scholarly journals Pengaruh Komposisi 2,4-D dan BAP Terhadap Pembentukan Kalus Eksplan Pucuk Nilam (Pogostemon Cablin Benth.) secara In Vitro dengan Pemotongan Horizontal dan Vertikal

2021 ◽  
Vol 5 (1) ◽  
pp. 9-20
Author(s):  
Khairan Khairan ◽  
Betty Mauliya Bustam ◽  
Yunita ◽  
Riska Meilinda ◽  
Raudhatul Muna
Keyword(s):  
Acetic Acid ◽  
Callus Formation ◽  
Callus Growth ◽  
Cutting Method ◽  
Highest Weight ◽  
Pogostemon Cablin

This study aims to determine the effect of 2,4-Diclorophenoxy Acetic Acid (2,4-D) and Benzyl Amino Purin (BAP) on the formation of callus of patchouli (Pogostemon cablin Benth.) shoot explants by horizontal and vertical cutting methods. The parameters that observed in this study were the percentage growth of callus, time appearance of callus, weight of callus and the morphology of callus. The results showed that horizontal cutting method was able to induce callus growth with the percentages growth of callus were 18,75%, with the time appearance of callus was at 16 days at P1; P10; P12; P13 dan P14. The highest weight of callus obtained was 0.19 grams at P8. The results also showed that the callus yielded had a yellow and cream color, with a compact and crumb textures. Meanwhile, the vertical cutting method was able to induce callus formation with the percentage growth of callus were 12,5%. The fastest time of callus appearance was obtained in P6 and P8, which was 12 day after planting with the highest weight of callus obtained was 0.05 grams at P12.   The results also showed that vertical cutting method had brown and dark-brown of callus with a compact and crumb textures.

scholarly journals Callus growth and essential oil of nilam (Pogostemon cablin (Blanco) Bth.) on the treatment with -naphtalen acetic acid and kinetin

2004 ◽  
Vol 2 (1) ◽  
pp. 9-14
Author(s):  
GUNTUR TRIMULYONO ◽  
SOLICHATUN SOLICHATUN ◽  
SOERYA DEWI MARLIANA
Keyword(s):  
Growth Rate ◽  
Acetic Acid ◽  
Essential Oil ◽  
Growth Regulation ◽  
Oil Production ◽  
Dry Weight ◽  
Callus Growth ◽  
Pogostemon Cablin ◽  
Plant Growth Regulation

The objectives of the research were to study the effect of adding of α - naphtalen acetic acid (NAA) and kinetin on callus growth and essensial oil production from nilam (Pogostemon cablin (Blanco) Bth.) callus culture. The outline of the research was the callus growth and secondary metabolite production from plant’s body could be triggered by the occurrence of plant growth regulation. The addition NAA and kinetin in culture’s medium would influence cell proliferation and synthesis of protein, so that both can induce callus growth and secondary metabolism production from the cell that have been cultured. According to the research objectives, the research was done by using in vitro callus culture method to obtain callus from explant P. cablin leaf and to induce essensial oil production. In vitro culture process consist of two stages. First stage was the callus initiation medium induced the callus from explant. The experiment was done by medium Murashige-Skoog (MS) with 2,4-D 0,5 mg/l and kinetin 0.5 mg/l; and the second stage was the medium treatment induced callus growth and essensial oil production. The research used factorial completely randomized design with two factors (NAA concentration: 0mg/l, 0,5 mg/l, 1,0 mg/l, 2,0 mg/l; and kinetin concentration: 0 mg/l, 0,5 mg/l, 1,0 mg/l, 2,0 mg/l) with 3 replicates. The collected data were qualitative data (callus morphology include texture and colour callus) and quantitative data (callus growth rate, callus dry weight and essensial oil content). Data were analyzed by Anova and followed by DMRT 5% confidence level and correlation regretion. The result of the research indicated the treatment with addition plant growth regulation (NAA and kinetin) on Murashige-Skoog’s medium had significant effect on callus growth rate but it didn’t have significant effect on callus dry weight and the increase of produced essential oil.

