scholarly journals The effect of naftalene acetic acid (NAA) on growth and essential oil contents of jasmine callus (Jasminum sambac (L.) Ait.)

2006 ◽  
Vol 4 (2) ◽  
pp. 41-44 ◽  
Author(s):  
GENINGSIH WIDYAWATI ◽  
SOLICHATUN SOLICHATUN ◽  
SOERYA DEWI MARLIYANA
Keyword(s):  
Gas Chromatography ◽  
Acetic Acid ◽  
Essential Oil ◽  
Significant Influence ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Jasminum Sambac

The objectives of the research was to determine the effect of naphthalene acetic acid (NAA) in variation concentration on the growth and the essential oil contents in Jasminum sambac (L.) Ait. callus. The research used a completely randomized design with one treatment due to NAA concentration, with five replications. The treatment was the application of NAA (0 mg/L; 0.5 mg/L; 1.0 mg/L; 1.5 mg/L; and 2.0 mg/L) on the MS media. The growth of J. sambac callus was examined after 5 weeks incubation. Essential oil contents of J. sambac callus were tested by Gas Chromatography (GC). The results of the research indicated that NAA did not give a significant influence on the growth and the essential oil contents of J. sambac callus.

scholarly journals APLIKASI INDOLE-3-BUTYRIC ACID DAN NAPHTHALENE ACETIC ACID PADA BERBAGAI BAHAN SETEK JAMBU MADU DELI HIJAU DENGAN SISTEM HIDROPONIK NFT

2018 ◽  
Vol 14 (2) ◽  
pp. 92
Author(s):  
Rizka Novi Sesanti ◽  
Hilman Hidayat ◽  
Sismanto Sismanto
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Naphthalene Acetic Acid ◽  
Hydroponic System ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design

This research is about application IBA and NAA with some cutting materials of “Madu Deli Hijau” water apple using NFT hydroponic system was aim to study the growth of water apple’s cuttings was treated by IBA and NAA 500 ppm and study the best kind of cutting materials of water apple that can growth the most.  This research was conducted using completely randomized design in two factors.  The first was consisted of 7 treatments; control (without Auxin), IBA 500 ppm, and NAA 500 ppm. The second was consisted of 4 treatments; woody branch (hardwood), semi woody (semi hardwood), and soft branch, softwood, and shoots. The results showed that the added auxin in the form of IBA and NAA 500 ppm can increase the success of madu deli hijau water apple cuttings and the cuttings from the shoots are the best for the success of madu deli hijau water apple in NFT hydroponic system.

scholarly journals Rooting Osha Crown Cuttings Using IBA and NAA

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1120A-1120
Author(s):  
Bennett J. Sondeno ◽  
Karen L. Panter
Keyword(s):  
Acetic Acid ◽  
Root Formation ◽  
Rocky Mountain ◽  
Deionized Water ◽  
Naphthalene Acetic Acid ◽  
Natural Light ◽  
Randomized Design ◽  
Ligusticum Porteri ◽  
Root Analysis ◽  
Completely Randomized Design

Osha (Ligusticum porteri) is a Rocky Mountain native frequently used as a medicinal herb. It is currently harvested largely from the wild. Studies have been under way since 2001 to find ways to propagate and produce the plant. To potentially increase rooting success of crown cuttings of osha, two different rooting hormones were used, each at two concentrations. Treatments were controls, 2500 ppm, and 5000 ppm solutions each of indole-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA). Cuttings were soaked in deionized water or treatment solutions for 2 min. After soaking cuttings were stuck in sterilized sand in 725-mL2 containers, one cutting per container. Containers were placed on a mist propagation bench at 21 °C in a completely randomized design under natural light and day lengths. Data taken were days to visible root and shoot, and presence or absence of root formation after 50 days. Results indicated only one of 70 cuttings (<1%) produced a shoot. Roots formed on 14% of control cuttings, 64% in 2500 ppm IBA, 86% in 5000 ppm IBA, 36% in 2500 ppm NAA, and 14% in 5000 ppm NAA. Days to rooting ranged from 14.9 (2500 ppm IBA) to 29.0 (5000 ppm NAA). Due to considerable variation in days to rooting, and the number of cuttings that did not root, analysis of variance showed no differences among treatments. Frequency analysis indicated differences among treatments in root presence or absence. The 2500 and 5000 ppm IBA treatments showed more root formation than the controls or either NAA treatment. This indicates IBA may enhance rooting of osha crown cuttings.

