scholarly journals The effect of hydrolyzate on amino acid levels in Nile tilapia (Oreochromis niloticus)

Food Research ◽  
2021 ◽  
Vol 5 (S3) ◽  
pp. 85-89
Author(s):  
Nuryanto ◽  
E. Chasanah ◽  
D.N. Afifah ◽  
M. Sulchan ◽  
P. Martosuyono ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Protein Hydrolysate ◽  
Nutrient Content ◽  
Fish Protein ◽  
Protease Enzyme ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Fish Protein Hydrolysate

Fish is one of the most important foods in the human diet because of its high nutritional quality. One of the potential local foodstuffs in Grobogan is Nile tilapia (Oreochromis niloticus). Nile tilapia has a high nutrient content, especially protein. To obtain better benefits from fish protein, it can be processed into fish protein hydrolysate (FPH) products. Fish protein hydrolysates (FPH) which contain a mixture of small protein or peptides and free amino acids are beneficial for children diet, in preventing and combating malnutrition problems through readily absorbed essential amino acids. This study aimed to analyze the amino acid profile of Nile tilapia and fish protein hydrolysate (FPH) from Nile tilapia. The treatments included processing Nile tilapia into hydrolysate and analyse amino acids of Nile tilapia before and after hydrolysate. The process of making FPH uses a local microbial protease enzyme from Bacillus licheniformis. Amino acids composition was analysed using an internal method developed by Saraswanti Indo Genetech laboratory (18- 5-17/MU/SMM-SIG, UPLC). Data were analysed using the paired t-test statistical test. The results showed there were significant differences in the rate of all amino acid profiles (p<0.05), except L-Aspartic Acid, L-Tyrosine and L-Histidine (p>0.05). From the research, it can be concluded hydrolysate processing has an effect on the increase of Lglutamic acid and L-lysine amino acid.

scholarly journals PENGARUH PERBEDAAN SPESIES IKAN TERHADAP HIDROLISAT PROTEIN IKAN DENGAN PENAMBAHAN ENZIM PAPAIN (The Effect of Various Fish Species On Fish Protein Hydrolysate With The Addition of Papain Enzyme)

2017 ◽  
Vol 13 (1) ◽  
pp. 24 ◽  
Author(s):  
Strata Annisa ◽  
Yudhomenggolo Sastro Darmanto ◽  
Ulfah Amalia
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Fish Species ◽  
Research Method ◽  
Protein Hydrolysate ◽  
Amino Acid Profile ◽  
Fish Protein ◽  
Randomized Design ◽  
Fish Protein Hydrolysate ◽  
Completely Randomized Design

 Hidrolisat protein ikan (HPI) dihasilkan dari proses penguraian protein ikan menjadi peptida sederhana maupun asam amino melalui proses hidrolisis oleh enzim, asam, atau basa. Enzim yang digunakan adalah enzim protease, baik yang berasal dari tanaman, hewan maupun mikroba. Tujuan dari penelitian ini adalah mengetahui pengaruh penambahan enzim papain terhadap karakteristik HPI dan menentukan optimum terbaik hidrolisat protein dari jenis ikan yang berbeda dengan penambahan enzim papain. Metode penelitian ini adalah experimental laboratories dengan menggunakan Rancangan Acak Lengkap (RAL). Perlakuan yang diterapkan adalah perbedaan jenis ikan yang meliputi ikan nila, ikan bandeng, dan ikan cucut. Data yang diperoleh dianalisis dengan menggunakan sidik ragam ANOVA dengan uji lanjut untuk menentukan nilai yang berpengaruh maupun tidak dengan uji BNJ (Beda Nyata Jujur). Hasil penelitian dari ketiga perlakuan didapatkan HPI terbaik yaitu rendemen  HPI nila 5,64% dengan kadar protein 30,17%, kadar air 9,06%, dan viskositas 1,91 cP sedangkan profil asam amino terbanyak adalah asam glutamat terutama pada HPI ikan cucut sebesar 4,183%. Fish protein hydrolysate (FPH) is produced from the decomposition of fish protein become peptides and amino acids through hydrolysis by enzymes, acids or bases. The enzyme used are protease enzymes, both derived from plants, animals, and microbes. The purpose of this study was to determine the effect of papain enzyme addition on FPH characteristics and to determine the characteristics of the FPH and determine the best optimum of FPH from different fish species with the addition of the papain enzyme. This research method is experimental laboratories by using a Completely Randomized Design (CRD). The applied treatments applied are different fish species including tilapia, milkfish, and shark. The data obtained were analyzed by using ANOVA with further test to determine the value that influencd or not with BNJ test. The results of the research from the three treatments are yield FPH tilapia 5,64% with protein content 30,17%, water content 9,06%, and  viscosity 1,91 cP, while the highest amino acid profile is glutamic acid, especially in FPH shark 4,183%.  

