scholarly journals Erythrocyte index of residents exposed to lead in Tambaklorok, Semarang, Indonesia

2020 ◽  
Vol 9 (2) ◽  
pp. 151-158
Author(s):  
Budi Santosa ◽  
Herlisa Anggraini ◽  
Ika Dyah Mawarni ◽  
Niza Muliatun ◽  
Ardiansyah Nugroho ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Cross Sectional Study ◽  
P Value ◽  
Cross Sectional ◽  
Erythrocyte Morphology ◽  
Normal Category ◽  
Smear Preparation ◽  
Index Value

Erythrocyte index can form erythrocyte morphology in the peripheral blood smear preparation. Lead exposure in Tambaklorok exceeds the threshold that affects anemia. The purpose of this study was to determine the erythrocyte index confirmation with erythrocyte morphology in the peripheral blood smear preparation. A cross-sectional study was conducted in 2month in Tambaklorok Semarang residents and 104 samples were taken using the purposive technique. Erythrocyte index was measured using the hematological analyzer and erythrocyte morphology in the peripheral blood smear preparation using Giemsa painting. Erythrocyte index confirmation with the peripheral blood smear preparation was analyzed using the Gamma statistical relationship test. The results showed that the erythrocyte index value was mostly in the normal category, i.e., MCH 68 (64.4%), MCHC 61 (58.6%) and MCH 58 (56%) and below normal category were MCH 45 (42.95%), MCHC 41 (39.4%), and MCV 36 (34.3%). Erythrocyte morphology was mostly hypochromic, namely 46 (44.23%), normochrome 40 (38.46%), and hyperchrome 18 (17.3%). Relationship of MCH with erythrocyte color p-value 0.037 with size p-value 0.038. Conclusion of erythrocyte index confirmation, especially MCH with the peripheral blood smear preparation, there was a match on the color and size of erythrocytes, while the MCV and MCHC values had no significant relationship.

scholarly journals Assessment Of Peripheral Blood Smear Preparation Technique In Laboratories With High Sample Load

2017 ◽  
Vol 4 (4) ◽  
pp. A150-A156
Author(s):  
Sumanashree Mallappa ◽  
Sachin Kolte ◽  
Nimisha Sharma ◽  
Indrani Dhawan
Keyword(s):  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Preparation Technique ◽  
Smear Preparation

scholarly journals Erythrocyte Morphology in Women of Reproductive Age (WORA) with Anemia

Biomedika ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 83-91
Author(s):  
Alex Jekson Tukira ◽  
Lucia Sincu Gunawan ◽  
Edy Prasetya
Keyword(s):  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Reproductive Age ◽  
Target Cells ◽  
Women Of Reproductive Age ◽  
Erythrocyte Morphology ◽  
Smear Examination ◽  
Blood Smear Examination ◽  
Laboratory Technology

Anemia is a decrease in the number of erythrocytes in the blood circulation or the level of hemoglobin that is less than normal. The three major body mechanisms that cause anemia are excessive destruction of erythrocytes, blood loss, and decreased erythrocyte production. Based on the Basic Health Research (Riskesdas) in 2013, the prevalence of anemic women of reproductive age (WORA) aged 15-44 years in Indonesia was 35.3%. Anemia is classified based on the morphology of erythrocytes, including hypochromic microcytic, normocytic normochromic, and macrocytic. Erythrocyte morphology can be observed using peripheral blood smear examination. The objective of this study was to determine the morphology of erythrocytes in anemic women of reproductive age. This study belongs to descriptive research. The population of the study was 136 women of reproductive age, covering the students of D-IV in Medical Laboratory Technology at Setia Budi University. Forty-one respondents suffering from anemia were taken using a purposive sampling technique. The types of anemia were determined with examination using an Easy Touch hemoglobinometer with the Hb level of less than 12g/dL. Preparation of peripheral blood smear examination using EDTA venous blood and stained with Giemsa. Microscopic examination was performed with 1000x objective magnification. The peripheral blood smear reading showed the erythrocyte morphology that includes normocytic normochromic (38 samples or 93%), microcytic hypochromic (three samples or 7%), and poikilocytosis consisting of teardrop cells, target cells, ellipstocytes, and stomatocytes (five samples or 18%). Further study is required to investigate the correlation of erythrocyte index and peripheral blood smear in anemia.

