Detection of Salmonella typhimurium in chicken meat imported in the local markets of Diwaniya city By using PCR technique

The aim of this study to detected contamination with Salmonella spp. In imported chicken meat in the local markets of Al- Diwaniyia city . to protect health of consumer and Determintion the most contaminated origin with salmonella spp. A toatl of 100 chicken meat samples collected from different origin. The bacteria cultured and isolated in enrichment and selective media . Salmonella isolates were subjected to some biochemical tests show positive productive results H2S .TSI . SIM And its give negative for indole , vo-gs Proskauer and ureas , Biochemical identification was carried out using API 20-E test ..the result showed isolation sample (33\55)60% on bismuth sulphate agar and the results of isolation on chromogenic agar were 87.8 |%(29\33) .according to reading Api20-E system the results of confirmation of isolates 92%(25\26) In this study,( 23) Salmonella isolates were detected by polymerase chain reaction (PCR) by using 16s rRNA and invA gene these primers were selected specifically for the detection of Salmonella to amplify a 406bp and 558 bp DNA fragments, respectively. Only 7 isolates out of 23 were identified as S. typhimurium the results of this study showed the highest percent of s.typhimurim isolates was ( 50%) ( 3/6) for India origin and the lowest was Turkish origin

Download Full-text

Detection of microbial contamination in chicken meat from local markets in Surabaya, East Java, Indonesia

Veterinary World ◽

10.14202/vetworld.2021.3138-3143 ◽

2021 ◽

pp. 3138-3143

Author(s):

Dhandy Koesoemo Wardhana ◽

Ajeng Erika Prihastuti Haskito ◽

Muhammad Thohawi Elziyad Purnama ◽

Devi Ayu Safitri ◽

Suwaibatul Annisa

Keyword(s):

Microbial Contamination ◽

Chicken Meat ◽

Most Probable Number ◽

Biochemical Tests ◽

Salmonella Spp ◽

Probable Number ◽

Local Markets ◽

E Coli ◽

Gram Staining ◽

Meat Samples

Background and Aim: Chicken meat can be contaminated by microorganisms anywhere in the supply chain, from farm to market, and these microorganisms can be transmitted to humans through direct contact, contact with the environment, and food consumption. The microbial contamination has a serious impact on public health. This study aimed to analyze the microbial contamination of chicken meat sampled from local markets in Surabaya, East Java, Indonesia. Materials and Methods: A total of 60 samples of fresh chicken meat obtained from 10 traditional markets (six samples per market) were examined for the presence of bacteria. Staphylococcus aureus, Salmonella spp., and Escherichia coli were identified using Gram staining, culturing, and biochemical tests. The most probable number (MPN) method was used to identify E. coli. Results: Most chicken meat samples were positive for S. aureus (58.3%), Salmonella spp. (48.3%), and E. coli (40%). The samples were considered positive for E. coli if the MPN value was higher than 1×101 CFU/g. Conclusion: High microbial contamination was found in all the chicken meat sampled from local markets in Surabaya. Such contamination can lead to foodborne diseases so, proper hygiene and sanitation standards should be followed from slaughterhouses to the end-users.

Download Full-text

Antibiotic Susceptibility of Salmonella spp Isolated from Fresh Leafy Vegetables Samples by Using Culture and Polymerase Chain Reaction Methods

European Journal of Biology and Biotechnology ◽

10.24018/ejbio.2021.2.3.199 ◽

2021 ◽

Vol 2 (3) ◽

pp. 41-45

Author(s):

S. E. Haramain ◽

S. O. Yagoub ◽

A. A. Osman

Keyword(s):

