scholarly journals Serum thromboxane B2 (TXB2) Determination is Influenced by Sample Incubation Temperature in Healthy Beagle Dogs

2009 ◽  
Vol 78 (2) ◽  
pp. 223-228 ◽  
Author(s):  
Aleš Jerin ◽  
Alenka Seliškar ◽  
Barbara Lukanc ◽  
Janoš Butinar ◽  
Alenka Nemec Svete
Keyword(s):  
Room Temperature ◽  
Incubation Temperature ◽  
Beagle Dogs ◽  
Specific Test ◽  
Blood Samples ◽  
Inflammatory Drugs ◽  
The Mean ◽  
Serum Thromboxane ◽  
Mean Concentration ◽  
Cox 1

The measurement of serum thromboxane B2 (TXB2) production by platelets is a specific test for assessment of platelet cyclooxygenase (COX-1) activity following administration of non-steroidal anti-inflammatory drugs (NSAIDs). The aim of this study was to investigate the influence of sample incubation at 37 °C for one hour on serum TXB2 concentration in comparison with incubation at room temperature. A total of 54 blood samples for serum TXB2 measurements were collected from six healthy beagle dogs into two separate serum tubes. While one group of tubes was incubated in a 37 °C water bath, the second group of tubes was left to coagulate at room temperature, both for one hour. Serum TXB2 concentrations were measured by ELISA. The mean concentration (± SD) of serum TXB2 in the group of samples that were incubated at 37 °C was significantly (P < 0.0001) higher compared to the group of samples incubated at room temperature, 1098 ± 346 μg/l and 550 ± 257 μg/l, respectively. The results of the study provide the information on serum TXB2 concentration in healthy beagle dogs and demonstrate that validated methods for assessment of COX-1 activity by measurement of serum TXB2 should be used in order to make results more reliable and comparable between different studies. The results of this study might be of great help in planning NSAID studies in dogs by providing the information that TXB2 generation by platelets is influenced profoundly by incubation temperature.

scholarly journals Can NLR, PLR and LMR be used as prognostic indicators in patients with pulmonary embolism? Author’s reply on commentary

2020 ◽  
Author(s):  
Nuri Kose ◽  
Tarık Yıldırım ◽  
Fatih Akın ◽  
Seda Elçim Yıldırım ◽  
Ibrahim Altun
Keyword(s):  
Inflammatory Diseases ◽  
Emergency Admission ◽  
Prognostic Indicators ◽  
Measuring Time ◽  
Duration Of Symptoms ◽  
Blood Samples ◽  
Inflammatory Parameters ◽  
Inflammatory Drugs ◽  
The Mean ◽  
Collagen Tissue

We appreciate the comments made by Dr Bedel and colleagues. NLR, PLR and LMR are affected by various diseases such as oncological, collagen tissue, inflammatory, or severe renal/liver diseases [1]. Because of this, we have listed some of the above-mentioned disorders in the tables. Hematological diseases, collagen tissue disease, inflammatory diseases, congenital heart disease, or severe renal/liver disease were therefore excluded from the study. However, the presence of malignancy did not affect our results in regression analysis.Platelets swell until 120 minutes in ethylene diamine tetra acetic (EDTA) and until 60 minutes in citrate [2]. Authors suggest that optimal measuring time should not exceed 120 minutes. The blood samples of the patients were taken within 1 hour after their emergency admission. All blood samples in our study were tested within 1 hour of collection [3]. We used EDTA for whole blood anticoagulation. The mean duration of symptoms prior to admission was 5.04 ± 6.9 days. The drugs such as corticosteroids affect inflammatory parameters. Therefore, we excluded inflammatory diseases without emphasizing corticosteroids or other anti-inflammatory drugs.

STABILITAS PEMERIKSAAN HEMATOLOGI RUTIN PADA SAMPEL DARAH YANG DIDIAMKAN PADA SUHU RUANG MENGGUNAKAN CELL-DYN RUBY

2021 ◽  
Vol 4 (1) ◽  
pp. 21
Author(s):  
Gilang Nugraha ◽  
Nur Anita Ningsih ◽  
Titik Sulifah ◽  
Sitti Fitria
Keyword(s):  
Room Temperature ◽  
Clinical Laboratory ◽  
Complete Blood Count ◽  
Laboratory Research ◽  
P Value ◽  
Blood Samples ◽  
Hematology Analyzer ◽  
Experimental Laboratory ◽  
Blood Cell Morphology ◽  
The Mean

