Prevalence and antibiotic sensitivity of pathogens isolated in urinary tract infection in a medical college hospital

Background : Urinary tract infection (UTI) is one of the most important causes of morbidity and mortality in the developing countries like Bangladesh. Antimicrobial agents are the frequently used drug for its treatment. Periodic evaluation of antimicrobial activity of different antibiotics is essential as the pattern of antibiotic sensitivity may vary over periods. Increasing antibiotic resistance among urinary pathogens to commonly prescribed drugs has become a global reality today.Objective : To determine the prevalence and to find out the causative agents of UTI and their antibiotic sensitivity pattern among suspected UTI patients attending Northern International Medical College Hospital (NIMCH), Dhaka.Methods : A retrospective study was conducted at Northern International Medical College Hospital, Dhaka, Bangladesh by analyzing the records of urine samples collected for culture and sensitivity tests over a period of six months (January 2014 to June 2014). Of the total 878 clean catch mid-stream urine samples collected from suspected cases of UTI patients of all ages and both sexes. Urine specimens were cultured for isolation of microbial agents of urinary tract infection. The isolated bacteria were identified using biochemical test. The diffusion susceptibility test was used to determine susceptibility of bacterial agents to antibiotics. Computerized data was collected from Microbiology department of NIMCH and analyzed by Microsoft Excel Version 2013.Results : In this study, 182 (20.73%) out of 878 urine sample were positive for pathogenic organisms. Of the various pathogenic organisms isolated, Escherichia coli constituted for 85.16% followed by Pseudomonas sp, Acinatobactersp, Group D Streptococcus, Staphylococcus aureus, Klebsiellasp, Enterobactersp and others. E.coli was found to be most sensitive to Imipenem, Amikacin and Meropenem and resistant to most commonly used oral drugs like Azithromycin, Cefexime, cotrimoxazole and Ciprofloxacin and Levofloxacin.Conclusion : Mainly Gram negative bacilli is responsible for UTI and most frequent isolated bacteria was E-coli. The most effective antibiotics were Imipenem, Amikacin, Meropenem,all of them are parentral. Majority E-coli were resistant to commonly used oral drugs like Azithromycin, Cefexime, cotrimoxazole and Ciprofloxacin. Therefore the choice of antibiotic therapy in UTI should be depends on the local sensitivity pattern of the infecting organisms.Northern International Medical College Journal Vol.7(1) Jul 2015: 105-109

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Antibiotic resistance pattern of bacteria causing urinary tract infection in a private medical college hospital, Dhaka

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v16i1.31131 ◽

2017 ◽

Vol 16 (1) ◽

pp. 42-47

Author(s):

Mahmuda Siddiqua ◽

Ahmed Nawsher Alam ◽

Sonia Akter ◽

Reena Saad Ferdousi

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Medical College Hospital ◽

Resistance Pattern ◽

College Hospital ◽

E Coli ◽

Medical College ◽

Tract Infections ◽

Resistance Rates

Background and objective: Urinary tract infection (UTI) is one of the frequently seen infections both in the world and in our country as well. Escherichia coli (E. coli) aremost frequently isolated in complicated or uncomplicated, nosocomial or community acquired urinary tract infections. To guide the empirical therapy, the resistance pattern of E. coli responsible was evaluated throughout the period in this study.Material and Method: Urine samples from outpatient / inpatient department of Ibn Sina Medical College Hospital between 1st January, 2015 and 31st December, 2015 were retrospectively analyzed. Presence of ?105 colony forming units/ml in urine culture was considered as significant for UTI. Isolated bacteria were identified by standard laboratory techniques and antibiotic susceptibility testing was performed by Kirby-Bauer disk diffusion method using Clinical Laboratory Standard Institute (CLSI) criteria.Result: A total of 271(13.4%) uropathogens were isolated. Overall E. coli accounted for 180 (66.42%) of all isolates. Resistance rates of E. coli to antimicrobial agents was demonstrated to be as follows: cefuroxime 82%, nalidaxic acid 74%, azithromycin 56%, cefotaxime52%, ceftazidime50%, cefixime 47%, cotrimoxazole 43%, ceftriaxone 41%, ciprofloxacin38%, amoxicillin- clavulanic acid 31%, cefepime30%, and low resistance which ranges from 9 to 1% included gentamycin 9%, meropenem 3%, imipenem2%, nitrofurantoin2% and amikacin 1%.Conclusion: As resistance rates show regional differences, it is necessary to regularly monitor regional resistance pattern to determine the appropriate empiric antibiotic treatment. The national antibiotic usage policies must be reorganized according to data obtained from these studies.Bangladesh Journal of Medical Science Vol.16(1) 2017 p.42-47

