Morphological Variability of Mangosteen in Bengkulu Province (Variabilitas Morfologi Manggis di Provinsi Bengkulu)

<p>Mangosteen is a genetic resource scattered and cultivated by farmers in Bengkulu Province. The study aimed at determining the morphological variability of mangosteen. The study was conducted from March to October 2015 in Bengkulu province using on-site supervision and observation methods. The number of plants observed was 34 genotypes and qualitatively and quantitatively characterized based on the Center for Plant Variety Protection and Agriculture Licensing (CPVPAL) guide. The data of morphology were analyzed by comparing the average value, variance, and standard deviation. Relationship or data grouping phenotypic characters appearance were identified using NTSYS software version 2.1. The results show wide variability in the density of leaf, flower size, and fruit weight, while narrow variability is occurred in plant height and the thickness of the rind. Data analysis of mangosteen genotypes generated similarity coefficients range from 0.71 to 0.91 (71-91%). Genotype 24 and 27 have the closest relationship, with the similarity coefficient of 91%.</p><p><strong>Keywords</strong></p><p>Mangosteen; Morphology; Variability; Character; Phenotypic</p><p><strong>Abstract</strong></p><p>Manggis merupakan sumber daya genetik yang tersebar dan dibudidayakan oleh petani di Provinsi Bengkulu. Penelitian bertujuan untuk mengetahui variabilitas fenotipik buah manggis. Penelitian dilaksanakan pada bulan Maret sampai Oktober 2015 di Provinsi Bengkulu dengan menggunakan metode observasi langsung morfologi tanaman manggis yang telah berbuah. Jumlah tanaman yang diamati sebanyak 34 genotipe dan masing-masing genotipe diamati karakterisasi kualitatif dan kuantitatif berdasarkan panduan Pusat Perlindungan Varietas Tanaman dan Perizinan Pertanian (PPVTPP). Analisis data melalui perbandingan varian fenotipik dengan standar deviasi varian fenotipe diukur dengan nilai rata-rata, varians, dan standar deviasi. Kekerabatan atau pengelompokan data penampilan karakter fenotipik dilakukan dengan menggunakan software NTSys versi 2.1. Hasil penelitian menunjukkan bahwa variabilitas luas terdapat pada kerapatan daun, ukuran bunga, dan bobot buah, sedangkan variabilitas sempit pada karakter tinggi tanaman dan ketebalan kulit buah. Analisis genotipe manggis menghasilkan koefisien kemiripan sebesar 0,71-0,91 (71-91%). Dari dendogram diperoleh genotipe yang memiliki tingkat kekerabatan yang tinggi terdapat pada genotipe 24 dan 27 dengan nilai kemiripan sebesar 91%.<strong></strong></p>

Aflatoxin Accumulation in a Maize Diallel Cross Containing Inbred Lines with Expired Plant Variety Protection

In-field infection of maize (Zea mays L.) ears by the fungus Aspergillus flavus Link:Fr causes pre-harvest aflatoxin contamination of maize grain. Germplasm lines with host-plant resistance to aflatoxin accumulation are available to breeders, but these lines often possess undesirable agronomic characteristics. Commercial lines with expired plant variety protection (ex-PVP lines) are a potential source of elite germplasm available to public maize breeders. A diallel cross containing three aflatoxin-accumulation-resistant germplasm lines and seven ex-PVP lines were evaluated in replicated trials for aflatoxin contamination after artificial inoculation and for yield. The resistant germplasm lines Mp313E, Mp715, and Mp717 were the only lines with significant general combining ability (GCA) for reduced aflatoxin accumulation. Of the ex-PVP lines evaluated, the Stiff-Stalk line F118 was the most promising line to use in breeding crosses. Based on its GCA, it was the only ex-PVP line that did not significantly increase aflatoxin and the only ex-PVP line that significantly increased yield. Second-cycle breeding lines derived from crosses between F118 and the resistant donor lines will be valuable if they combine the donor lines’ disease resistance with F118’s earlier maturity while introgressing the disease resistance into a genetic background that aligns with the industry’s well-defined heterotic groups.