Microbial Contamination ◽

Leafy Vegetables ◽

Chain Reaction ◽

Biochemical Tests ◽

Salmonella Spp ◽

Kingdom Of Saudi Arabia ◽

Inva Gene ◽

Western Area ◽

Key Factor ◽

Polymerase Chain

Background: Microbial contamination continues to be one of the leading risks to food safety. Contaminated leafy green vegetables are the primary cause of infection among children, elderly, and immunocompromised people. The purposes of this work were to isolate and identify of Salmonella spp. in fresh leafy vegetables collected from Jeddah Central Market, Jeddah district, western area, kingdom of Saudi Arabia, estimated of the number and percentage of isolated Salmonella spp and determined the antimicrobial susceptibility of the isolated Salmonella spp. Methods: Five-hundred samples were examined for the presence of Salmonella spp, by using standard microbiological and biochemical tests. Further, detection of Salmonella spp. was done by PCR with the primers targeting invA gene, a key factor for entry of Salmonella into epithelial cells. Susceptibility of the isolated Salmonella spp was done toward thirteen different antibiotics. Results: The percentage of isolation of Salmonella spp was 1.2 % (06/500). It was isolated as (0.40%, 02/500) from Basil, (0.20%, 01/500) from Spinach, Rocket, Parsley and Chards. Two isolates (2/6, 33.3%) showed positive Salmonella invA gene (244 bp). All isolated Salmonella showed resistance to Cephalexin (30 µg/disc), Metronidazole (5 µg/disc) and Methicillin (5 µg/disc).

Download Full-text

Morphological, Biochemical and Genotypic Analysis of Zoonotic Campylobacter jejuni Isolated from Chicken Meat Samples

Indian Journal of Animal Research ◽

10.18805/ijar.b-4497 ◽

2022 ◽

Author(s):

Sumedha Bobade ◽

K. Vijayarani ◽

K.G. Tirumurugaan ◽

A. Thangavelu ◽

S. Vairamuthu

Keyword(s):

Polymerase Chain Reaction ◽

Campylobacter Jejuni ◽

Chicken Meat ◽

Poultry Meat ◽

Phenotypic Characterization ◽

Meat Production ◽

Chain Reaction ◽

Genotypic Analysis ◽

Polymerase Chain ◽

Meat Samples

Background: Campylobacter species are a leading cause of most important food-borne diarrhoeal illness worldwide while, poultry has been identified as a significant cause of Campylobacter infection in humans. C. jejuni is highly effective in colonizing chicken intestinal mucosa without causing any clinical manifestations and the consumption of poultry meat is the major source of transmission of bacteria to humans. Methods: The total of 19 chicken meat samples collected from retail markets in Chennai were screened by cultural examination, further subjected to phenotypic characterization using biochemical test and genotypic characterization using polymerase chain reaction assay targeting hip O and map A genes. Result: All the isolates showed growth on modified blood free charcoal cefoperazone deoxycholate agar media (mCCDA) and 18 (94.73%) samples showed typical morphological characteristics. The 12 (63.15%) isolates showed biochemical reactions positive. The results from polymerase chain reaction showed that 10 (83.33%) isolates were positive for C. jejuni. This study suggested that, it is essential to investigate the incidence of Campylobacter jejuni infection in poultry and the risk factors at all production stages of meat production to help reducing the disease in humans in terms of food safety.

Download Full-text

Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat

ScienceAsia ◽

10.2306/scienceasia1513-1874.2013.39.150 ◽

2013 ◽

Vol 39 (2) ◽

pp. 150 ◽

Cited By ~ 5

Author(s):

Ekkarat Rodpai ◽

Primchanien Moongkarndi ◽

Walla Tungrugsasut ◽

Rangsichon Phisannoradej ◽

Sasitorn Kanarat

Keyword(s):

Polymerase Chain Reaction ◽

Multiplex Polymerase Chain Reaction ◽

Chicken Meat ◽

Chain Reaction ◽

Salmonella Spp ◽

Polymerase Chain

Download Full-text

Pathology of Fowl Paratyphoid and Molecular Detection of Its Pathogen in Layer Chickens

Annals of Bangladesh Agriculture ◽

10.3329/aba.v24i2.55783 ◽

2021 ◽

Vol 24 (2) ◽

pp. 47-57

Author(s):