scholarly journals Physiological potential of piquin pepper seeds in response to pregermination treatments

2021 ◽  
Vol 45 ◽  
Author(s):  
Miguel Ángel Cano-González ◽  
Benjamín Abraham Ayil-Gutiérrez ◽  
Rafael Delgado-Martínez ◽  
Eduardo Osorio-Hernández ◽  
José Antonio Rangel-Lucio ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salicylic Acid ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Wild Plant ◽  
Naphthalene Acetic Acid ◽  
Distilled Water ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pharmaceutical Properties

ABSTRACT Wild piquin pepper fruits are consumed and traded because of their flavor, nutritional and pharmaceutical properties. The heterogeneous germination of its seeds has caused pregermination treatments to be unstandardized. Because of that, our goal was to evaluate pregermination treatments of piquin pepper seeds from a wild plant from the municipality of Victoria, Tamaulipas, Mexico, consisting of immersion for different periods (2, 24, 48 h) in solutions with variable concentrations of the growth regulators naphthalene acetic acid (NAA; 0.1, 1.0, 2.5 mM), salicylic acid (SA; 1.0, 5.0, 10 mM), gibberellic acid (GA3, 1.0, 5.0, 10 mM), indole-3-butyric acid (IBA; 1.0, 2.0, 3.0 mM), the commercial product Biozyme (Bioz) containing minerals and growth regulators (Bioz; 0.5, 1, 2%), and sulfuric acid (H2SO4; 2, 5, 10%) in a completely randomized design, using distilled water as the control. For each treatment, we measured the imbibition ratio, germination percentage, time to obtain 50% germination (T50), and coefficient of variation of germination time (CVt). Our results showed that the imbibition was similar in the control and the treatments, but it differed between treatments. The highest imbibition recorded in 48 h was 87%. The highest germination percentage (79.2%) was achieved with 2 h immersion in 2% Bioz, followed by 1 mM NAA (62%) and 5 mM SA (56%). T50 and CVt were 10-26 d and 0-39%, respectively. According to our results, we concluded that the pregermination treatments we assayed improved the physiological potential for the germination of piquin pepper seeds.

PENGARUH KOMBINASI INTENSITAS NAUNGAN DENGAN ZAT PENGATUR TUMBUH INDOLE BUTIRIC ACID (IBA), NAPHTHALENE ACETIC ACID (NAA), DAN VITAMIN B1 DALAM AKLIMATISASI PERTUMBUHAN BIBIT GAHARU (Aquilaria beccariana)

2013 ◽  
Vol 14 (3) ◽  
Author(s):  
Daru Mulyono
Keyword(s):  
Acetic Acid ◽  
Factorial Design ◽  
Vitamin B1 ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Optimal Formula

The objective of this research is to know the optimal formula of Indole Butiric Acid (IBA), Naphthalene Acetic Acid (NAA), Vitamine B1 and the combination with shading intensities to the acclimatization of Gaharu stump (Aquilaria beccariana). This research used Factorial Design with basic analysis of Complete Randomized Design in order to know theeffect of treatment. The research was carried out in Agroindustry and Biotechnology Laboratory, Ciampea, Bogor, from July to September 2007. The results of the research showed that after 8 weeks of treatment: (a). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 10 mg/l + Vitamine B1 1 mg/l was the best formula for increasingheight of Gaharu stump 4.660 cm. (b). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 30 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sum of Gaharu leaf stump 12.337 leafs, (c). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sumof Gaharu root stump 3.783 roots, and (d). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing length of Gaharu root stump 3.686 cm.

scholarly journals COMBINED EFFECT OF STREPTOMYCIN AND SWEET ORANGE ESSENTIAL OIL TO MEMBRANE AND ACROSOME INTEGRITY BOER GOAT FROZEN SEMEN

2019 ◽  
Vol 3 (2) ◽  
pp. 89
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Boer Goat ◽  
Randomized Design ◽  
Frozen Semen ◽  
Acrosome Integrity ◽  
Completely Randomized Design ◽  
Orange Essential Oil

