scholarly journals GROWTH PERFORMANCE OF TRANSFECTION TIGER SHRIMP BROODSTOCK Penaeus monodon IN DIFFERENT GENERATION

2017 ◽  
Vol 9 (1) ◽  
pp. 185-199 ◽  
Author(s):  
Hidayat Suryanto Suwoyo ◽  
. Sahabuddin

Diseases resistant genes assemblage for tiger shrimp has been initiated by The Research Institute for Coastal Aquaculture in collaboration with Bogor Agricultural Institute, through transgenesis approach under anti-virus genes transfection. The  study aimed to evaluate the growth performance of broodstock candidates of tiger shrimp at different generati on (F0 and F1). This research was  conducted at 2000 m2size of four ponds in Takalar Regency, South Sulawesi..  The treatment was  different generations of broodstocks, which were: broodstocks originated from F0generation, (A) and F1 (B). The 22.63 to 28.57 g of broodstock candidates were stocked 0.5 ind.m-2 and then reared for 128 days. During rearing period, these shrimp were fed usingcommercial pelleted feed with content 36-38% of protein in dosage of 10-4%/  body weight. Feeding frequency was applied in the morning and in the evening. Measured variables were growth, size distribution, survival rate  and water quality The results indicated that the performances of these shrimps, growth, size distributions as well as survival rates between these F0 and F1 were not significantly different (p>0.05). The growth pattern was relatively equal between treatment  during rearing period. Survival rate of tiger  shrimp in this study ranged from 51.7 to 73.35%. This study have implications on the provision of superior broodstock shrimp in ponds in order to support the sustainability of shrimp seed production in hatchery. Keywords: broodstock, production, transgenic,  tiger shrimp

2017 ◽  
Vol 9 (1) ◽  
pp. 185
Author(s):  
Hidayat Suryanto Suwoyo ◽  
. Sahabuddin

<p><em>Diseases resistant genes assemblage for </em><em>tiger shrimp has been initiated by </em><em>The Research Institute for Coastal Aquaculture </em><em>i</em><em>n co</em><em>llabo</em><em>ration with Bogor Agricultural Institute</em><em>, </em><em>through</em><em> </em><em>transgenesis</em><em> approach under </em><em>anti-virus gene</em><em>s</em><em> transfection. The  study aim</em><em>ed</em><em> to evaluate the growth </em><em>performance of broodstock candidates of tiger shrimp </em><em>at different generati on (F<sub>0</sub> and F<sub>1</sub>). This research was  conducted at </em><em>2000 m<sup>2</sup>size </em><em>of four po</em><em>nds</em><em> in Takalar Regency, South Sulawesi</em><em>.</em><em><sup>.  </sup></em><em>T</em><em>he treatment</em><em> was</em><em>  differen</em><em>t</em><em> generations </em><em>of </em><em>broodstocks, which were: broodstocks originated from F<sub>0</sub></em><em></em><em>generation</em><em>,</em><em> (A) and F<sub>1</sub> (B)</em><em>. The 22.63 to 28.57 g of broodstock candidates were stocked </em><em>0.5 ind</em><em>.</em><em>m</em><em><sup>-</sup></em><em><sup>2</sup></em><em> and then reared for 128</em><em> days. During rearing period</em><em>, these</em><em> shrimp </em><em>were </em><em>fed</em><em> usingcommercial </em><em>pelle</em><em>ted feed</em><em> with content 36-38% of protein in dosage of 10-4%/  </em><em>body weight. Feeding frequency was </em><em>applied </em><em>in the morning and in the evening.</em><em> </em><em>Measured variables were </em><em>growth, size distribution, survival rate  and w</em><em>ater </em><em>quality </em><em>T</em><em>he results indicated that the performances of these shrimps, growth, size distributions as well as survival rates between these F<sub>0</sub> and F<sub>1</sub> were not significantly different (p&gt;0.05).</em><em> </em><em>The growth pattern was relatively equal between treatment  during rearing period. Survival rate of tiger  shrimp in this study ranged from 51.7 to 73.35%. This study have implications on the provision of superior broodstock shrimp in ponds in order to support the sustainability of shrimp seed production in hatchery.</em><em></em></p><p><strong> </strong></p><strong><em>Keywords: </em></strong><em>broodstock, production, transgenic,  tiger shrimp</em>