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Urinary Tract Infection in Nephrotic Syndrome - A study of 62 cases at Faridpur Medical College Hospital

Faridpur Medical College Journal ◽

10.3329/fmcj.v13i1.38017 ◽

2018 ◽

Vol 13 (1) ◽

pp. 35-39

Author(s):

Lakshman Chandra Kundu ◽

Aloke Kumar Saha ◽

Md Kamrul Hassan ◽

Antara Kundu

Keyword(s):

Urinary Tract Infection ◽

Nephrotic Syndrome ◽

Urinary Tract ◽

Tract Infection ◽

White Blood Cells ◽

Medical College Hospital ◽

College Hospital ◽

Serum Triglycerides ◽

Medical College ◽

Study Population

Urinary tract infection is a common complication of Nephrotic syndrome, many cases remain asymptomatic. This study was aimed to determine the possible underlying causes of urinary tract infection in Nephrotic syndrome. The study population in this descriptive study included children aged six months to fourteen years old with Nephrotic syndrome referred to Paediatric ward of Faridpur Medical College Hospital from June 2015 to March 2016. The mean age was 6.12±3.25 years in UTI group and 7.26±3.39 years in without UTI group. Male were predominant in both groups, 15(60.0%) in UTI group and 24(64.9%) in without UTI group. Serum albumin, total protein, urinary protein excretion, the number of white blood cells, hemoglobin, erythrocyte sedimentation rate, blood creatinine, nitrogen, blood urea, serum triglycerides were not statistically significant between the groups. The most common microorganisms involved in urinary tract infection were: Escherichia coli (13%) & Klebsiella (13%). Majority 29(46.8%) patients had fever, 12(19.4%) had diarrhea, 11(17.7%) had bad smell of urine, 10(16.1%) had vomiting associated with diarrhea and 10(16.1%) had respiratory symptoms. Children with nephrotic syndrome are frequently predisposed to urinary tract infection and in most cases it is asymptomatic, often undiagnosed. E. coli is the commonest organism causing UTI.Faridpur Med. Coll. J. Jan 2018;13(1): 35-39

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Prevalence of Urinary Tract Infection in Patient with Indwelling Catheter in Chittagong Medical College Hospital

Chattagram Maa-O-Shishu Hospital Medical College Journal ◽

10.3329/cmoshmcj.v17i1.39442 ◽

2018 ◽

Vol 17 (1) ◽

pp. 42-45

Author(s):

Raushan Akter ◽

Mahtab Uddin Hassan ◽

Rajat Sankar Roy Biswas

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Statistical Method ◽

Medical College Hospital ◽

Indwelling Catheter ◽

College Hospital ◽

Medical College ◽

Positive Data ◽

Hospital Acquired

Background: Hospital acquired urinary tract infection among the hospitalized catheterized patient due to different indications are a common morbility. The objective of study to see the prevalence hospital acquired urinary tract infection among the patients of indwelling catheter admitted in the department Medicine of Chittagong Medical College Hospital. Methods: It was a hospital based obsevational study conducted on purposively selected (Non-probability) hospitalized patients who got indwelling catheter had admitted into the Medicine ward, Chittagong Medical College Hospital from 1st January 2013 to 30th June 2013. Total 50 patients were selected. Urine was analyzed for growth of organism and sensitivity was also done in case where growth was positive. Data was analyzed by statistical method with the software SPSS-20. Results: Results showed that 15 (30%) developed bacteriuria or urinary tract infection with catheter. Development of bacteriuria was not affected by sex, age. People who introduce the catheter has some influence with the occurrence of UTI. E.coli, Klebsella, Proteas and Pseudomonas aeroginosa were the most common bacteria isolated. Imepenam was found the most sensitive antibiotic. Conclusion: Catheter play an important risk for occurrence of UTI. So it should be avoided if possible. Chatt Maa Shi Hosp Med Coll J; Vol.17 (1); Jan 2018; Page 42-45