IN VITRO CULTURE OF WHEAT TISSUES. I. CALLUS FORMATION, ORGAN REDIFFERENTIATION AND SINGLE CELL CULTURE

1969 ◽  
Vol 11 (2) ◽  
pp. 294-304 ◽  
Author(s):  
T. Shimada ◽  
T. Sasakuma ◽  
K. Tsunewaki
Keyword(s):  
Cell Culture ◽  
Growth Factors ◽  
Single Cell ◽  
Common Wheat ◽  
Callus Formation ◽  
Casein Hydrolysate ◽  
Callus Growth ◽  
Callus Cell ◽  
Basal Media

Callus induction, organ formation from callus and single callus cell culture have been tried in wheat. Though kinetin showed no effects, supplements of 2,4-D (1~10mg/1) or IAA (50mg/1) to the basal media induced calluses from seedling roots of einkorn, emmer and common wheats, and from stem pieces of common wheat. The best callus growth was obtained when casein hydrolysate (1g/1) or coconut milk (1%) was added to the basal media. Callus growth was also vigorous when 2,4-D (0.5~2.0mg/1) was added. Root formation from callus took place in all kinds of tested media, except those containing no growth factors or supplemented with 2,4-D at high concentrations (1~5mg/1). Shoot formation occurred in six cases and no growth factors were found to be specifically effective on shoot differentiation. Two plants were restored and reached maturity. Calluses of common wheat consisted of eudiploid and aneuploid cells at almost the same frequencies. The great majority of aneuploid cells had 42 ± 3 chromosomes. The restored plants showed normal chromosome constitution. Single callus-cell suspensions were obtained by the liquid culture of seeds in a shaker. A filtrate of the single cell suspension was plated on a solid agar medium, and some colonies were formed. However, plating efficiency was very low and colony growth was slow.

scholarly journals The effect of DL-tryptophan on callus growth and alkaloid-reserpin production of pule pandak [Rauvolfia serpentina (L.) Bentham ex Kurz] in vitro

2005 ◽  
Vol 3 (2) ◽  
pp. 52-56 ◽  
Author(s):  
HENI ARYATI ◽  
ENDANG ANGGARWULAN ◽  
SOLICHATUN SOLICHATUN
Keyword(s):  
Amino Acid ◽  
Quantitative Data ◽  
Callus Formation ◽  
Leaf Explants ◽  
Treatment Phase ◽  
Optimum Concentration ◽  
Callus Growth ◽  
Second Phase ◽  
Rauvolfia Serpentina

The purposes of this research were to study the effect of amino acid DL-tryptophan at various concentrations on culture callus growth and production of alkaloid-reserpine of Rauvolfia serpentina and to determine optimum concentration of DL-tryptophan to yield maximum alkaloid reserpine of the callus. This research was consisted of three phases. First phase was to determine compatible sterilan for R. serpentina leaf explants. Second phase was to initiate/ to induce callus formation from the explants. Third phase was treatment phase to know the influence of DL-tryptophan addition on growth and alkaloid-reserpine production of the callus. Experimental design that used was Completely Randomized Design (CRD). The treatment was concentration of the amino acid DL- tryptophan with three levels concentration three restating for each level. The levels were 0 mg/L, 10 mg/L and 20 mg/L. Obtained data were analyzed quantitatively and qualitatively. Quantitative data were wet weight, dry weight, growth rate and alkaloid content of the callus at each level of concentration. Qualitative data have been measured were compatible sterilant test and callus morphology (color and texture). Quantitative data then have been analyzed by analysis of variance (ANOVA) and continued with DMRT test at level 95%. The result indicated that addition of DL- tryptophan precursor had an effect on the reduction of the callus growth of R. serpentina in media MS in vitro. Addition of DL-tryptophan precursor to produce of alkaloid-reserpine of the callus of R. serpentina in media MS in vitro and optimum concentration of DL-tryptophan precursor that must be added to yield of alkaloid-reserpine could not be determined yet, it caused by the alkaloidreserpine was not detected yet by the thin layer chromatography (TLC).

