was determined by quantifying the autoantibody index – Qφ. As a result of the study for the first time to improve the diagnosis and differentiation of clinical forms and severity of asthma from the standpoint of system analysis was developed immunodiagnostic complex, which took into account the degree of deviation from the norm values (Student's t-test, t = 1.96) and their distribution in pathogenetic matrix. This approach to ranking the positions of immunological parameters allowed to determine the features of humoral and cellular immunity, the process of apoptosis of cell-tissue structures of the bronchopulmonary system and the autoimmune component in the pathogenesis of asthma in children, which opens approaches to individualization of pathogenetic therapy.

scholarly journals ACID-BASIC BALANCE, BLOOD GASES AND ECHOCARDIOGRAPHIC CHANGES IN BRONCHIAL ASTHMA IN CHILDREN

2020 ◽  
pp. 565-571
Author(s):  
K.I. ISMOILOV ◽  
◽  
M.M. SHARIPOVA ◽  
Keyword(s):  
Bronchial Asthma ◽  
Respiratory Acidosis ◽  
Blood Gases ◽  
Control Group ◽  
Healthy Children ◽  
Acid Base Balance ◽  
Acid Base ◽  
Significant Difference ◽  
Base Balance ◽  
Asthma In Children

Objective: To examine the acid-base balance, blood gases and echocardiographic changes in bronchial asthma in children. Methods: The results of the examination of 60 children suffering from bronchial asthma (BA), aged from 5 to 14 years are presented. The patients were divided into 2 groups depending on their age. The first group consisted of 28 children from 5 to 10 years old, the second – 32 children aged 11 to 14 years. The control group included 20 healthy children, identical to the surveyed groups in terms of gender and age. Echocardiography was performed on a Vivad 3 apparatus (GE, USA), the acid-base balance and blood gases were studied on an ABL 800 Flex apparatus (Radiometer Medical, Denmark). Results: Studies have shown that children with asthma had significant changes in the ventilation capacity of the lungs in the form of perfusion and diffusion disorders, which undoubtedly influenced the severity of their condition. In children of both groups, there was a significant lack of bases (p<0.001) and a decrease of pH average value in comparison with similar indicators of healthy children. A significant difference in BE and pH values was found between the two groups of patients (p<0.01). The main changes in hemodynamics were reflected in the indicators of TV A and TV E/A. As in children under 10 years of age with BA, and in children over 10 years of age with BA, TV A was higher than in children of the control group (0.48±0.02 versus 0.38±0.02 (p<0.05) and 0.59±0.02 versus 0.49±0.02 (p<0.05) respectively), while the TV E/A ratio was lower in children with BA (1.22±0.03 versus 1.42±0.05 (p<0.05) and 1.31±0.04 versus 1.59±0.06 (p<0.05) respectively). Conclusions: It was found that third patients in the interictus period of the disease have mild hypoxemia, which, as a rule, is combined with a drop in the alveolar-arterial O2 gradient. The examined patients were characterized by respiratory acidosis. The detected echocardiographic changes confirmed the presence of myocardial dystrophy and the development of «cor pulmonale» in 2 children of the second group with more than 6 years and occurring frequent exacerbations. Keywords: Bronchial asthma, children, heart, echocardiography, acid-base balance, blood gases.

Physical Rehabilitation of Children with Bronchial Asthma

2020 ◽  
Vol 98 (4) ◽  
pp. 20-25
Author(s):  
N.A. Lyan ◽  
E.A. Turova ◽  
I.I. Ivanova ◽  
I.A. Bokova ◽  
I.I. Kalinovskaya
Keyword(s):  
Bronchial Asthma ◽  
High Frequency ◽  
New Technologies ◽  
Comparison Group ◽  
Clinical Course ◽  
Bronchial Tree ◽  
Medical Rehabilitation ◽  
Disease Course ◽  
Moderate Disease ◽  
Asthma In Children

The presence in children with bronchial asthma of disorders of bronchial patency, hypersecretion, deterioration of the drainage function of the bronchi determines the need to develop new technologies of kinesotherapy aimed at cleansing the bronchial tree from viscous sputum, strengthening the chest muscles. In this regard, the use of high-frequency thoracic oscillation is promising. Purpose. Study of the effectiveness of high frequency chest oscillation in children with bronchial asthma. Materials and methods. 60 children with bronchial asthma aged 6 to 17 years were examined. The main group was 30 children who received exposure to high frequency chest oscillation, the comparison group included 30 children who received only basic therapy, including basic anti-asthma therapy and respiratory gymnastics. Results. In the course of the study, positive dynamics of clinical-functional indicators was revealed, characterized by a decrease in the frequency of wet and dry seizure cough and its disappearance, increased sputum discharge, normalization of the auscultal picture in the lungs against the background of improving the performance of external breathing and respiratory excursion of the chest. More pronounced dynamics were observed in children with a moderate disease course, who were in incomplete remission and had initially lower computer flowmetry rates. Conclusion. Based on the studies, the possibility of using high-frequency chest oscillation in the comprehensive medical rehabilitation of children with bronchial asthma was scientifically substantiated. Peculiarities of action of high-frequency thoracic oscillation on clinical course of bronchial asthma characterized by improvement of bronchial patency, enhancement of sputum withdrawal, improvement of mobility of ribs, diaphragm, and biomechanics of respiration are studied. High therapeutic effectiveness of high-frequency chest oscillation in the treatment of bronchial asthma in children was established – 93.3%, which is significantly higher than in the comparison group (73.3%, p < 0.05).