Nutritional evaluation of fish protein hydrolysate and its application in piglet production

2021 ◽  
Author(s):  
Nan Zhang ◽  
Xiaoming Song ◽  
Wenxuan Dong ◽  
Ling Liu ◽  
Zhiying Cui ◽  
...  
Keyword(s):  
Amino Acids ◽  
Antioxidant Capacity ◽  
Protein Hydrolysate ◽  
Nutrient Digestibility ◽  
Adaptation Period ◽  
Fish Protein ◽  
Experimental Diet ◽  
Ileal Digestibility ◽  
Fish Protein Hydrolysate ◽  
Dietary Treatments

Abstract This study was conducted to determine the digestible energy (DE), metabolizable energy (ME), and ileal digestibility of amino acids (AA) of fish protein hydrolysate (FPH), and to evaluate the effects of FPH on the performance of piglets. In Exp. 1, the available energy content of FPH was determined by difference methods: 12 barrows with an initial body weight (BW) of 40.0±2.1kg were randomly assigned into two dietary treatments with six replicates per treatment, the treatments contained a corn basal diet and an experimental diet in which 20% corn was replaced by FPH. The experiment was lasted for 12 days, with 7 days adaptation period followed by 5 days total collection of feces and urine. In Exp. 2, the standardized ileal digestibility (SID) of AA in FPH by the N-free method was determined, 12 barrows (initial BW of 35.3±1.8 kg) with ileal T-cannulas were randomly allotted into two treatments with six replicates per treatment, the dietary treatments consisted of an experimental diet and a N-free diet. The experiment was lasted for 7 days, with 5 days adaptation period followed by 2 days collection of ileal digesta. In Exp. 3, the effect of FPH on the performance of piglets was conducted. 192 piglets (initial BW of 8.10±1.8kg) were randomly allotted into four treatments with six replicates per treatment. The treatments were a control diet with fish meal (FM) and part or all FM was replaced by FPH, got diets containing 2%, 3% or 5% FPH. The experiment lasted for 28 days. Results showed that: In Exp. 1, the DE and ME of FPH were 21.12MJ/kg and 20.28MJ/kg. In Exp. 2, the SID of Lys, Met, Thr and Trp were 79.99%, 87.17%, 68.29%, and 71.53% respectively. In Exp. 3, addition of 3% FPH increased nutrient digestibility and volatile fatty acid content in feces. Addition of 5% FPH increased the average daily feed intake (ADFI), significantly increased (P&lt;0.05) the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and immunoglobulin A (IgA), while significantly decreased (P&lt;0.05) the average daily gain (ADG). In conclusion, FPH had a high value of digestible energy and ileal digestible essential amino acids, could improve nutrient digestibility, immunity, antioxidant capacity, and intestinal health of piglets, while adverse to nitrogen deposition in piglets.

scholarly journals Establishing the optimal essential amino acid ratios in juveniles of Nile tilapia (Oreochromis niloticus) by the deletion method

2015 ◽  
Vol 22 (2) ◽  
pp. 435-443 ◽  
Author(s):  
A.F. Diógenes ◽  
J.B.K. Fernandes ◽  
J.C.P Dorigam ◽  
N.K. Sakomura ◽  
F.H.F. Rodrigues ◽  
...  
Keyword(s):  
Amino Acid ◽  
Oreochromis Niloticus ◽  
Essential Amino Acid ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Agricultural Bio-Stimulant Activity of Fish Protein Hydrolysate from Common Silver Belly (Gerres subfasciatus)