Complete blood count (CBC) is one of the laboratory tests most often influenced by doctors. The use of a hematology analyzer offers a wider range of probe parameters. The pre-analytic stage accounts for 70% of errors, one of which is the delay of the examination. Changes in report results were reported due to changes in blood cell morphology due to EDTA additives and room temperature. The aim of this research is the disturbance of stability of the results of examination of various CBC parameters in blood samples that are left at room temperature for 24 hours using a hematology analyzer. This experimental laboratory research was conducted at the Pramita Jemur Andayani Clinical Laboratory. Blood samples were obtained from volunteers, stored at room temperature and subjected to immediate examination (control) and after a delay of 6, 12 and 24 hours (treatment). A total of 30 respondents, consisting of 8 men and 22 women. The mean age of the respondents was 22 ± 1 year. There was no difference in the results of the examination (p-value > 0.05) in the RBC, Hct MCV, MCHC, PLT and PDW. The results of the examination (p-value < 0.05) were found on Hgb, MCH, RDW, WBC, NEU, IG, MONO, EO, BASO, LYM, PLT and PDW. Delayed CBC examinations using the CELL-DYN Ruby hematology analyzer directly gave different results on several parameters ranging from 6 hours delay of examination.

Enzyme immunoassay of free thyroxin in dried blood samples on filter paper.

1985 ◽  
Vol 31 (5) ◽  
pp. 750-753 ◽  
Author(s):  
N Hata ◽  
K Miyai ◽  
M Ito ◽  
Y Endo ◽  
Y Iijimi ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Room Temperature ◽  
Normal Subjects ◽  
Blood Samples ◽  
Double Antibody ◽  
Coefficients Of Variation ◽  
The Mean ◽  
Measurable Range

Abstract We describe a double-antibody enzyme immunoassay for determination of free thyroxin (FT4) in dried blood samples on filter paper, with use of a T4-beta-D-galactosidase complex. The measurable range of FT4 concentration in two 3-mm blood discs, each of which contained about 2.7 microL of blood, was 1.9 to 93 ng/L, as determined by comparison with concentrations of FT4 in known serum standards. FT4 in blood samples dried on filter paper was stable for at least four weeks when kept dry at -20 degrees C, room temperature, or 37 degrees C. The mean coefficients of variation were 7.6% (within assay) and 6.4% (between assays). Results for FT4 by this method correlated well with those for serum determined by radioimmunoassay (r = 0.98). The proposed method can be used to differentiate persons with hyper- and hypothyroidism from normal subjects and those with abnormal concentrations of thyroxin-binding globulin. The procedure seems suited for screening studies.

Effects of storage temperature and time before centrifugation on ionized calcium in blood collected in plain vacutainer tubes and silicone-separator (SST) tubes.

1984 ◽  
Vol 30 (4) ◽  
pp. 553-556 ◽  
Author(s):  
J Toffaletti ◽  
N Blosser ◽  
K Kirvan
Keyword(s):  
Room Temperature ◽  
Storage Temperature ◽  
Ionized Calcium ◽  
Blood Collection ◽  
Healthy Individuals ◽  
Blood Samples ◽  
The Mean ◽  
Whole Blood Samples ◽  
The Stability ◽  
Blood Collection Tubes

Abstract We studied the stability of ionized calcium and pH in samples stored at either room temperature or 4 degrees C, in centrifuged and uncentrifuged blood-collection tubes and in centrifuged tubes containing a silicone-separator gel (SST tubes). At room temperature, in uncentrifuged blood from healthy individuals, mean ionized calcium usually increased no more than 10 mumol/L per hour; at 4 degrees C it did not change detectably for 70 h. This stability was fortuitous, however: the concentrations of both hydrogen and lactate ions in these samples increased, apparently with offsetting effects on the concentration of ionized calcium. Blood stored for 70 h at 4 degrees C in centrifuged SST tubes, although showing a slightly greater change in ionized calcium, had less change of pH and no change in the ionized calcium corrected to pH 7.4. In 11 heparinized whole-blood samples from eight patients in intensive care, the mean change per hour in ionized calcium and pH after storage at room temperature was +10 mumol/L and -0.04 units, respectively.

Facilitated determination of ionized calcium.

