Bone Marrow and Peripheral Blood Evaluation in Non-Hodgkin Lymphoma – A Cross Sectional Study of 78 Cases in a Tertiary Care Centre in Kerala

2021 ◽  
Vol 8 (32) ◽  
pp. 2943-2949
Author(s):  
Arya Puthukkat Muraleedharan ◽  
Prabhalakshmy Kuzhikkattil Krishnankutty
Keyword(s):  
Bone Marrow ◽  
Hodgkin Lymphoma ◽  
Peripheral Blood ◽  
Bone Marrow Aspirate ◽  
Peripheral Blood Smear ◽  
Cross Sectional Study ◽  
Lymphoid Cells ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma ◽  
Blood Smears

BACKGROUND In the evaluation of patients with non-Hodgkin lymphoma (NHL), determination of bone marrow involvement is an integral part of staging work up. Peripheral blood counts and examination of blood smears are also done in patients with lymphoma as part of pre-treatment investigations. METHODS A cross sectional study of 78 patients with a prior histopathological diagnosis of NHL was conducted. Peripheral blood counts were performed on an automated haematology analyser to look for various cytopenias. Peripheral blood smears and bone marrow aspirate (BMA) / imprint smears were examined in detail for atypical lymphoid cells. Bone marrow trephine biopsies of these patients were studied to assess the NHL involvement and the various patterns of involvement. Adjuvant immunohistochemistry (IHC) was performed in bone marrow biopsies with scant cellularity or crush artefact to discern the marrow involvement. RESULTS Bone marrow trephine biopsy showed involvement by lymphoma in 65.4 % cases. The incidence of involvement was higher in B-cell lymphomas, especially in low grade types. The predominant pattern of involvement was interstitial pattern (41.2 %). Discordant histology between bone marrow and the primary anatomic site was found in 7.8 % of the cases, which was seen more in diffuse large B-cell lymphomas. Majority of the patients with bone marrow infiltration by NHL had anaemia (84.3 %). Bicytopenia and pancytopenia were also observed. On peripheral blood smear examination atypical lymphoid cells were present in 23 % cases. CONCLUSIONS Bone marrow examination is an important aspect in the diagnosis of NHL, because of its both prognostic and therapeutic implications. Hence, the presence of atypical lymphoid cells and other changes in the peripheral blood should be detected in these patients. KEYWORDS Non-Hodgkin Lymphoma, Bone Marrow Biopsy, Bone Marrow Aspirate / Imprint, Peripheral Blood Smear, Atypical Lymphoid Cells

scholarly journals Etiological and Clinical Spectrum of Pancytopenia Based on Peripheral Blood Smear and Bone Marrow Examination: A Cross-sectional Study

2021 ◽  
pp. 26-32
Author(s):  
Vipin Porwal ◽  
Rajesh Deshpande ◽  
Rohit Modi
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
General Medicine ◽  
Peripheral Blood Smear ◽  
Bone Marrow Examination ◽  
Cross Sectional Study ◽  
Megaloblastic Anaemia ◽  
Bleeding Tendency ◽  
Cross Sectional ◽  
Common Clinical Finding

Aims: Pancytopenia is a common clinic-haematological problem suspected in patients with anaemia, prolonged fever, and a bleeding tendency.  This study was performed to find the prevalence of pancytopenia and to determine the common causes of pancytopenia. Study Design:  Cross-sectional observational study. Place and Duration of Study: department of general medicine at R. D. Gardi Medical College, Ujjain, India between November 2017 toAugust 2019. Methodology: The study was conducted among patients with pancytopenia during a two-year period. The etiological pattern was assessed through routine blood tests to determine their clinical features, peripheral blood pictures, and bone marrow morphologies. Results: Out of 100 patients with pancytopenia, the majority (64.0%) were men. A total of 34 patients were aged between the 21 and 30 years and 28 were aged between 31 and 40 years. Generalized weakness was the most common (88%) presentation and the most common clinical finding was pallor (94.0%), followed by splenomegaly (40.0%) and hepatomegaly (30.0%). Megaloblastic anaemia was the most common cause of pancytopenia that was observed in 58 patients, followed by aplastic anaemia (n=12), cirrhosis of the liver (n=8), leukaemia (n=6), dengue, myelodysplastic syndrome, and malaria (n=4 each), paroxysmal nocturnal haemoglobinuria and acquired immunodeficiency syndrome (n=2 each). A total of 28.0% patients had normocellular bone marrow and 72.0% had cellular marrow. Conclusion: Megaloblastic anaemia was the most common aetiology of pancytopenia.

