"Oxidative Stress Indices and Inflammatory Responses in Female Wistar Rats Administered Acetaminophen, Ampicillin/Cloxacillin and Co-Trimoxazole"

Background Ischemia–reperfusion (IR) injury is the main cause of delayed graft function in solid organ transplantation. Hypoxia-inducible factors (HIFs) control the expression of genes related to preconditioning against IR injury. During normoxia, HIF-α subunits are marked for degradation by the egg-laying defective nine homolog (EGLN) family of prolyl-4-hydroxylases. The inhibition of EGLN stabilizes HIFs and protects against IR injury. The aim of this study was to determine whether the EGLN inhibitors sodium (S)-2-hydroxyglutarate [(S)-2HG] and succinic acid (SA) have a nephroprotective effect against renal IR injury in Wistar rats. Methods (S)-2HG was synthesized in a 22.96% yield from commercially available L-glutamic acid in a two-step methodology (diazotization/alkaline hydrolysis), and its structure was confirmed by nuclear magnetic resonance and polarimetry. SA was acquired commercially. (S)-2HG and SA were independently evaluated in male and female Wistar rats respectively after renal IR injury. Rats were divided into the following groups: sham (SH), nontoxicity [(S)-2HG: 12.5 or 25 mg/kg; SA: 12.5, 25, or 50 mg/kg], IR, and compound+IR [(S)-2HG: 12.5 or 25 mg/kg; SA: 12.5, 25, or 50 mg/kg]; independent SH and IR groups were used for each assessed compound. Markers of kidney injury (BUN, creatinine, glucose, and uric acid) and liver function (ALT, AST, ALP, LDH, serum proteins, and albumin), proinflammatory cytokines (IL-1β, IL-6, and TNF-α), oxidative stress biomarkers (malondialdehyde and superoxide dismutase), and histological parameters (tubular necrosis, acidophilic casts, and vascular congestion) were assessed. Tissue HIF-1α was measured by ELISA and Western blot, and the expression of Hmox1 was assessed by RT-qPCR. Results (S)-2HG had a dose-dependent nephroprotective effect, as evidenced by a significant reduction in the changes in the BUN, creatinine, ALP, AST, and LDH levels compared with the IR group. Tissue HIF-1α was only increased in the IR group compared to SH; however, (S)-2HG caused a significant increase in the expression of Hmox1, suggesting an early accumulation of HIF-1α in the (S)-2HG-treated groups. There were no significant effects on the other biomarkers. SA did not show a nephroprotective effect; the only changes were a decrease in creatinine level at 12.5 mg/kg and increased IR injury at 50 mg/kg. There were no effects on the other biochemical, proinflammatory, or oxidative stress biomarkers. Conclusion None of the compounds were hepatotoxic at the tested doses. (S)-2HG showed a dose-dependent nephroprotective effect at the evaluated doses, which involved an increase in the expression of Hmox1, suggesting stabilization of HIF-1α. SA did not show a nephroprotective effect but tended to increase IR injury when given at high doses.

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Protective effects of glycyrrhizin on sub-chronic diazinon-induced biochemical, hematological alterations and oxidative stress indices in male Wistar rats

Drug and Chemical Toxicology ◽

10.1080/01480545.2018.1497053 ◽

2018 ◽

Vol 42 (3) ◽

pp. 300-308 ◽

Cited By ~ 7

Author(s):

Asieh Karimani ◽

Mohammad Heidarpour ◽

Amir Moghaddam Jafari

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Protective Effects ◽

Stress Indices ◽

Male Wistar Rats ◽

Hematological Alterations ◽

And Oxidative Stress

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Cyantraniliprole impairs reproductive parameters by inducing oxidative stress in adult female wistar rats

Reproductive Toxicology ◽

10.1016/j.reprotox.2021.12.009 ◽

2021 ◽

Author(s):

Suellen Ribeiro da Silva Scarton ◽

Felipe Tsuzuki ◽

Marina Trevizan Guerra ◽

Dayane Priscila dos Santos ◽

Aldair Casagrande dos Santos ◽

...