J Alam ◽

T Chakma ◽

MS Islam ◽

MT Islam ◽

MAHNA ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Salmonella Enteritidis ◽

Molecular Detection ◽

Inflammatory Cells ◽

Chain Reaction ◽

Biochemical Tests ◽

Salmonella Spp ◽

Gross Lesions ◽

Pcr Method ◽

Polymerase Chain

The study was aimed to ascertain the pathology of fowl paratyphoid and molecular detection of its causal agent (Salmonella spp) in chickens. Pathological and swab samples were collected from layers in Gazipur district, Bangladesh. For observing the gross and microscopic lesions of different organs necropsy and histopathology were done, and to isolate and identify the Salmonella spp, different bacteriological tests and Polymerase Chain Reaction (PCR) were performed. Swabs from 150 chickens showed 66% of salmonellosis. Gram’s staining of isolated bacteria showed pink colored rod shaped bacilli. In biochemical tests, Salmonella fermented dextrose, maltose, xylose, arabinose, dulcitol, mannitol except lactose and sucrose. Investigation of gross lesions at necropsy revealed hemorrhage and congestion in intestine, liver, spleen and ovaries. Necrotic foci were found in liver and spleen, and button like ulceration in cecal tonsils as well. Microscopic lesions included hemorrhage and focal necrosis in liver and spleen. Congestion and infiltrations of inflammatory cells were observed in small intestine. Ovary was hemorrhagic and there was infiltration of heterophils. Biochemically positive and isolated Salmonella organisms were confirmed by PCR method using invA and IE1 primers. The final results showed that a total of 91.7% Salmonella suspected cultures were confirmed as Salmonella Enteritidis. Ann. Bangladesh Agric. (2020) 24(2) : 47-57

Download Full-text

Screening of Salmonella spp. and Chlamydophila psittaciin parrots domiciled in Rio Branco, Acre, Brazil

Acta Veterinaria Brasilica ◽

10.21708/avb.2021.15.4.10168 ◽

2021 ◽

Vol 15 (4) ◽

pp. 339-344

Author(s):

Breno Kalyl Freitas Nascimento ◽

Luciana dos Santos Medeiros ◽

Leandro dos Santos Machado ◽

Vânia Maria França Ribeiro

Keyword(s):

Public Health ◽

Polymerase Chain Reaction ◽

Infectious Diseases ◽

Emerging Infectious Diseases ◽

Wild Animals ◽

Chain Reaction ◽

Biochemical Tests ◽

Salmonella Spp ◽

Zoonotic Pathogens ◽

Polymerase Chain

A large proportion of emerging infectious diseases (60.3%) globally are zoonotic pathogens, and of these, 71.8% originate from wild animals. Salmonellosis and psittacosis, diseases caused by Salmonella spp. and Chlamydophila psittaci, respectively, in wild animals are zoonoses with great risks to public health. Therefore, this study aimed to investigate the presence of Salmonella spp. and C. psittaci in parrots domiciled in Rio Branco, Acre. The animals in the study were raised as pets, and selection was performed based on convenience criteria. The birds were manually restrained to collect biological materials. Subsequently, conventional microbiological and biochemical tests were performed to identify Salmonella spp., and polymerase chain reaction analyses were conducted to identify C. psittaci and Salmonella spp. It was not possible to isolate Salmonella spp. and C. psittaci in the sampled birds. However, the presence of these bacteria in parrots cannot be ruled out because intermittent release and diagnostic limitations are widely described in the literature.

