scholarly journals Rooting Osha Crown Cuttings Using IBA and NAA

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1120A-1120
Author(s):  
Bennett J. Sondeno ◽  
Karen L. Panter
Keyword(s):  
Acetic Acid ◽  
Root Formation ◽  
Rocky Mountain ◽  
Deionized Water ◽  
Naphthalene Acetic Acid ◽  
Natural Light ◽  
Randomized Design ◽  
Ligusticum Porteri ◽  
Root Analysis ◽  
Completely Randomized Design

Osha (Ligusticum porteri) is a Rocky Mountain native frequently used as a medicinal herb. It is currently harvested largely from the wild. Studies have been under way since 2001 to find ways to propagate and produce the plant. To potentially increase rooting success of crown cuttings of osha, two different rooting hormones were used, each at two concentrations. Treatments were controls, 2500 ppm, and 5000 ppm solutions each of indole-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA). Cuttings were soaked in deionized water or treatment solutions for 2 min. After soaking cuttings were stuck in sterilized sand in 725-mL2 containers, one cutting per container. Containers were placed on a mist propagation bench at 21 °C in a completely randomized design under natural light and day lengths. Data taken were days to visible root and shoot, and presence or absence of root formation after 50 days. Results indicated only one of 70 cuttings (<1%) produced a shoot. Roots formed on 14% of control cuttings, 64% in 2500 ppm IBA, 86% in 5000 ppm IBA, 36% in 2500 ppm NAA, and 14% in 5000 ppm NAA. Days to rooting ranged from 14.9 (2500 ppm IBA) to 29.0 (5000 ppm NAA). Due to considerable variation in days to rooting, and the number of cuttings that did not root, analysis of variance showed no differences among treatments. Frequency analysis indicated differences among treatments in root presence or absence. The 2500 and 5000 ppm IBA treatments showed more root formation than the controls or either NAA treatment. This indicates IBA may enhance rooting of osha crown cuttings.

scholarly journals APLIKASI INDOLE-3-BUTYRIC ACID DAN NAPHTHALENE ACETIC ACID PADA BERBAGAI BAHAN SETEK JAMBU MADU DELI HIJAU DENGAN SISTEM HIDROPONIK NFT

2018 ◽  
Vol 14 (2) ◽  
pp. 92
Author(s):  
Rizka Novi Sesanti ◽  
Hilman Hidayat ◽  
Sismanto Sismanto
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Naphthalene Acetic Acid ◽  
Hydroponic System ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design

This research is about application IBA and NAA with some cutting materials of “Madu Deli Hijau” water apple using NFT hydroponic system was aim to study the growth of water apple’s cuttings was treated by IBA and NAA 500 ppm and study the best kind of cutting materials of water apple that can growth the most.  This research was conducted using completely randomized design in two factors.  The first was consisted of 7 treatments; control (without Auxin), IBA 500 ppm, and NAA 500 ppm. The second was consisted of 4 treatments; woody branch (hardwood), semi woody (semi hardwood), and soft branch, softwood, and shoots. The results showed that the added auxin in the form of IBA and NAA 500 ppm can increase the success of madu deli hijau water apple cuttings and the cuttings from the shoots are the best for the success of madu deli hijau water apple in NFT hydroponic system.

scholarly journals Physiological potential of piquin pepper seeds in response to pregermination treatments

2021 ◽  
Vol 45 ◽  
Author(s):  
Miguel Ángel Cano-González ◽  
Benjamín Abraham Ayil-Gutiérrez ◽  
Rafael Delgado-Martínez ◽  
Eduardo Osorio-Hernández ◽  
José Antonio Rangel-Lucio ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salicylic Acid ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Wild Plant ◽  
Naphthalene Acetic Acid ◽  
Distilled Water ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pharmaceutical Properties

