scholarly journals Studi Histokimia Sebaran Karbohidrat Usus Biawak Air (Varanus salvator)

2016 ◽  
Vol 3 (2) ◽  
pp. 77-84 ◽  
Author(s):  
Sri Wahyuni ◽  
. Zuchri ◽  
. Hamny ◽  
Muhammad Jalaluddin ◽  
I Ketut Mudite Adnyane
Keyword(s):  
Alcian Blue ◽  
Histochemical Study ◽  
Periodic Acid ◽  
Goblet Cells ◽  
Staining Reaction ◽  
Intestinal Tissue ◽  
Periodic Acid Schiff ◽  
Weak Reaction ◽  
Intense Staining ◽  
Varanus Salvator

Penelitian ini bertujuan mengetahui sebaran karbohidrat pada usus biawak air dengan metode histokimia. Organ usus dari satu ekor biawak jantan dewasa dikoleksi melalui prosedur perfusi dan selanjutnya difiksasi dengan larutan paraformaldehid 4%. Usus biawak dibagi menjadi enam bagian, yaitu bagian I sampai VI dan selanjutnya diproses menjadi preparat histologi. Deteksi sebaran karbohidrat pada lapisan mukosa usus dilakukan dengan pewarnaan alcian blue (AB) pH 2,5 untuk karbohidrat asam dan periodic acid schiff (PAS) untuk karbohidrat netral. Hasil penelitian menunjukan bahwa sebaran karbohidrat asam ditemukan pada sel goblet pada keenam bagian usus dengan intensitas reaksi sedang (++) sampai kuat (+++), dan tidak ditemukan pada struktur usus lainnya. Sebaran karbohidrat netral ditemukan di seluruh permukaan jaringan usus dengan intensitas reaksi lemah (+) sedangkan intensitas reaksi sedang (++) sampai kuat (+++) ditemukan pada sel goblet. Jumlah sel goblet penghasil karbohidrat asam dan netral pada usus bagian I-IV lebih sedikit (+~++) dibandingkan usus bagian V-VI (+++). Berdasarkan hasil penelitian dapat disimpulkan bahwa sebaran karbohidrat asam dan netral ditemukan diseluruh bagian usus dengan intensitas pewarnaan yang bervariasi. Jumlah sel goblet yang terdeteksi menghasilkan kedua jenis karbohidrat tersebut lebih banyak ditemukan pada usus bagian kaudal.Kata kunci: jaringan usus, sel goblet, karbohidrat asam dan netral, Varanus salvator. (Histochemical Study of Intestinal Carbohydrates Distribution in the Water Monitor (Varanus salvator))The objective of this study was to elaborate the distribution of carbohydrate in intestine tissue of water monitor using histochemical method. Intestinal organ from an adult male water monitor was collected after perfused and subsequently fixed in paraformaldehyde 4%. Intestinal organ was divided to six regions e.g. I to VI and then processed to histological slides. The carbohydrate distribution on the mucosal surface of intestinal tissue was stained with alcian blue (AB) pH 2.5 for detect the acid carbohydrates and periodic acid Schiff (PAS) for the neutral carbohydrates. The results showed that the distribution of acid carbohydrates found in the goblet cells at the I to VI regions with vary intensity of staining reaction with good staining (++) to intense staining (+++), whereas other intestinal structures did not contain the acid carbohydrates. Furthermore, the distribution of neutral carbohydrates was found in the whole intestinal tissue with weak reaction (+), while good staining (++) to intense staining (+++) was appeared in the goblet cells. Additionally, the number of goblet cells containing acid and neutral carbohydrates at the I-IV region was fewer (+~++) than at V-VI region (+++). Conclusion of this study is the distribution of acid and neutral carbohydrates appeared in all regions of the intestinal tissue. The large number of goblet cells that secreting both of carbohydrate type was found at the caudal of intestinal tissue.Keywords: intestinal tissue, goblet cells, acid and neutral carbohydrates, Varanus salvator.

