Ferrous-iron-induced oxidation in chicken liver slices as measured by hemichrome formation and thiobarbituric acid-reactive substances: Effects of dietary vitamin E and β-carotene

1993 ◽  
Vol 15 (1) ◽  
pp. 37-48 ◽  
Author(s):  
Henrik J. Andersen ◽  
Hao Chen ◽  
Lori J. Pellett ◽  
Al L. Tappel
Keyword(s):  
Vitamin E ◽  
Ferrous Iron ◽  
Thiobarbituric Acid ◽  
Chicken Liver ◽  
Dietary Vitamin ◽  
Thiobarbituric Acid Reactive Substances ◽  
Liver Slices ◽  
Β Carotene

scholarly journals Damage to DNA concurrent with lipid peroxidation in rat liver slices

1988 ◽  
Vol 252 (3) ◽  
pp. 893-896 ◽  
Author(s):  
C G Fraga ◽  
A L Tappel
Keyword(s):  
Lipid Peroxidation ◽  
Dna Damage ◽  
Rat Liver ◽  
Incubation Time ◽  
Ferrous Iron ◽  
Thiobarbituric Acid ◽  
Butylated Hydroxytoluene ◽  
Thiobarbituric Acid Reactive Substances ◽  
Butyl Hydroperoxide ◽  
Liver Slices

Lipid peroxidation and DNA damage were evaluated in liver slices incubated for 2 h at 37 degrees C with 1 mM-t-butyl hydroperoxide (t-BOOH), 1 mM-BrCCl3 or 50 microM-ferrous iron. t-BOOH induced the greatest amount of damage to DNA and increased the production of thiobarbituric acid-reactive substances (TBARS). Both phenomena depended on the incubation time. Ferrous iron induced both DNA damage and TBARS production, and BrCCl3 did not induce significant DNA damage and was the weakest TBARS inducer. Butylated hydroxytoluene at 1 mM inhibited both DNA damage and TBARS production. DNA damage and lipid peroxidation in liver slices were correlated, indicating that these events were concurrent.

Effects of Megadoses of Dietary Vitamin E on the Antioxidant Status of Rats Fed Lard or Salmon Oil

2003 ◽  
Vol 73 (4) ◽  
pp. 275-283 ◽  
Author(s):  
Flader ◽  
Brandsch ◽  
Hirche ◽  
Eder
Keyword(s):  
Vitamin E ◽  
Density Lipoprotein ◽  
Thiobarbituric Acid ◽  
Lag Time ◽  
Dietary Fats ◽  
Oxidative Activity ◽  
Tocopheryl Acetate ◽  
Ldl Oxidation ◽  
Dietary Vitamin ◽  
Thiobarbituric Acid Reactive Substances

This study was undertaken to investigate whether megadoses of vitamin E in the diet of rats can have pro-oxidative activity. Two experiments with rats were conducted in which both the dietary vitamin E concentration (Experiment 1: 100; 500; 3000; 10,000 mg all-rac-alpha-tocopheryl acetate/kg, and Experiment 2: 100; 1000; 10,000 mg all-rac-alpha-tocopheryl acetate/kg) and the type of dietary fat (lard vs. salmon oil) were varied. Experimental parameters were the concentrations of thiobarbituric acid-reactive substances, 7beta-hydroxycholesterol, the activities of several antioxidative enzymes, the concentration of glutathione in the liver, and the lag time during copper-induced low-density lipoprotein (LDL) oxidation. Increasing the dietary vitamin E concentration to 10,000 mg all-rac-alpha-tocopheryl acetate/kg led to a significant reduction of thiobarbituric acid-reactive substances in the liver after feeding salmon oil, and also to a significant reduction in 7beta-hydroxycholesterol after feeding both dietary fats. Megadoses of vitamin E (3000 and 10,000 mg all-rac-alpha-tocopheryl acetate/kg) also led to a reduction in the activity of superoxide dismutase and the concentration of glutathione in the liver of rats fed salmon oil. The lag time during LDL oxidation was independent of the dietary vitamin E concentration. The study shows that megadoses of vitamin E, far from having pro-oxidative activity, actually increase the antioxidative capacity of the liver, especially after ingestion of salmon oil.