ABSTRACT Wild piquin pepper fruits are consumed and traded because of their flavor, nutritional and pharmaceutical properties. The heterogeneous germination of its seeds has caused pregermination treatments to be unstandardized. Because of that, our goal was to evaluate pregermination treatments of piquin pepper seeds from a wild plant from the municipality of Victoria, Tamaulipas, Mexico, consisting of immersion for different periods (2, 24, 48 h) in solutions with variable concentrations of the growth regulators naphthalene acetic acid (NAA; 0.1, 1.0, 2.5 mM), salicylic acid (SA; 1.0, 5.0, 10 mM), gibberellic acid (GA3, 1.0, 5.0, 10 mM), indole-3-butyric acid (IBA; 1.0, 2.0, 3.0 mM), the commercial product Biozyme (Bioz) containing minerals and growth regulators (Bioz; 0.5, 1, 2%), and sulfuric acid (H2SO4; 2, 5, 10%) in a completely randomized design, using distilled water as the control. For each treatment, we measured the imbibition ratio, germination percentage, time to obtain 50% germination (T50), and coefficient of variation of germination time (CVt). Our results showed that the imbibition was similar in the control and the treatments, but it differed between treatments. The highest imbibition recorded in 48 h was 87%. The highest germination percentage (79.2%) was achieved with 2 h immersion in 2% Bioz, followed by 1 mM NAA (62%) and 5 mM SA (56%). T50 and CVt were 10-26 d and 0-39%, respectively. According to our results, we concluded that the pregermination treatments we assayed improved the physiological potential for the germination of piquin pepper seeds.

scholarly journals The effect of naftalene acetic acid (NAA) on growth and essential oil contents of jasmine callus (Jasminum sambac (L.) Ait.)

2006 ◽  
Vol 4 (2) ◽  
pp. 41-44 ◽  
Author(s):  
GENINGSIH WIDYAWATI ◽  
SOLICHATUN SOLICHATUN ◽  
SOERYA DEWI MARLIYANA
Keyword(s):  
Gas Chromatography ◽  
Acetic Acid ◽  
Essential Oil ◽  
Significant Influence ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Jasminum Sambac

The objectives of the research was to determine the effect of naphthalene acetic acid (NAA) in variation concentration on the growth and the essential oil contents in Jasminum sambac (L.) Ait. callus. The research used a completely randomized design with one treatment due to NAA concentration, with five replications. The treatment was the application of NAA (0 mg/L; 0.5 mg/L; 1.0 mg/L; 1.5 mg/L; and 2.0 mg/L) on the MS media. The growth of J. sambac callus was examined after 5 weeks incubation. Essential oil contents of J. sambac callus were tested by Gas Chromatography (GC). The results of the research indicated that NAA did not give a significant influence on the growth and the essential oil contents of J. sambac callus.

PENGARUH KOMBINASI INTENSITAS NAUNGAN DENGAN ZAT PENGATUR TUMBUH INDOLE BUTIRIC ACID (IBA), NAPHTHALENE ACETIC ACID (NAA), DAN VITAMIN B1 DALAM AKLIMATISASI PERTUMBUHAN BIBIT GAHARU (Aquilaria beccariana)

2013 ◽  
Vol 14 (3) ◽  
Author(s):  
Daru Mulyono
Keyword(s):  
Acetic Acid ◽  
Factorial Design ◽  
Vitamin B1 ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Optimal Formula

The objective of this research is to know the optimal formula of Indole Butiric Acid (IBA), Naphthalene Acetic Acid (NAA), Vitamine B1 and the combination with shading intensities to the acclimatization of Gaharu stump (Aquilaria beccariana). This research used Factorial Design with basic analysis of Complete Randomized Design in order to know theeffect of treatment. The research was carried out in Agroindustry and Biotechnology Laboratory, Ciampea, Bogor, from July to September 2007. The results of the research showed that after 8 weeks of treatment: (a). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 10 mg/l + Vitamine B1 1 mg/l was the best formula for increasingheight of Gaharu stump 4.660 cm. (b). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 30 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sum of Gaharu leaf stump 12.337 leafs, (c). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sumof Gaharu root stump 3.783 roots, and (d). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing length of Gaharu root stump 3.686 cm.

scholarly journals Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro

2021 ◽  
Vol 11 (2) ◽  
pp. 114
Author(s):  
Nurkapita Nurkapita ◽  
Riza Linda ◽  
Zulfa Zakiah
Keyword(s):  
Tissue Culture ◽  
Zea Mays ◽  
Acetic Acid ◽  
Seed Extract ◽  
Naphthalene Acetic Acid ◽  
Shoot Height ◽  
Culture Techniques ◽  
Corn Seed ◽  
Randomized Design

