scholarly journals Spectrophotometric flow injection procedure to indirect determination of paracetamol in pharmaceutical formulations using o-tolidine as reagent

2018 ◽  
Vol 33 (2) ◽  
pp. 47
Author(s):  
Orlando Fatibello-Filho ◽  
Heberth Juliano Vieira
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Flow Injection ◽  
Pharmaceutical Formulations ◽  
Injection Method ◽  
Indirect Determination ◽  
Analytical Curve ◽  
Relative Standard ◽  
Injection Procedure

A spectrophotometric flow injection method for the determination of paracetamol in pharmaceutical formulations is proposed. The procedure was based on the oxidation of paracetamol by sodium hypochloride and the determination of the excess of this oxidant using o-tolidine dichloride as chromogenic reagent at 430 nm. The analytical curve was linear in the paracetamol concentration range from 8.50 x 10-6 to 2.51 x 10-4 mol L-1 with a detection limit of 5.0 x 10-6 mol L-1. The relative standard deviation was smaller than 1.2% for 1.20 x 10-4 mol L-1 paracetamol solution (n = 10). The results obtained for paracetamol in pharmaceutical formulations using the proposed flow injection method and those obtained using a USP Pharmacopoeia method are in agreement at the 95% confidence level.

scholarly journals Spectrophotometric flow injection procedure to indirect determination of paracetamol in pharmaceutical formulations using o-tolidine as reagent

2008 ◽  
Vol 33 (2) ◽  
pp. 47-54 ◽  
Author(s):  
O. Fatibello-Filho ◽  
H. J. Vieira
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Flow Injection ◽  
Pharmaceutical Formulations ◽  
Injection Method ◽  
Indirect Determination ◽  
Analytical Curve ◽  
Relative Standard ◽  
Injection Procedure

A spectrophotometric flow injection method for the determination of paracetamol in pharmaceutical formulations is proposed. The procedure was based on the oxidation of paracetamol by sodium hypochloride and the determination of the excess of this oxidant using o-tolidine dichloride as chromogenic reagent at 430 nm. The analytical curve was linear in the paracetamol concentration range from 8.50 x 10-6 to 2.51 x 10-4 mol L-1 with a detection limit of 5.0 x 10-6 mol L-1. The relative standard deviation was smaller than 1.2% for 1.20 x 10-4 mol L-1 paracetamol solution (n = 10). The results obtained for paracetamol in pharmaceutical formulations using the proposed flow injection method and those obtained using a USP Pharmacopoeia method are in agreement at the 95% confidence level.

scholarly journals Ultrasensitive determination of amoxicillin using chemiluminescence with flow injection analysis

2006 ◽  
Vol 20 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Xiaofeng Xie ◽  
Zhenghua Song
Keyword(s):  
Hydrogen Peroxide ◽  
Standard Deviation ◽  
Detection Limit ◽  
Flow Injection ◽  
Pharmaceutical Preparations ◽  
Chemiluminescence Intensity ◽  
Relative Standard ◽  
Human Urine And Serum ◽  
Urine And Serum

Results presented here reveal that amoxicillin can greatly enhance the chemiluminescence intensity generated from the reaction between luminol and hydrogen peroxide. The increment chemiluminescence signal was linearly dependent on amoxicillin concentration in the range from 10 pg·ml−1to 2 ng·ml−1(r2=0.9978) offering a detection limit as low as 3.5 pg·ml−1(3σ). At a flow rate of 2.0 ml·min−1, one analysis cycle, including sampling and washing, can be accomplished in 20 s with a relative standard deviation of less than 5%. The sensitive flow injection method was applied successfully to determine of amoxicillin in pharmaceutical preparations, human urine and serum without any pretreatment procedure, with recovery from 90.0% to 110.0% and relative standard deviations of less than 5.0%.

scholarly journals Flow-injection spectrophotometric determination of captopril in pharmaceutical formulations using a new solid-phase reactor containing AgSCN immobilized in a polyurethane resin

2012 ◽  
Vol 48 (2) ◽  
pp. 325-333 ◽  
Author(s):  
Fernando Campanhã Vicentini ◽  
Willian Toito Suarez ◽  
Éder Tadeu Gomes Cavalheiro ◽  
Orlando Fatibello-Filho
Keyword(s):  
Flow Injection ◽  
Solid Phase ◽  
Pharmaceutical Formulations ◽  
Analysis Procedure ◽  
Polyurethane Resin ◽  
Analytical Curve ◽  
Relative Standard ◽  
Solid Phase Reactor ◽  
Simple Flow