Essentially derived varieties in the era of new breeding techniques

The current study sets out to analyse the concept of "essentially derived variety" (EDV) envisaged by the plant variety protection regime when new breeding techniques (NBTs) are employed in the development of new plant varieties. The use of NBTs to develop new plant varieties has grown rapidly over the last years because of their ease of use and their high efficiency. NBT varieties are mono-parental and retain most of the genome of the initial variety, thus most of its essential characteristics. The problem arises when the initial variety used as the source of genetic variation is a variety protected by a plant variety right. In this case, the question is whether the EDV concept should apply to the second variety obtained by NBTs and what can break the EDV chain. It must be noted that the EDV concept has revolutionised the plant variety protection system since it introduced the principle of "limited dependence" as an exception to the general independence principle governing this system. Therefore, as it is an exception, it should be interpreted rigorously.

Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection

Lavender species are widely distributed in their wild forms around the Mediterranean Basin and they are also cultivated worldwide as improved and registered clonal varieties. The economic interest of the species belonging to the Lavandula genus is determined by their use as ornamental plants and important source of essential oils that are destinated to the production of cosmetics, pharmaceuticals and foodstuffs. Because of the increasing number of cases of illegal commercialization of selected varieties, the protection of plant breeders’ rights has become of main relevance for the recognition of breeding companies’ royalties. With this aim, genomic tools based on molecular markers have been demonstrated to be very reliable and transferable among laboratories, and also much more informative than morphological descriptors. With the rising of the next-generation sequencing (NGS) technologies, several genotyping-by-sequencing approaches are now available. This study deals with a deep characterization of 15 varietal clones, belonging to two distinct Lavandula species, by means of restriction-site associated DNA sequencing (RAD-Seq). We demonstrated that this technology screens single nucleotide variants that enable to assess the genetic identity of individual accessions, to reconstruct genetic relationships among related breeding lines, to group them into genetically distinguishable main subclusters, and to assign their molecular lineages to distinct ancestors. Moreover, a number of polymorphic sites were identified within genes putatively involved in biosynthetic pathways related to both tissue pigmentation and terpene production, useful for breeding and/or protecting newly registered varieties. Overall, the results highlighted the presence of pure ancestries and interspecific hybrids for the analyzed Lavandula species, and demonstrated that RAD-Seq analysis is very informative and highly reliable for characterizing Lavandula clones and managing plant variety protection.

Begonia ’Crested‘: A new variety of Begonia from interspecific hybridization of Begonia sudjanae C.A Janson × Begonia puspitae Ardi

Improvement of leaf shape characters in Begonia can be done through hybridization between two different species of Begonia. This study aims to produce a new variety of Begonia to better improve the quantitative, qualitative characters and increase its genetic diversity. The study was conducted in the green house of Bogor Botanic Gardens. Two native species of Indonesia, Begonia sudjanae C.A Janson as the female parent and Begonia puspitae Ardi as the male parent. The mature F1 seeds were sown and selections were made among the plants produced. Observation results the characteristics of the new variety Begonia Crested as follows: stem type rhizomatous, leaves simple, ovate, peltate with strongly undulate base. Leaves margin is crenate and apex is acuminate. Colour on the upper surface are yellow green group RHS Colour Chart: (YG 144 A) strong yellow green, colour of veins on upper side is (YG 144 D) light yellow green. Inflorescence peduncle with 20 cm long branched symmetrically. The name Begonia Crested as the selected F1 plants are propagated in a vegetative method with leaves cutting, registered on the Center of Plant Variety Protection (PPVTPP) by No. 846/PVHP/2020. This new variety is beautiful and exotic leaves ornamental Begonia which will be developed as commercials ornamental plants.

Plant Variety Protection: Current Practices and Insights

Breeders persistently supply farmers with the best varieties in order to exceed consumer demand through plant-breeding processes that are resource-intensive. In order to motivate continuous innovation in variety development, a system needs to provide incentives for plant breeders to develop superior varieties, for example, exclusive ownership to produce and market those varieties. The most common system is the acquisition of intellectual property protection through plant variety protection, also known as the breeder’s right. Most countries have adopted the system established by the International Union for the Protection of New Varieties of Plants (UPOV). To be granted plant variety protection, the variety should prove to be unique by meeting three requirements: distinctness, uniformity, and stability. This review summarizes (1) the plant variety protection via UPOV convention, (2) technical methods for distinctness, uniformity, and stability testing via phenotype, molecular markers, and sequencing as well as their challenges and potentiality, and (3) additional discussions in essentially derived variety, value for cultivation and use testing, and open source seed initiative.