Agarose plug instillation causes goblet cell metaplasia by activating EGF receptors in rat airways

2000 ◽  
Vol 278 (1) ◽  
pp. L185-L192 ◽  
Author(s):  
Heung-Man Lee ◽  
Kiyoshi Takeyama ◽  
Karim Dabbagh ◽  
James A. Lausier ◽  
Iris F. Ueki ◽  
...  
Keyword(s):  
Alcian Blue ◽  
Goblet Cell ◽  
Kinase Inhibitor ◽  
Periodic Acid ◽  
Goblet Cells ◽  
Secretory Cells ◽  
Egf Receptors ◽  
Periodic Acid Schiff ◽  
Egfr Expression ◽  
Goblet Cell Metaplasia

We hypothesized that foreign bodies in airways cause inflammation leading to goblet cell metaplasia. Instilled agarose plugs lodged in the bronchi of pathogen-free rats caused a time-dependent increase in Alcian blue-periodic acid-Schiff staining that was detected within 24 h and markedly increased at 72 h. Control bronchi contained no pregoblet or goblet cells, but plugged bronchi contained many pregoblet and goblet cells and a decrease in nongranulated secretory cells. In situ hybridization showed no expression of MUC5AC in control airways, but plugged airways showed a marked expression. Control bronchi showed sparse staining for epidermal growth factor receptor (EGFR) protein, but plugged bronchi showed intense EGFR staining in the epithelium. Pretreatment with an EGFR tyrosine kinase inhibitor (BIBX1522) prevented Alcian blue-periodic acid-Schiff staining and MUC5AC gene expression in plugged bronchi. Pretreatment with tumor necrosis factor-α neutralizing antibody or pretreatment with cyclophosphamide abolished plug-induced EGFR protein expression and goblet cell metaplasia. Thus instillation of agarose plugs induces profound goblet cell metaplasia by causing EGFR expression and activation.

scholarly journals Light microscopic study on the absorptive cells and goblet cells in the intestine of adult common carp Cyprinus carpio

2019 ◽  
Vol 43 (1) ◽  
pp. 148-155
Author(s):  
Khalid Hadi Kadhim
Keyword(s):  
Alcian Blue ◽  
Common Carp ◽  
Periodic Acid ◽  
Goblet Cells ◽  
Staining Intensity ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Posterior Portion ◽  
Absorptive Cells ◽  
Mucosal Folds

     The purpose of this study was to describe of some histological structures and histochemical features of the absorptive cells and goblet cells in intestine of common carp. In this study, Fifteen adult male common carp were catching alive from the AL-Forat river, with age about (7 - 12) months and mean of their weight was (2100 ± 81 g) and mean standard length was (50.4 ± 3.1 cm), immediately after death.Incision was made through the midventral line of the fish just from cranial to the anus to expose the intestine, specimens of intestine were taken and washed with (0.9 %) normal saline solution. Ten samples were obtained from different regions of each portion of the intestine (anterior, middle and posterior), fixed by either 10% neutral buffered formalin or Bouin’s solution approximately 24 hours at room temperature and then treated by routine histological processing. The stains were used, Hematoxylin and Eosin, periodic acid Schiff, Alcian blue pH 2.5, and Combinated Alcian blue PH 2.5 plus periodic acid Schiff. The Mean number, height and width of mucosal folds were measured and counts of goblet cells in the mucosal folds of each portion of the intestine. The results showed that the intestinal epithelium is simple columnar, the enterocytes were tall columnar cells with brush border; goblet cells were ovoid, located between the enterocytes. The folds composed of the entire thickness of the mucosa,  mean number, height and width of the mucosal folds were few in posterior portion of intestine compare with that in other portions. The anterior portion of the intestine has largest number of mucosal folds, while has less number of goblet cells compare to middle and posterior portions of the intestine. The epithelial cells stained red-purple by periodic acid Schiff stain with Alcian blue, the goblet cells were take positive reaction with periodic acid Schiff stain, and with Alcian blue plus periodic acid Schiff, while negative with only Alcian blue stain. Glycoprotein staining intensity varied from very weak to moderate, whereby the presence of neutral, acidic and sulphatedglycoconjugates. In conclusion, numbers of goblet cells were observed to be increased towards posterior portion of intestine required as lubricant for fecal expulsion, and reversed relation between number of the folds and goblet cells.

scholarly journals Histological and Histochemical studies of Esophagus in Mabuya aurata septemaeniata

2015 ◽  
Vol 12 (3) ◽  
pp. 437-444
Author(s):  
Baghdad Science Journal
Keyword(s):  
Epithelial Cells ◽  
Alcian Blue ◽  
Periodic Acid ◽  
Goblet Cells ◽  
Muscularis Mucosa ◽  
Periodic Acid Schiff