A simple flow-injection analysis procedure was developed for determining captopril in pharmaceutical formulations employing a novel solid-phase reactor containing silver thiocyanate immobilized in a castor oil derivative polyurethane resin. The method was based on silver mercaptide formation between the captopril and Ag(I) in the solid-phase reactor. During such a reaction, the SCN- anion was released and reacted with Fe3+, which generated the FeSCN2+ complex that was continuously monitored at 480 nm. The analytical curve was linear in the captopril concentration range from 3.0 × 10-4 mol L-1 to 1.1 × 10-3 mol L-1 with a detection limit of 8.0 × 10-5 mol L-1. Recoveries between 97.5% and 103% and a relative standard deviation of 2% for a solution containing 6.0 × 10-4 mol L-1 captopril (n = 12) were obtained. The sample throughput was 40 h-1 and the results obtained for captopril in pharmaceutical formulations using this procedure and those obtained using a pharmacopoeia procedure were in agreement at a 95% confidence level.

scholarly journals Determination of Manganese in Feedstuffs by Flow Injection Spectrophotometry Based on Rapid Formation of Permanganate at Room Temperature

2001 ◽  
Vol 84 (4) ◽  
pp. 1011-1016 ◽  
Author(s):  
Jing-Fu Liu ◽  
Ying-Di Feng ◽  
Gui-Bin Jiang
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Flow Injection ◽  
Room Temperature ◽  
Sampling Rate ◽  
Relative Standard ◽  
Rapid Flow ◽  
Rapid Formation ◽  
Optimized Conditions

Abstract A simple and rapid flow injection spectrophotometric procedure was developed for determination of manganese. In the presence of pyrophosphate and acetate, manganese was immediately oxidized to permanganate by periodate at room temperature in slightly alkaline medium. Under optimized conditions, the determination was made with a sampling rate of 120/h, a linear range of 0–30 mg/L Mn(II), a detection limit (S/N = 3) of 0.08 mg/L, and a relative standard deviation of 0.6% (n = 11) at 10 mg/L Mn(II). The proposed method was used to determine manganese in trace mineral premixes and feedstuffs. Results agreed well with those obtained by the standard atomic absorption spectroscopy method.

Determination of Prednisone Acetate at Trace Level by Flow Injection Analysis with Tris(1,10-Phenanthroline) Ruthenium(II) Chemiluminescence Detection

2011 ◽  
Vol 236-238 ◽  
pp. 2729-2732
Author(s):  
Pi Xue Gong ◽  
Wei Hua Liu ◽  
Wen Wen Liu ◽  
Kang Du ◽  
Kang Liu ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Flow Injection ◽  
Acidic Medium ◽  
Luminescent Properties ◽  
Chemiluminescence Detection ◽  
Trace Level ◽  
Experimental Conditions ◽  
Relative Standard

A simple, sensitive and rapid method for flow injection with chemiluminescence analysis has been developed for the determination of prednisone acetate at trace level. This method is based on the luminescent properties of the tris(1,10-phenanthroline) ruthenium(II)-potassium permanganate -prednisone acetate in acidic medium sensitized by Na2SO3. The optimized experimental conditions are evaluated. Under the optimum experimental conditions, the linear ranges of prednisone acetate are: 4.0×10-10~4.0×10-8g·mL-1and 4.0×10-8~2.0×10-7g·mL-1with a low detection limit is 4.5×10-11g·mL-1and the relative standard deviation for 1.0×10-8g·mL-1sample is 1.04% (n=11). The proposed method has been successfully applied to the determination of prednisone acetate in tablets.

scholarly journals Determinação de rutina em formulações farmacêuticas utilizando um sistema de análise por injeção em fluxo envolvendo multicomutação

2017 ◽  
Vol 41 (1) ◽  
pp. 32
Author(s):  
Déborah C. Azzi ◽  
Geiser G. Oliveira ◽  
Bruno C. Janegitz ◽  
Viviane G. Bonifácio ◽  
Orlando Fatibello-Filho ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Pharmaceutical Formulations ◽  
Sampling Frequency ◽  
Pharmaceutical Products ◽  
Chemiluminescence Method ◽  
Analytical Curve ◽  
Relative Standard ◽  
Flow Injection Chemiluminescence ◽  
Optimized Conditions

This paper reports a flow injection chemiluminescence method involving multicommutation for rutin determination in pharmaceutical formulations. The method is based on the oxidation of rutin by sodium hypochlorite which decreases the signal magnitude when compared with the same reaction without the rutin. Under optimized conditions, an analytical curve was obtained for rutin concentration range from 5.0 × 10-6 mol L-1 to 1.0 × 10-4 mol L-1 (r = 0.9966) with detection limit of 9.7 × 10-7 mol L-1 and a sampling frequency of 120 h-1. Repeatability study for a 7.0 × 10-6 mol L-1 rutine concentration showed a relative standard deviation (RSD) less than 5.0% for ten consecutives determination. The proposed method was applied successfully for rutin determination in pharmaceutical products.

scholarly journals Spectrophotometric determination of nicradipine and isradipine in pharmaceutical formulations