Download Full-text

Molecular screening of Staphylococcus aureus enterotoxins associated with samples of meat / Iraq

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i4.3590 ◽

2020 ◽

Vol 11 (4) ◽

pp. 6685-6691

Author(s):

Sura I. A. Jabuk ◽

Eman M. Jarallah

Keyword(s):

Staphylococcus Aureus ◽

Polymerase Chain Reaction ◽

Molecular Screening ◽

Chain Reaction ◽

Local Markets ◽

Fresh Frozen ◽

Polymerase Chain ◽

Staphylococcus Aureus Enterotoxins ◽

Meat Samples ◽

Encoding Genes

Staphylococcus aureus secreted many types of toxins accompanying Intestinal poisoning resulting from eating food contaminated with bacteria or their toxins. Five hundred meat samples were collected from local markets, including fresh, frozen, canned, sausage and hamburger to investigate their contamination with S.aureus and then determined their ability of these isolates to secrete enterotoxins by using polymerase chain reaction. The results showed that the ratio of isolated S.aureus is 30 (6%) and the percentage of encoding genes for toxins is 30(100%), 0(0%), 3(10), 0(0%),0(0%),3(10), 2(6.7), 1(3.3), 0(0%) and 3(10) to sea, seb, sec, sed, see, seg, see, sei, sej and sel respectively.The result shows the S.aureus isolated from contamination meat able to produce different type to enterotoxins sea, sec, seg, see, sei, and sel and present the sea toxin is the most prevalence type of staphylococcus enterotoxins.

Download Full-text

Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652001000500002 ◽

2001 ◽

Vol 43 (5) ◽

pp. 247-250 ◽

Cited By ~ 14

Author(s):

Luciana Ruschel dos SANTOS ◽

Vladimir Pinheiro do NASCIMENTO ◽

Sílvia Dias de OLIVEIRA ◽

Maristela Lovato FLORES ◽

Alexandre Pontes PONTES ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Thermal Treatment ◽

Dna Extraction ◽

Salmonella Enteritidis ◽

Chicken Meat ◽

Chain Reaction ◽

Initial Dilution ◽

Time Period ◽

Polymerase Chain ◽

Meat Samples

The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10-7, 10-8 or 10-9 CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1%) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10-9 CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.

Download Full-text

Pectobacterium spp. Associated with Bacterial Stem Rot Syndrome of Potato in Canada

Phytopathology ◽

10.1094/phyto-04-12-0083-r ◽

2012 ◽

Vol 102 (10) ◽

pp. 937-947 ◽

Cited By ~ 50

Author(s):

S. H. De Boer ◽

X. Li ◽

L. J. Ward

Keyword(s):

Gene Sequence ◽

Blackleg Disease ◽

Chain Reaction ◽

Biochemical Tests ◽

Pectobacterium Atrosepticum ◽

Physiological Characterization ◽

Specific Polymerase Chain Reaction ◽

Sequence Identification ◽

Carotovorum Subsp ◽

Polymerase Chain

Pectobacterium atrosepticum, P. carotovorum subsp. brasiliensis, P. carotovorum subsp. carotovorum, and P. wasabiae were detected in potato stems with blackleg symptoms using species- and subspecies-specific polymerase chain reaction (PCR). The tests included a new assay for P. wasabiae based on the phytase gene sequence. Identification of isolates from diseased stems by biochemical or physiological characterization, PCR, and multi-locus sequence typing (MLST) largely confirmed the PCR detection of Pectobacterium spp. in stem samples. P. atrosepticum was most commonly present but was the sole Pectobacterium sp. detected in only 52% of the diseased stems. P. wasabiae was most frequently present in combination with P. atrosepticum and was the sole Pectobacterium sp. detected in 13% of diseased stems. Pathogenicity of P. wasabiae on potato and its capacity to cause blackleg disease were demonstrated by stem inoculation and its isolation as the sole Pectobacterium sp. from field-grown diseased plants produced from inoculated seed tubers. Incidence of P. carotovorum subsp. brasiliensis was low in diseased stems, and the ability of Canadian strains to cause blackleg in plants grown from inoculated tubers was not confirmed. Canadian isolates of P. carotovorum subsp. brasiliensis differed from Brazilian isolates in diagnostic biochemical tests but conformed to the subspecies in PCR specificity and typing by MLST.

Download Full-text