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Antimicrobial susceptibility test of pathogens isolated from urinary tract infection suspected cases

Janaki Medical College Journal of Medical Science ◽

10.3126/jmcjms.v2i1.11393 ◽

2014 ◽

Vol 2 (1) ◽

pp. 28-34

Author(s):

K Yadav ◽

S Prakash ◽

RC Serayi ◽

T Shilpkar ◽

S Shrestha

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Pathogenic Bacteria ◽

Antibiotic Sensitivity ◽

Medical Problem ◽

Medical Sciences ◽

Medical College ◽

Citrobacter Diversus ◽

Effective Drugs

Background and objectives: Urinary tract infection (UTI) is associated with multiplication of organisms in urinary tract and is defined by the presence of more than 105 organisms per ml in a midstream sample of urine (MSU). UTI is most commonly acquired bacterial infection in ambulatory and hospitalized populations. E.coli is the most predominant organism to colonise the urethral meatus and perineum before ascending to the bladder. Drug resistance of pathogens is a serious medical problem, because of very fast arise and spread of mutant strains that are insusceptible to medical treatment of UTI. Therefore, this study was carried out to determine the common pathogenic bacteria causing UTI and to determine their antibiotic susceptibility pattern. Material and Methods: Mid stream urine samples of the UTI suspected pateints were collected in the Mid Stream Urine (MSU) samples were collected in the sterile clean dry wide mouthed bottle. Standard protocol was followed to isolate and identify organism which was followed by disc diffusion antibiotic sensitivity tests. Results: A total of 100 samples were collected. Out of 100 samples, 25 samples showed a significant growth E.coli, Citrobacter diversus, Klebsiella pneumoniae, Staphylococcus aureus were isolated. E.coli (84%) was found to be the most prevalent causing UTI. Conclusion: UTI was found more common in female than male and E.coli was found to be main causes of UTI. Ciprofloxacin showed more effective drugs in the treatment of UTI. DOI: http://dx.doi.org/10.3126/jmcjms.v2i1.11393 Janaki Medical College Journal of Medical Sciences (2014) Vol. 2 (1): 28-34

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Urinary Tract Infection and Drug Susceptibility Pattern in Patients of a Medical College Hospital in Bangladesh

Journal of Enam Medical College ◽

10.3329/jemc.v4i1.18064 ◽

2014 ◽

Vol 4 (1) ◽

pp. 21-25

Author(s):

Rafiul Alam Khan ◽

Md Fazhul Karim

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Drug Susceptibility ◽

Susceptibility Pattern ◽

Isolation And Identification ◽

College Hospital ◽

Klebsiella Species ◽

Medical College ◽

Single Organism

Background: Urinary tract infection (UTI) is a common and frequently encountered serious morbidity that afflicts the tool not only to all segments of human population but also results in increasing antibiotic resistance due to persistence and mismanagement of the ailment. The present study aimed to ascertain the current situation of antimicrobial resistance of urinary tract infection. Objectives: To find out pyuria by direct microscopy, isolation and identification of the organisms by culture and to know the susceptibility pattern of organisms causing urinary tract infection. Materials and Methods: A total of 180 midstream urine samples were subjected to aerobic bacteriologic culture in the department of Microbiology, Coxs Bazar Medical College and 250-bedded Sadar Hospital, Coxs Bazar during the period of March to October 2012. Specimens were collected from hospitalized and outdoor patients of different age and sex groups. All specimens were examined by routine microscopy to find out significant pyuria (>5 pus cells/HPF). Strict aseptic precautions were taken all through the culture system. Results: Out of 180 specimens, 101 (56.11%) culture yielded significant growth of single organism and 79 (43.89%) yielded no growth. The isolated organisms were E. coli 74.26%, Klebsiella species 12.87%, Enterococci 4.95%, Staphylococcus saprophyticus 3.96%, Pseudomonas species 1.98% and Proteus species 1.98%. The highest sensitivity was shown by imipenem (100%) followed by ceftriaxone (65%), azithromycin (65%), ciprofloxacin (60%) and less sensitive to amoxycillin, cotrimoxazole, cephradine and nalidixic acid ranging 1530%. Conclusion: The findings of the present study recommends that UTI should be treated by selective antibiotics obtained from culture and sensitivity test to minimize increasing trend of drug resistance. DOI: http://dx.doi.org/10.3329/jemc.v4i1.18064 J Enam Med Col 2014; 4(1): 21-25