STUDY OF HISTOPATHOLOGY IN PATIENTS WITH HODGKIN AND NON - HODGKIN LYMPHOMA

2016 ◽  
pp. 59-65 ◽  
Author(s):  
Van Mao Nguyen
Keyword(s):  
Lymph Node ◽  
Hodgkin Lymphoma ◽  
Cross Sectional Study ◽  
University Hospital ◽  
Cross Sectional ◽  
Histopathological Classification ◽  
Non Hodgkin Lymphoma ◽  
Key Words Lymphoma ◽  
Malignant Grade

Background: Lymphoma is one of the most ten common cancers in the world as well as in Vietnam which has been ever increasing. It was divided into 2 main groups Hodgkin and non – Hodgkin lymphoma in which non-Hodgkin lymphoma appeared more frequency, worse prognosis and different therapy. Objectives: - To describe some common characteristics in patients with non – Hodgkin lymphoma; - To determine the proportion between Hodgkin and non- Hodgkin lymphoma, histopathological classification of classical Hodgkin by modified Rye 1966 and non-Hodgkin lymphoma by Working Formulation (WF) of US national oncology institute 1982. Materials and Method: This cross-sectional study was conducted on 65 patients with Hodgkin and non- Hodgkin lymphoma diagnosed definitely by histopathology at Hue Central Hospital and Hue University Hospital. Results:. The ratio of male/female for the non-Hodgkin lymphoma was 1.14/1, the most frequent range of age was 51-60 accounting for 35%, not common under 40 years. Non - Hodgkin lymphoma appeared at lymph node was the most common (51.7%), at the extranodal site was rather high 48.3%. The non - Hodgkin lymphoma proportion was predominant 92.3% comparing to the Hodgkin lymphoma only 7.7%; The most WF type was WF7 (53.3%), following the WF6 18,3% and WF5 11,7%; The intermediate malignancy grade of non- Hodgkin lymphoma was the highest proportion accouting for 85%, then the low and the high one 8.3% and 6.7% respectively. Conclusion: The histopathological classification and the malignant grade of lymphoma for Hodgkin and non - Hodgkin lymphoma played a practical role for the prognosis and the treatment orientation, also a fundamental one for the modern classification of non - Hodgkin lymphoma nowadays. Key words: lymphoma, Hodgkin lymphoma, non-Hodgkin lymphoma, classication, grade, histopathology, lymph node

scholarly journals Long‐term fatigue in survivors of non‐Hodgkin lymphoma: The Lymphoma Study Association SIMONAL cross‐sectional study

Cancer ◽  
2019 ◽  
Author(s):  
Nicolas Mounier ◽  
Sabine Anthony ◽  
Raphaël Busson ◽  
Catherine Thieblemont ◽  
Vincent Ribrag ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma ◽  
Study Association

scholarly journals Comprehensive Geriatric Assessment in Elderly Patients with Aggressive Non-Hodgkin Lymphoma in Peru

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 2307-2307
Author(s):  
Brady E. Beltrán ◽  
Rodrigo Motta ◽  
María P. Gamarra ◽  
Roger Araujo ◽  
Jorge J. Castillo
Keyword(s):  
Hodgkin Lymphoma ◽  
Geriatric Assessment ◽  
Comprehensive Geriatric Assessment ◽  
Research Funding ◽  
Cross Sectional Study ◽  
Bivariate Analysis ◽  
Geriatric Syndrome ◽  
Geriatric Syndromes ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma

Abstract Introduction: The incidence of Non-Hodgkin Lymphoma (NHL) is increasing, especially in people over 60 years of age. This population usually has a worse prognosis, probably due to comorbidities, functional deterioration and decreased tolerance to treatment. Therefore, a pre-therapeutic evaluation would be important to make decisions. The Comprehensive Geriatric Assessment (CGA) is the recommended tool for this evaluation, but it is a complex process that demands time and resources. We performed a study to evaluate the characteristics of the CGA in patients >64 years of age with NHL and to determine which domains can constitute a simplified model. Methods: This is a cross-sectional study with retrospective data collection of geriatric evaluations performed in >64 years with aggressive NHL (90% DLBCL, 10% PTCL) admitted to our institution between September 2015 and August 2017. Number of drugs, prescriptions in older adults was evaluated (STOPP), Lawton scale, Barthel scale, KATZ index, walking speed, Up and Go time (TUG), Mini-mental test, Yesavage scale, Gijón scale, Mini-nutritional, Geriatric syndromes (Incontinence, Falls, Pressure ulcers, Immobility, Sensory Deficits, Osteoporosis), and Accumulated Disease Scale in Geriatrics (CIRS-G). The CGA included 9 domains, and fragility was defined as deterioration in >2 domains. The different evaluations were compared with fragility. Multivariate models were constructed using logistic regression. Results: We included 253 patients with an average age of 76 years. 62% had >1 affected domain, and 40% were considered fragile (>2 affected domains). In the bivariate analysis, age >85 years, and all the geriatric scales except STOPP, were strongly associated with fragility. The final model had 6 variables: Use of >5 drugs ( OR 773.1, 95% CI,4.5-132134.4;p=0.011), Lawton scale <7 (OR 385.2,95%CI, 20.1-7363.5;p<0.001), TUG >20 (OR 124.6,95%CI, 6.2-2489.8;p=0.002), Mini-mental <23 (OR 53.5,95%CI, 3.7-778.8;p=0.004),Yesavage scale >5 (OR 118.7,95%CI, 11.2-1254.5;p<0.001), and presence of at least one geriatric syndrome (OR 235.6,95%CI, 13.3-4158.7;p<0.001). Removing the Mini-mental minimally affected the model, but suppressing two or more variables does weaken the model. Conclusions: In our cohort of patients older than 64 years with aggressive NHL, a model based on five measurements (i.e. number of drugs, TUG, Lawton scale, Yesavage scale and Geriatric Syndromes) could constitute an evaluation with an efficiency similar to a complete CGA. Disclosures Castillo: Pharmacyclics: Consultancy, Research Funding; Genentech: Consultancy; Millennium: Research Funding; Abbvie: Consultancy, Research Funding; Janssen: Consultancy, Research Funding; Beigene: Consultancy, Research Funding.

Economic burden in non‐Hodgkin lymphoma survivors: The French Lymphoma Study Association SIMONAL cross‐sectional study

Cancer ◽  
2021 ◽  
Author(s):  
Virginie Nerich ◽  
Christophe Guyeux ◽  
Michel Henry‐Amar ◽  
Raphaël Couturier ◽  
Catherine Thieblemont ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Economic Burden ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Non Hodgkin Lymphoma ◽  
Study Association ◽  
Lymphoma Survivors

scholarly journals A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Alice Charwudzi ◽  
Edeghonghon E. Olayemi ◽  
Ivy Ekem ◽  
Olufunmilayo Olopade ◽  
Mariann Coyle ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Molecular Diagnosis ◽  
Peripheral Blood ◽  
Peripheral Blood Smear ◽  
Full Blood Count ◽  
Interphase Fish ◽  
Genetic Abnormalities ◽  
Blood Smears ◽  
Peripheral Blood Smears ◽  
Cytogenetic Technique