scholarly journals Erythrocyte Morphology of Tuberculosis Patients

2019 ◽  
Vol 1 (1) ◽  
pp. 10-18
Author(s):  
Ahmad Rifa'i ◽  
Ahmad Muhlisin ◽  
Leka Lutpiatina
Keyword(s):  
Chronic Disease ◽  
Iron Deficiency ◽  
Chronic Diseases ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Deficiency Anemia ◽  
Morphological Description ◽  
Erythrocyte Morphology ◽  
Tuberculosis Patients

Tuberculosis is one of the causes of anemia in chronic diseases in addition to rheumatoid arthritis, urserative colitis, Crohn's disease, ovarian malignancy and Hodgkin's lymphoma. Chronic disease anemia also known as anemia due to inflammation, occurs in patients with chronic infections. This anemia also occurs in some patients with malignancy. The causes of anemia are based on cell size, namely iron deficiency (often), anemia of chronic diseases (often), Thalassemia (often in certain ethnicities) causing the size of cells to become microsytic. Cells turn macrocytic due to liver disease, excess alcohol, megaloblastic anemia (vitamin B12 deficiency or folate or exposure to certain drugs), hemolysis, and aplastic anemia. Whereas the normal cell factors are blood loss, the initial phase of iron deficiency, anemia, chronic disease, and kidney failure. The aim of this study was to determine the type of anemia based on the morphological description of erythrocytes in the form, color, and size of erythrocytes seen in the smear blood supply of tuberculosis patients based on treatment duration of 0-6 months. The type of research used was a descriptive survey with a population of 15 people taken by accidental sampling. Data collection is done by examining the blood smear smears. Based on the examination of peripheral blood smear preparations in pulmonary tuberculosis patients, the morphology of erythrocytes in the form of Normocytic normochrome was 74% and microsocytic normochrome was 26%. Suggestions in the research for further research can be carried out with support other than the examination of peripheral blood smear.

scholarly journals ASSOCIATION BETWEEN ATYPICAL DEPOLARIZATION IN CELLDYN 00 AND THE PRESENCE OF PLASMODIUM SPP IN BLOOD IN Dr. SOETOMO HOSPITAL SURABAYA

2015 ◽  
Vol 2 (1) ◽  
pp. 15
Author(s):  
Jusak Nugraha
Keyword(s):  
Peripheral Blood ◽  
Malaria Infection ◽  
Blood Smear ◽  
Screening Method ◽  
Peripheral Blood Smear ◽  
Accurate Method ◽  
High Morbidity ◽  
Parasitic Disease ◽  
Malaria Parasites ◽  
Cross Sectional

Background: Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate method is needed to detect the presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) in CBC results from Cell-Dyn 3200 has been related with malaria infection. Materials and Methods: An observational cross sectional approach with 48 samples obtained from inpatients of the Dr.Soetomo Hospital, Surabaya. Samples were screened by Cell-Dyn 3200 analyzer for atypdep flagging in CBC. Positive samples were later confirmed by microscope to detect malaria parasites. results: From 48 samples with atypdep flagging, 7 samples were malaria positive on peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignancy (18.7%), and approximately 54.6% of the samples were not accompanied by fever symptoms. Lekositosis and anemia each were found in 20 samples (41.6%) and thrombocytopenia in 33.3%. conclusion: The presence of atypdep flagging in Cell-Dyn 3200 does not necessarily indicate the existence of malaria or it could be said that atypdep flagging is not always associated with presence of malaria infection. The usage of an atypdep flagging in non-endemic areas such as Surabaya is just an alert sign to evaluate malaria infection rather than a screening method to detect malaria.

scholarly journals Anemia in chronic kidney disease patients in predialysis and postdialysis stages