The wall of the esophagus in Mabuya aurata septemaeniata as in high vertebrates consists of four layers, mucosa, submucosa,muscularis and serosa. Mucosa forms many unorganized short and long folds penetrate inside the esophageal cavity. Mucosa contains two sub layers, first one is lining epithelium which includes two types of cells, simple ciliated columnar epithelial cells and goblet cells, second one is lamina properia. Mucosa does not have muscularis mucosa. There is no esophageal glands within esophagus. Many special stains were used as (Periodic Acid Schiff (PAS)) to detect Carbohydrates in goblet cells. Alcian blue were used to detect the amount of goblet cells within lining epithelium. Alcian blue + PAS together confirm that the secreted mucin from goblet cells was acidic.

Distributions of Eustachian Tube Goblet Cells and Glands in Children with and without Otitis Media

1992 ◽  
Vol 101 (9) ◽  
pp. 750-754 ◽  
Author(s):  
Shoji Matsune ◽  
Isamu Sando ◽  
Haruo Takahashi
Keyword(s):  
Light Microscopy ◽  
Temporal Bone ◽  
Otitis Media ◽  
Alcian Blue ◽  
Eustachian Tube ◽  
Periodic Acid ◽  
Goblet Cells ◽  
Periodic Acid Schiff ◽  
Submucosal Glands

The distributions of the goblet cells and submucosal glands in eustachian tubes (ETs) from children with or without otitis media (OM) were studied. From 12 temporal bone—ET specimens obtained from children 1 to 7 years old at death, representative vertically cut histology sections were selected, stained with periodic acid—Schiff–Alcian blue and hematoxylin, and studied by light microscopy. In children without OM, the density of the goblet cells was significantly lower in the roof than in the floor of the ET and was highest in the midcartilaginous portion; glands were most richly distributed near the pharyngeal orifice, and became less densely distributed toward the tympanic portion. In children with OM, the density of the goblet cells was significantly higher both in the roof and floor portions, and the glands were more richly distributed than in the ETs of children without OM. The possibility that the observed increase in ET secretory components in children with OM impairs the passage of air through these tubes is discussed.

scholarly journals Comparative features of the mucous glands in the gastroduodenal junction in guinea pigs and chinchillas: microanatomy and histochemistry

2019 ◽  
Author(s):  
Adrian Florin Gal ◽  
Vasile Rus ◽  
Sanda Andrei ◽  
Viorel Miclaus
Keyword(s):  
Alcian Blue ◽  
Guinea Pigs ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Weak Reaction ◽  
Tissue Samples ◽  
Glandular Cells ◽  
Blue Stain ◽  
Acid Mucosubstances ◽  
Goldner’S Trichrome

Abstract Background: Mucous glands from the gastroduodenal junction are briefly represented by glandular cells in the stomach and by Brunner’s glands in the duodenum. The aim of the study was to describe the main histological/histochemical features of the gastroduodenal junction in guinea pigs and chinchillas. Results: The material was represented by tissue samples collected from the stomach and duodenum processed by paraffin technique. Histological sections were stained by Goldner’s trichrome stain, while mucous content was identified by Periodic acid–Schiff (PAS) stain (for neutral mucosubstances) and Alcian blue stain (for acid mucosubstances). In guinea pig, the pyloric glands were positive for both mucus types, whereas in chinchillas a low amount of mucin was identified. The Bruner glands, in guinea pigs displayed a weak reaction for the both mucin types. In chinchillas, the Bruner glands showed a significantly higher amount of PAS and Alcian blue-positives mucosubstances types. As observed, a higher amount of neutral mucus was identified in the pyloric glands in guinea pigs comparing to chinchillas. Contrariwise, chinchillas displayed a suggestively higher amount of mucus in Bruner glands comparing to guinea pigs. Conclusions: Concluding, this is the first report describing the comparative features of the mucous glands in the two rodents, with detailed histological and histochemical features.

scholarly journals 21. KAJIAN HISTOKIMIA SEBARAN KARBOHIDRAT PADA KELENJAR MANDIBULARIS DAN KELENJAR LINGUALIS AYAM PETELUR (Gallus sp.) Histochemical Study of Mandibular and Lingual Glands Carbohydrate Distribution in Layer (Gallus sp.)