The Potential Role of The Long non-coding RNA MEG3 in Bronchial Asthma in children

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Basma Tarek Rezk ◽  
Fayda Ibrahim Abdel Motaleb ◽  
Terez Boshra Kamel ◽  
Enas Samir Nabih ◽  
Marwa Ali Abd El-Khalek
Keyword(s):  
Bronchial Asthma ◽  
Airflow Obstruction ◽  
Response To Therapy ◽  
Control Group ◽  
Healthy Children ◽  
Asthmatic Children ◽  
Clinical Biomarkers ◽  
Asthma In Children ◽  
Reversible Airflow Obstruction

Abstract Aim and Background Asthma is an inflammatory disease affecting the airways of the lungs being characterized by reversible airflow obstruction and bronchospasm. Environmental and genetic factors play a role in the pathogenesis of the disease; these factors are controlled by epigenetic mechanisms. There is a demand of clinical biomarkers for diagnosis of asthma and monitoring the response to therapy. In this study, we aimed to evaluate the role of lnc-MEG3 expression and its downstream effector in the pathogenesis of bronchial asthma disease. Methods Based on bioinformatics analysis using online databases, the current work has been designed to study the expression status of lnc-MEG3 and GDF15 mRNA in serum and sputum of asthmatic children aiming to find its clinical significance. This study was conducted on 30 children, 15 children newly diagnosed with bronchial asthma as patient group (A) and 15 age and sex matched healthy children as control group (B). Quantitative PCR was carried out to evaluate the expression pattern of selected biomarkers in serum and sputum of asthmatic children. Results The expression of circulating lnc-MEG3 was highly significantly up-regulated in asthmatic children than in control group (p ≤ 0.01), while the expression of circulating GDF15 mRNA was highly significantly down-regulated in asthmatic children than in control group (p ≤ 0.01). The findings showed a negative significant correlation between both investigated biomarkers. Conclusion This study supports the role of lnc-MEG3 and GDF15 protein as potential biomarkers in diagnosis of bronchial asthma in children.

Qualitative and quantitative assessment of nailfold capillaries by capillaroscopy in healthy volunteers

VASA ◽  
2012 ◽  
Vol 41 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Hoerth ◽  
Kundi ◽  
Katzenschlager ◽  
Hirschl
Keyword(s):  
Scoring System ◽  
Morphological Changes ◽  
Connective Tissue Diseases ◽  
Capillary Density ◽  
Diagnostic Value ◽  
Healthy Individuals ◽  
Extreme Position ◽  
Qualitative And Quantitative ◽  
Diffuse Loss ◽  
Density Results

Background: Nailfold capillaroscopy (NVC) is a diagnostic tool particularly useful in the differential diagnosis of rheumatic and connective tissue diseases. Although successfully applied since many years, little is known about prevalence and distribution of NVC changes in healthy individuals. Probands and methods: NVC was performed in 120 individuals (57 men and 63 women; age 18 to 70 years) randomly selected according to predefined age and sex strata. Diseases associated with NVC changes were excluded. The nailfolds of eight fingers were assessed according to standardized procedures. A scoring system was developed based on the distribution of the number of morphologically deviating capillaries, microhaemorrhages, and capillary density. Results: Only 18 individuals (15 %) had no deviation in morphology, haemorrhages, or capillary density on any finger. Overall 67 % had morphological changes, 48 % had microhaemorrhages, and 40 % of volunteers below 40 years of age and 18 % above age 40 had less than 8 capillaries/mm. Among morphological changes tortous (43 %), ramified (47 %), and bushy capillaries (27 %) were the most frequently altered capillary types. A semiquantitative scoring system was developed in such a way that a score above 1 indicates an extreme position (above the 90th percentile) in the distribution of scores among healthy individuals. Conclusions: Altered capillaries occur frequently among healthy individuals and should be interpreted as normal unless a suspicious increase in their frequency is determined by reference to the scoring system. Megacapillaries and diffuse loss of capillaries were not found and seem to be of specific diagnostic value.

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