In vitro cartilage differentiation of human adipose-derived mesenchymal stem cell spheroids cultured in porous scaffolds

ADSC spheroids derived on various biomaterials present different in vitro properties, which may explain their different efficacies in cartilage repair.

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In vitro osteogenic differentiation of adipose-derived mesenchymal stem cell spheroids impairs their in vivo vascularization capacity inside implanted porous polyurethane scaffolds

Acta Biomaterialia ◽

10.1016/j.actbio.2014.06.035 ◽

2014 ◽

Vol 10 (10) ◽

pp. 4226-4235 ◽

Cited By ~ 33

Author(s):

Matthias W. Laschke ◽

Timo E. Schank ◽

Claudia Scheuer ◽

Sascha Kleer ◽

Takhirjan Shadmanov ◽

...

Keyword(s):

Stem Cell ◽

Mesenchymal Stem Cell ◽

Osteogenic Differentiation ◽

Cell Spheroids

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Faculty Opinions recommendation of MicroRNA-100 shuttled by mesenchymal stem cell-derived exosomes suppresses in vitro angiogenesis through modulating the mTOR/HIF-1α/VEGF signaling axis in breast cancer cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.727851155.793537268 ◽

2017 ◽

Author(s):

Heather Francis

Keyword(s):

Breast Cancer ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Vegf Signaling ◽

Signaling Axis ◽

In Vitro Angiogenesis

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Delivery of miR-381-3p Mimic by Mesenchymal Stem Cell-Derived Exosomes Inhibits Triple Negative Breast Cancer Aggressiveness; an In Vitro Study

Stem Cell Reviews and Reports ◽

10.1007/s12015-020-10089-4 ◽

2021 ◽

Author(s):

Samaneh Shojaei ◽

Seyed Mahmoud Hashemi ◽

Hossein Ghanbarian ◽

Kazem Sharifi ◽

Mohammad Salehi ◽

...

Keyword(s):

Breast Cancer ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Triple Negative Breast Cancer ◽

Triple Negative ◽

In Vitro Study ◽

Vitro Study ◽

Cancer Aggressiveness

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Uptake and Distribution of Administered Bone Marrow Mesenchymal Stem Cell Extracellular Vesicles in Retina

Cells ◽

10.3390/cells10040730 ◽

2021 ◽

Vol 10 (4) ◽

pp. 730

Author(s):

Biji Mathew ◽

Leianne A. Torres ◽

Lorea Gamboa Gamboa Acha ◽

Sophie Tran ◽

Alice Liu ◽

...

Keyword(s):

Stem Cell ◽

Mesenchymal Stem Cell ◽

Extracellular Vesicles ◽

Time Course ◽

Ganglion Cells ◽

Ex Vivo ◽

Dependent Manner ◽

Paracrine Effects

Cell replacement therapy using mesenchymal (MSC) and other stem cells has been evaluated for diabetic retinopathy and glaucoma. This approach has significant limitations, including few cells integrated, aberrant growth, and surgical complications. Mesenchymal Stem Cell Exosomes/Extracellular Vesicles (MSC EVs), which include exosomes and microvesicles, are an emerging alternative, promoting immunomodulation, repair, and regeneration by mediating MSC’s paracrine effects. For the clinical translation of EV therapy, it is important to determine the cellular destination and time course of EV uptake in the retina following administration. Here, we tested the cellular fate of EVs using in vivo rat retinas, ex vivo retinal explant, and primary retinal cells. Intravitreally administered fluorescent EVs were rapidly cleared from the vitreous. Retinal ganglion cells (RGCs) had maximal EV fluorescence at 14 days post administration, and microglia at 7 days. Both in vivo and in the explant model, most EVs were no deeper than the inner nuclear layer. Retinal astrocytes, microglia, and mixed neurons in vitro endocytosed EVs in a dose-dependent manner. Thus, our results indicate that intravitreal EVs are suited for the treatment of retinal diseases affecting the inner retina. Modification of the EV surface should be considered for maintaining EVs in the vitreous for prolonged delivery.

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Helicobacter pylori Infection of Gastrointestinal Epithelial Cells in vitro Induces Mesenchymal Stem Cell Migration through an NF-κB-Dependent Pathway

PLoS ONE ◽

10.1371/journal.pone.0029007 ◽

2011 ◽

Vol 6 (12) ◽

pp. e29007 ◽

Cited By ~ 32

Author(s):

Jonathan Ferrand ◽

Philippe Lehours ◽

Annie Schmid-Alliana ◽

Francis Mégraud ◽

Christine Varon

Keyword(s):

Helicobacter Pylori ◽

Stem Cell ◽

Cell Migration ◽

Epithelial Cells ◽

Mesenchymal Stem Cell ◽

Helicobacter Pylori Infection ◽

Stem Cell Migration ◽

Dependent Pathway

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Biomaterials Nano Geometry for Control of Stem Cell Differentiation