1970 ◽  
Vol 1 (1) ◽  
pp. 26-29 ◽  
Author(s):  
S Bhatta ◽  
G Aryal ◽  
RK Kafle
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Serum Creatinine ◽  
Peripheral Blood ◽  
Peripheral Blood Smear ◽  
Cross Sectional Study ◽  
Moderate Degree ◽  
Cross Sectional ◽  
Significant Difference ◽  
Red Cell Indices

Background: Chronic kidney disease is usually associated with anemia and the level of anemia correlates with the severity of renal failure. This study was carried out to evaluate the profile of anemia and to find the correlation between the severity of anemia and serum creatinine levels in predialysis and postdialysis Chronic kidney disease patients. Materials and Methods: A cross-sectional study was conducted in 40 chronic kidney disease patients in the department of pathology and nephrology, KIST Medical College. Hemoglobin, hematocrit, red cell indices, peripheral blood smears and serum creatinine levels were examined using standard techniques. Results: There was a significant difference in the hemoglobin and hematocrit levels in predialysis and postdialysis patients (P <0.005). All 40 patients (100%) were anemic. Although moderate anemia was most common in both groups of patients, severe anemia was more common in postdialysis patients (5% in predialysis and 15% in postdialysis patients). The peripheral blood smear examinations showed that normocytic normochromic anemia was most frequent (90% in predialysis and 77.5 % in postdialysis patients). There was no significant correlation between the severity of anemia and serum creatinine levels (P > 0.05), r=-0.14 and -0.17 in predialysis and postdialysis group respectively.  Conclusion: The most frequent anemia in chronic kidney disease patients was normocytic and normochromic type of a moderate degree. Significant correlation was not found between the severity of anemia and serum creatinine levels in both pre and postdialysis group of patients. Keywords: Chronic Kidney Disease; Anemia; Creatinine; Hemoglobin; Hematocrit; Hemodialysis DOI: 10.3126/jpn.v1i1.4446 Journal of Pathology of Nepal (2011) Vol.1, 26-29

scholarly journals Etiological and Clinical Spectrum of Pancytopenia Based on Peripheral Blood Smear and Bone Marrow Examination: A Cross-sectional Study

2021 ◽  
pp. 26-32
Author(s):  
Vipin Porwal ◽  
Rajesh Deshpande ◽  
Rohit Modi
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
General Medicine ◽  
Peripheral Blood Smear ◽  
Bone Marrow Examination ◽  
Cross Sectional Study ◽  
Megaloblastic Anaemia ◽  
Bleeding Tendency ◽  
Cross Sectional ◽  
Common Clinical Finding

Aims: Pancytopenia is a common clinic-haematological problem suspected in patients with anaemia, prolonged fever, and a bleeding tendency.  This study was performed to find the prevalence of pancytopenia and to determine the common causes of pancytopenia. Study Design:  Cross-sectional observational study. Place and Duration of Study: department of general medicine at R. D. Gardi Medical College, Ujjain, India between November 2017 toAugust 2019. Methodology: The study was conducted among patients with pancytopenia during a two-year period. The etiological pattern was assessed through routine blood tests to determine their clinical features, peripheral blood pictures, and bone marrow morphologies. Results: Out of 100 patients with pancytopenia, the majority (64.0%) were men. A total of 34 patients were aged between the 21 and 30 years and 28 were aged between 31 and 40 years. Generalized weakness was the most common (88%) presentation and the most common clinical finding was pallor (94.0%), followed by splenomegaly (40.0%) and hepatomegaly (30.0%). Megaloblastic anaemia was the most common cause of pancytopenia that was observed in 58 patients, followed by aplastic anaemia (n=12), cirrhosis of the liver (n=8), leukaemia (n=6), dengue, myelodysplastic syndrome, and malaria (n=4 each), paroxysmal nocturnal haemoglobinuria and acquired immunodeficiency syndrome (n=2 each). A total of 28.0% patients had normocellular bone marrow and 72.0% had cellular marrow. Conclusion: Megaloblastic anaemia was the most common aetiology of pancytopenia.

scholarly journals HUBUNGAN ANTARA FLAGGING ATYPDEP DI ALAT CELL-DYN 3200 DAN KEBERADAAN PLASMODIUM Spp DI DALAM DARAH PENDERITA DI RSUD DR.SOETOMO SURABAYA

