scholarly journals ESTIMATION OF EST-SSR AND SRAP-RGA MARKERS FOR GENOTYPING YELLOW LUPIN (LUPINUS LUTEUS L.)

Author(s):  
E. N. Sysoliatin ◽  
N. V. Anisimova ◽  
O. G. Babak ◽  
V. S. Anokhina ◽  
I. Y. Romanchuk ◽  
...  

14 SRAP-RGA primer combinations and 8 EST-SSR markers were tested on the collection of 10 different yellow lupine samples. The most efficient primers for intravarietal differentiation of yellow lupine were distinguished according their parameters. Reliable correlations were determined between the presence of EST-SSR fragments and some characteristics of productivity in hybrid (F2 ) yellow lupine populations. 

2012 ◽  
Vol 58 (2) ◽  
pp. 407-416
Author(s):  
Bożena Cwalina-Ambroziak ◽  
Tomasz P. Kurowski

The object of the experiment were seeds of two traditional cultivars of yellow lupin (Juno and Amulet) cultivated in 1999 in two crop-rotation with 20% and 33% yellow lupine contribution. The quantitative and qualitative composition of the fungal community colonizing the seeds were determined in the laboratory conditions after 0.5-, 1.5- and 2.5-year of storage time. In total 1077 fungal colonies were isolated from the lupin seeds. Fungi representing the species of <i>Penicillium</i> - 29.3%, <i>Alternaria alternata</i> - 26.7% and <i>Rhizopus nigricans</i> - 12.7% were isolated most widely. Among the fungi pathogenic to lupin, the species of <i>Colletotrichum gloeosporioides</i> (16.3% isolates) was dominant. The crop rotation with 20% lupin reduced the number of fungal colonies colonizing the seeds including the pathogens from the species of <i>C. gloeosporioides</i>. Seed disinfection decreased the total number of fungal colonies isolated from both cultivars. Higher number of <i>C. gloeosporioides</i> isolates was found in the combination with disinfected seeds. More fungal colonies were obtained from seeds of cv. Amulet than from those of cv. Juno. The storage duration had an effect on the population and the composition of species of fungi isolated from seeds of yellow lupine. With longer storage population of <i>Penicillium</i> spp. and <i>Rhizopus</i> spp. increased, whereas the population of <i>C. gloeosporioides</i> decreased.


2002 ◽  
Vol 57 (9-10) ◽  
pp. 811-816 ◽  
Author(s):  
Yasufumi Katagiri ◽  
Yasuyuki Hashidoko ◽  
Satoshi Tahara

Quantification of the flavonoids in yellow lupin (Lupinus luteus; Leguminosae) seedlings revealed that a flavone glucoside, 7-O-β-(2-O-β-rhamnosyl)glucosyl-4′,5,7-trihydroxyflavone (apigenine 7-O-β-neohesperidoside), is rich in the epicotyl and cotyledon. In hypocotyls and roots, 8-C-β-glucosyl-4′,5,7-trihydroxyisoflavone (genistein 8-C-β-glucoside) was a predominant flavonoid constituent. The roles of the localized flavonoids are briefly discussed relating to defense against biotic and abiotic external stresses.


2000 ◽  
Vol 51 (6) ◽  
pp. 701 ◽  
Author(s):  
C. L. Davies ◽  
D. W. Turner ◽  
M. Dracup

We studied the adaptation of narrow-leafed lupin (Lupinus angustifolius) and yellow lupin (L. luteus) to waterlogging because yellow lupin may have potential as a new legume crop for coarse-textured, acidic, waterlogging-prone areas in Western Australia. In a controlled environment, plants were waterlogged for 14 days at 28 or 56 days after sowing (DAS). Plants were more sensitive when waterlogged from 56 to 70 DAS than from 28 to 42 DAS, root growth was more sensitive than shoot growth, and leaf expansion was more sensitive than leaf dry weight accumulation. Waterlogging reduced the growth of narrow-leafed lupin (60–81%) more than that of yellow lupin (25–56%) and the response was more pronounced 2 weeks after waterlogging ceased than at the end of waterlogging. Waterlogging arrested net root growth in narrow-leafed lupin but not in yellow lupin, so that after 2 weeks of recovery the root dry weight of yellow lupin was the same as that of the control plants but in narrow-leafed lupin it was 62% less than the corresponding control plants. Both species produced equal amounts of hypocotyl root when waterlogged from 28 to 42 DAS but yellow lupin produced much greater amounts than narrow-leafed lupin when waterlogged from 56 to 70 DAS.