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Hamny H
Keyword(s):  
Key Words ◽  
Histochemical Study ◽  
Periodic Acid ◽  
Age Groups ◽  
Mandibular Gland ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Periodic Acid Schiff ◽  
Weak Reaction ◽  
Strong Intensity

The objective of this study was to investigate the distribution of carbohydrate in the mandibular and lingual glands of layer in difference age groups histochemically.The study was observed the mandibular and lingual glands from nine layers which grouped into three groups namely starter aged 40 days old, productive aged seven months old, and post-productive aged 1.5 years old. Each group consisted of three layers. A small piece of mandibular and lingual glands were fixed in Bouin solution for subsequent processed into histological preparation.The distribution of carbohydrate in both of glands detected by alcian blue staining (AB) pH 2.5 and periodic acid schiff (PAS). The result showed that mandibular and lingual glands of the three group layers reacted positively to AB pH 2.5 and PAS which found in the cytoplasm of cell and in the secretion on lumen. In the cytoplasm, the mandibular and lingualis reacted positively to AB pH 2.5 and PAS with the intensity ranging from weak reaction(+) to strong (+++), while in the lumen of glands showed negative reaction (-) to positive with the intensity of weak (+) to strong (+++). In mandibular gland, the acid carbohydrates were found with strong intensity (+++) in the productive and post-productive layer, while neutral carbohydrates were found with the same intensity on starter, productive and post-productive layers. In the lingual gland, the acid carbohydrates found with strong intensity in the three groups of layers while the neutral were found with strong intensity (+++) in the starter and post-productive layer. Positive reaction to AB pH 2.5 and PAS indicated that both of the salivary glands contain the neutral and acid carbohydrate. ____________________________________________________________________________________________________________________ Key words: mandibular gland, lingual gland, acid carbohydrate, neutral carbohydrate, layer

scholarly journals Histochemical Study of Cutaneous Mucins in Hypothyroid Dogs

1995 ◽  
Vol 32 (6) ◽  
pp. 628-634 ◽  
Author(s):  
S. Doliger ◽  
M. Delverdier ◽  
J. Moré ◽  
L. Longeart ◽  
A. Régnier ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Connective Tissue ◽  
Alcian Blue ◽  
Histochemical Study ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Pas Reaction ◽  
Skin Biopsies ◽  
Hematoxylin And Eosin

A dermal mucinoisis, visualized as dermal alcianophilic material, is occasionally present in canine hypothyroidism (myxedema). Various histochemical reactions (alcian blue/periodic acid–Schiff [PAS], alcian blue at pH 2.6, alcian blue at pH 1.0, critical electrolytical concentrations with and without dimethylsulfoxide, differential hydrolysis by hyaluronidases) were performed on skin biopsies from six dogs (four females and two males ranging from 8 to 13 years) affected by hypothyroidism, all of them presenting dermal mucinosis in hematoxylin and eosin–stained sections. In these dogs, the only polysaccharide compound involved in the dermal mucinosis was hyaluronic acid. In this study, hyaluronic acid dermal deposits of hypothyroid dogs were significantly different from those of controls in subepidermal connective tissue and loose reticular connective tissue but not in periadnexal zones. We recommend the combined alcian blue/PAS reaction as a routine technique to assess dermal mucinosis in hypothyroid dogs.

Histologic and Histochemical Characterization of Seminal Vesicle Intraluminal Secretions

2001 ◽  
Vol 125 (1) ◽  
pp. 141-145
Author(s):  
Rajal B. Shah ◽  
Min W. Lee ◽  
Alvaro A. Giraldo ◽  
Mahul B. Amin
Keyword(s):  
Seminal Vesicle ◽  
Alcian Blue ◽  
Prostate Carcinoma ◽  
Periodic Acid ◽  
Prostate Gland ◽  
Seminal Vesicles ◽  
Periodic Acid Schiff ◽  
Associated Malignancy ◽  
Distinguishing Features