scholarly journals In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D

2017 ◽  
Vol 9 (3) ◽  
pp. 492 ◽  
Author(s):  
Iman Budisantoso ◽  
Nurul Amalia ◽  
Kamsinah Kamsinah
Keyword(s):  
Success Factors ◽  
Callus Formation ◽  
Leaf Explants ◽  
Callus Growth ◽  
Growth Time ◽  
Critical Success ◽  
Randomized Design ◽  
The Difference ◽  
Treatment Concentration

<p class="IsiAbstrakIndo"><span lang="EN-GB">The addition of growth regulator is one of the critical success factors in in vitro cultures. 2,4-D as a plant regulator in media can stimulate the cell division, enlargement of the explants and promotes the formation and growth of callus. The purpose of this study was to determine the time of callus formation and to determine the best concentration of 2.4-D in inducing the growth of callus from leaf explants of </span><em><span lang="EN-GB">Vanda</span></em><span lang="EN-GB"> sp. This research was conducted by experiment with completely randomized design, which consists of six levels of treatment concentration of 2,4-D i.e. 0 ppm; 1 ppm; 1.5 ppm; 2 ppm; 2.5 ppm; and 3 ppm. The parameters observed were the percentage of callus formation and the form of callus from </span><em><span lang="EN-GB">Vanda</span></em><span lang="EN-GB"> sp leaf explants. The results were statistically analyzed by using MINITAB program version 17. Analysis of variance (ANOVA) was performed and the difference between means score/value was separated by F test at p &lt; 0.05. The results showed that 2,4-D treatment give significant effect (F 5,12 = 3,20; p = 0,046 &lt; 0,05) on the callus growth time and its percentage. Application of 2 ppm 2.4-D was the best concentration for accelerating the callus growth time (14.3 days after planting) and increasing the percentage of callus formation (83.3%). Most of callus type were proliferative callus (36.11%) and senescence callus (11.11%). The results of this research are very important to grow the callus from Vanda leaves orchid explant because it is very diffucult to grow.</span></p>

Callus formation and organogenesis by explants of six Lycopersicon species

1983 ◽  
Vol 61 (4) ◽  
pp. 1072-1079 ◽  
Author(s):  
R. D. Locy
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Callus Formation ◽  
Adventitious Shoots ◽  
Poor Response ◽  
Shoot Formation ◽  
Callus Growth ◽  
Stem Explants ◽  
Hypocotyl Explants ◽  
Lycopersicon Species

Stem and hypocotyl explants of six Lycopersicon species, L. esculentum var. cerasiformae, L. cheesmanii, L. hirsutum, L. pimpinellifolium, L. peruvianum, and L. glandulosum, were examined for ability to form callus, adventitious shoots, and adventitious roots on media containing varying levels of indole acetic acid (IAA) and kinetin. Callus growth of L. peruvianum and L. glandulosum explants was better than that of the other species tested. Stem explants tended to form more callus than hypocotyl explants. Shoot formation by hypocotyl explants was superior to stem explants for all species and at nearly all hormone levels. Hypocotyl explants of L. peruvianum and L. glandulosum showed the highest levels of shoot formation, and L. cheesmanii and L. esculentum var. cerasiformae hypocotyl explants showed good shoot formation on media containing 3 mg/L kinetin and 0.3 or 1 mg/L indole acetic acid. No shoot formation was observed by L. hirsutum explants, and L. pimpinellifolium explants gave only a poor response. Shoot formation by stem explants was obtained only with L.. esculentum var. cerasiformae and L. glandulosum. When callus of the six species, subcultured for 63 days on a medium optimal for callus growth, was subcultured on shoot-forming media, only callus of L. peruvianum and L. glandulosum subsequently formed shoots. Callus of L. glandulosum has retained its morphogenic potential for 1 year in culture and can be used to initiate liquid suspension cultures.

scholarly journals Micropropagation of cranberry (Vaccinium macrocarpon) through shoot tip cultures: Short communication