One of factors that cause a bad quality of Boer Goat frozen semen is the growth of bacterial. This can be overcome by adding antibiotics such as streptomycin. To further suppress the growth of bacteria can be added other ingredients that contain antibacterials such as sweet orange essential oil. The purpose of this research is to know the percentage value of Membrane Integrity and Acrosome Integrity on Boer Goat frozen semen with addition sweet orange essential oil and streptomycin. The method used was experimental using Completely Randomized Design with 5 treatments and 5 replications. The treatment in this research is addition 0%, 0,25%; 0.5%; 0.75% and 1% sweet orange essential oil on tris yolk and streptomycin extender. The results showed the best treatment addition combination streptomycin and sweet orange essential oil to percentage Membrane Integrity and Acrosome Integrity is increase 1% sweet orange essential oil.Keywords: Boer Goat, essential oil, frozen semen, streptomycin, sweet orange.

scholarly journals Motilitas dan viabilitas spermatozoa semen sexing kambing peranakan etawa (pe) menggunakan metode sedimentasi putih telur dengan pengencer yang berbeda

2017 ◽  
Vol 5 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Yanpiterson Umbu Lele ◽  
Enike Dwi Kusumawati ◽  
Aju Tjatur Nugroho Krisnaningsih
Keyword(s):  
Significant Influence ◽  
Research Method ◽  
Egg Yolk ◽  
Egg White ◽  
Variance Analysis ◽  
Laboratory Research ◽  
Sedimentation Method ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui motilitas dan viabilitas spermatozoa semen hasil sexing kambing Peranakan Etawa (PE) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda. Materi yang digunakan dalam penelitian ini adalah semen segar kambing Peranakan Etawa (PE) berumur 2 tahun dan bobot badan 120 kg dari Balai Besar Inseminasi Buatan (BBIB)  Singosari Malang. Metode penelitian ini yang digunakan adalah penelitian laboratorium dengan menggunakan rancangan acak lengkap (RAL). Perlakuan terdiri dari semen sexing dengan menggunakan pengencer Andromed lapisan atas dan Andromed lapisan bawah dan Tris Aminomethan Kuning Telur lapisan atas dan Tris Aminomethan Kuning Telur lapisan bawah dengan masing-masing 10 ulangan. Variabel yang diamati adalah motilitas dan viabilitas spermatozoa semen sexing dengan pengencer yang berbeda, proses sexing menggunakan metode sedimentasi putih telur. Data yang diperoleh dianalisis menggunakan Analisis Varian  (ANOVA) apabila perlakuan memberikan perbedaan, maka dilanjutkan dengan Uji BNT. Hasil penelitian menunjukkan bahwa metode sedimentasi putih telur dengan pengencer yang berbeda pada kambing Peranakan Etawa (PE) memberikan pengaruh yang sangat nyata (P<0,01) terhadap motilitas dan viabilitas spermatozoa semen sexing. Motilitas dan viabilitas terbaik terdapat pada pengencer Tris aminomethan kuning telur lapisan atas sebesar 75,65%, 74,41% dan Andromed lapisan atas sebesar 65,4%, 60%. Berdasarkan hasil penelitian dapat disimpulkan bahwa kualitas spermatozoa semen sexing kambing PE dengan menggunakan pengencer Tris aminomethan memberikan hasil terbaik terhadap motilitas dan viabilitas. Berdasarkan hasil penelitian ini maka disarankan agar menggunakan pengencer Tris aminomethan kuning telur sebagai pengencer sexing spermatozoa.   ABSTRACT The purpose of this study was to determine the motility and viability of PE sperm sexing with egg white sedimentation method using different diluents. This research method used was laboratory research using a completely randomized design (CRD) treatment PE sexing sperm. The results showed that the quality of PE sperm goat with various diluents showed a significant influence (P<0,01). The variables observed were motility and viability of sperm. The data obtained were analyzed using variance analysis (ANOVA) with complete randomized design (CRD). Motility and viability at best against Tris aminomethane top layer of egg yolk 65,4% and the top layer of Andromed 75,65%, 74,41%. Based on the results it is suggested that using Tris aminomethane yolk as a diluents sexing sperm. It can be concluded that the quality of sexing sperm goat PE by using tris aminomethane diluent gives the best result on motility and viability.