2021 ◽  
Vol 890 (1) ◽  
pp. 012038
Author(s):  
Suwardi ◽  
H S Suwoyo

Abstract Tiger shrimp (Penaeus monodon Fab.) is one of important commercial species in Indonesia. Low quality of shrimp fry due to the lack of good quality broodstock availability is shrimp cultivation problem that need to be solved. The study was aimed to evaluate growth and survival rate of broodstock candidate of transfection and non-transfection tiger shrimp. The study was conducted using four ponds with 2000 m2 in size in Takalar Regency. South Sulawesi. The treatment were A = transfection tiger shrimp broodstock candidate and B = non-transfection tiger shrimp broodstock candidate. Approximately 19.0-21.9 g/ind of shrimp were reared in 60 days in 0.25 individu/m2 (500 individu/2000 m2) stocking density. Commercial pelleted feed which content 38% of protein was fed three times a day in 5-3% of body weight dosage. Measured variables were growth performance, size distribution and survival rate. The result showed that shrimp growth were no significantly different (P>0.05), while survival rate and size distribution were significantly different (P<0.05). The dominant size of transfection shrimp was 41-50 g/ind. Whereas, non-transfection shrimp was dominated by 31-40 g/ind in size (48.39%). Survival rate of tiger shrimp broodstock candidate in this study ranged from 30.80 to 64.10%.


2021 ◽  
Vol 16 (1) ◽  
pp. 45
Author(s):  
Andi Akhmad Mustafa ◽  
Andi Indra Jaya Asaad ◽  
Dominggus Linthin

Salah satu program dari Gubernur Sulawesi Selatan periode 2018-2023 adalah mengembalikan kejayaan udang windu di Sulawesi Selatan. Telah dilakukan kegiatan yang bertujuan mengetahui performa budidaya udang windu di tambak pembudidaya yang dilaksanakan pada musim kemarau tahun 2019 di Kecamatan Marusu Kabupaten Maros. Kegiatan budidaya udang windu teknologi tradisional plus dilaksanakan di tambak Dusun Kurilompo, Desa Nisombalia Kecamatan Marusu Kabupaten Maros. Sebanyak enam petak tambak pembudidaya ditebari tokolan udang windu dengan padat penebaran dari 10.000 sampai 30.000 ekor/ha. Setelah dipelihara selama 69 sampai 80 hari dilakukan panen dan selanjutnya dilakukan penentuan sintasan, bobot rata-rata, produksi udang windu, dan rasio konversi pakan (RKP). Data yang diperoleh dianalisis secara deskriptif. Hasil kegiatan menunjukkan bahwa kualitas air di tambak Kecamatan Marusu, Kabupaten Maros pada musim kemarau dicirikan dengan salinitas yang berkisar antara 36,63-69,40 ppt; suatu kondisi yang tidak optimal untuk budidaya udang windu. Pada saat panen didapatkan sintasan udang windu dari 27,57% sampai 66,67%; bobot rata-rata dari 14,29-40,00 g/ekor; produksi dari 90,1-800,0 kg/ha/musim; dan RKP dari 0,54:1 sampai 2,10:1. Disimpulkan bahwa budidaya udang windu di tambak bersalinitas tinggi di daerah ini merupakan penyebab utama rendahnya sintasan, bobot rata-rata, produksi udang windu, dan RKP. Direkomendasikan bahwa perbaikan budidaya udang dengan menjaga tingkat salinitas dalam nilai optimal harus disosialisasikan kepada pembudidaya udang windu di daerah tersebut.One of the main aquaculture development targeted by the South Sulawesi Province for the period 2018-2023 is to restore the glory of tiger shrimp farming in South Sulawesi. The recent study was carried out to uncover redevelopment opportunities of tiger shrimp culture and determine the performance of tiger shrimp culture in the farmers’ ponds. The study was performed during the dry season in Marusu Subdistrict Maros District, in 2019. Traditional plus tiger shrimp farming activities were carried out in the ponds of Kurilompo Subvillage, Nisombalia Village, Marusu Subdistrict, Maros District. A total of six brackishwater ponds were stocked with tiger shrimp fingerling with stocking densities of 10,000-30,000 ind./ha. After being cultured for 69 to 80 days, the shrimp were harvested and measured their survival rate, average weight, production of tiger shrimp, and feed conversion ratio (FCR). The data obtained were analyzed with descriptive statistics. The results showed that water quality in brackishwater ponds of Marusu Subdstrict Maros District, in the dry season is characterized by salinity ranging from 36.63 to 69.40 ppt, a condition that is not optimal for tiger shrimp culture. At harvest, the survival rate, average weight, production, and FCR of the farmed average tiger shrimp were 27.57%-66.67%, 14.29-40.00 g/ind., 90.1-800.0 kg/ha/season, and 0.54:1-2.10:1, respectively. It is concluded that the culture of tiger shrimp in the brackishwater ponds with high salinity in this area is the primary cause of poor survival rate, average weight, production of tiger shrimp, and FCR. It is recommended that culture shrimp improvement via maintaining salinity level within the optimal values has to be disseminated to the fish farmers in the area.