Role of Dietary Vitamin E in Cadmium-Induced Oxidative Damage in Rabbit’s Blood, Liver and Kidneys

2003 ◽  
Vol 73 (5) ◽  
pp. 351-355 ◽  
Author(s):  
Beytut ◽  
Yuce ◽  
Kamiloglu ◽  
Aksakal
Keyword(s):  
Vitamin E ◽  
Corn Oil ◽  
Reduced Glutathione ◽  
Tap Water ◽  
Thiobarbituric Acid ◽  
Tocopheryl Acetate ◽  
Dietary Vitamin ◽  
Thiobarbituric Acid Reactive Substances ◽  
New Zealand White Rabbits

The aim of this work was to determine the effect of dietary vitamin E intake on lipid peroxidation (LPO) by measuring thiobarbituric acid reactive substances (TBARS), vitamin E and reduced glutathione (GSH) levels, and glutathione peroxidase (GSH-Px: EC 1.11.1.9) activity in plasma, red blood cells (RBC), livers, and kidneys of rabbits dosed with cadmium (Cd). Six-month-old clinically healthy New Zealand White rabbits (8 in each group) were given tap water only, containing 1 g CdCl2/L, or tap water with CdCl2 plus vitamin E (100 mg dl-alpha-tocopheryl acetate in 0.2 mL corn oil) daily for 30 days. The vitamin E level in the plasma, liver, and kidneys was significantly higher in the control than in the Cd-only group, and TBARS levels were significantly lower. There were no statistical differences between the control and Cd-only groups GSH-Px activities and GSH levels in RBC, liver, and kidneys. Vitamin E levels in plasma, liver, and kidneys and GSH-Px activity in RBC were higher in the vitamin E group than in both control and Cd-only groups. However, the TBARS levels of RBC, liver, and kidneys in vitamin E administered group were decreased. Therefore, the present study demonstrates the effectiveness of vitamin E in reducing oxidative stress in Cd-treated rabbits and suggests that reductions in increased TBARS due to Cd toxicity may be an important factor in the action of vitamin E.

Effects of hypoxia and dietary vitamin E on growth performance and oxidative status of Cirrhinus mrigala (Ham., 1822)

2017 ◽  
Vol 67 (2) ◽  
pp. 133-148 ◽  
Author(s):  
Tincy Varghese ◽  
Asim Kumar Pal ◽  
Narottam Prasad Sahu ◽  
Puthiyottil Mishal ◽  
Subrata Dasgupta
Keyword(s):  
Fatty Acids ◽  
Superoxide Dismutase ◽  
Fatty Acid ◽  
Vitamin E ◽  
Polyunsaturated Fatty Acids ◽  
Peroxide Value ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Dietary Vitamin ◽  
Thiobarbituric Acid Reactive Substances

Vitamin E is of importance for several physiological processes, some of which also apply to fish. Here, we conducted an experiment to assess the effect of environmental hypoxia and dietary vitamin E on oxidative status and tissue injury in a bottom dwelling carp,Cirrhinus mrigala(Ham., 1822). The four treatments combined oxygen availability (Normoxia/Hypoxua) and Vitamin E presence/absence. Lipid peroxidation parameters such as thiobarbituric acid reactive substances (TBAR), peroxide value (PV), polyunsaturated fatty acids/saturated fatty acid (PUFA/SFA) ratio, catalase (CAT), superoxide dismutase (SOD) and erythrocyte fragility were assessed. The results indicate that exposure to hypoxia elevates these parameters. However, the supplementation of vitamin E via the diet effectively reduced erythrocyte membrane damage (EF) and myeloperoxidase activity (MPO), which were enhanced by the exposure to hypoxia. Dietary vitamin E also improved antioxidant enzyme status in the hypoxia exposed fish, as indicated by the decreased catalase and superoxide dismutase activities. Vitamin E supplementation also compensated for increased levels of peroxide value, thiobarbituric acid reactive substances, and an increased polyunsaturated fatty acids/saturated fatty acid ratio resulting from the exposure to hypoxia. Overall, it can be concluded that fortification of vitamin E in the diet of this carp species, and possibly other fish that live under hypoxic conditions, can restore the antioxidant status and well-being to some extent.

scholarly journals Oxidative stability of tilapia feeds containing Saccharomyces cerevisiae and Spirulina platensis

2020 ◽  
Vol 50 (1) ◽  
Author(s):  
Thiago Luís Magnani Grassi ◽  
Juliana Sedlacek-Bassani ◽  
Elisa Helena Giglio Ponsano
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Vitamin E ◽  
Spirulina Platensis ◽  
Nile Tilapia ◽  
Room Temperature ◽  
Control Diet ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Oxidative Rancidity ◽  
Plastic Bags

ABSTRACT: The aim of this study was to evaluate the effect of the inclusion of microbial biomass on the oxidative rancidity of tilapia rations stored for 12 months. Treatments included a control diet and diets supplemented with either 0.01% vitamin E, 0.25 and 0.5% of Saccharomyces cerevisiae and 0.25 and 0.5% of Spirulina platensis. Experimental diets were stored in the dark inside plastic bags at room temperature (25 °C) for 12 months. The oxidative rancidity was measured as thiobarbituric acid reactive substances (TBARS). It was concluded that the inclusions of Spirulina platensis at 0.25% (1.734 ± 0.206) and 0.5% (1.629 ± 0.181) and Saccharomyces cerevisiae at 0.5% (1.459 ± 0.305) minimized the oxidative rancidity in comparation to control diet (2.843 ± 0.109) of Nile tilapia until 12 months of storage.