scholarly journals Chemical composition of the essential oil of Psidium guajava leaves and its toxicity against Sclerotinia sclerotiorum

2018 ◽  
Vol 39 (2) ◽  
pp. 865
Author(s):  
Elizabeth Aparecida Josefi Silva ◽  
Vanessa Paula da Silva ◽  
Cassia Cristina Fernandes Alves ◽  
José Milton Alves ◽  
Edson Luiz Souchie ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Sclerotinia Sclerotiorum ◽  
Psidium Guajava ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
Randomized Design ◽  
Dry Seasons ◽  
Guava Leaves

Globally, the fungus Sclerotinia sclerotiorum is considered as one of the most important phytopathogens, since it affects the production of several economically important crops. Further, it is difficult to control, thus increasing the use of pesticides. Therefore, the search for new substances, especially those extracted from plants, has received special attention to control this plant pathogen. The objective of the present study was to evaluate the yield and chemical composition of the essential oil extracted from the fresh leaves of guava collected during the rainy and dry seasons, and also to evaluate its toxicity against S. sclerotiorum. The experiment was a completely randomized design with five treatments and six replicates. The essential oil was extracted by hydrodistillation of the leaves and analyzed by gas chromatography-flame ionization detector and gas chromatography-mass spectrometry. The main compounds identified in the essential oil were trans-caryophyllene and ?-humulene. The essential oil at a concentration of 300 ?L exhibited 90% inhibition of the mycelial growth of S. sclerotiorum. This confirmed the antifungal potential of the essential oil of the guava leaves during both the sampling seasons.

scholarly journals Chemical composition of essential oil extracted from leaves of Campomanesia adamantium subjected to different hydrodistillation times

2017 ◽  
Vol 47 (1) ◽  
Author(s):  
Juliana Dantas de Oliveira ◽  
Daniely Karen Matias Alves ◽  
Mayker Lazaro Dantas Miranda ◽  
José Milton Alves ◽  
Marcelo Nogueira Xavier ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Oil Content ◽  
Chemical Constituents ◽  
Caryophyllene Oxide ◽  
Fruit Species ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACT: Campomanesia adamantium is a native fruit species of the Cerrado and is used in food and medicines and as bee pasture. The chemical composition of essential oils obtained from plants of the same species have varying constituent proportions due to the influence of extractive factors, environmental, genetic and ontogenetic. This study aimed to identify the influence of hydrodistillation time on the content and chemical composition of essential oil extracted from the leaves of C. adamantium . Treatments consisted of five extraction times (1, 2, 3, 4, and 5h) using Clevenger with five replications in a completely randomized design. It was observed that after two hours of hydrodistillation, the essential oil content remains constant. Regarding the chemical constituents of essential oil, variation of the proportions of the compounds tested occurred at all hydrodistillation times. The compounds spathulenol oxygenated sesquiterpenes and caryophyllene oxide were the majority in the five hydrodistillation times.

scholarly journals Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro

2021 ◽  
Vol 11 (2) ◽  
pp. 114
Author(s):  
Nurkapita Nurkapita ◽  
Riza Linda ◽  
Zulfa Zakiah
Keyword(s):  
Tissue Culture ◽  
Zea Mays ◽  
Acetic Acid ◽  
Seed Extract ◽  
Naphthalene Acetic Acid ◽  
Shoot Height ◽  
Culture Techniques ◽  
Corn Seed ◽  
Randomized Design