Keyword(s):

Oxidative Stress ◽

Adult Female ◽

Wistar Rats ◽

Reproductive Parameters ◽

Female Wistar Rats

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Effects of chia (Salvia hispanica L.) on oxidative stress and inflammation in ovariectomized adult female Wistar rats

Food & Function ◽

10.1039/c9fo00862d ◽

2019 ◽

Vol 10 (7) ◽

pp. 4036-4045 ◽

Cited By ~ 3

Author(s):

Bárbara Pereira da Silva ◽

Renata Celi Lopes Toledo ◽

Marcella Duarte Villas Mishima ◽

Maria Eliza de Castro Moreira ◽

Christiane Mileib Vasconcelos ◽

...

Keyword(s):

Oxidative Stress ◽

Adult Female ◽

High Fat Diet ◽

Wistar Rats ◽

Lipid Profiles ◽

Ovariectomized Rats ◽

High Fat ◽

Salvia Hispanica ◽

Female Wistar Rats ◽

Salvia Hispanica L

The study investigated the influence of chia consumption on inflammation, oxidative stress, and lipid profiles in female ovariectomized rats fed a high-fat diet.

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Comparisons of Curative Effects of Chlorophyll fromSauropus androgynus(L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

Journal of Toxicology ◽

10.1155/2016/8515089 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Suparmi Suparmi ◽

Minidian Fasitasari ◽

Martanto Martosupono ◽

Jubhar Christian Mangimbulude

Keyword(s):

Oxidative Stress ◽

Sodium Nitrate ◽

Wistar Rats ◽

Histological Evaluation ◽

Female Wistar Rats ◽

Male Wistar Rats ◽

Liver And Kidney ◽

Serum Biochemical ◽

Hb Concentration ◽

Rbc Count

Sodium nitrate (NaNO2) widely used as food additive for coloring and preserving meat has been reported to induce oxidative stress and cause histopathologic changes, nitrosative tissue damage, and lipid peroxidation in liver and kidney. Therefore, the present study compared the curative effect of chlorophyll fromSauropus androgynus(L) Merr and Cu-chlorophyllin as antioxidant in NaNO2-induced female Wistar rats based on haematological, serum biochemical, and histological evaluation. Thirty male Wistar rats were randomly assigned into six groups of five rats each. NaNO2were given at a subacute dose of 50 mg/kg bw intraperitoneally for 10 days. Chlorophyll fromS. androgynusand Cu-chlorophyllin from K-Liquid™ were given in the following 14 days at the two doses: 0,016 mg/mL and 0.008 mg/mL. NaNO2exposure resulted in significant reductions (p<0.05) in values of packed cell volume (PCV), haemoglobin (Hb) concentration and red blood cell (RBC) count, transferrin, and ferritin and elevation in malondialdehyde (MDA) level and schistocytes percentage with insignificant reductions in serum albumin and transferrin levels. Histology of kidney and liver were changed insignificantly (p>0.05) to normal values. Chlorophyll fromS. androgynusand Cu-chlorophyllin possess antioxidant potentials to protect against toxicities induced by sodium nitrate.

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Pomegranate Seed Extract: A Strong Antioxidant against Benign Prostatic Hyperplasia Induced Oxidative Stress in Albino Wistar Rats

Journal of Cancer and Tumor International ◽

10.9734/jcti/2021/v11i430160 ◽

2021 ◽

pp. 50-60

Author(s):