2011 ◽  
Vol 38 (11) ◽  
pp. 910 ◽  
Author(s):  
Helen Bramley ◽  
Stephen D. Tyerman ◽  
David W. Turner ◽  
Neil C. Turner

In south-west Australia, winter grown crops such as wheat and lupin often experience transient waterlogging during periods of high rainfall. Wheat is believed to be more tolerant to waterlogging than lupins, but until now no direct comparisons have been made. The effects of waterlogging on root growth and anatomy were compared in wheat (Triticum aestivum L.), narrow-leafed lupin (Lupinus angustifolius L.) and yellow lupin (Lupinus luteus L.) using 1 m deep root observation chambers. Seven days of waterlogging stopped root growth in all species, except some nodal root development in wheat. Roots of both lupin species died back progressively from the tips while waterlogged. After draining the chambers, wheat root growth resumed in the apical region at a faster rate than well-drained plants, so that total root length was similar in waterlogged and well-drained plants at the end of the experiment. Root growth in yellow lupin resumed in the basal region, but was insufficient to compensate for root death during waterlogging. Narrow-leafed lupin roots did not recover; they continued to deteriorate. The survival and recovery of roots in response to waterlogging was related to anatomical features that influence internal oxygen deficiency and root hydraulic properties.


1967 ◽  
Vol 7 (26) ◽  
pp. 259 ◽  
Author(s):  
QA Shipton ◽  
CA Parker

Lime coating yellow lupin (Lupinus luteus L.) seed, when inoculated with bacteria from agar slopes, reduced nodulation substantially. By contrast, when peat cultures were used there was no adverse effect of lime coating on nodulation, even when inoculated seeds were stored for 61 days at 75�F, before planting. In two experiments the nodulation of unhulled serradella (Omithopus comljressus L.) was generally unaffected by lime coating when either agar or peat cultures were used. In a third experiment nodulation was significantly reduced with both agar and peat cultures on unhulled and hulled seed. This adverse effect was greater with agar cultures than with peat cultures. It is concluded that peat, and possibly the serradella seed pod, protects the bacteria from the effects of lime. The practice of lime coating lupin and serradella seed, when inoculated with peat cultures, is a useful technique, particularly when the seed is sown in contact with acid superphosphate. On the other hand, some adverse effects of lime on the nodulation of serradella when a peat culture was used suggest that other workers should test the suitability of the lime pelleting method under their own conditions. Rock phosphate and bentonite were also investigated as pelleting agents, but neither seemed to offer any advantages over lime.


2020 ◽  
Vol 21 (5) ◽  
pp. 1815
Author(s):  
Katarzyna Marciniak ◽  
Krzysztof Przedniczek

Precise control of generative organ development is of great importance for the productivity of crop plants, including legumes. Gibberellins (GAs) play a key role in the regulation of flowering, and fruit setting and development. The major repressors of GA signaling are DELLA proteins. In this paper, the full-length cDNA of LlDELLA1 gene in yellow lupine (Lupinus luteus L.) was identified. Nuclear-located LlDELLA1 was clustered in a second phylogenetic group. Further analyses revealed the presence of all conserved motifs and domains required for the GA-dependent interaction with Gibberellin Insensitive Dwarf1 (GID1) receptor, and involved in the repression function of LlDELLA1. Studies on expression profiles have shown that fluctuating LlDELLA1 transcript level favors proper flower and pod development. Accumulation of LlDELLA1 mRNA slightly decreases from the flower bud stage to anther opening (dehiscence), while there is rapid increase during pollination, fertilization, as well as pod setting and early development. LlDELLA1 expression is downregulated during late pod development. The linkage of LlDELLA1 activity with cellular and tissue localization of gibberellic acid (GA3) offers a broader insight into the functioning of the GA pathway, dependent on the organ and developmental stage. Our analyses provide information that may be valuable in improving the agronomic properties of yellow lupine.


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