1985 ◽  
Vol 31 (2) ◽  
pp. 264-266 ◽  
Author(s):  
P Urban ◽  
B Buchmann ◽  
D Scheidegger
Keyword(s):  
Whole Blood ◽  
Ionized Calcium ◽  
Blood Samples ◽  
Intensive Care Patients ◽  
Therapeutic Measures ◽  
The Mean ◽  
Whole Blood Samples ◽  
Mean Concentration ◽  
Heparin Preparation

Abstract Using a calcium-containing heparin preparation for anticoagulation, we determined [Ca2+], the mean concentration of ionized calcium, in whole blood of 120 healthy blood-donors to be 1.23 (SD 0.04) mmol/L. Similarly, for 50 intensive-care patients selected without conscious bias, the correlation between [Ca2+] in serum (mean 1.15, SD 0.10 mmol/L) and in whole-blood samples anticoagulated with the same heparin preparation (mean 1.15, SD 0.09 mmol/L) was very good (r = 0.95). Storing samples anaerobically on ice for as long as 2 h did not alter whole-blood [Ca2+]. On the other hand, various concentrations of calcium-free heparin preparations all induced a significant decrease in measured [Ca2+]. By using whole-blood samples, rather than plasma or serum, for [Ca2+] determination with a calcium-selective electrode, repetitive measurements can be made with simple handling procedures, facilitating rapid implementation of appropriate therapeutic measures for critically ill patients.

Enzyme immunoassay of thyroxin-binding globulin in dried blood samples on filter paper.

1983 ◽  
Vol 29 (7) ◽  
pp. 1437-1440 ◽  
Author(s):  
N Hata ◽  
M Ito ◽  
H Mizuta ◽  
O Nose ◽  
K Miyai
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Room Temperature ◽  
Neonatal Hypothyroidism ◽  
Blood Samples ◽  
Coefficients Of Variation ◽  
The Mean ◽  
Positive Results ◽  
Measurable Concentration

Abstract A double-antibody enzyme immunoassay was developed for determination of thyroxin-binding globulin in dried blood samples on filter paper. The measurable concentration range of thyroxin-binding globulin in two 3-mm blood discs was 3.3 to 52 mg/L equivalent of serum (i.e., equivalent to the concentrations in known serum standards). Thyroxin-binding globulin in dried blood samples on filter paper was stable for at least four weeks when kept dry at -20 degrees C, 4 degrees C, or room temperature. The mean coefficients of variation were 6.6% (within assay) and 5.9% (between assays). The concentrations of thyroxin-binding globulin in dried blood samples determined by this method correlated well with those in serum determined by radioimmunoassay (r = 0.95) and by enzyme immunoassay (r = 0.96). This method is applicable for detecting cases of thyroxin-binding globulin deficiency and avoids the false-positive results for neonatal hypothyroidism obtained by measuring thyroxin.

scholarly journals Use of Mercury in Dental Silver Amalgam: An Occupational and Environmental Assessment

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Nadia Jamil ◽  
Mujtaba Baqar ◽  
Samar Ilyas ◽  
Abdul Qadir ◽  
Muhammad Arslan ◽  
...  
Keyword(s):  
Mercury Concentration ◽  
Preventive Measures ◽  
Potential Health ◽  
Blood Samples ◽  
Dental Clinics ◽  
The Mean ◽  
Wastewater Samples ◽  
Dental Assistants ◽  
Mean Concentration ◽  
Occupational Exposure To Mercury

The objective of this study was to assess the occupational exposure to mercury in dentistry and associated environmental emission in wastewater of Lahore, Pakistan. A total of ninety-eight blood samples were collected comprising 37 dentists, 31 dental assistants, and 30 controls. Results demonstrate that the dentistry personnel contained significantly higher mean concentration of mercury in their blood samples (dentists: 29.835 µg/L and dental assistants: 22.798 µg/L) compared to that of the controls (3.2769 µg/L). The mean concentration of mercury was found maximum in the blood samples of older age group (62.8 µg/L) in dentists and (44.3 µg/L) in dental assistants. The comparison of mercury concentration among dentists, dental assistants, and controls (pairing based on their ages) revealed that the concentration increased with the age and experience among the dentists and dental assistants. Moreover, the mercury concentration in all the studied dental wastewater samples, collected from twenty-two dental clinics, was found to be exceeding the recommended discharge limit of 0.01 mg/L. Therefore, we recommend that immediate steps must be taken to ensure appropriate preventive measures to avoid mercury vapors in order to prevent potential health hazards to dentistry personnel. Strong regulatory and administrative measures are needed to deal with mercury pollution on emergency basis.