U. A. Obisike ◽

N. Boisa ◽

E. O. Nwachuku

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Total Antioxidant Status ◽

Punica Granatum ◽

Stress Indices ◽

Ethanolic Extracts ◽

Total Antioxidant ◽

Pomegranate Seed ◽

Oxidant Status ◽

Benign Prostate

Aim: The aim of this study was to assess the antioxidant properties of ethanolic and aqueous extracts of Punica granatum (Pomegranate) seed in testosterone induced benign prostate hyperplastic albino Wistar rats. Study design: This study is an interventional study. Place and Duration of Study: The experimental aspect of this study was conducted at the animal house, Department of Pharmacology, University of Port Harcourt between April and September, 2019. Methodology: Seventy (70) adult albino male wistar rats were used for this study. They were divided into 12 groups of 5 rats each and fed with commercial rat diet and clean drinking water. Aqueous and ethanolic extracts of Punica granatum seed were prepared using the maceration method. Benign Prostate Hyperplasia was induced in rats after they submitted to bilateral orchiectomy by daily injections of testosterone propionate (TP) (4 mg/kg b.wt.sc). Rats were treated with 500 or 1500 mg/kg b.wt. of aqueous or ethanoI extracts of Punica granatum seed, dutasteride or in combination. Administration of extracts was done by gavage. Plasma total oxidant status (TOS), total antioxidant status (TAS), superoxide dismutase (SOD) activity, were analyzed using sandwich ELISA Kits by Shanghai Korain Biotech Co., Ltd, China, while oxidative stress indices (OSI) were calculated. Statistical analysis was done using SPSS version 22.0 of Windows Stat Pac and p values less than 0.05 were considered statistically significant. Results: The results showed that the mean TOS, TAS, SOD and OSI for the rats in the normal control group were 1.66±0.2U/ml, 2.71±0.25U/ml, 41.8±2.9pg/ml and 0.62±0.10 respectively. After BPH induction (group 2), the values were 3.25±0.5U/ml,1.17±0.14U/ml, 23.38±2.09 pg/ml and 2.81±0.60pg/ml respectively. There were significant decreases for TOS and OSI, and significant increases for TAS and SOD when the rats where treated with lower and higher doses of both aqueous and ethanolic extracts of Punica granatum and Dutasteride. Conclusion: In conclusion, both doses of Punica granatum seed for ethanolic and aqueous extracts individually and in combination and with dutasteride markedly reduced total oxidant status, oxidative stress indices and improved the activities of antioxidant parameters like superoxide dismutase and total antioxidant status.

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Methyl Gallate Attenuates Doxorubicin-Induced Cardiotoxicity in Rats by Suppressing Oxidative Stress

Scientifica ◽

10.1155/2021/6694340 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Akheruz Zaman Ahmed ◽

Shakta Mani Satyam ◽

Prakashchandra Shetty ◽

Melanie Rose D’Souza

Keyword(s):

Oxidative Stress ◽

Normal Control ◽

Wistar Rats ◽

Group Iv ◽

Control Group ◽

Methyl Gallate ◽

Group Iii ◽

Group I ◽

Female Wistar Rats ◽

Group Ii

Doxorubicin-induced cardiotoxicity is the leading cause of morbidity and mortality among cancer survivors. The present study was aimed to investigate the cardioprotective potential of methyl gallate; an active polyphenolic nutraceutical, against doxorubicin-induced cardiotoxicity in Wistar rats. Twenty-four female Wistar rats (150–200 g) were divided into four groups (n = 6) which consist of normal control (group I), doxorubicin control (group II), test-A (group III), and test-B (group IV). Group III and group IV animals were prophylactically treated with methyl gallate 150 mg/kg/day and 300 mg/kg/day orally, respectively, for seven days. Doxorubicin (25 mg/kg; single dose) was administered through an intraperitoneal route to group II, III, and IV animals on the seventh day to induce acute cardiotoxicity. On the 8th day, besides ECG analysis, serum CK, CK-MB, LDH, AST, MDA, and GSH were assayed. Following gross examination of isolated hearts, histopathological evaluation was performed by light microscopy. A significant ( p < 0.05) cardiac injury, as well as oxidative stress, was observed in doxorubicin control rats in comparison to normal control rats. Methyl gallate at both the doses significantly ( p < 0.05) reduced doxorubicin-induced ECG changes, dyslipidaemia, and elevation of CK, CK-MB, LDH, AST, MDA and increased GSH level. Methyl gallate reversed the doxorubicin-induced histopathological changes in the heart. The present study revealed that methyl gallate exerts cardioprotection against doxorubicin-induced cardiotoxicity in female Wistar rats by suppressing oxidative stress. Our study opens the perspective to clinical studies for consideration of methyl gallate as a potential chemoprotectant nutraceutical in the combination chemotherapy with doxorubicin to limit its cardiotoxicity.

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