2021 ◽  
Vol 6 (5) ◽  
Author(s):  
Srinivasan S ◽  
◽  
Devika R ◽  
Shanmugam M ◽  
◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Hydrolysate ◽  
Germination Rate ◽  
Protein Hydrolysates ◽  
Green Gram ◽  
Lactobacillus Species ◽  
Fish Protein ◽  
Fish Protein Hydrolysate ◽  
Fish Proteins ◽  
Fish Protein Hydrolysates

Taking into account of using the fish industry wastes and for expanding the incentive to a few under used fish species, protein hydrolysates from fish proteins are being set up by a few specialists everywhere throughout the world. Fish Protein Hydrolysates (FPH) are breakdown results of enzymatic transformation of fish proteins into smaller units like peptides, which typically contain 2-20 amino acids. Their worth has been expanded by utilizing them as fluid bio compost, feed supplement and bioorganic fertilizer. From the research carried out at our laboratory, Dry fish silver belly with both microbe and enzyme acquired from cultural broth of lactobacillus species, catalyst papain demonstrated positive upgrade of development and advancement of plants tested. In view of the yield of FPH concentrates from both microbial and enzymatic hydrolysis, the pace of seedling life list in Urad dhal, green gram and okra got higher in 0.1-0.5% convergence of dry fish hydrolysate from lactobacillus treated than in the control variation. With respect to the seeds of urad dhal, green gram and okra, the germination rate and seedling energy list from treated seeds surpassed control by 19.11-128.71%. The positive effect of dry fish protein hydrolysate by microbial procedure on germination rate, seedling life record rate in 3 kinds of seeds is the reason for the improvement of protein hydrolysate item for application in crop creation utilizing dry fish squanders. The Seedling grown in 0.5% concentration of fish hydrolysates showed significant increase in seedling vigour index of Urad dhal (13.29 ± 0.12 %), Green gram (14.1 ± 0.17%) and Okra (5.39 ± 0.43 %) was recorded through one way Analysis of Variance (ANOVA). By HPLC determination amino acid results determined that protein hydrolysates process can be stopped on 7th day (643.56ppm) whereas @ 10th day amino acid decreased (326.24ppm). Results suggest that probiotic assumes a superior job in dry fish protein hydrolysates than compounds when contrasted and yield and prudent astute.

scholarly journals Characteristics of acid and pepsin solubilized collagens from Nile tilapia (Oreochromis niloticus) scale

2019 ◽  
pp. 95 ◽  
Author(s):  
Phanat Kittiphattanabawon ◽  
Chodsana Sriket ◽  
Hideki Kishimura ◽  
Soottawat Benjakul
Keyword(s):  
Amino Acid ◽  
Zeta Potential ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Ftir Spectra ◽  
Fish Scale ◽  
Alternative Source ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Sds Page ◽  
Tilapia Scale

In Thailand, the production of tilapia is about 140,000 tons, which is the sixth tilapia production in the world. From the processing, the scale was generated as waste. To produce a value-added product, the production of collagen from tilapia scale could be an alternative. Generally, collagen from fish scale possesses a less fishy odor and flavor than that from fish bone and skin. From the results, the fish scale is an interesting alternative source for collagen extraction. The objectives of this study were to extract collagen from Nile tilapia (Oreochromis niloticus) scale from the production of frozen tilapia fillet as well as to study its properties. Extraction of acid (ASC) and pepsin soluble collagens (PSC) from Nile tilapia scale, as well as the determination of their yield, amino acid compositions, SDS-PAGE patterns, FTIR spectra, thermal denaturation temperature (Tmax) and zeta potential, was conducted. ASC and PSC had a yield of 0.77 and 0.71% based on dry basis), respectively. The major amino acid found in both collagens was glycine (322-332 residues/1000 residues). Also, they had a high amount of imino acid (199-205 residues/1000 residues). Based on SDS-PAGE pattern, both collagens were classified as type I collagen ((α1)2α2-heterotrimer). The similar FTIR spectra of both collagens were found. Their amide peaks had no shift in wavenumber. ASC and PSC had Tmax of 36.15 and 34.70 °C, respectively. From zeta potential analysis, ASC and PSC had the zero of net chart at pH 5.09 and 5.84, respectively. Based on the characteristics of the collagen, the scale could be an alternative source for collagen production, however their yield should be improved to serve as a potential source for further application.

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