2012 ◽  
Vol 21 (3) ◽  
pp. 655-666 ◽  
Author(s):  
Subuntith Nimrat ◽  
Phattanunt Tanutpongpalin ◽  
Kallaya Sritunyalucksana ◽  
Traimat Boonthai ◽  
Verapong Vuthiphandchai

2013 ◽  
Vol 46 (7) ◽  
pp. 1668-1678 ◽  
Author(s):  
Jin Niu ◽  
Chun-Hou Li ◽  
Li-Xia Tian ◽  
Yong-Jian Liu ◽  
Xu Chen ◽  
...  

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9646
Author(s):  
Tanaporn Uengwetwanit ◽  
Umaporn Uawisetwathana ◽  
Sopacha Arayamethakorn ◽  
Juthatip Khudet ◽  
Sage Chaiyapechara ◽  
...  

Understanding the correlation between shrimp growth and their intestinal bacteria would be necessary to optimize animal’s growth performance. Here, we compared the bacterial profiles along with the shrimp’s gene expression responses and metabolites in the intestines between the Top and the Bottom weight groups. Black tiger shrimp (Penaeus monodon) were collected from the same population and rearing environments. The two weight groups, the Top-weight group with an average weight of 36.82 ± 0.41 g and the Bottom-weight group with an average weight of 17.80 ± 11.81 g, were selected. Intestines were aseptically collected and subjected to microbiota, transcriptomic and metabolomic profile analyses. The weighted-principal coordinates analysis (PCoA) based on UniFrac distances showed similar bacterial profiles between the two groups, suggesting similar relative composition of the overall bacterial community structures. This observed similarity was likely due to the fact that shrimp were from the same genetic background and reared under the same habitat and diets. On the other hand, the unweighted-distance matrix revealed that the bacterial profiles associated in intestines of the Top-weight group were clustered distinctly from those of the Bottom-weight shrimp, suggesting that some unique non-dominant bacterial genera were found associated with either group. The key bacterial members associated to the Top-weight shrimp were mostly from Firmicutes (Brevibacillus and Fusibacter) and Bacteroidetes (Spongiimonas), both of which were found in significantly higher abundance than those of the Bottom-weight shrimp. Transcriptomic profile of shrimp intestines found significant upregulation of genes mostly involved in nutrient metabolisms and energy storage in the Top-weight shrimp. In addition to significantly expressed metabolic-related genes, the Bottom-weight shrimp also showed significant upregulation of stress and immune-related genes, suggesting that these pathways might contribute to different degrees of shrimp growth performance. A non-targeted metabolome analysis from shrimp intestines revealed different metabolic responsive patterns, in which the Top-weight shrimp contained significantly higher levels of short chain fatty acids, lipids and organic compounds than the Bottom-weight shrimp. The identified metabolites included those that were known to be produced by intestinal bacteria such as butyric acid, 4-indolecarbaldehyde and L-3-phenyllactic acid as well as those produced by shrimp such as acyl-carnitines and lysophosphatidylcholine. The functions of these metabolites were related to nutrient absorption and metabolisms. Our findings provide the first report utilizing multi-omics integration approach to investigate microbiota, metabolic and transcriptomics profiles of the host shrimp and their potential roles and relationship to shrimp growth performance.


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