scholarly journals Effect of milk fat content on absorption of calcium carbonate and calcium phosphate mixture in Sprague Dawley rats

Food Research ◽  
2019 ◽  
Vol 4 (3) ◽  
pp. 623-627
Author(s):  
Hardoko ◽  
Jasmine V. ◽  
Eveline ◽  
Y. Halim ◽  
L. Antono
Keyword(s):  
Calcium Carbonate ◽  
Calcium Phosphate ◽  
Milk Fat ◽  
Calcium Absorption ◽  
Fat Content ◽  
Sprague Dawley ◽  
High Fat ◽  
Low Fat ◽  
Sprague Dawley Rats ◽  
Absorption Level

Calcium absorption of standard milk is influenced by types of fat, other than types of calcium salts, vitamin D, PTH hormone, etc. However, calcium absorption on different fat content, particularly on milk, has not been reported. The purpose of this study was to determine the effect of the fat content of milk on the absorption of calcium carbonate and calcium phosphate mixture in rats. Experiments were conducted using 24 male Sprague Dawley rats fed with low-fat milk (0.5% fat), high-fat milk (26% fat), standard milk (8% fat) and without milk for three weeks. Amount of feed consumed, feces and urine samples were collected to determine the amount of absorbed calcium for five days in the third week. Calcium content in the samples was analyzed using AAS (Atomic Absorption Spectrophotometry). The results showed that there were no difference in calcium carbonate and calcium phosphate absorption among rats fed with low-fat milk, high-fat milk and standard milk. The calcium absorption level from milk reached an average of around 98.67±0.22%. The absorption level of calcium carbonate and calcium phosphate mixture from milk was higher than control rats that were not fed with any milk. Fat content in milk did not affect the absorption of calcium carbonate and calcium phosphate mixture, but the milk consumption could increase the calcium absorption, compared to control.

Effects of a Long-Term High-Fat Diet and Switching from a High-Fat to Low-Fat, Standard Diet on Hepatic Fat Accumulation in Sprague-Dawley Rats

2008 ◽  
Vol 53 (12) ◽  
pp. 3206-3212 ◽  
Author(s):  
Katsuhisa Omagari ◽  
Shigeko Kato ◽  
Koichi Tsuneyama ◽  
Chisato Inohara ◽  
Yu Kuroda ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Standard Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Low Fat ◽  
Fat Accumulation ◽  
Sprague Dawley Rats ◽  
Hepatic Fat

scholarly journals Differential effects of low-fat and high-fat diets on fed-state hepatic triacylglycerol secretion, hepatic fatty acid profiles, and DGAT-1 protein expression in obese-prone Sprague–Dawley rats

2014 ◽  
Vol 39 (4) ◽  
pp. 472-479 ◽  
Author(s):  
Timothy D. Heden ◽  
E. Matthew Morris ◽  
Monica L. Kearney ◽  
Tzu-Wen Liu ◽  
Young-min Park ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Protein Expression ◽  
Fatty Acid Profile ◽  
Fatty Acid Profiles ◽  
Sprague Dawley ◽  
High Fat ◽  
Low Fat ◽  
Fed State ◽  
Sprague Dawley Rats ◽  
Hepatic Triacylglycerol

The purpose of this study was to compare the effects of short-term low-fat (LF) and high-fat (HF) diets on fed-state hepatic triacylglycerol (TAG) secretion, the content of proteins involved in TAG assembly and secretion, fatty acid oxidation (FAO), and the fatty acid profile of stored TAG. Using selectively bred obese-prone Sprague–Dawley rats, we directly measured fed-state hepatic TAG secretion, using Tyloxapol (a lipoprotein lipase inhibitor) and a standardized oral mixed meal (45% carbohydrate, 40% fat, 15% protein) bolus in animals fed a HF or LF diet for 2 weeks, after which the rats were maintained on their respective diet for 1 week (washout) prior to the liver being excised to measure protein content, FAO, and TAG fatty acid profiles. Hepatic DGAT-1 protein expression was ∼27% lower in HF- than in LF-fed animals (p < 0.05); the protein expression of all other molecules was similar in the 2 diets. The fed-state hepatic TAG secretion rate was ∼39% lower (p < 0.05) in HF- (4.62 ± 0.18 mmol·h−1) than in LF- (7.60 ± 0.57 mmol·h−1) fed animals. Hepatic TAG content was ∼2-fold higher (p < 0.05) in HF- (1.07 ± 0.15 nmol·g−1tissue) than in LF- (0.50 ± 0.16 nmol·g−1tissue) fed animals. In addition, the fatty acid profile of liver TAG in HF-fed animals closely resembled the diet, whereas in LF-fed animals, the fatty acid profile consisted of mostly de novo synthesized fatty acids. FAO was not altered by diet. LF and HF diets differentially alter fed-state hepatic TAG secretion, hepatic fatty acid profiles, and DGAT-1 protein expression.