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PREVALENCE OF URINARY TRACT INFECTION IN CHILDREN (AGE 1 MONTH TO 5 YEARS) WITH FEVER ADMITTED IN JHALAWAR MEDICAL COLLEGE, JHALAWAR

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v4i5.1123 ◽

2020 ◽

Vol 4 (5) ◽

Author(s):

Rakesh Kumar Sharma ◽

Kapil Kumar ◽

Anita Devi

Keyword(s):

Urinary Tract Infection ◽

Preschool Children ◽

Urinary Tract ◽

Tract Infection ◽

Tertiary Care ◽

Tertiary Care Centre ◽

College Hospital ◽

Cross Sectional ◽

Medical College ◽

Culture Positive

Background: The present study is undertaken to estimate the prevalence of urinary tract infection in febrile preschool children (1 month to 5 years of age) in febrile children visiting at tertiary care centre of Jhalawar, Rajasthan Methods: It was a cross-sectional, prospective, observational, non interventional study, carried out to analyse the prevalence of urinary tract infection in febrile preschool children (1 month to 5 years of age) in febrile children visiting medical college, Hospital Jhalawar. The study was conducted in Department of Paediatrics, between Dec. 2019 to March 2020. The study was approved by Ethics Committee. Results: During the study period, we have screened total 1379 preschool children (under 5 years of age) with fever. Out of this total 1379 febrile children, 86 children found to be culture positive cases for UTI (CP-UTI). The prevalence of culture positive cases for UTI in this study was 6.23%. In the prospect of age, 24 (27.91%) children were found to be infant and 62(72.09%) children were found to be of age between 1 years to 5 years. Conclusion: UTIs in preschool children are often having vague and variable symptoms, often fever is the only symptoms. An untreated UTI can lead to subsequent damage and impairment of renal structure and function, it is very important to diagnose and treat UTI in preschool children. Keywords: Urinary tract infection, E.coli, Fever.

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Antibiotic Sensitivity of Bacteria Causing Urinary Tract Infection

Bangladesh Journal of Infectious Diseases ◽

10.3329/bjid.v2i1.31324 ◽

2017 ◽

Vol 2 (1) ◽

pp. 13-18

Author(s):

Rezina Parveen ◽

Md. Abdullah Yusuf ◽

Ishrat Sharmin ◽

Md. Saiful Islam ◽

Ina Rahim

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Antimicrobial Agents ◽

Antibiotic Sensitivity ◽

Diffusion Method ◽

College Hospital ◽

Antimicrobial Sensitivity ◽

Sensitivity Pattern

Background: Urinary tract infection is very common in both male and female.Objectives: The purpose of the present study was to see the antibiotic sensitivity pattern of isolated from urinary tract infected patients.Methodology: This cross sectional study was carried out in Dhaka Medical College Hospital, Dhaka for a period of 12 months. Clinically diagnosed cases of urinary tract infection irrespective of age and sex having pus cells ?5/HPF in the deposits of centrifuged urine were selected as study population. Data regarding organisms causing UTI and their antibiotic sensitivity patterns were collected. For urine culture the urine samples were inoculated on HiCrome UTI agar, CLED agar, 5% sheep blood agar and MacConkeys agar media with a calibrated loop having diameter of 1.45 mm which contains 0.001 ml of urine. The inoculation at 37o C for 24 hours and CFU count of 105/ml of urine were considered positive for UTI. Identification of bacteria was done by standard biochemical techniques and their distinct colony characteristics. All the isolated organisms were tested for antimicrobial sensitivity against different antimicrobial agents by disc diffusion method on Mueller-Hinton agar plates.Result: Diagnosis of bacteria causing UTI with their sensitivity to different antibiotics was performed with a total of 300 samples from both male (38.66%) and female (61.33%) of different age groups. Among 300 samples 107 strains were isolated. Out of 107 identified strains, 95(31.67%) samples showed single growth and 6(2%) samples showed mixed growth. Escherichia coli (64.49%) was found to be the predominant organism. Regarding antimicrobial sensitivity pattern Esch. coli showed 98.55 to 63.77% sensitivity to imipenem, amikacin, ceftazidime and nitrofurantoin. Other isolated organisms showed 50 to 100% sensitivity to ceftazidime, amikacin, imipenem except Klebsiella, Pseudomonas and enterococci spp. which showed 40% and less sensitivity.Conclusion: In conclusion Escherichia coli is the most commonly isolated bacteria which is highly sensitive to imipenem.Bangladesh J Infect Dis 2015;2(1):13-18