2011 ◽  
Vol 38 (No. 4) ◽  
pp. 159-162
Author(s):  
J. Sedlák ◽  
F. Paprštein
Keyword(s):  
Acetic Acid ◽  
Woody Plant ◽  
Short Communication ◽  
Callus Formation ◽  
Vaccinium Macrocarpon ◽  
Root Induction ◽  
Multiplication Rate ◽  
Half Strength ◽  
Indole 3 Acetic Acid

The goal of this study was to determine an efficient micropropagation system for cranberry (Vaccinium macrocarpon Ait.). Cranberry cultivar Howes was successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Anderson&rsquo;s rhododendron medium (AN), half-strength Murashige and Skoog medium (half-MS) and McCown woody plant medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2&nbsp;mg/l were tested. Generally, the highest multiplication rate (2.7) was obtained for cv. Howes on AN medium with the concentration 1 mg/l of zeatin. The effects of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and naphtalen acetic acid (NAA) on root induction were tested in WPM medium. The percentage of rooting was 100% for NAA, 86% for IBA and 81% for IAA. From the tested variants, auxin NAA also promoted the highest development of good quality roots (more than 5) per shoot without callus formation.

scholarly journals In Vitro Adventitious Shoot Proliferation of Three Basil Species (Ocimum sp. L.) by Addition of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP)

2017 ◽  
Vol 4 (3) ◽  
pp. 108-115
Author(s):  
Ni Made Armini Wiendi ◽  
Devina Daratyama Putri
Keyword(s):  
Acetic Acid ◽  
Block Design ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Callus Growth ◽  
Naphthalene Acetic Acid ◽  
Randomized Block Design ◽  
Two Factors ◽  
Benzyl Amino Purine

Basils are mainly used as food flavoring and source of aromatic oil, but the leaves have also been used for herbals and cosmetics. Basil is propagated by seeds but the germination is often low in the field. This study aims to investigate the effect of different concentrations of NAA (Naphthalene Acetic Acid) and BAP (Benzyl Amino Purine) to induce the adventitious shoot proliferation of three species of basil (Ocimumsp. L.) in vitro. The research was conducted at the Tissue Culture Laboratory II of the Department of Agronomy and Horticulture, Bogor Agricultural University (IPB) from November 2016 till October 2017. This research consisted of three separate experiments with one basil species for each experiment, Lemon Basil, “Greek Minette”, and “Thai Siam Queen”. The experiments were organized in a completely randomized block design with two factors: NAA concentrations (0, 0.5, and 1 mg.L-1) and BAP (0, 1, 2, and 3 mg.L-1) using Murashige and Skoog (MS) medium supplied with 25 ml.L-1 coconut water. The three basil species responded differently to NAA and BAP in the media. The optimum concentration of NAA and BAP to induce callus growth in Lemon Basil was 0.5 mg.L-1 and 3 mg.L-1, respectively, with BAP alone showing the best effect on proliferation. In “Greek Minette”, however, the addition of NAA and BAP had no significant effect on the callus growth, proliferation, height, and number of leaf, node and root per explant. Media without growth regulators provide the best results in increasing height, explant proliferation rate, number of nodes and shoots, and percentage of proliferating shoots of “Thai Siam Queen”.Keywords: cytokinin, “Greek Minette”, Lemon Basil, “Thai Siam Queen”, synthetic auxin

scholarly journals Organogenesis and plant formation from cotyledon and callus culture of rape

2014 ◽  
Vol 49 (1-2) ◽  
pp. 5-20 ◽  
Author(s):  
Janina H. Rogozińska ◽  
Lucyna Drozdowska
Keyword(s):  
Acetic Acid ◽  
Abscisic Acid ◽  
Root Formation ◽  
Callus Formation ◽  
Inhibitory Effect ◽  
Callus Growth ◽  
Dichlorophenoxyacetic Acid ◽  
Cotyledon Explants ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid

Cotyledon explants of rape were excised from aseptically germinated seedlings and cultured during 2 weeks on M u r a s h i g e and S k o o g medium supplemented with auxins, cytokinins, auxin-cytokinin combinations and abscisic acid. Callus formation occurred on medium with 2,4-dichlorophenoxyacetic acid (2,4-D), naphthalene-l-acetic acid (NAA), indole-3-acetic acid (IAA) and on their combinations with kinetin (K) or 6-benzylaminopurine (BAP). Regeneration of roots was achieved on media with NAA, IAA and indole-3-butyric acid (IBA) and on combinations of these auxins with cytokinins. The presence of 2,4-D in the medium, though it promoted compact callus growth, had an inhibitory effect on root formation. Callus derived from the cotyledons had somewhat different requirements for growth in subculture and the root formation ability diminished in the course of the culture. Lower ABA concentrations stimulated callus growth whereas higher concentrations inhibited it similary as in the case of cotyledons. Shoot buds regenerated from the cotyledons after ca. 3 weeks on media supplemented with NAA + BAP. The 9-week-old plantlets transferred to the soil developed into complete plants. The plants which underwent vernalization formed flowers and normal seeds.

Callus Induction and Plant Regeneration in Rauvolfia Serpentina (L.) Benth Ex. Kurz.

2009 ◽  
Vol 24 ◽  
pp. 82-88 ◽  
Author(s):  
Saraswoti Aryal ◽  
Sanu Devi Joshi
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Coconut Milk ◽  
Ms Medium ◽  
Rauvolfia Serpentina ◽  
History Museum ◽  
Short Period ◽  
Murashige Skoog ◽  
Callus Tissue Culture

Rauvolfia serpentina (L.) ex. Kurz is an important medicinal plant. Callus induction and regeneration was studied from stem explant of in-vitro grown plant of Rauvolfia serpentina(L.) Benth. ex Kurz (Apocynaceae) on Murashige Skoog (1962) medium supplemented with 1mg/l 2,4-Dichlorophenocy acetic acid (2,4-D) and 1mg/l Kinetin (Kn). Vigorous growth of callus occurs after 4 weeks of culture. Callus was sub-cultured on Murashige and Skoog (MS) medium supplemented with different concentration of 2, 4-D (0.5-3.0 mg/l) and 10% coconut milk. Regeneration of plantlets occurred on MS medium containing 3 mg/1 of 2, 4-D and 10% coconut milk. These plantlets were rooted on MS medium supplemented with 1 mg/l IAA .The regenerated plantlets were able to grow on soil after short period ofacclimatization. Key words: Explant; In-vitro culture; MS medium;  2, 4 Dichlorophenoxy acetic acid; Kinetin; Callus; Tissue culture; Coconut milk. Journal of Natural History Museum Vol. 24, 2009 Page: 82-88

Advancement Of Changed Pulsincap Medicate Conveyance Arrangement Of Metronidazole For Tranquilize Focusing

2020 ◽  
Vol 2 (05) ◽  
pp. I-IV
Author(s):  
Roshni Jha ◽  
Anjali Minj
Keyword(s):  
Acetic Acid ◽  
Sodium Alginate ◽  
Guar Gum

A changed Pulsincap measurements type of metronidazole was created to target tranquilize discharge in the colon. Groups of hard gelatin cases were treated with formaldehyde keeping the tops in that capacity. Metronidazole pellets arranged by expulsion spheronization technique were consolidated into these particular container shells and stopped with polymers guar gum, hydroxypropylmethylcellulose 10K, carboxymethylcellulose sodium and sodium alginate independently at fixations 20 mg, 30 mg and 40 mg. The filled cases were totally covered with 5% cellulose acetic acid derivation phthalate to forestall variable gastric purging. All the definitions were tested to decide sedate substance and the capacity of the adjusted Pulsincap to give colon-explicit medication conveyance was surveyed by in vitro tranquilize discharge concentrates in cushion pH 1.2 for 2 h, pH 7.4 (reproduced intestinal liquid) for 3 h and pH 6.8 (animated colonic liquid) for 7 h. The outcomes showed that critical medication discharge happened simply after 5 h from the beginning of analysis.

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