Abstract Context.—We have observed intraluminal crystalloid morphology in seminal vesicles that is superficially similar to that seen in prostate neoplasia, but found little information on such morphology in the literature. Design.—Two hundred fifty-three prostate specimens (163 needle biopsies, 75 radical prostatectomies with prostate carcinoma, 11 prostates from autopsy, and 4 cystoprostatectomies without prostate carcinoma) were examined for seminal vesicle secretions, which were categorized as (a) dense platelike inspissated, (b) fluidlike, (c) crystalloid morphology, and (d) absent. Histochemical stains (periodic acid–Schiff with and without diastase, Alcian blue at pH 2.5, and mucicarmine) were performed to characterize the nature of secretions. Results.—Proteinaceous secretions were identified in 82% of seminal vesicles examined. Of these, 61% had predominantly dense, platelike, inspissated secretions, 15% had predominantly fluidlike secretions, and 24% had predominantly crystalloid morphology. Although in some cases the crystalloid morphology resembled that of prostatic intraluminal crystalloids, the seminal vesicle crystalloids differed in that they were invariably multiple, had curved edges, and had varied forms (elliptical, cylindrical, rodlike, and rectangular). Seventy-one percent of seminal vesicle crystalloids were associated with dense, platelike, inspissated secretions and appeared to be created by fracturing within platelike secretions. There was no relationship between seminal vesicle crystalloid morphology and associated malignancy in the prostate gland, as it was seen in 24% of cases with prostate carcinoma and 25% of cases without prostate carcinoma (P = 1.0000). Fluidlike secretions were positive for Alcian blue (pH 2.5) and mucicarmine, whereas dense platelike secretions and crystalloid morphology were negative for Alcian blue (pH 2.5) and mucicarmine. Conclusions.—Seminal vesicle secretions are fairly common and, when fluidlike, are composed of acid mucopolysaccharides. Inspissation of secretions appears to be associated with loss of acidity, presumably resulting in dense platelike secretions and crystallization. Awareness of both the crystalloid morphology in seminal vesicle tissue and the distinguishing features from prostatic crystalloids may be important while interpreting prostate needle biopsies in which seminal vesicle epithelium may be confused for prostate carcinoma because of a small acinar morphology with accompanying cytologic atypia and crystalloid morphology.

scholarly journals Evaluation of Better Staining Method among Hematoxylin and Eosin, Giemsa and Periodic Acid Schiff-Alcian Blue for the Detection of Helicobacter pylori in Gastric Biopsies

2020 ◽  
Vol 27 (5) ◽  
pp. 53-61
Author(s):  
Abdullah Saleh Alkhamiss
Keyword(s):  
Helicobacter Pylori ◽  
Alcian Blue ◽  
National Guard ◽  
Periodic Acid ◽  
Giemsa Stain ◽  
Periodic Acid Schiff ◽  
Gastric Biopsies ◽  
Hematoxylin And Eosin ◽  
H Pylori ◽  
Sensitivity Specificity

Background: This study was undertaken to evaluate the preferred method (Giemsa or periodic acid Schiff-Alcian blue [PAS-AB] stains) of detecting Helicobacter pylori (H. pylori) in gastric mucosal biopsies in terms of sensitivity, specificity and applicability. To the best of my knowledge, this is the first report comparing Giemsa and PAS-AB staining for the detection of H. pylori in such biopsies. Methods: The formalin-fixed paraffin-embedded blocks of 49 gastric biopsies from different patients were collected from the archive of anatomical pathology at King Abdulaziz Medical City, National Guard, Riyadh, Saudi Arabia. From each block, three slides were prepared and analysed using the hematoxylin and eosin (H&E), Giemsa and PAS-AB stains to detect the presence/absence of H. pylori, and the results were compared in terms of sensitivity, specificity and applicability. Results: The majority of the biopsies in this study showed antrum-type gastric mucosa. Only 15 biopsies showed active gastritis, whereas the rest showed chronic gastritis. Three biopsies showed intestinal metaplasia. All were detected by PAS-AB stain, but only two-thirds were detected by H&E stain. Fifteen gastric biopsies showed H. pylori infection in general and in 13 of them, active gastritis cases were discovered. Fourteen out of these 15 H. pylori infection cases were detected by Giemsa stain, whereas only 13 cases were detected by H&E stain. PAS-AB stain showed the worst results since it demonstrated only 40% sensitivity and 67.65% specificity in H. pylori detection. Conclusion: Giemsa stain has better sensitivity and specificity in gastric H. pylori infection detection than PAS-AB. Therefore, using PAS-AB stain to detect H. pylori infection is not recommended.

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