Law-Driven Innovation in Cereal Varieties: The Role of Plant Variety Protection and Seed Marketing Legislation in the European Union

The aim of this paper is to investigate the role of EU legislation in shaping innovation in cereal varieties. The research focuses on two fields of law and their relationship, i.e., intellectual property and agricultural law. More specifically, the normative legal investigation concerns the role played by Community plant variety protection and the EU legislation on the marketing of seed and plant propagating material in shaping innovation and stimulating plant breeding of new cereal varieties. The focus is on cereal varieties because innovation in this field has a great socio-economic impact, as well as strategic scientific and environmental implications. Breeding new cereal varieties is essential for the competitiveness of the seed and agricultural sector of the EU, and it can contribute to food security and the achievement of sustainable development goals. The study finds that it is necessary to simplify the existing legal framework by coordinating intellectual property and agricultural law, providing for legislative review and better coherence in order to effectively shape innovation and meet the changing demands of society and the sustainability challenges.

Heterotic Patterns of Temperate and Tropical Maize by Ear Photometry

As the plant variety protection (PVP) of commercial inbred lines expire, public breeding programs gain a wealth of genetic materials that have undergone many years of intense selection; however, the value of these inbred lines is only fully realized when they have been well characterized and are used in hybrid combinations. Additionally, while yield is the primary trait by which hybrids are evaluated, new phenotyping technologies, such as ear photometry (EP), may provide an assessment of yield components that can be scaled to breeding programs. The objective of this experiment was to use EP to describe the testcross performance of inbred lines from temperate and tropical origins. We evaluated the performance of 298 public and ex-PVP inbred lines and 274 Drought Tolerant Maize for Africa (DTMA) inbred lines when crossed to Iodent (PHP02) and/or Stiff Stalk (2FACC) testers for 25 yield-related traits. Kernel weight, kernels per ear, and grain yield predicted by EP were correlated with their reference traits with r = 0.49, r = 0.88, and r = 0.75, respectively. The testcross performance of each maize inbred line was tester dependent. When lines were crossed to a tester within the heterotic group, many yield components related to ear size and kernels per ear were significantly reduced, but kernel size was rarely impacted. Thus, the effect of heterosis was more noticeable on traits that increased kernels per ear rather than kernel size. Hybrids of DTMA inbred lines crossed to PHP02 exhibited phenotypes similar to testcrosses of Stiff Stalk and Non-Stiff Stalk heterotic groups for yield due to significant increases in kernel size to compensate for a reduction in kernels per ear. Kernels per ear and ear length were correlated (r = 0.89 and r = 0.84, respectively) with and more heritable than yield, suggesting these traits could be useful for inbred selection.

European Union Plant Variety Protection

This book explains how the Community plant variety rights system works and provides guidance regarding the field of law relating to the Basic Regulation and other implementing regulations. It gives an idea of how the grant system works, the advantages of Community plant variety rights, and the aspects to be considered in exploiting and defending. It also explains the mechanisms in the Basic Regulation on how infringements of Community plant variety rights should be dealt with, including certain enforcement systems of the EU Member States. This book analyses major aspects that are considered of practical relevance in infringement proceedings under the applicable national law. It elaborates how the case law is limited in comparison with patent infringement proceedings throughout the EU Member States.

Sowing and Cultivating the Seed of Diversity in Agri-Food: Intellectual Property Protection in Transnational and Comparative Perspective

This article combines intellectual property and comparative law insights to propose an evolutionary analysis of protection for seeds in the contemporary legal order. From a regime of commons for seeds, to plant variety protection, to patents for genetically engineered plants, exclusive rights have been progressively introduced for innovators in the field of agriculture to provide incentives in a world of growing need for food and raw materials. Such evolution has caused reduced common practices in agriculture that implied the freedom to save and exchange seeds. Among other values, a potential loss of biodiversity is the consequence. Likewise, legal systems tend to converge towards certain models of protection for innovators, although regulatory competition can exert significant pressure on states to preserve alternative models and offer solutions that balance all the values at stake.**