MRS Proceedings ◽

10.1557/proc-1239-vv02-02 ◽

2009 ◽

Vol 1239 ◽

Author(s):

Karla Brammer ◽

Seunghan Oh ◽

Sungho Jin

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Stem Cell Differentiation ◽

Human Mesenchymal Stem Cell ◽

Oxide Surface ◽

Specific Cell ◽

Implant Surface ◽

Multipotent Stem Cells

AbstractTwo important goals in stem cell research are to control the cell proliferation without differentiation, and also to direct the differentiation into a specific cell lineage when desired. Recent studies indicate that the nanostructures substantially influence the stem cell behavior. It is well known that mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into stromal lineages such as adipocyte, chondrocyte, fibroblast, myocyte, and osteoblast cell types. By examining the cellular behavior of MSCs cultured in vitro on nanostructures, some understanding of the effects that the nanostructures have on the stem cell’s response has been obtained. Here we demonstrate that TiO2 nanotubes produced by anodization on Ti implant surface can regulate human mesenchymal stem cell (hMSC) differentiation towards an osteoblast lineage in the absence of osteogenic inducing factors. Altering the dimensions of nanotubular-shaped titanium oxide surface structures independently allowed either augmented human mesenchymal stem cell (hMSC) adhesion at smaller diameter levels or a specific differentiation of hMSCs into osteoblasts using only the geometric cues. Small (˜30 nm diameter) nanotubes promoted adhesion without noticeable differentiation, while larger (˜70 - 100 nm diameter) nanotubes elicited a dramatic, ˜10 fold stem cell elongation, which induced cytoskeletal stress and selective differentiation into osteoblast-like cells, offering a promising nanotechnology-based route for novel orthopaedics-related hMSC treatments. The fact that a guided and preferential osteogenic differentiation of stem cells can be achieved using substrate nanotopography alone without using potentially toxic, differentiation-inducing chemical agents is significant, which can be useful for future development of novel and enhanced stem cell control and therapeutic implant development.

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Size-controlled human adipose-derived stem cell spheroids hybridized with single-segmented nanofibers and their effect on viability and stem cell differentiation

Biomaterials Research ◽

10.1186/s40824-021-00215-9 ◽

2021 ◽

Vol 25 (1) ◽

Author(s):

Jinkyu Lee ◽

Sangmin Lee ◽

Sung Min Kim ◽

Heungsoo Shin

Keyword(s):

Stem Cell ◽

Cell Function ◽

Average Length ◽

Three Dimensional ◽

Stem Cell Differentiation ◽

Cell Spheroids ◽

Significant Difference ◽

Vitro Analysis ◽

Physical And Chemical

Abstract Background Fabrication of three-dimensional stem cell spheroids have been studied to improve stem cell function, but the hypoxic core and limited penetration of nutrients and signaling cues to the interior of the spheroid were challenges. The incorporation of polymers such as silica and gelatin in spheroids resulted in relatively relaxed assembly of composite spheroids, and enhancing transport of nutrient and biological gas. However, because of the low surface area between cells and since the polymers were heterogeneously distributed throughout the spheroid, these polymers cannot increase the cell to extracellular matrix interactions needed to support differentiation. Methods We developed the stem cell spheroids that incorporate poly(ι-lactic acid) single-segmented fibers synthesized by electrospinning and physical and chemical fragmentation. The proper mixing ratio was 2000 cells/μg fibers (average length of the fibers was 50 μm - 100 μm). The SFs were coated with polydopamine to increase cell binding affinity and to synthesize various-sized spheroids. The function of spheroids was investigated by in vitro analysis depending on their sizes. For statistical analysis, Graphpad Prism 5 software (San Diego, CA, USA) was used to perform one-way analysis of variance ANOVA with Tukey’s honest significant difference test and a Student’s t-test (for two variables) (P < 0.05). Results Spheroids of different sizes were created by modulating the amount of cells and fibers (0.063 mm2–0.322 mm2). The fibers in the spheroid were homogenously distributed and increased cell viability, while cell-only spheroids showed a loss of DNA contents, internal degradation, and many apoptotic signals. Furthermore, we investigated stemness and various functions of various-sized fiber-incorporated spheroids. In conclusion, the spheroid with the largest size showed the greatest release of angiogenic factors (released VEGF: 0.111 ± 0.004 pg/ng DNA), while the smallest size showed greater effects of osteogenic differentiation (mineralized calcium: 18.099 ± 0.271 ng/ng DNA). Conclusion The spheroids incorporating polydopamine coated single-segmented fibers showed enhanced viability regardless of sizes and increased their functionality by regulating the size of spheroids which may be used for various tissue reconstruction and therapeutic applications.

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