Background.FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow and peripheral blood smear slides transported to a more equipped facility for molecular diagnosis of CML.Methods.Interphase FISH was performed on 22 archival methanol-fixed marrow (BM) and 3 peripheral blood (PB) smear slides obtained at diagnosis. The BM smears included 20 CML and 2 CMML cases diagnosed by morphology; the 3 PB smears were from 3 of the CML patients at the time of diagnosis. Six cases had knownBCR-ABLfusion results at diagnosis by RQ-PCR. Full blood count reports at diagnosis were also retrieved.Result.19 (95%) of the CML marrow smears demonstrated theBCR-ABLtranslocation. There was a significant correlation between theBCR-ABLtranscript detected at diagnosis by RQ-PCR and that retrospectively detected by FISH from the aged BM smears at diagnosis (r=0.870;P=0.035).Conclusion.Archival methanol-fixed marrow and peripheral blood smears can be used to detect theBCR-ABLtranscript for CML diagnosis.

scholarly journals Anemia in chronic kidney disease patients in predialysis and postdialysis stages

1970 ◽  
Vol 1 (1) ◽  
pp. 26-29 ◽  
Author(s):  
S Bhatta ◽  
G Aryal ◽  
RK Kafle
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Serum Creatinine ◽  
Peripheral Blood ◽  
Peripheral Blood Smear ◽  
Cross Sectional Study ◽  
Moderate Degree ◽  
Cross Sectional ◽  
Significant Difference ◽  
Red Cell Indices

Background: Chronic kidney disease is usually associated with anemia and the level of anemia correlates with the severity of renal failure. This study was carried out to evaluate the profile of anemia and to find the correlation between the severity of anemia and serum creatinine levels in predialysis and postdialysis Chronic kidney disease patients. Materials and Methods: A cross-sectional study was conducted in 40 chronic kidney disease patients in the department of pathology and nephrology, KIST Medical College. Hemoglobin, hematocrit, red cell indices, peripheral blood smears and serum creatinine levels were examined using standard techniques. Results: There was a significant difference in the hemoglobin and hematocrit levels in predialysis and postdialysis patients (P <0.005). All 40 patients (100%) were anemic. Although moderate anemia was most common in both groups of patients, severe anemia was more common in postdialysis patients (5% in predialysis and 15% in postdialysis patients). The peripheral blood smear examinations showed that normocytic normochromic anemia was most frequent (90% in predialysis and 77.5 % in postdialysis patients). There was no significant correlation between the severity of anemia and serum creatinine levels (P > 0.05), r=-0.14 and -0.17 in predialysis and postdialysis group respectively.  Conclusion: The most frequent anemia in chronic kidney disease patients was normocytic and normochromic type of a moderate degree. Significant correlation was not found between the severity of anemia and serum creatinine levels in both pre and postdialysis group of patients. Keywords: Chronic Kidney Disease; Anemia; Creatinine; Hemoglobin; Hematocrit; Hemodialysis DOI: 10.3126/jpn.v1i1.4446 Journal of Pathology of Nepal (2011) Vol.1, 26-29

Immunophenotyping of mature B-cell non Hodgkin lymphoma involving bone marrow and peripheral blood: critical analysis and insights gained at a tertiary care cancer hospital

2009 ◽  
Vol 50 (8) ◽  
pp. 1290-1300 ◽  
Author(s):  
Sumeet Gujral ◽  
Sunita Narayan Polampalli ◽  
Y. Badrinath ◽  
Ashok Kumar ◽  
Subramanian P. G. ◽  
...  
Keyword(s):  
Bone Marrow ◽  
B Cell ◽  
Hodgkin Lymphoma ◽  
Peripheral Blood ◽  
Critical Analysis ◽  
Tertiary Care ◽  
Non Hodgkin Lymphoma ◽  
Cancer Hospital
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