2009 ◽  
Vol 15 (2) ◽  
pp. 69-76 ◽  
Author(s):  
S.M. Al-Ghannam ◽  
A.M. Al-Olyan
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Reaction Pathway ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Zinc Powder ◽  
Conditional Stability ◽  
Conditional Stability Constant

A sensitive spectrophotometric method was developed for the determination of some 1,4-dihydropyridine compounds namely, nicardipine and isradipine either in pure form or in pharmaceutical preparations. The method is based on the reduction of nicardipine and isradipine with zinc powder and calcium chloride followed by further reduction with sodium pentacyanoaminoferrate (II) to give violet and red products having the absorbance maximum at 546 and 539 nm with nicardipine and isradipine, respectively. Beer's law was obeyed over the concentration range 8.0-180 ?g/ml with the detection limit of 1.67 ?g/ml for nicardipine and 8.0-110 ?g/ml with the detection limit of 1.748 ?g/ml for isradipine. The analytical parameters and their effects on the reported methods were investigated. The molar absorptivity, quantization limit, standard deviation of intercept (Sa), standard deviation of slope (Sb) and standard deviation of the residuals (Sy/x) were calculated. The composition of the result compounds were found 1:1 for nicardipine and 1:2 for isradipine by Job's method and the conditional stability constant (Kf) and the free energy changes (?G) were calculated for compounds formed. The proposed method was applied successfully for the determination of nicardipine and isradipine in their dosage forms. The results obtained were in good agreement with those obtained using the reference or official methods. A proposal of the reaction pathway was presented.

scholarly journals A sensitive spectrophotometric determination of tadalafil in pharmaceutical preparations and industrial wastewater samples

2013 ◽  
Vol 10 (3) ◽  
pp. 1005-1013 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Standard Deviation ◽  
Industrial Wastewater ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Average Recovery ◽  
Relative Standard ◽  
Wastewater Samples ◽  
Sensitive Spectrophotometric Method

A simple, accurate, precise, rapid, economical and a high sensitive spectrophotometric method has been developed for the determination of tadalafil in pharmaceutical preparations and industrial wastewater samples, which shows a maximum absorbance at 204 nm in 1:1 ethanol-water. Beer's law was obeyed in the range of 1-7?g/ mL ,with molar absorptivity and Sandell ? s sensitivity of 0.783x105l/mol.cm and 4.97 ng/cm2respectively, relative standard deviation of the method was less than 1.7%, and accuracy (average recovery %) was 100 ± 0. 13. The limits of detection and quantitation are 0.18 and 0.54 µg .ml-1, respectively. The method was successfully applied to the determination of tadalafil in some pharmaceutical formulations (tablets) and industrial wastewater samples. The proposed method was validated by sensitivity and precision which proves suitability for the routine analysis of tadalafil in true samples.

scholarly journals Determination of Sulfate in Natural and Residual Waters by Turbidimetric Flow-Injection Analysis

2001 ◽  
Vol 84 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Inês P A Morais ◽  
António O S S Rangel ◽  
M Renata S Souto
Keyword(s):  
Detection Limit ◽  
Flow Injection ◽  
Reference Method ◽  
Previous Treatment ◽  
Barium Chloride ◽  
Sulfate Solution ◽  
Injection System ◽  
Residual Water ◽  
Relative Standard

Abstract A turbidimetric flow-injection system was developed for the determination of sulfate in natural and residual water samples, with no previous treatment, using spectrophotometric detection. The precipitating agent, 7.0% (w/v) barium chloride solution prepared in 0.10% (w/v) polyvinyl alcohol, was added by using the merging-zones approach. A 100 mg/L sulfate solution in 0.07M nitric acid was mixed with the sample before it entered the injection loop to improve the detection limit, provide in-line pH adjustment, and prevent the interference of some anionic species. The relative standard deviations of the results were between 1.4 and 3.0% and were in agreement with results obtained by the reference method. Samples within a linear concentration range of 10–120 mg SO42−/L can be analyzed at a rate of 40/h. The detection limit is 5 mg SO42−/L.

Automated multiple flow-injection analysis in clinical chemistry: determination of albumin with bromcresol green.

1980 ◽  
Vol 26 (2) ◽  
pp. 331-334 ◽  
Author(s):  
B W Renoe ◽  
K K Stewart ◽  
G R Beecher ◽  
M R Wills ◽  
J Savory
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Clinical Chemistry ◽  
Analytical Results ◽  
Relative Standard ◽  
Clinical Chemical ◽  
Bromcresol Green

Abstract We describe an adaptation of automated multiple flow-injection analysis instrumentation to an analysis for albumin in serum. The bromcresol green reaction was used to test the utility of the system. The approach yielded albumin results with excellent sensitivity, no measurable carryover, a relative standard deviation of less than 1%, good correlations with published procedures, and no measurable interferences. The simplicity and flexibility of the instrumentation and its performance integrity, as indicated by the analytical results, make this a viable clinical chemical tool.

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