(Article History: Received February 18, 2021; Revised April 27, 2021; Accepted May 19, 2021) ABSTRAKPerkembangbiakan anggrek secara generatif alami membutuhkan bantuan jamur mikoriza untuk perkecambahan biji, sedangkan usaha perbanyakan konvensional memerlukan waktu lama untuk memperoleh tanaman dalam jumlah banyak. Salah satu alternatif untuk perbanyakan anggrek hitam (Coelogyne pandurata Lindl.) adalah melalui multiplikasi tunas anggrek secara in vitro. Tujuan penelitian adalah untuk membuktikan pengaruh pemberian NAA (Naphthalene Acetic Acid) dan ekstrak biji jagung (Zea mays) terhadap multiplikasi tunas anggrek hitam. Penelitian dilakukan di Laboratorium Kultur Jaringan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor perlakuan. Faktor pertama adalah NAA terdiri dari 5 taraf konsentrasi yaitu A0 (0 M/ kontrol) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) dan A4 (5x10-6 M ) dan faktor ekstrak biji jagung (B) dengan 5 taraf konsentrasi yaitu B0 (0%), B1 (2,5%), B2 (5%); B3 (7,5%) dan B4 (10%). Pemberian kombinasi NAA dan ekstrak biji jagung berpengaruh nyata terhadap semua parameter yaitu jumlah tunas, jumlah daun, dan tinggi tunas. Hasil terbaik rerata jumlah tunas pada perlakuan A4+B4 yaitu 5x10-6M NAA+10% ekstrak biji jagung. Hasil terbaik pada rerata jumlah daun pada perlakuan A2+B2 yaitu 5x10-7M NAA+5% ekstrak biji jagung dan hasil terbaik pada rerata tinggi tunas pada perlakuan A1+B1 yaitu 10-7M NAA+2,5% ekstrak biji jagung.Kata Kunci: multiplikasi; tunas anggrek hitam; ekstrak biji jagung; NAA. ABSTRACTGenerative reproduction of orchid plants it takes a requires the help of mycorriza mushrooms for seed germination, whereas conventional propagation business takes a long time to obtain large quantities of plants. One alternative to the propagation black orchids (Coelogyne pandurata Lindl.) is required through tissue culture techniques. The purpose of this study is to find the influence and concentration corn seed extract (Zea mays) and NAA (Naphthalene Acetic Acid) on the multiplication black orchids. This research was conducted in the tissue culture laboratory Biology Department Faculty of Mathematics and Natural Sciences Tanjungpura University Pontianak. The study used a Complete Randomized Design (RAL) of factorial patterns with two treatment factors. The first factor is that the NAA consists of 5 concentration levels  A0 (0 M) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) and A4 (5x10-6 M ) and the second factor is that corn seed extract of 5 levels concentratio B0(0%), B1 (2,5%), B2 (5%); B3 (7,5%) and B4 (10%). The administration NAA and corn seed extract in combination has a real effect on all parameters namely the number shoots, the number leaves, and the height shoots. The best results where the average number of shoots in the treatment of A2+B2 namely 5x10-6M NAA + 10% corn seed extract. The best results average number of leaves in the treatment  A2+B2 namely 5x10-7M NAA + 5% corn seed extract and in the best results for shoot height in the treatment of A1+B1 namely 10-7M NAA + 2.5% corn seed extract.Keywords: Multiplication; black orchid’s shoot; corn  seed extract; NAA

scholarly journals Accelerated ageing as a vigour test on chickpea seeds

2020 ◽  
pp. 339
Author(s):  
Letícia Betânia Xavier Dias ◽  
Pedro Afonso de Melo Queiroz ◽  
Lara Bernardes da Silva Ferreira ◽  
Marco Antônio Moreira de Freitas ◽  
Érica Fernandes Leão Araújo ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Salt Solution ◽  
Saline Solution ◽  
Deionized Water ◽  
Accelerated Ageing ◽  
Ageing Test ◽  
Randomized Design ◽  
Initial Characterization ◽  
Completely Randomized Design ◽  
Saline Test