2018 ◽  
Vol 17 (2) ◽  
pp. 97
Author(s):  
Esti Rohani ◽  
J Nugraha
Keyword(s):  
Peripheral Blood ◽  
Malaria Infection ◽  
Blood Smear ◽  
Malignant Disease ◽  
Screening Method ◽  
Peripheral Blood Smear ◽  
High Morbidity ◽  
Parasitic Disease ◽  
Malaria Parasites ◽  
Cross Sectional

Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate methods is needed to detectthe presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) on CBC result from Cell-Dyn 3200instrument has been related with malaria infection. An observational cross sectional approach with a total of 48 samples were obtainedfrom inpatients in the Dr. Soetomo Hospital Surabaya. Samples were screened with Cell-Dyn 3200 analyzer for CBC found atypdepflagging. The positive samples were later confirmed by microscopic to detect malaria parasites. From 48 samples with atypdep flagging,seven samples were positive of malaria in peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignant disease(18.7), an approximately 54.6% of the sample is not accompanied by symptoms of fever. Lekositosis and anemia were found in each of20 samples (41.6%) and thrombocytopenia in 33.3% of the samples. The presence of atypdep flagging does not necessarily indicate theexistence of malaria infection or it could be said that atypdep flagging is not always associated with the presence of malaria infection.The usage of an atypdep flagging on Cell-Dyn instrument in non-endemic areas such as Surabaya is just an alert sign to evaluate themalaria infection rather than a screening method to detect malaria.

Bone Marrow and Peripheral Blood Evaluation in Non-Hodgkin Lymphoma – A Cross Sectional Study of 78 Cases in a Tertiary Care Centre in Kerala

2021 ◽  
Vol 8 (32) ◽  
pp. 2943-2949
Author(s):  
Arya Puthukkat Muraleedharan ◽  
Prabhalakshmy Kuzhikkattil Krishnankutty
Keyword(s):  
Bone Marrow ◽  
Hodgkin Lymphoma ◽  
Peripheral Blood ◽  
Bone Marrow Aspirate ◽  
Peripheral Blood Smear ◽  
Cross Sectional Study ◽  
Lymphoid Cells ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma ◽  
Blood Smears

BACKGROUND In the evaluation of patients with non-Hodgkin lymphoma (NHL), determination of bone marrow involvement is an integral part of staging work up. Peripheral blood counts and examination of blood smears are also done in patients with lymphoma as part of pre-treatment investigations. METHODS A cross sectional study of 78 patients with a prior histopathological diagnosis of NHL was conducted. Peripheral blood counts were performed on an automated haematology analyser to look for various cytopenias. Peripheral blood smears and bone marrow aspirate (BMA) / imprint smears were examined in detail for atypical lymphoid cells. Bone marrow trephine biopsies of these patients were studied to assess the NHL involvement and the various patterns of involvement. Adjuvant immunohistochemistry (IHC) was performed in bone marrow biopsies with scant cellularity or crush artefact to discern the marrow involvement. RESULTS Bone marrow trephine biopsy showed involvement by lymphoma in 65.4 % cases. The incidence of involvement was higher in B-cell lymphomas, especially in low grade types. The predominant pattern of involvement was interstitial pattern (41.2 %). Discordant histology between bone marrow and the primary anatomic site was found in 7.8 % of the cases, which was seen more in diffuse large B-cell lymphomas. Majority of the patients with bone marrow infiltration by NHL had anaemia (84.3 %). Bicytopenia and pancytopenia were also observed. On peripheral blood smear examination atypical lymphoid cells were present in 23 % cases. CONCLUSIONS Bone marrow examination is an important aspect in the diagnosis of NHL, because of its both prognostic and therapeutic implications. Hence, the presence of atypical lymphoid cells and other changes in the peripheral blood should be detected in these patients. KEYWORDS Non-Hodgkin Lymphoma, Bone Marrow Biopsy, Bone Marrow Aspirate / Imprint, Peripheral Blood Smear, Atypical Lymphoid Cells

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