Rat lingual lipase: factors affecting enzyme activity and secretion.

1978 ◽  
Vol 235 (4) ◽  
pp. E416 ◽  
Author(s):  
M Hamosh
Keyword(s):  
Enzyme Activity ◽  
Subcellular Localization ◽  
Corn Oil ◽  
Secretory Granules ◽  
Fat Content ◽  
Control Group ◽  
Sprague Dawley ◽  
High Fat ◽  
Factors Affecting ◽  
Sprague Dawley Rats

The lingual serous glands of rat tongue secrete a potent lipase that acts in the stomach in which it initiates the digestion of dietary fat. The subcellular localization of the enzyme and factors affecting its activity and secretion were studied in adult male Sprague-Dawley rats. In a fraction rich in secretory granules, 42% of the lipase content of lingual serous glands was recovered after fractionation of homogenates of discontinuous gradients of urografin in 0.3 M sucrose. Lipase secretion was stimulated by isoprenaline: 2 h after isoprenaline administration, the lipase content of lingual serous glands was 73 +/- 5% lower than that of the control group. Accumulation of lipase began 8 h after the injection, reaching 57 +/- 7% of the initial level of the control group after 25 h. Bilateral resection of the glosso-pharyngeal nerves or bilateral sympathectomy led to a 40--50% decrease of enzyme activity in 7 days. Enzyme activity was markedly affected by the fat content of the diet. Feeding a high-fat diet (22% corn oil) for a period of 2 wk led to a 45% increase in the lipase content of lingual serous glands. The exponential rise in the lipase activity of the lingual serous glands immediately after birth could be related to the high-fat content of rat milk. The data suggest that the lingual serous glands are similar to other exocrine glands such as pancreas and parotid gland in the subcellular localization of secretory enzymes and mechanisms of enzyme secretion.

The Effects of Maternal High Fat Diet on the Lingual CD36 Lipid Sensors of the Offspring Sprague Dawley Rats

Metabolism ◽  
2021 ◽  
Vol 116 ◽  
pp. 154497
Author(s):  
Elif Günalan ◽  
Meyli Ezgi Karagöz ◽  
Bayram Yılmaz ◽  
Burcu Gemici
Keyword(s):  
High Fat Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Lipid Sensors

scholarly journals Short-Term High Fat Intake Does Not Significantly Alter Markers of Renal Function or Inflammation in Young Male Sprague-Dawley Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Catherine Crinigan ◽  
Matthew Calhoun ◽  
Karen L. Sweazea
Keyword(s):  
Kidney Disease ◽  
Renal Mass ◽  
Early Stage ◽  
Young Male ◽  
Protective Effects ◽  
Sprague Dawley ◽  
High Fat ◽  
Short Term ◽  
Sprague Dawley Rats ◽  
The Impact

Chronic high fat feeding is correlated with diabetes and kidney disease. However, the impact of short-term high fat diets (HFD) is not well-understood. Six weeks of HFD result in indices of metabolic syndrome (increased adiposity, hyperglycemia, hyperinsulinemia, hyperlipidemia, hyperleptinemia, and impaired endothelium-dependent vasodilation) compared to rats fed on standard chow. The hypothesis was that short-term HFD would induce early signs of renal disease. Young male Sprague-Dawley rats were fed either HFD (60% fat) or standard chow (5% fat) for six weeks. Morphology was determined by measuring changes in renal mass and microstructure. Kidney function was measured by analyzing urinary protein, creatinine, and hydrogen peroxide (H2O2) concentrations, as well as plasma cystatin C concentrations. Renal damage was measured through assessment of urinary oxDNA/RNA concentrations as well as renal lipid peroxidation, tumor necrosis factor alpha (TNFα), and interleukin 6 (IL-6). Despite HFD significantly increasing adiposity and renal mass, there was no evidence of early stage kidney disease as measured by changes in urinary and plasma biomarkers as well as histology. These findings suggest that moderate hyperglycemia and inflammation produced by short-term HFD are not sufficient to damage kidneys or that the ketogenic HFD may have protective effects within the kidneys.