A note on the method of replicate blood sampling when assessing the mean concentration of insulin, prolactin and growth hormone in the plasma of heifers fed different diets at different frequencies

1985 ◽  
Vol 41 (1) ◽  
pp. 113-117 ◽  
Author(s):  
W. Little ◽  
R. D. Harrison ◽  
Linda A. Williams ◽  
I. C. Hart
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Live Weight ◽  
Blood Sampling ◽  
Blood Samples ◽  
Insulin In Plasma ◽  
Residual Variation ◽  
Beet Pulp ◽  
The Mean ◽  
Mean Concentration

ABSTRACTTwo groups of six heifers of mean age 284 days and mean body weight 233 kg were offered respectively, a barley-beef (BB) diet and a diet based on dried grass and sugar beet pulp (DG) at a rate calculated to give a live-weight gain of 0·5 kg/day. Three heifers from each group were fed twice daily, and the remaining three were fed through an out-of-parlour concentrate dispenser, and, during the period of blood sampling, hourly. Following a period of adaptation, blood samples were taken hourly during 24 h and the plasma analysed for insulin, prolactin and growth hormone (GH). The experiment was repeated 28 days later using the same heifers offered the same diets except that the twice-daily fed heifers were fed hourly and vice versa.Comparing the heifers fed the BB diet and those fed the DG diet, insulin in plasma was higher (7·11 v. 3·54 mU/1, P < 0·001) and GH lower (2·48 v. 3·41 uxg/l, P < 0·05) in heifers fed the BB diet. There were no overall significant differences between the two diets for prolactin or between the two frequencies of feeding for all three hormones.The residual variation among and within heifers was used to estimate the standard error of a treatment mean for different numbers of hourly samples on different numbers of heifers. Little was to be gained by taking in a day more than eight hourly-samples for insulin and more than 12 hourlysamples for GH and prolactin. Precision was most improved by increasing the number of heifers sampled. Feeding the heifers more than twice daily did not improve precision significantly.

scholarly journals Secretion of LH, FSH and Oestradiol-17ß During the Follicular Phase of the Oestrous Cycle in the Ewe

1988 ◽  
Vol 41 (3) ◽  
pp. 303 ◽  
Author(s):  
GB Thomas ◽  
GB Martin ◽  
JR Ford ◽  
PM Moore ◽  
BK Campbell ◽  
...  
Keyword(s):  
Plasma Concentrations ◽  
Follicular Phase ◽  
Oestrous Cycle ◽  
Pulse Interval ◽  
Biphasic Response ◽  
Blood Samples ◽  
The Mean ◽  
Mean Concentration

Plasma concentrations of LH, FSH and oestradiol-17iJ were measured in blood samples taken at 15 min intervals for 48 h during the follicular phase of four Merino ewes. The amplitude of pulses of LH and the mean concentration of LH were higher at the beginning of the follicular phase, 36-24 h before the preovulatory surge of LH (amplitude 2�4 ng ml- 1, mean concentration 3�9 ng ml- 1), than at the end, 24-0 h before the preovulatory surge (amplitude 1� 2 � 0�1 ng ml- 1; mean concentration 1�4 � 0�1 ng ml- 1). There was no change in the inter-pulse interval during this time (mean 74 � 5 min). Over the same period, oestradiol levels increased from 7-8 pg ml- 1 to a peak of 10- 15 pg ml- 1. Mean FSH concentrations declined (36-24 h: 3� 6 ng ml - 1 vs 24-0 h: 1� 8 � O� 3 ng ml- 1) before rising at the time of the preovulatory surge of LH and again 24 h later. It was concluded that the biphasic response of LH to oestrogen that is seen in ovariectomized ewes may also operate during the follicular phase of the oestrous cycle in entire ewes.

Instant 99mTc-Labelled Glucoheptonate Kit for Kidney Imaging

1983 ◽  
Vol 22 (05) ◽  
pp. 246-250 ◽  
Author(s):  
M. Al-Hilli ◽  
H. M. A. Karim ◽  
M. H. S. Al-Hissoni ◽  
M. N. Jassim ◽  
N. H. Agha
Keyword(s):  
Sodium Hydroxide Solution ◽  
Normal Subjects ◽  
Organ Distribution ◽  
Stable Complex ◽  
Scintillation Camera ◽  
Ph Adjustment ◽  
The Mean ◽  
The Stability ◽  
Kidney Imaging ◽  
Mean Concentration

Gelchromatography column scanning has been used to study the fractions of reduced hydrolyzed 99mTc, 99mTc-pertechnetate and 99mTc-chelate in a 99mTc-glucoheptonate (GH) preparation. A stable high labelling yield of 99mTc-GH complex in the radiopharmaceutical has been obtained with a concentration of 40-50 mg of glucoheptonic acid-calcium salt and not less than 0.45 mg of SnCl2 2 H2O at an optimal pH between 6.5 and 7.0. The stability of the complex has been found significantly affected when sodium hydroxide solution was used for the pH adjustment. However, an alternative procedure for final pH adjustment of the preparation has been investigated providing a stable complex for the usual period of time prior to the injection. The organ distribution and the blood clearance data of 99mTc-GH in rabbits were relatively similar to those reported earlier. The mean concentration of the radiopharmaceutical in both kidneys has been studied in normal subjects for one hour with a scintillation camera and the results were satisfactory.

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