scholarly journals Markers of oxidative and antioxidative activity in female dogs with mammary gland tumour with and without additional vitamin E supplementation

2012 ◽  
Vol 81 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Renata Stavinohová ◽  
Jana Lorenzová ◽  
Ivana Papežíková ◽  
Ivana Borkovcová ◽  
Jakub Pfeifr ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Vitamin E ◽  
Antioxidative Activity ◽  
Thiobarbituric Acid ◽  
Thiol Groups ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Thiol ◽  
Antioxidative Status ◽  
Group 2 ◽  
Gland Tumour

The present study determined markers of oxidative and antioxidative activity in dog females affected with mammary gland tumour compared to healthy ones. The effect of additional vitamin E supplementation on oxidative and antioxidative status was evaluated as well. The study included 29 female dogs divided into 4 groups (groups 1 and 2 included females with a mammary gland tumour; groups 3 and 4 included healthy female dogs). Additional vitamin supplement containing α-tocopherol was given to the females of groups 1 and 4. Dogs from groups 1 and 2 were anaesthetized before surgery (ovariohysterectomy and mastectomy); anaesthesia was used also in group 3, but without performeing surgery. The content of vitamin E (free α-tocopherol), marker of antioxidative status, was measured in blood serum by liquid chromatography. The concentration of thiobarbituric acid reactive substances, marker of oxidative status, in serum and concentrations of protein and non-protein thiol groups, markers of oxidative and antioxidative status, in blood serum and in red blood cells were measured colorimetrically. In females with a mammary gland tumour from group 2, concentration of thiobarbituric acid reactive substances was significantly higher than 14 days after surgery and compared to healthy ones as well. In females with a mammary gland tumour from group 2, concentration of protein thiol groups in serum was significantly lower and concentration of non-protein thiol groups in serum was significantly higher than in healthy controls. The values of protein thiols in erythrocytes in females with mammary gland tumour from group 1 were significantly higher before supplementation with vitamin E. The present study revealed that females with a mammary gland tumour were more burdened with oxidative stress compared to healthy dogs. The removal of the mammary gland tumour led to improvement of oxidative and antioxidative status. This is the first report focusing on the effect of additional α-tocopherol supplementation on reducing oxidative stress by increasing antioxidative activity in females affected with mammary gland tumour; however, we did not prove it.

scholarly journals Serum Malondialdehyde is Associated with Non-Alcoholic Fatty Liver and Related Liver Damage Differentially in Men and Women

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 578 ◽  
Author(s):  
Shira Zelber-Sagi ◽  
Dana Ivancovsky-Wajcman ◽  
Naomi Fliss-Isakov ◽  
Michal Hahn ◽  
Muriel Webb ◽  
...  
Keyword(s):  
Vitamin E ◽  
Fatty Liver ◽  
Liver Damage ◽  
Thiobarbituric Acid ◽  
Abdominal Ultrasound ◽  
Cross Sectional Study ◽  
High Serum ◽  
Dietary Vitamin ◽  
Cross Sectional ◽  
Alcoholic Fatty Liver

Background: Non-alcoholic fatty liver disease (NAFLD) and steatohepatitis (NASH) are associated with increased oxidative stress and lipid peroxidation, but large studies are lacking. The aim was to test the association of malondialdehyde (MDA), as a marker of oxidative damage of lipids, with NAFLD and liver damage markers, and to test the association between dietary vitamins E and C intake and MDA levels. Methods: A cross-sectional study was carried out among subjects who underwent blood tests including FibroMax for non-invasive assessment of NASH and fibrosis. MDA was evaluated by reaction with Thiobarbituric acid and HPLC-fluorescence detection method. NAFLD was diagnosed by abdominal ultrasound. Findings: MDA measurements were available for 394 subjects. In multivariate analysis, the odds for NAFLD were higher with the rise of MDA levels in a dose–response manner, adjusting for age, gender, BMI, and lifestyle factors. Only among men, higher serum MDA was associated of higher odds for NAFLD and NASH and/or fibrosis (OR = 2.59, 95% CI 1.33–5.07, P = 0.005; OR = 2.04, 1.02–4.06, P = 0.043, respectively). Higher vitamin E intake was associated with lower odds of high serum MDA level (OR = 0.28 95% CI 0.13–0.62, P = 0.002). In conclusion, serum MDA is associated with NAFLD and markers of NASH or fibrosis among men. Dietary vitamin E may be protective among women.

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