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Isolation and Antimicrobial Susceptibility Pattern of Escherichia coli Causing Urinary Tract Infection in Enam Medical College Hospital

Journal of Enam Medical College ◽

10.3329/jemc.v1i2.11463 ◽

2012 ◽

Vol 1 (2) ◽

pp. 60-62 ◽

Cited By ~ 1

Author(s):

Mejbah Uddin Ahmed ◽

Md Khairuzzaman ◽

Afroza Begum ◽

Iftikhar Ahmed

Keyword(s):

Escherichia Coli ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Antimicrobial Resistance ◽

Tract Infection ◽

Resistance Pattern ◽

Susceptibility Pattern ◽

College Hospital ◽

Medical College ◽

Antimicrobial Susceptibility Pattern

Background: Since antibiotic resistance of uropathogens has gradually been rising, knowledge of antimicrobial resistance pattern of Escherichia coli (Esch. coli), the predominant pathogen of urinary tract infection (UTI), is important in selecting empirical antimicrobial therapy.Objectives: To find out the common organisms causing UTI with their antimicrobial susceptibility pattern in Enam Medical College Hospital.Materials and Methods: This study was carried out in the department of Microbiology, Enam Medical College Hospital, Savar, Dhaka between January 2010 to June 2011 to see the antimicrobial resistance pattern of Esch. coli in urine sample. A total of 512 urine samples were studied from clinically suspected UTI cases irrespective of age and sex. Esch. coli were isolated and antibiogram of the isolates were done.Results: The results of antibiogram revealed the resistance pattern of the isolated Esch. coli to ampicillin (93.94%), cefradine (70.71%), trimethoprim-sulfamethoxazole (48.49%), azithromycin (41.42%), ciprofloxacin (39.40%), ceftazidime (35.56%), nitrofurantoin (29.30%), ceftriaxone (10.11%), gentamicin (7.08%) and imipenem (3.04%).Conclusion: It can be inferred that our findings will render useful information to clinicians in determining the appropriately directed antimicrobial regimen when given empirically. DOI: http://dx.doi.org/10.3329/jemc.v1i2.11463 J Enam Med Col 2011; 1(2): 60-62

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Short term catheterization versus long term catheterization after vaginal prolapsed surgery: A randomized control trial in Dhaka National Medical College & Hospital

Journal of Dhaka National Medical College & Hospital ◽

10.3329/jdnmch.v18i2.16012 ◽

2013 ◽

Vol 18 (2) ◽

pp. 4-8

Author(s):

Mst Hosna Ara Khatun ◽

Jahanara Arzu ◽

Zulfe Ara Haider

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Residual Volume ◽

Short Term ◽

College Hospital ◽

National Medical College ◽

Medical College ◽

National Medical

Objective: This study was undertaken to determine whether short term bladder catheterization would be more beneficial than the routinely practiced long term catheterization after vaginal hysterectomy for prolapsed uterus. Materials and methods: This randomized controlled trial was conducted in Obstetrics and Gynecology Department of Dhaka National Medical College Hospital from January 2009 to January 2011. A total of 106 women were included in this study. They were randomized into short term catheterization group where transurethral catheter was removed after 24 hours of surgery and long term catheterization group where catheter was removed on 3rd post operative day. Result: Mean time of first voiding after removal of catheter was almost equal in both groups (2.82±1.42 hours and 2.74±1.52 hours). Duration of catheter did not affect the duration of first voiding time after removal of catheter (P>0.05). None of the women had residual volume of urine >200 ml in both groups. Mean residual volume is significantly high in long term catheterization group (P<0.05). Urinary tract infection was also significantly high in long term catheterization group (P<0.05) and majority of the infection were caused by E. coli. Conclusion: Short term catheterization is more beneficial in terms of lower incidence of urinary tract infection and prevention of bladder over filling as compared to long term catheterization after vaginal prolapsed surgery. DOI: http://dx.doi.org/10.3329/jdnmch.v18i2.16012 J. Dhaka National Med. Coll. Hos. 2012; 18 (02): 4-8

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