Abstract 382: Anti-MAA Antibodies in Sprague Dawley Rats are Increased in Response to a High Fat Western Diet

2013 ◽  
Vol 33 (suppl_1) ◽  
Author(s):  
Michael J Duryee ◽  
Anand Dusad ◽  
Scott W Shurmur ◽  
Michael D Johnston ◽  
Robert P Garvin ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Normal Diet ◽  
Western Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Stable Plaque ◽  
Circulating Antibodies ◽  
Modified Proteins ◽  
Progression Of Atherosclerosis

Introduction Malondialdehyde/Acetaldehyde (MAA) modified proteins have been suggested to play a role in the development/progression of atherosclerosis. Circulating antibodies directed against these proteins have recently been shown to be associated with the severity of the disease. More specifically, the isotype of the antibody to MAA correlated with either an acute MI (IgG) or stable plaque formation (IgA) formation. MAA is thought to form as a result of the oxidation of fat(s) and thus the concentration and antibody response should reflect the amount of fat in the diet. Objective The purpose of this study was to evaluate the antibody responses to MAA modified proteins following immunization and high fat western diet feeding in rats. Methods Male Sprague Dawley rats were immunized with MAA-modified protein weekly for 5 weeks and then assayed for antibodies to these proteins. Animals were then separated into the following groups: chow sham, chow MAA immunized, high fat sham, and high fat MAA immunized. The high fat animals were fed a Western diet with 2-thiouracil for 12 weeks, bled every 3 weeks, and serum assayed for the presence of circulating MAA antibodies. Results Prior to feeding with high fat diet, rats immunized with MAA-modified protein had a significant increase (P<0.001) in serum antibodies directed against these modified proteins compared to controls (N of 4 per group). Following feeding of high fat diet antibody concentrations increased 6 fold in the high fat MAA immunized group compared to the chow MAA immunized group (P<0.05). Antibodies in the high fat sham and chow sham had only minimal increases in antibodies to these proteins. Conclusions These data demonstrate that following immunization with MAA-modified proteins, circulating antibodies are produced that increase following consumption of a high fat Western diet. It suggests that MAA-modified proteins are produced at low levels following normal diet, producing antibodies which act as a normal clearance method for altered protein. When high fat consumption increases these antibody levels are increased in response to the oxidative stress. Implications Use of these antibodies as a biomarker in the future may help predict the onset or progression of atherosclerosis.

Experimental obesity and osteoarthritis

1960 ◽  
Vol 198 (4) ◽  
pp. 765-770 ◽  
Author(s):  
Leon Sokoloff ◽  
Olaf Mickelsen ◽  
Emanuel Silverstein ◽  
George E. Jay ◽  
Richard S. Yamamoto
Keyword(s):  
Weight Gain ◽  
Dietary Restriction ◽  
High Fat Diet ◽  
Deleterious Effect ◽  
Degenerative Joint Disease ◽  
Fat Content ◽  
Joint Disease ◽  
High Fat ◽  
Low Fat ◽  
Hybrid Mice

Experimental obesity was produced in DBA/2JN, STR/N and C57L/HeN mice as well as in Osborne-Mendel rats by several dietary regimens. One of these, containing 60% vegetable fat, increased the amount of degenerative joint disease in the rats and in two strains of mice. No increase of osteoarthritis occurred as a result of a 37.4% fat content in the diet, or from obesity produced by Ingle's diet, which has a relatively low-fat content. The mechanism by which the high-fat diet increased the joint disease is unknown, because neither obesity nor a high-fat diet alone had a deleterious effect on the articulations of the mice. Obese hybrid mice derived from a spontaneously obese and arthritis-prone strain (STR/1N) were resistant to articular degeneration. Dietary restriction of weight gain in the STR/1N mice failed to decrease the osteoarthritis in them.

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