The objective of this work was to establish the temperature and the period of exposure needed to perform the traditional accelerated ageing test with saturated NaCl salt solution on chickpea seeds. Eight seed lots from two cultivars, including four seeds each, were subjected to germination and vigour tests for initial characterization. Following the methodology of the traditional accelerated ageing test. The seeds were placed on stainless steel screens in boxes with 40 mL deionized water. They were kept at 41 and 45º Celsius for 24 and 48 hours. Furthermore, the accelerated ageing test using [40 g of NaCl in 100 mL of water, thus providing 76% of relative humidity in air] NaCl (salt) solution in place of the deionized water was performed. Aged seeds were sowed in germination paper and kept in a germination chamber at 41º and 45º Celsius. The evaluations were performed five and eight days after sowing. The statistical analysis was performed with a completely randomized design and four replications. The traditional ageing of both cultivars showed a water content higher than the tolerated limits at 48 hour period and 41 and 45 °C. In the saturated saline test, the cultivar BRS Aleppo produced consistent results at temperatures 45 °C for 24 h. The cultivar Cícero showed a low correlation with the initial characterization as the results of accelerated ageing. Thus, for ‘BRS Aleppo’, the recommended accelerated ageing methodology was application of saturated saline solution, when seed exposed to 41 °C for 24 hours with germination evaluation at the first count date. For 'Cicero', satisfactory results were not obtained, and new studies are required to establish a methodology.

scholarly journals VEGETATIVE PROPAGATION OF Spondias tuberosa E Spondias dulcis WITH THE USE OF IMMERSION IN INDOLE ACETIC ACID

2019 ◽  
Vol 32 (4) ◽  
pp. 858-866
Author(s):  
GABRIELA TEODORO ROCHA ◽  
AYURE GOMES DA SILVA ◽  
JULIANA BEZERRA MARTINS ◽  
NEI PEIXOTO ◽  
FABRICIO RODRIGUES
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Indole Acetic Acid ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Spondias Tuberosa ◽  
Completely Randomized Design ◽  
Low Efficiency ◽  
Indole 3 Acetic Acid ◽  
Total Fresh Weight

ABSTRACT The objective of this work was to evaluate the effect of six indole-3-acetic acid (IAA) concentrations and three immersion times in IAA solutions on the vegetative propagation of Spondias dulcis and Spondias tuberosa by stem cuttings. The experiment was conducted in a greenhouse, using a completely randomized design, with a 6×3 factorial arrangement consisting of six indole-3-acetic acid concentrations (0, 2, 4, 6, 8, and 10 g L-1) and three immersion times (8, 16, and 24 seconds), with ten cuttings of S. dulcis and S. tuberosa per plot, and three replications. Percentage of surviving cuttings, and percentage of rooted cuttings, number of leaf buds, number of roots per cutting, root length, and total fresh weight were evaluated at 180 days after planting. Data were subjected to analysis of variance by the F test and to regression analysis. Vegetative propagation from woody cuttings of S. dulcis is not viable to produce seedlings, thus, new studies on this technique for this species are needed. Vegetative propagation from woody cuttings of S. tuberosa is satisfactory, but with low efficiency for improvement and emergence of new shoots and roots; it is more effective when using a concentration of 10 g L-1 of indole-3-acetic acid and immersion time in the solution of 16 seconds.

scholarly journals Profiling of Collagens from Swim Bladder of Catfish (Pangasius sp.) by Acid Extraction

2019 ◽  
Vol 22 (2) ◽  
pp. 327-339
Author(s):  
Gressty Sari Br Sitepu ◽  
Joko Santoso ◽  
Wini Trilaksani
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Triple Helix ◽  
Extraction Process ◽  
Raw Material ◽  
Acid Extraction ◽  
Swim Bladder ◽  
Acid Pretreatment ◽  
Randomized Design ◽  
Completely Randomized Design