(Article History: Received February 18, 2021; Revised April 27, 2021; Accepted May 19, 2021) ABSTRAKPerkembangbiakan anggrek secara generatif alami membutuhkan bantuan jamur mikoriza untuk perkecambahan biji, sedangkan usaha perbanyakan konvensional memerlukan waktu lama untuk memperoleh tanaman dalam jumlah banyak. Salah satu alternatif untuk perbanyakan anggrek hitam (Coelogyne pandurata Lindl.) adalah melalui multiplikasi tunas anggrek secara in vitro. Tujuan penelitian adalah untuk membuktikan pengaruh pemberian NAA (Naphthalene Acetic Acid) dan ekstrak biji jagung (Zea mays) terhadap multiplikasi tunas anggrek hitam. Penelitian dilakukan di Laboratorium Kultur Jaringan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor perlakuan. Faktor pertama adalah NAA terdiri dari 5 taraf konsentrasi yaitu A0 (0 M/ kontrol) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) dan A4 (5x10-6 M ) dan faktor ekstrak biji jagung (B) dengan 5 taraf konsentrasi yaitu B0 (0%), B1 (2,5%), B2 (5%); B3 (7,5%) dan B4 (10%). Pemberian kombinasi NAA dan ekstrak biji jagung berpengaruh nyata terhadap semua parameter yaitu jumlah tunas, jumlah daun, dan tinggi tunas. Hasil terbaik rerata jumlah tunas pada perlakuan A4+B4 yaitu 5x10-6M NAA+10% ekstrak biji jagung. Hasil terbaik pada rerata jumlah daun pada perlakuan A2+B2 yaitu 5x10-7M NAA+5% ekstrak biji jagung dan hasil terbaik pada rerata tinggi tunas pada perlakuan A1+B1 yaitu 10-7M NAA+2,5% ekstrak biji jagung.Kata Kunci: multiplikasi; tunas anggrek hitam; ekstrak biji jagung; NAA. ABSTRACTGenerative reproduction of orchid plants it takes a requires the help of mycorriza mushrooms for seed germination, whereas conventional propagation business takes a long time to obtain large quantities of plants. One alternative to the propagation black orchids (Coelogyne pandurata Lindl.) is required through tissue culture techniques. The purpose of this study is to find the influence and concentration corn seed extract (Zea mays) and NAA (Naphthalene Acetic Acid) on the multiplication black orchids. This research was conducted in the tissue culture laboratory Biology Department Faculty of Mathematics and Natural Sciences Tanjungpura University Pontianak. The study used a Complete Randomized Design (RAL) of factorial patterns with two treatment factors. The first factor is that the NAA consists of 5 concentration levels  A0 (0 M) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) and A4 (5x10-6 M ) and the second factor is that corn seed extract of 5 levels concentratio B0(0%), B1 (2,5%), B2 (5%); B3 (7,5%) and B4 (10%). The administration NAA and corn seed extract in combination has a real effect on all parameters namely the number shoots, the number leaves, and the height shoots. The best results where the average number of shoots in the treatment of A2+B2 namely 5x10-6M NAA + 10% corn seed extract. The best results average number of leaves in the treatment  A2+B2 namely 5x10-7M NAA + 5% corn seed extract and in the best results for shoot height in the treatment of A1+B1 namely 10-7M NAA + 2.5% corn seed extract.Keywords: Multiplication; black orchid’s shoot; corn  seed extract; NAA

scholarly journals Effects of topping times on growth and yield of three sweet basil (Ocimum basilicum L.) varieties cultivated in the net house

2018 ◽  
Vol 17 (04) ◽  
pp. 19-27
Author(s):  
Tam T. M. Pham
Keyword(s):  
Essential Oil ◽  
Oil Content ◽  
Growth And Yield ◽  
High Yield ◽  
Sweet Basil ◽  
Randomized Design ◽  
Good Taste ◽  
Completely Randomized Design ◽  
Basil Oil ◽  
Development Center

The study was carried out from June to December, 2014. The objective of this study was to determine a suitable topping time for sweet basil variety grown in the net house at Research and Development Center for Hi-Tech Agriculture Ho Chi Minh City. A two-factorial experiment was designed by the CRD (Completely Randomized Design) with three replications. Factor A consisted of three sweet basil varieties (TN12 as Control, TN33, and TN39) and the factor B was the topping times including untopping (the control), the topping when sweet basil at the four-leaves, at the six-leaves and at the eight-leaves period. The results showed that variety TN39 was suitable for extracting basil-oil because of the highest yield (3,365.4 kg/1,000 m2) and high total essential oil content (0.25%). Variety TN12 could be grown for fresh-eating spicy. The variety TN12 resulted in the fairly high yield (1,955.4 kg/1,000 m2) and total essential oil content (0.20%) with good taste. Topping the basil plant at the six-leaves period reached the highest yield of 2,172.3 kg/1,000 m2.

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