Swim bladders of catfish contain high protein, therefore it is can be used as a raw material for collagen. The study aims to determine the chemical characteristics of swim bladders, the pretreatment of non-collagen, extraction of collagen dissolves acid and to evaluate the characteristics of collagen. The method of this study is KOH pretreatment with a concentration of 0,05; 0,1; and 0,15 for 12 hours. The extraction process is done by soaking the sample in a solution of acetic acid with a concentration of 0,25; 0,5 and 0,75 M (ratio 1:10; b/v) and extraction time for 24; 48; and 72 hours at 4oC. The experimental design used for alkaline and acetic acid pretreatment were factorial completely randomized design. The result showed that the protein content of swim bladder was 85,26% (db), the profile of amino acids were dominated by three amino acids namely glycine (56,85 mg/g), prolyne (31,03 mg/g), and alanyne (23,85 mg/g). Using 0,05 M KOH for 8 h was selected as the best pretreatment method for collagen extraction. Extraction method using 0,50 M acetic for 48 h resulted he best collagen which revealed the existence of a triple helix structure and had Tg 84oC.

scholarly journals Nutritional value of sugarcane silages added with different acetic acid doses

2019 ◽  
Vol 40 (5Supl1) ◽  
pp. 2387
Author(s):  
Diego Lucas Soares de Jesus ◽  
João Paulo Sampaio Rigueira ◽  
Flávio Pinto Monção ◽  
Wagner Sousa Alves ◽  
Marielly Maria Almeida Moura ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Dry Matter ◽  
Nutritional Value ◽  
Glacial Acetic Acid ◽  
Saccharum Officinarum ◽  
Quadratic Regression ◽  
Yeast Population ◽  
Randomized Design ◽  
Fermentation Quality ◽  
Completely Randomized Design

The objective was to evaluate the effect of inclusion of acetic acid during the ensiling of sugarcane (Saccharum officinarum) on fermentation quality and nutritional value. The treatments consisted of sugarcane silages, variety (IAC 86-2480) additives with four inclusion levels of glacial acetic acid (1.5, 3.0, 4.5 and 6.0 % in natural matter) plus the control. A completely randomized design was used, being 5 treatments and 6 replicates. The glacial acetic acid showed a pH 2.9 (0,1M). There was a reduction of 0.07 units in the pH of the sugarcane silage for each 1% inclusion of acetic acid (P < 0.01). The N-NH3 concentration was not modified in the ensiled mass of the treatments, with a mean of 0.45% in dry matter (P =0.91). The averages of effluent losses were adjusted to the quadratic regression model with the application of acetic acid (P < 0.01). There was a reduction in the yeast population in the order of 0.44 log UFC/g of silage (P < 0.01). The addition of acetic acid in sugarcane silage reduces fermentative losses, the yeast population and improves the nutritional value in doses from 1.5% of natural matter.

scholarly journals Effectiveness of Alkaline Pretreatment and Acetic Acid Hydrolysis on the Characteristics of Collagen from Fish Skin of Snakehead

2015 ◽  
Vol 18 (3) ◽  
Author(s):  
Wulandari Wulandari ◽  
Pipih Suptijah
Keyword(s):  
Acetic Acid ◽  
Alkaline Pretreatment ◽  
Fish Skin ◽  
Acid Pretreatment ◽  
Alternative Source ◽  
Randomized Design ◽  
Collagen Protein ◽  
Sheet Structure ◽  
Completely Randomized Design ◽  
Β Sheet

<p>Fish skin is one of marine byproducts potential for alternative source of collagen. This study<br />investigated the effectiveness of alkaline and acetic acid pretreatment on the characteristics of<br />collagen from skin snakehead fish. The concentrations of alkaline pretreatment were 0.05; 0.1; 0.15<br />and 0.2 M for 2, 4, 6, 8, 10 and 12 hours, acetic acid concentrations were 0.05 M, 0.1 M, 0.15 M and<br />0.2 M for 1 and 2 hours. The experimental design used for alkaline and acetic acid pretreatment<br />was factorial completely randomized design. The result showed that the concentration of alkaline<br />0.05 M for 6 hours have significant effect on the elimination of non-collagen protein (p&lt;0.05),<br />whereas for the optimum acetic acid at a concentration 0.1 M for 2 hours significantly different<br />on solubility and swelling. Extraction yields of collagen was 16%, with characteristics of whiteness<br />66.67%, protein content 96.21%, viscosity 10 cP, Tmax 159.9oC and glass transition temperature<br />78.55oC. The dominant amino acid composition were glycine (27.11%), proline (13.87%) and<br />alanine (12.58%). Functional groups collagen from skin snakehead fish has β-sheet structure<br />which is a characteristic of collagen.</p>

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